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Elucidation of the mechanisms of substance-selective introduction into bacterial outer membrane vesicles

Research Project

Project/Area Number 17K19551
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Research Field Pathology, Infection/Immunology, and related fields
Research InstitutionOsaka University (2018)
The University of Tokyo (2017)

Principal Investigator

Mimuro Hitomi  大阪大学, 微生物病研究所, 准教授 (80396887)

Project Period (FY) 2017-06-30 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2018: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2017: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Keywords細菌外膜小胞 / sRNA / 細菌 / 核酸
Outline of Final Research Achievements

We have analyzed whole genome sequence and RNA-seq of the experimental strain of Helicobacter pylori and found that H. pylori showed different expression patterns of bacterial small RNA (sRNA) in the outer membrane vesicles (OMVs) compared with that in whole bacterial cells. We identified an sRNA, which presented in relatively large amounts in OMV compared with whole bacterial cells. Furthermore, we identified a bacterial protein directly bound to the sRNA. Based on the analysis of the phenotype of the sRNA-deletion mutant, we found possibilities that the sRNA controlled the amounts of OMV. Moreover, we found the protein, which directly bound to the sRNA, was essential for the existence of the bacteria.

Academic Significance and Societal Importance of the Research Achievements

本研究の結果、菌体sRNAを選択的にOMV内へ封入するメカニズムの存在が明らかとなり、OMV量の制御に関与するsRNAを同定した。今後、菌体がその生理的目的に応じてOMV内容物を能動的に変動させる分子機構が明らかになれば、新たな細菌生理学や細菌病原性研究の展開が可能となる。さらには、選択的OMV封入機構を利用した、新たな画期的ドラッグデリバリー技術への応用が期待できる。

Report

(3 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • Research Products

    (14 results)

All 2019 2018 2017

All Journal Article (10 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 10 results,  Open Access: 5 results,  Acknowledgement Compliant: 1 results) Presentation (4 results)

  • [Journal Article] GPR31-dependent dendrite protrusion of intestinal CX3CR1+ cells by bacterial metabolites.2019

    • Author(s)
      Morita N, Umemoto E, Fujita S, Hayashi A, Kikuta J, Kimura I, Haneda T, Imai T, Inoue A, Mimuro H, Maeda Y, Kayama H, Okumura R, Aoki J, Okada N, Kida T, Ishii M, Nabeshima R, Takeda K
    • Journal Title

      nature

      Volume: 566 Issue: 7742 Pages: 110-114

    • DOI

      10.1038/s41586-019-0884-1

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Thiamin transport in Helicobacter pylori lacking the de novo synthesis of thiamin2019

    • Author(s)
      Nosaka K, Uchiyama R, Tadano K, Endo Y, Hayashi M, Konno H, Mimuro H
    • Journal Title

      Microbiology

      Volume: 165 Issue: 2 Pages: 224-232

    • DOI

      10.1099/mic.0.000765

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Shigella effector IpaH4.5 targets 19S regulatory subunit RPN13 in the 26S proteasome to dampen cytotoxic T lymphocyte activation.2019

    • Author(s)
      Otsubo R, Mimura H, Ashida H, Hamazaki J, Murata S, Sasakawa C.
    • Journal Title

      Cell Microbiol.

      Volume: 21 Issue: 3 Pages: e12974-e12974

    • DOI

      10.1111/cmi.12974

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Molecular basis for governing the morphology of type-I collagen fibrils by Osteomodulin2018

    • Author(s)
      Takumi Tashima , Satoru Nagatoishi, Jose M.M. Caaveiro, Makoto Nakakido, Hiroshi Sagara, Osamu Kusano-Arai, Hiroko Iwanari, Hitomi Mimuro, Takao Hamakubo, Shin-ichi Ohnuma and Kouhei Tsumoto
    • Journal Title

      Communications Biology

      Volume: 1:33 Issue: 1 Pages: 1-10

    • DOI

      10.1038/s42003-018-0038-2

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Helicobacter pylori induces somatic mutations in TP53 via overexpression of CHAC1 in infected gastric epithelial cells2018

    • Author(s)
      Wada Yuriko、Takemura Kosuke、Tummala Padmaja、Uchida Keisuke、Kitagaki Keisuke、Furukawa Asuka、Ishige Yuuki、Ito Takashi、Hara Yukichi、Suzuki Takashige、Mimuro Hitomi、Board Philip G.、Eishi Yoshinobu
    • Journal Title

      FEBS Open Bio

      Volume: 8 Issue: 4 Pages: 671-679

    • DOI

      10.1002/2211-5463.12402

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Monotherapy with a novel intervenolin derivative, AS-1934, is an effective treatment for Helicobacter pylori infection2018

    • Author(s)
      Ohishi Tomokazu、Masuda Tohru、Abe Hikaru、Hayashi Chigusa、Adachi Hayamitsu、Ohba Shun-ichi、Igarashi Masayuki、Watanabe Takumi、Mimuro Hitomi、Amalia Eri、Inaoka Daniel Ken、Mochizuki Kota、Kita Kiyoshi、Shibasaki Masakatsu、Kawada Manabu
    • Journal Title

      Helicobacter

      Volume: 23 Issue: 2

    • DOI

      10.1111/hel.12470

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Characterization of morphological conversion of Helicobacter pylori under anaerobic conditions2018

    • Author(s)
      Hirukawa Sayaka、Sagara Hiroshi、Kaneto Satoshi、Kondo Tomoyo、Kiga Kotaro、Sanada Takahito、Kiyono Hiroshi、Mimuro Hitomi
    • Journal Title

      Microbiology and Immunology

      Volume: 62 Issue: 4 Pages: 221-228

    • DOI

      10.1111/1348-0421.12582

    • NAID

      40021538387

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Shigella hijacks the glomulin?cIAPs?inflammasome axis to promote inflammation2018

    • Author(s)
      Suzuki Shiho、Suzuki Toshihiko、Mimuro Hitomi、Mizushima Tsunehiro、Sasakawa Chihiro
    • Journal Title

      EMBO Rep

      Volume: 19 Issue: 1 Pages: 89-101

    • DOI

      10.15252/embr.201643841

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Resveratrol strongly enhances the retinoic acid-induced superoxide generating activity via up-regulation of gp91-phox gene expression in U937 cells2018

    • Author(s)
      Kikuchi Hidehiko、Mimuro Hitomi、Kuribayashi Futoshi
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 495 Issue: 1 Pages: 1195-1200

    • DOI

      10.1016/j.bbrc.2017.11.161

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Epidemiological study on gastric Helicobacter spp. in dogs with gastrointestinal diseases in Japan: Diversity of H. heilmannii s.s.2017

    • Author(s)
      〇Sanae Kubota-Aizawa, Koichi Ohno, Kenjiro Fukushima, Hideyuki Kanemoto, Ko Nakashima, Kazuyuki Uchida, James K. Chambers, Yuko Goto-Koshino, Takayasu Watanabe, Tsutomu Sekizaki, Hitomi Mimuro, Hajime Tsujimoto
    • Journal Title

      The Veterinary Journal

      Volume: 印刷中 Pages: 56-62

    • DOI

      10.1016/j.tvjl.2017.04.004

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] ハイパーミューテーターを用いたピロリ菌持続感染メカニズムの解析2018

    • Author(s)
      三室 仁美
    • Organizer
      第24回日本ヘリコバクター学会学術集会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Helicobacter pylori 持続感染における菌体表面糖鎖変動メカニズムの解析2018

    • Author(s)
      黒田 英介,小椋 義俊,林 哲也,三室 仁美
    • Organizer
      第91回日本細菌学会総会
    • Related Report
      2017 Research-status Report
  • [Presentation] Functional variation of BabA on persistent infection by Helicobacter pylori2017

    • Author(s)
      Eisuke Kuroda, Hitomi Mimuro
    • Organizer
      第23回日本ヘリコバクター学会学術集会
    • Related Report
      2017 Research-status Report
  • [Presentation] Implications for H. pylori anaerobic cultures as prophylactic vaccine antigens2017

    • Author(s)
      Sayaka Hirukawa, Hitomi Mimuro
    • Organizer
      第23回日本ヘリコバクター学会学術集会
    • Related Report
      2017 Research-status Report

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Published: 2017-07-21   Modified: 2020-03-30  

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