Development of human iPS cell-derived model for hepatocarcinogenesis

• Project/Area Number: 17K19647
• Research Category: Grant-in-Aid for Challenging Research (Exploratory)
• Allocation Type: Multi-year Fund
• Research Field: General internal medicine and related fields
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: Asahina Yasuhiro 東京医科歯科大学, 大学院医歯学総合研究科, 寄附講座教授 (00422692)
• Co-Investigator(Kenkyū-buntansha): 柿沼 晴 東京医科歯科大学, 大学院医歯学総合研究科, 寄附講座准教授 (30372444) ; 中川 美奈 東京医科歯科大学, 統合教育機構, 准教授 (30401342)
• Project Period (FY): 2017-06-30 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000); Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2018: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000); Fiscal Year 2017: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: ヒトiPS細胞 / 肝細胞癌 / HBV / 発癌モデル / 肝星細胞 / HBV integration / iPS細胞 / ＨＣＶ / ＨＢＶ

Outline of Final Research Achievements

The genetic alterations responsible for HCC development and progression are unclear. Moreover, elucidation of mechanism for hepatocarcinogenesis involving host-viral genomic factors is hampered by lack of an appropriate in vitro human disease model. In this study, details of viral integration into the host genome associated with hepatocellular carcinoma were clarified, and the basis for the development of human iPS cell-derived in vitro model was constructed for analysing the mechanism responsible for these host-virus chimeric genome.

Academic Significance and Societal Importance of the Research Achievements

本研究成果により、抗ウイルス療法によっても抑制できなかった肝発癌に対して、新たな対策を講じるための標的となる遺伝子要因が明らかとなった。また、ヒトiPS細胞を用いたin vitro解析モデル開発の技術的基盤を構築したことで、これまで未知であった癌化や病態形成のメカニズムが明らかとなる糸口が切り開かれた。さらに、本研究成果は、肝臓領域のみならず他のあらゆる臨床医学分野に応用可能であり、それぞれの分野における病態解明が加速することが期待される。

Research Products

• [Journal Article] JNJ-4178 (adafosbuvir, odalasvir, and simeprevir) in Japanese patients with chronic hepatitis C virus genotype 1 or 2 infection with or without compensated cirrhosis: The Phase IIa OMEGA-3 study. (2020)
  • Author(s): Takehara T, Chayama K, Kurosaki M, Yatsuhashi H, Tanaka Y, Hiramatsu N, Sakamoto N, Asahina Y, Nozaki A, Nakano T, Hagiwara Y, Shimizu H, Yoshida H, Huang Y, Biermer M, Vijgen L, Hayashi N.
  • Journal Title: J Gastroenterol Volume: － Issue: 6 Pages: 640-652
  • DOI: 10.1007/s00535-020-01672-0
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Time course alterations of virus sequences and immunoglobulin titers in a chronic hepatitis E patient (2020)
  • Author(s): Nitta Sayuri、Takahashi Kazuaki、Kawai‐Kitahata Fukiko、Tsuchiya Jun、Sato Ayako、Miyoshi Masato、Murakawa Miyako、Itsui Yasuhiro、Nakagawa Mina、Azuma Seishin、Kakinuma Sei、Watanabe Mamoru、Asahina Yasuhiro
  • Journal Title: Hepatology Research Volume: 50 Issue: 4 Pages: 524-531
  • DOI: 10.1111/hepr.13480
  • Peer Reviewed
• [Journal Article] Vasoactive Intestinal Peptide Derived From Liver Mesenchymal Cells Mediates Tight Junction Assembly in Mouse Intrahepatic Bile Ducts (2020)
  • Author(s): Sato A, Kakinuma S, Miyoshi M, Kamiya A, Tsunoda T, Kaneko S, Tsuchiya J, Shimizu T, Takeichi E, Nitta S, Kawai-Kitahata F, Murakawa M, Itsui Y, Nakagawa M, Azuma S, Koshikawa N, Seiki S, Nakauchi H, Asahina Y, Watanabe M.
  • Journal Title: Hepatology Communications Volume: 4 Issue: 2 Pages: 235-254
  • DOI: 10.1002/hep4.1459
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Sorafenib-Regorafenib Sequential Therapy in Japanese Patients with Unresectable Hepatocellular Carcinoma?Relative Dose Intensity and Post-Regorafenib Therapies in Real World Practice (2019)
  • Author(s): Wang、Tsuchiya、Kurosaki、Yasui、Inada、Kirino、Yamashita、Sekiguchi、Hayakawa、Osawa、Okada、Higuchi、Takaura、Maeyashiki、Kaneko、Tamaki、Nakanishi、Itakura、Takahashi、Asahina、Enomoto、Izumi
  • Journal Title: Cancers Volume: 11 Issue: 10 Pages: 1517-1517
  • DOI: 10.3390/cancers11101517
  • Peer Reviewed
• [Journal Article] Comprehensive genetic analysis of cholangiolocellular carcinoma with a coexistent hepatocellular carcinoma‐like area and metachronous hepatocellular carcinoma (2019)
  • Author(s): Kawai-Kitahata F, Asahina Y, Kaneko S, Tsuchiya J, Sato A, Miyoshi M, Tsunoda T, Inoue-Shinomiya E, Murakawa M, Nitta S, Itsui Y, Nakagawa M, Azuma S, Kakinuma S, Tanabe M, Sugawara E, Takemoto A, Ojima H, Sakamoto M, Muraoka M, Takano S, Maekawa S, Enomoto N and Watanabe M.
  • Journal Title: Hepatology Research Volume: 49 Issue: 12 Pages: 1466-1474
  • DOI: 10.1111/hepr.13403
  • Peer Reviewed / Open Access
• [Journal Article] Impact of novel NS5A resistance-associated substitutions of hepatitis C virus detected in treatment-experienced patients (2019)
  • Author(s): Nitta S, Asahina Y, Kato T, Tsuchiya J, Inoue-Shinomiya E, Sato A, Tsunoda T, Miyoshi M, Kawai-Kitahata F, Murakawa M, Itsui Y, Nakagawa M, Azuma S, Kakinuma S, Hikita H, Takehara T, Watanabe M.
  • Journal Title: Scientific Reports Volume: 9 Issue: 1 Pages: 5722-5722
  • DOI: 10.1038/s41598-019-42114-z
  • Peer Reviewed / Open Access
• [Journal Article] Loss of Fibrocystin Promotes Interleukin-8-Dependent Proliferation and CTGF Production of Biliary Epithelium. (2019)
  • Author(s): Tsunoda T, Kakinuma S, Miyoshi M, Kamiya A, Kaneko S, Sato A, Tsuchiya J, Nitta S, Kawai-Kitahata F, Murakawa M, Itsui Y, Nakagawa M, Azuma S, Sogo T, Komatsu H, Mukouchi R, Inui A, Fujisawa T, Nakauchi H, Asahina Y, Watanabe M.
  • Journal Title: Journal of Heptatology Volume: 印刷中 Issue: 1 Pages: 143-152
  • DOI: 10.1016/j.jhep.2019.02.024
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Combinations of two drugs among NS3/4A inhibitors, NS5B inhibitors and non-selective antiviral agents are effective for hepatitis C virus with NS5A-P32 deletion in humanized-liver mice (2019)
  • Author(s): Doi Akira、Hikita Hayato、Kai Yugo、Tahata Yuki、Saito Yoshinobu、Nakabori Tasuku、Yamada Ryoko、Kodama Takahiro、Sakamori Ryotaro、Murayama Asako、Nitta Sayuri、Asahina Yasuhiro、Suemizu Hiroshi、Tatsumi Tomohide、Kato Takanobu、Takehara Tetsuo
  • Journal Title: Journal of Gastroenterology Volume: 54 Issue: 5 Pages: 449-458
  • DOI: 10.1007/s00535-018-01541-x
  • Peer Reviewed
• [Journal Article] .Association of serum IFN-λ3 levels with hepatocarcinogenesis in chronic hepatitis C patients treated with direct-acting antiviral agents. (2019)
  • Author(s): Emi Inoue-Shinomiya, Miyako Murakawa, Yasuhiro Asahina, Mina Nakagawa, Jun Tsuchiya, Ayako Sato, Tomoyuki Tsunoda, Masato Miyoshi, Sayuri Nitta, Fukiko Kawai-Kitahata, Yasuhiro Itsui, Seishin Azuma, Sei Kakinuma, Kazumoto Murata, Masashi Mizokami, Mamoru Watanabe.
  • Journal Title: Hepatology Research Volume: 印刷中 Issue: 5 Pages: 500-511
  • DOI: 10.1111/hepr.13307
  • Peer Reviewed / Open Access
• [Journal Article] LIM homeobox 2 promotes interaction between human iPS-derived hepatic progenitors and iPS-derived hepatic stellate-like cells. (2019)
  • Author(s): Miyoshi M, Kakinuma S, Kamiya A, Tsunoda T, Tsuchiya J, Sato A, Kaneko S, Nitta S, Kawai-Kitahata F, Murakawa M, Itsui Y, Nakagawa M, Azuma S, Nakauchi H, Asahina Y, Watanabe M.
  • Journal Title: Scientific Reports Volume: 9 Issue: 1 Pages: 2072-2072
  • DOI: 10.1038/s41598-018-37430-9
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Diabetic Retinopathy as a Risk Factor Associated with the Development of Hepatocellular Carcinoma in Nonalcoholic Fatty Liver Disease. (2019)
  • Author(s): Azuma S, Asahina Y, Kakinuma S, Azuma K, Miyoshi M, Inoue E, Tsunoda T, Sato A, Kaneko S, Nagata H, Kawai-Kitahata F, Murakawa M, Nitta S, Itsui Y, Tomita M, Nakagawa M, Watanabe M.
  • Journal Title: Digestive Diseases Volume: 37 Issue: 3 Pages: 247-254
  • DOI: 10.1159/000493580
  • Peer Reviewed
• [Journal Article] Improved Bone Safety of Tenofovir Alafenamide Compared to Tenofovir Disoproxil Fumarate Over 2 Years in Patients With Chronic HBV Infection (2018)
  • Author(s): Seto Wai-Kay、Asahina Yasuhiro、Brown Todd T.、Peng Cheng-Yuan、Stanciu Carol、Abdurakhmanov Dzhamal、Tabak Fehmi、Nguyen Tuan T.、Chuang Wan-Long、Inokuma Tetsuro、Ikeda Fusao、Santantonio Teresa Antonia、Habersetzer Fran?ois、Ramji Alnoor、Lau Audrey H.、Suri Vithika、Flaherty John F.、Wang Hongyuan、et al.
  • Journal Title: Clinical Gastroenterology and Hepatology Volume: －
  • DOI: 10.1016/j.cgh.2018.06.023
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Ledipasvir-sofosbuvir for treating Japanese patients with chronic hepatitis C virus genotype 2 infection. (2018)
  • Author(s): Asahina Y, Itoh Y, Ueno Y, Matsuzaki Y, Takikawa Y, Yatsuhashi H, Genda T, Ikeda F, Matsuda T, Dvory-Sobol H, Jiang D, Massetto B, Osinusi AO, Brainard DM, McHutchison JG, Kawada N, Enomoto N.
  • Journal Title: Liver Int Volume: 印刷中 Issue: 9 Pages: 1552-1561
  • DOI: 10.1111/liv.13685
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Up-to-seven criteria as a useful predictor for tumor downstaging to within Milan criteria and Child-Pugh grade deterioration after the initial conventional TACE. (2018)
  • Author(s): Yasui Y, Tsuchiya K, Kurosaki M, Takeguchi T, Takeguchi Y, Okada M, Wang W, Kubota Y, Goto T, Komiyama Y, Higuchi M, Takaura K, Hayashi T, Takada H, Tamaki N, Nakanishi H, Itakura J, Takahashi Y, Asahina Y, Enomoto N, Himeno Y, Izumi N.
  • Journal Title: Hepatol Res Volume: 48 Issue: 6 Pages: 442-450
  • DOI: 10.1111/hepr.13048
  • Peer Reviewed
• [Journal Article] Association Between Alanine Aminotransferase Elevation And UGT1A1*6 Polymorphisms In Daclatasvir And Asunaprevir Combination Therapy For Chronic Hepatitis C. (2017)
  • Author(s): Maekawa S, Sato M, Kuratomi N, Inoue T, Suzuki Y, Tatsumi A, Miura M, Matsuda S, Muraoka M, Nakakuki N, Amemiya F, Takano S, Fukasawa M, Nakayama Y, Yamaguchi T, Sato T, Sakamoto M, Murakawa M, Nakagawa M, Asahina Y, Enomoto N.
  • Journal Title: J Gastroenterol Volume: 印刷中 Issue: 6 Pages: 780-786
  • DOI: 10.1007/s00535-017-1405-3
  • Peer Reviewed / Open Access
• [Journal Article] Effect of interferon-based and -free therapy on early occurrence and recurrence of hepatocellular carcinoma in chronic hepatitis C. (2017)
  • Author(s): Nagata H, Nakagawa M, Asahina Y, Sato A, Asano Y, Tsunoda T, Miyoshi M, Kaneko S, Otani S, Kawai-Kitahata F, Murakawa M, Nitta S, Itsui Y, Azuma S, Kakinuma S, Nouchi T, Sakai H, Tomita M, Watanabe M
  • Journal Title: J Hepatol Volume: 67 Issue: 5 Pages: 933-939
  • DOI: 10.1016/j.jhep.2017.05.028
  • Peer Reviewed / Open Access
• [Journal Article] Hepatic IFNL4 expression is associated with non-response to interferonbased therapy through the regulation of basal interferon-stimulated gene expression in chronic hepatitis C patients. (2017)
  • Author(s): Murakawa M, Asahina Y, Kawai-Kitahata F, Nakagawa M, Nitta S, Otani S, Nagata H, Kaneko S, Asano Y, Tsunoda T, Miyoshi M, Itsui Y, Azuma S, Kakinuma S, Tanaka Y, Iijima S, Tsuchiya K, Izumi N, Tohda S, Watanabe M.
  • Journal Title: J Med Virol Volume: in press Issue: 7 Pages: 1241-1247
  • DOI: 10.1002/jmv.24763
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] ITPA gene variation and ribavirin-induced anemia in patients with genotype 2 chronic hepatitis C treated with sofosbuvir plus ribavirin. (2017)
  • Author(s): Murakawa M, Asahina Y, Nagata H, Nakagawa M, Kakinuma S, Nitta S, Kawai-Kitahata F, Otani S, Kaneko S, Miyoshi M, Tsunoda T, Asano Y, Sato A, Itsui Y, Azuma S, Nouchi T, Furumoto Y, Asano T, Chuganji Y, Tohda S, Watanabe M.
  • Journal Title: Hepatol Res Volume: 印刷中 Issue: 11 Pages: 1212-1218
  • DOI: 10.1111/hepr.12867
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Genome-wide association study identifies TLL1 variant associated with development of hepatocellular carcinoma after eradication of hepatitis C virus. (2017)
  • Author(s): Matsuura K, Asahina Y, Tnaka Y, et al.
  • Journal Title: Gastroenterology Volume: 152 Pages: 1383-1394
  • Peer Reviewed
• [Journal Article] Hepatitis B Surface Antigen Reduction by Switching from Long-term Nucleoside/nucleotide Analog Administration to Pegylated Interferon. (2017)
  • Author(s): Tamaki N, Kurosaki M, Kusakabe A, Orito E, Joko K, Kojima Y, Kimura H, Uchida Y, Hasebe C, Asahina Y, Izumi N.
  • Journal Title: J Viral Hepat Volume: 印刷中 Issue: 8 Pages: 672-678
  • DOI: 10.1111/jvh.12691
  • Peer Reviewed
• [Journal Article] Bone Morphogenetic Protein-4 Modulates Proliferation and Terminal Differentiation of Fetal Hepatic Stem/Progenitor Cells. (2016)
  • Author(s): Goto F, Kakinuma S, Miyoshi M, Tsunoda T, Kaneko S, Sato A, Asano Y, Otani S, Azuma S, Nagata H, Kawai-Kitahata F, Murakawa M, Nitta S, Itsui Y, Nakagawa M, Asahina Y, Watanabe M.
  • Journal Title: Hepatol Res Volume: 印刷中 Issue: 9 Pages: 941-952
  • DOI: 10.1111/hepr.12823
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] Do DAA therapy result in an increased risk of hepatocarcinogenesis? (2019)
  • Author(s): Asahina Y.
  • Organizer: Asian Pacific Digestive Week 2019
  • Int'l Joint Research / Invited
• [Presentation] Prevention and risk for liver cancer after anti-viral therapy in patients with HBV/HCV. (2019)
  • Author(s): Asahina Y.
  • Organizer: JSH International Liver Conference 2019
  • Int'l Joint Research / Invited
• [Presentation] Comprehensive analysis of cancer gene mutations and viral integration in hepatocellular carcinoma arising from non-fibrotic liver. (2019)
  • Author(s): Asahina Y, Kawai-Kitahata F, et al.
  • Organizer: The 70th Annual Meeting of the American Association for the Study of Liver Disease
  • Int'l Joint Research
• [Presentation] The association of serum IFN-λ3 levels with liver fibrosis and hepatocarcinogenesis in chronic hepatitis C patients treated with direct-acting antiviral agents. (2019)
  • Author(s): Murakawa M, Inoue-Shinomiya E, Asahina Y, et al.
  • Organizer: The 54th annual meeting of the European association for the study of the liver
  • Int'l Joint Research
• [Presentation] Impact of HCV clearance on HCC development and patient survival: propensity score-matched analysis of an ongoing database of 2,173 CHC patients. (2019)
  • Author(s): Nakagawa M, Asahina Y, et al.
  • Organizer: The 54th annual meeting of the European association for the study of the liver
  • Int'l Joint Research
• [Presentation] 核酸アナログ治療中および非治療のB型慢性肝疾患症例における発癌寄与因子の解析． (2019)
  • Author(s): 村川美也子, 朝比奈靖浩, 渡辺守．
  • Organizer: 第55回日本肝臓学会総会
• [Presentation] C型肝炎ウイルス排除後の肝発がんに関わるリスク因子と病態の解析． (2019)
  • Author(s): 朝比奈靖浩, 村川 美也子, 中川 美奈．
  • Organizer: 第55回日本肝臓学会総会
• [Presentation] 部分的脾動脈塞栓術(PSE)による肝機能改善効果及び血管径からみた解析． (2019)
  • Author(s): 先田信哉, 朝比奈靖浩, 渡辺守．
  • Organizer: 第55回日本肝臓学会総会
• [Presentation] ウイルス制御下における肝発癌および長期予後 前向きコホートを用いたIFNベース治療とIFNフリー治療の比較検討． (2019)
  • Author(s): 中川美奈, 朝比奈靖浩, 村川美也子．
  • Organizer: 第55回日本肝臓学会総会
• [Presentation] C型慢性肝疾患SVR後のde novo発癌、再発癌の検討． (2019)
  • Author(s): 中川美奈, 朝比奈靖浩, 渡辺守．
  • Organizer: 第105回日本消化器病学会総会
• [Presentation] C型肝炎ウイルス(HCV)のNS5A領域における薬剤耐性関連変異(Resistance associated substitutions:RAS)が抗ウイルス薬効果に与える影響． (2019)
  • Author(s): 新田沙由梨, 朝比奈靖浩, 渡辺守．
  • Organizer: 第55回日本肝臓学会総会
• [Presentation] HCV-NS5A領域のP32欠失変異(P32del)とA92K変異がウイルス増殖と抗HCV薬感受性感受性に与える影響． (2019)
  • Author(s): 新田沙由梨, 朝比奈靖浩, 渡辺守．
  • Organizer: 第105回日本消化器病学会総会
• [Presentation] Increased risk of HCC following DAA: fact or fiction? / Follow up strategy after SVR in chronic hepatitis C. (2018)
  • Author(s): Asahina Y.
  • Organizer: Asian Pacific Digestive Week 2018
  • Int'l Joint Research / Invited
• [Presentation] Gene mutational profile and viral integration in hepatocellular carcinoma with or without HBV/HCV suppression. (2018)
  • Author(s): Asahina Y, Kitahata-Kawai F, Murakawa M, Nitta S, Nakagawa M, Kakinuma S, Watanabe M.
  • Organizer: The 69th Annual Meeting of the American Association for the Study of Liver Disease (AASLD The Liver Meeting 2018)
  • Int'l Joint Research
• [Presentation] The characteristic and the anti-HCV reagents susceptibility analysis of NS5A Resistance-Associated Substitutions (RAS) detected after DAA treatment failure patients. (2018)
  • Author(s): Nitta S, Kato T, Tuchiya J, Inoue-Shinomiya E, Sato A, Tsunoda T, Miyoshi M, Kawai-Kitahata F, Murakawa M, Istui Y, Azuma S, Nakagawa M, Kakinuma S, Asahina Y.
  • Organizer: The 69th Annual Meeting of the American Association for the Study of Liver Disease (AASLD The Liver Meeting 2018)
  • Int'l Joint Research
• [Presentation] Post-treatment M2BPGi level is useful for predicting HCC occurrence and recurrence after viral eradication in chronic hepatitis C patients. (2018)
  • Author(s): Nakagawa M, Asahina Y, Kawai-Kitahata F, Murakawa M, Nitta S, Itsui Y, Azuma S, Kakinuma S, Tomita M, Watanabe M and the Ochanomizu Liver Conference Study Group.
  • Organizer: The 69th Annual Meeting of the American Association for the Study of Liver Disease (AASLD The Liver Meeting 2018)
  • Int'l Joint Research
• [Presentation] The in vitro analysis of NS5A resistance-associated substitutions (RAS) observed in DAA treatment failure patients. (2018)
  • Author(s): Sayuri Nitta, Takanobu Kato, Jun Tuchiya, Emi Inoue-Shinomiya, Ayako Sato, Tomoyuki Tsunoda, Masato Miyoshi, Fukiko Kawai-Kitahata, Miyako Murakawa, Yasuhiro Istui, Seishin Azuma, Mina Nakagawa, Sei Kakinuma, Yasuhiro Asahina.
  • Organizer: 25th International Symposium on Hepatitis C Virus and Related Viruses
  • Int'l Joint Research
• [Presentation] HBV Reactivation and changes in interferon-stimulated gene expression during treatment of direct-acting antivirals for HCV: analyses in a novel in vitro model for HBV-HCV coinfection using human induced pluripotent stem cell-derived hepatic cells. (2018)
  • Author(s): Asahina Y, Kaneko S, Kakinuma S, Kamiya A, Miyoshi M, Tsunoda T, Inoue-Shinomiya E, Nitta S, Sato A, Asano Y, Nagata H, Kawai-Kitahata F, Murakawa M, Itsui Y, Nakagawa M, Azuma S, Watanabe M.
  • Organizer: The 53red annual meeting of the European association for the study of the liver (EASL The International Liver Congress 2018)
  • Int'l Joint Research
• [Presentation] Difference of gene mutational profile among viral- and non-viral HCC with or without prior HBV infection: Results of comprehensive deep sequencing analyses of cancer genes and HBV/AAV integration. (2018)
  • Author(s): Kawai-Kitahata F, Asahina Y, Kakinuma S, Murakawa M, Nitta S, Nagata H, Kaneko S, Inoue E, Miyoshi M, Tsunoda T, Sato A, Nakagawa M, Itsui Y, Azuma S, Tanaka S, Tanabe M, Maekawa S, Enomoto N, Watanabe M.
  • Organizer: The 53red annual meeting of the European association for the study of the liver (EASL The International Liver Congress 2018)
  • Int'l Joint Research
• [Presentation] 肝発癌の完全制御を目指したC型慢性肝疾患SVR後サーベイランスの検討． (2018)
  • Author(s): 中川美奈, 村川美也子, 朝比奈靖浩．
  • Organizer: 第42回日本肝臓学会東部会
• [Presentation] 肝間葉系細胞との細胞間相互作用を介した胆管形成の分子機序． (2018)
  • Author(s): 佐藤綾子, 柿沼晴, 朝比奈靖浩．
  • Organizer: 第42回日本肝臓学会東部会
• [Presentation] F0の背景肝から発症した肝細胞癌における癌関連遺伝子変異とviral integrationの検討． (2018)
  • Author(s): 北畑富貴子, 朝比奈靖浩, 渡辺守．
  • Organizer: 第42回日本肝臓学会東部会
• [Presentation] iPS細胞先天性肝線維症病態モデルによる胆管細胞異常に起因する肝線維化における免疫応答の解析． (2018)
  • Author(s): 角田知之, 柿沼晴, 朝比奈靖浩．
  • Organizer: 第42回日本肝臓学会東部会
• [Presentation] 免疫抑制状態によるE型肝炎慢性化症例の解析． (2018)
  • Author(s): 井津井康浩, 北畑富貴子, 新田沙由梨, 村川美也子, 中川美奈, 東正新, 柿沼晴, 朝比奈靖浩, 渡辺守．
  • Organizer: 第42回日本肝臓学会東部会
• [Presentation] B型慢性肝疾患に対する核酸アナログ治療例・未治療例における発癌背景因子の解析． (2018)
  • Author(s): 村川美也子, 朝比奈靖浩, 渡辺守．
  • Organizer: 第22回日本肝臓学会大会
• [Presentation] Direct acting antivirals(DAA)治療不成功で認められる新規NS5A resistant associated substitutions(RAS)の特性と各種抗HCV薬に対する感受性の全長ウイルスを用いた基礎的検討． (2018)
  • Author(s): 新田沙由梨, 朝比奈靖浩, 渡辺守．
  • Organizer: 第22回日本肝臓学会大会
• [Presentation] HCVウイルスおよび宿主変異がDAA治療後肝発癌に与える影響． (2018)
  • Author(s): 中川美奈, 朝比奈靖浩, 渡辺守．
  • Organizer: 第22回日本肝臓学会大会
• [Presentation] 癌関連遺伝子変異とウイルスのヒトゲノムへの挿入から検討する肝炎ウイルス制御下肝細胞癌の病態． (2018)
  • Author(s): 北畑富貴子, 朝比奈靖浩, 渡辺守．
  • Organizer: 第54回日本肝臓学会総会
• [Presentation] 日本人のジェノタイプ2型C型慢性肝炎患者に対するLDV/SOF療法． (2018)
  • Author(s): 朝比奈靖浩, 伊藤義人, 上野義之, 松崎靖司, 滝川康裕, 八橋弘, 玄田拓哉, 池田房雄, 松田卓磨, Huang K, Massetto Benedetta, Osinusi Anu, Brainard Diana, McHutchison John, 河田則文, 榎本信幸．
  • Organizer: 第54回日本肝臓学会総会
• [Presentation] ヒトiPS細胞由来星細胞様細胞における転写因子LHX2の機能解析． (2018)
  • Author(s): 好正人, 柿沼晴, 金子俊, 紙谷聡英, 佐藤綾子, 角田知之, 四宮恵美, 北畑富貴子, 新田沙由梨, 村川美也子, 井津井康浩, 中川美奈, 東正新, 朝比奈靖浩, 渡辺守．
  • Organizer: 第54回日本肝臓学会総会
• [Presentation] HBs抗原陰性化を目指した治療戦略 B型慢性肝疾患の長期経過におけるウイルス動態およびHBs抗原陰性化予測因子の検討． (2018)
  • Author(s): 村川美也子, 朝比奈靖浩, 渡辺守．
  • Organizer: 第54回日本肝臓学会総会
• [Presentation] DAA治療後の耐性ウイルス出現と肝発癌の完全制御を目指した治療戦略． (2018)
  • Author(s): 中川美奈, 朝比奈靖浩, 渡辺守．
  • Organizer: 第54回日本肝臓学会総会
• [Presentation] HCV NS5Aキメラ株感染培養系を用いたNS5A阻害剤に対する耐性変異と薬剤感受性評価システムの構築． (2018)
  • Author(s): 新田沙由梨, 朝比奈靖浩, 佐藤綾子, 三好正人, 角田知之, 永田紘子, 金子俊, 北畑富貴子, 村川美也子, 中川美奈, 東正新, 柿沼晴, 加藤孝宣, 渡辺守．
  • Organizer: 第54回日本肝臓学会総会
• [Presentation] DAAでC型肝がん診療はどう変わったか? DAAs治療によるHCV排除後の肝発癌抑止効果の検討． (2018)
  • Author(s): 永田紘子, 朝比奈靖浩, 渡辺守．
  • Organizer: 第104回日本消化器病学会総会
• [Presentation] 肝癌の臨床検体を用いた癌関連遺伝子プロファイルと病態の解析． (2018)
  • Author(s): 北畑富貴子, 朝比奈靖浩, 渡辺守．
  • Organizer: 第104回日本消化器病学会総会
• [Presentation] 治療不成功により生じたNS5A変異ウイルスの特性と各種抗ウイルス薬に対する感受性の検討． (2018)
  • Author(s): 新田沙由梨, 朝比奈靖浩, 渡辺守．
  • Organizer: 第104回日本消化器病学会総会
• [Presentation] Twelve Weeks of Ledipasvir/Sofosbuvir All-oral Regimen for Patients with Chronic Hepatitis C Genotype 2 Infection: Integrated Analysis of Three Clinical Studies. (2018)
  • Author(s): Asahina Y, Liu CJ, Gane E, Itoh Y, Kawada N, Ueno Y, Wang CY, Llewellyn J, Osinusi A, Svarovskaia J, Mo H, Crans G, Chuang WL, Chen PJ, Enomoto N.
  • Organizer: 27th conference of the Asian Pacific Association for the Study of the Liver (APASL 2018)
  • Int'l Joint Research
• [Presentation] Host genome mutations and HBV integration in HCC patients with HBV infection. (2018)
  • Author(s): Asahina Y.
  • Organizer: 36th US-Japan Hepatitis Panel Meeting New Approaches to HBV Therapy.
  • Int'l Joint Research / Invited
• [Presentation] Gap between real world data and the clinical trial with DAA for HCV. (2017)
  • Author(s): Asahina Y.
  • Organizer: Taiwan Association for the Study of the Liver 2017
  • Int'l Joint Research / Invited
• [Presentation] Occurrence and recurrence of hepatocellular carcinoma (HCC) in HCV patients treated with direct acting antiviral agents. (2017)
  • Author(s): Asahina Y.
  • Organizer: Korean Association for the Study of the Liver 2017
  • Int'l Joint Research / Invited
• [Presentation] Novel culture model for coinfection of hepatitis B and hepatitis C viruses using human induced pluripotent stem cell-derived hepatic cells for analyses of changes in host-innate immune responses. (2017)
  • Author(s): Kaneko S, Kakinuma S, Asahina Y, Kamiya A, Miyoshi M, Tsunoda T, Inoue-Shinomiya E, Nitta S, Sato A, Asano Y, Nagata H, Kawai-Kitahata F, Murakawa M, Itsui Y, Nakagawa M, Azuma S, Watanabe M.
  • Organizer: The 68th Annual Meeting of the American Association for the Study of Liver Disease (AASLD The Liver Meeting 2017)
  • Int'l Joint Research
• [Presentation] Evaluation of an early occurrence and recurrence of hepatocellular carcinoma in chronic hepatitis C patients treated with DAAs - Retrospective review of a prospective database -. (2017)
  • Author(s): Nakagawa M, Asahina Y, Nagata H, Sato A, Miyoshi M, Tsunoda T, Asano Y, Kaneko S, Kawai-Kitahata F, Murakawa M, Nitta S, Itsui Y, Azuma S, Kakinuma S, Watanabe M.
  • Organizer: The 68th Annual Meeting of the American Association for the Study of Liver Disease (AASLD The Liver Meeting 2017),
  • Int'l Joint Research
• [Presentation] The analysis of NS5A Resistance-Associated Substitutions (RAS): In vitro study of NS5A recombinant hepatitis C in infectious cell culture system for various RAS detected after treatment failure in chronic hepatitis C patients. (2017)
  • Author(s): Nitta S, Murakawa M, Kato T, Sato A, Tsunoda T, Miyoshi M, Asano Y, Kaneko S, Nagata H, Kawai-Kitahata F, Itsui Y, Nakagawa M, Azuma S, Kakinuma S, Asahina Y.
  • Organizer: The 68th Annual Meeting of the American Association for the Study of Liver Disease (AASLD The Liver Meeting 2017)
  • Int'l Joint Research
• [Presentation] On-treatment higher levels of alpha-fetoprotein and M2BPGi are associated with development of hepatocellular carcinoma during nucleos(t)ide analog therapy in patients with HBV chronic infection. (2017)
  • Author(s): Murakawa M, Asahina Y, Nakagawa M, Sato A, Miyoshi M, Tsunoda T, Asano Y, Nagata H, Kaneko S, Kawai-Kitahata F, Nitta S, Itsui Y, Azuma S, Kakinuma S, Watanabe M.
  • Organizer: The 68th Annual Meeting of the American Association for the Study of Liver Disease (AASLD The Liver Meeting 2017)
  • Int'l Joint Research
• [Presentation] Ledipasvir/Sofosbuvir in the Treatment of Japanese Patients with Chronic HCV Genotype 2 Infection. (2017)
  • Author(s): Asahina Y, Itoh Y, Ueno Y, Matsuzaki Y, Takikawa Y, Yatsuhashi H, Genda T, Ikeda F, Matsuda T, Jiang D, Huang KC, Massetto B, Osinusi A, Brainard DM, McHutchison JG, Kawada N, Enomoto N.
  • Organizer: The 68th Annual Meeting of the American Association for the Study of Liver Disease (AASLD The Liver Meeting 2017)
  • Int'l Joint Research
• [Presentation] HCC with or without viral suppression and new predictive biomarkers. (2017)
  • Author(s): Asahina Y.
  • Organizer: Taiwan Digestive Diseases Week 2017
  • Int'l Joint Research / Invited
• [Presentation] Diabetic retinopathy as a risk factor associated with development of hepatocellular carcinoma in non-alcoholic fatty liver disease. (2017)
  • Author(s): Azuma S, Asahina Y, Kakinuma S, Azuma K, Watanabe M.
  • Organizer: Asian Pacific Digestive Week 2017
  • Int'l Joint Research
• [Presentation] Genetic differences in hepatocellular carcinoma among chronic persistent hepatitis B virus (HBV) infection with or without viral suppression and prior HBV infection. (2017)
  • Author(s): Asahina Y, Tanaka S, Kakinuma S, Murakawa M, Nitta S, Watanabe T, Otani S, Goto F, Nagata H, Kaneko S, Azuma S, Itsui Y, Nakagawa M, Tanabe M, Maekawa S, Enomoto N, Watanabe M.
  • Organizer: The 52th annual meeting of the European association for the study of the liver (EASL The International Liver Congress 2017)
  • Int'l Joint Research
• [Presentation] Risk factors for HCC in patients with HCV after anti-viral treatment. (2017)
  • Author(s): Asahina Y.
  • Organizer: The 11th APASL Single Topic Conference
  • Int'l Joint Research / Invited
• [Presentation] SVR100%を目指すDAAs治療 C型慢性肝炎に対するインターフェロンフリー治療不成功および治療後発癌例の検討． (2017)
  • Author(s): 中川美奈, 朝比奈靖浩, 渡辺守．
  • Organizer: 第21回日本肝臓学会大会
• [Presentation] C型慢性肝炎に対するIFN-based、IFN-free治療後SVR後発癌および発癌予測因子についての検討． (2017)
  • Author(s): 永田紘子, 中川美奈, 朝比奈靖浩．
  • Organizer: 第21回日本肝臓会大会
• [Presentation] HBV再活性化対策のup-to-date HBV-HCV共感染時における抗HCV－DAA治療に伴うHBV再活性化機構の解析． (2017)
  • Author(s): 金子俊, 柿沼晴, 朝比奈靖浩．
  • Organizer: 第21回日本肝臓学会大会
• [Presentation] 慢性肝疾患の線維化発がん阻止への展望 ヒトiPS細胞由来肝臓系譜細胞による病態モデルの作成と先天性肝線維症の病態解析. (2017)
  • Author(s): 角田知之, 柿沼 晴, 三好正人, 佐藤綾子, 四宮恵美, 金子 俊, 浅野 侑, 村川美也子, 新田沙由梨, 井津井康浩, 東 正新, 中川美奈, 朝比奈靖浩, 渡辺 守.
  • Organizer: 第54回日本消化器免疫学会総会
• [Presentation] C型肝炎の最新治療と新たなる課題. (2017)
  • Author(s): 朝比奈靖浩.
  • Organizer: 第16回日本ウイルス学会
  • Invited
• [Presentation] 肝炎ウイルス制御下およびB型肝炎ウイルス既往感染におけるにおける肝細胞癌遺伝子変異の検討． (2017)
  • Author(s): 北畑富貴子，朝比奈靖浩，田中真二，村川美也子，新田沙由梨，柿沼晴，永田紘子，金子俊，浅野侑，角田知之，三好正人，井上恵美，佐藤綾子，井津井康浩，中川美奈，東正新，田邉稔，前川伸哉，榎本信幸，渡辺守．
  • Organizer: 第53回日本肝癌研究会
• [Presentation] C型肝炎の最新治療と新たなる課題. (2017)
  • Author(s): 朝比奈靖浩.
  • Organizer: 第30回日本消化器病学会関東支部教育講演
  • Invited
• [Presentation] 日本肝臓学会ガイドラインup to date HCV感染と肝発癌. (2017)
  • Author(s): 朝比奈靖浩.
  • Organizer: 第53回日本肝臓学会総会
  • Invited
• [Presentation] ウイルス制御を目指したB型肝炎の治療戦略 B型慢性肝炎TDFまたはTAF投与例における骨密度の変化． (2017)
  • Author(s): 朝比奈靖浩, 泉並木．
  • Organizer: 第53回日本肝臓学会総会
  • Invited
• [Presentation] B型肝炎研究の新展開 ヒトiPS細胞由来肝細胞系譜細胞を用いたB型肝炎ウイルス感染への宿主自然免疫応答の解析． (2017)
  • Author(s): 金子俊, 柿沼晴, 朝比奈靖浩．
  • Organizer: 第53回日本肝臓学会総会
• [Presentation] C型肝炎SVR後の問題点 C型慢性肝炎に対するIFN-based、IFN-free治療後発癌および発癌予測因子についての検討． (2017)
  • Author(s): 永田紘子, 中川美奈, 朝比奈靖浩．
  • Organizer: 第53回日本肝臓学会総会
• [Presentation] ウイルス性肝炎の病態解析と新規治療 B型肝炎ウイルス制御下における肝細胞癌遺伝子変異の検討． (2017)
  • Author(s): 北畑富貴子, 朝比奈靖浩, 渡辺守．
  • Organizer: 第53回日本肝臓学会総会
• [Presentation] C型肝炎インターフェロンフリー治療の現況 C型慢性肝疾患に対するインターフェロンフリー治療の有効性の検討． (2017)
  • Author(s): 中川美奈, 朝比奈靖浩, 渡辺守．
  • Organizer: 第103回日本消化器病学会大会
• [Book] 臨床検査のガイドライン (2019)
  • Author(s): 朝比奈靖浩.
  • Total Pages: 473
  • Publisher: 宇宙堂八木書店
• [Book] 消化器疾患最新の治療2019-2020 (2019)
  • Author(s): 朝比奈靖浩.
  • Total Pages: 497
  • Publisher: 南江堂
• [Book] 臨床薬理学 (2019)
  • Author(s): 朝比奈靖浩.
  • Total Pages: 331
  • Publisher: 医学書院
• [Book] ウイルス性肝炎 (2019)
  • Author(s): 朝比奈靖浩.
  • Total Pages: 245
  • Publisher: 最新医学社