Regulatory mechanisms for microenvironment and metastasis of cancers with glycosphigolipids via extracellular vesicles
Project/Area Number |
17K19616
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Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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Allocation Type | Multi-year Fund |
Research Field |
Tumor biology and related fields
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Research Institution | Chubu University |
Principal Investigator |
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Project Period (FY) |
2017-06-30 – 2020-03-31
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Project Status |
Completed (Fiscal Year 2019)
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Budget Amount *help |
¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2018: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2017: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
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Keywords | エクソソーム / 糖脂質 / ガングリオシド / インテグリン / GD3 / 細胞膜 / EGF受容体 / 細胞外顆粒 / 癌微小環境 / エキソソーム / 微小環境 / 癌転移 / 脂質ラフト |
Outline of Final Research Achievements |
Using cancer-associated glycolipid-expressing cell lines and their normal counterparts (control), secreted amounts of exosomes and levels of contained glycolipids and associated membrane proteins were analyzed. Consequently, in multiple cell line systems, exosomes derived from GD3-expressing cell lines expressed much higher amounts of integrin isoforms than those from individual GD3-negative controls. There were no definite differences in the integrin expression levels in the cell lysates between GD3-positive and GD3-negative cell lines. In the analysis of EGF receptor levels as measured similarly, they showed increase in GD3-expressing cell lines in both cell lysates and secreted exosomes. Now, we are analyzing composition of glycosphingolipids in exosomes, and also their functions.
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Academic Significance and Societal Importance of the Research Achievements |
癌関連糖脂質が、腫瘍マーカーとしてのみならず、癌細胞の悪性形質に重要な役割を果たすことが示されてきたが、本研究では、癌関連糖脂質を発現する細胞由来の細胞外分泌顆粒であるエクソソームが、接着受容体であるインテグリンを著明に多く含んでいることが判明した。このことから、癌細胞の浸潤、転移等の悪性形質において、細胞膜表面のみならず、癌関連糖脂質とインテグリンがエクソソームにおいても会合して、悪性形質に重要な役割を果たしていることが示唆された。とくに転移の予防においてエクソソームが標的になりうることを示唆する結果である。
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Report
(4 results)
Research Products
(47 results)
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[Journal Article] Novel molecular mechanisms for roles of gangliosides in the nervous system elucidated by genetic engineering.2020
Author(s)
Furukawa, K., Ohmi, Y., Yesmin, F., Tajima, O., Kondo, Y., Pu Zhang, P., Hashimoto, N., Ohkawa, Y., Bhuiyan, R.H., Furukawa, K.
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Journal Title
Int. J. Mol. Sci.
Volume: 21
Issue: 6
Pages: 1906-1906
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Cholera toxin B induces interleukin-1β production from resident peritoneal macrophages through the pyrin inflammasome as well as the NLRP3 inflammasome2019
Author(s)
Orimo T, Sasaki I, Hemmi H, Ozasa T, Fukuda-Ohta Y, Ohta T, Morinaka M, Kitauchi M, Yamaguchi T, Sato Y, Tanaka T, Hoshino K, Katayama KI, Fukuda S, Miyake K, Yamamoto M, Satoh T, Furukawa K, Kuroda E, Ishii KJ, Takeda K, Kaisho T
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Journal Title
International Immunology
Volume: 印刷中
Issue: 10
Pages: 657-668
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Deficiency of GD3 Synthase in Mice Resulting in the Attenuation of Bone Loss with Aging2019
Author(s)
Yo S, Hamamura K, Mishima Y, Hamajima K, Mori H, Furukawa K, Kondo H, Tanaka K, Sato T, Miyazawa K, Goto S, Togari A
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Journal Title
Int. J. Mol. Sci.
Volume: 10
Issue: 11
Pages: 2825-2825
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Genome-wide CRISPR screens for Shiga toxins and Ricin reveal Golgi proteins critical for glycosylation.2018
Author(s)
Songhai Tian, Khaja Muneeruddin, Mei Yuk Choi, Liang Tao, Robiul H. Bhuiyan, Yuhsuke Ohmi, Keiko Furukawa, Koichi Furukawa, Sebastian Boland, Scott A. Shaffer, Rosalyn M. Adams, Min Dong
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Journal Title
PLoS Biol.
Volume: 16(11)
Issue: 11
Pages: 1-31
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] ASC amino acid transporter 2, defined by enzyme-mediated activation of radical sources, enhances malignancy of GD2-positive small-cell lung cancer.2018
Author(s)
Nobutoshi Esaki, Yuki Ohkawa, Noboru Hashimoto, Yuhsuke Tsuda, Yuhsuke Ohmi, Robiul H. Bhuiyan, Norihiro Kotani, Koichi Honke, Atsushi Enomoto, Masahide Takahashi, Keiko Furukawa, Koichi Furukawa.
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Journal Title
Cancer sci.
Volume: 109
Issue: 1
Pages: 141-153
DOI
Related Report
Peer Reviewed / Open Access
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