Is decellularized xenogeneic meniscus an alternative to allogeneic meniscus for meniscus transplantation?

• Project/Area Number: 17K19717
• Research Category: Grant-in-Aid for Challenging Research (Exploratory)
• Allocation Type: Multi-year Fund
• Research Field: Surgery related to the biological and sensory functions and related fields
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: sekiya ichiro 東京医科歯科大学, 統合研究機構, 教授 (10345291)
• Co-Investigator(Kenkyū-buntansha): 岸田 晶夫 東京医科歯科大学, 生体材料工学研究所, 教授 (60224929)
• Project Period (FY): 2017-06-30 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000); Fiscal Year 2019: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2018: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2017: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 移植・再生医療 / 再生医学

Outline of Final Research Achievements

We decellularized menisci using high hydrostatic pressure (HHP) and compared the decellularized menisci with freeze-thawed menisci in vitro and in vivo. Decellularization of meniscus was confirmed by histological and biochemical analyses. The compression strength of the HHP-decellularized menisci slightly decreased compared with freeze-thawed menisci. After implantation, the numbers of immune cells were significantly lower around the HHP-treated menisci than around the freeze-thawed menisci. Recellularization of the HHP-decellularized menisci was confirmed.

Academic Significance and Societal Importance of the Research Achievements

本邦で制限されている同種半月板移植に代わる新たな半月板欠損に対する治療法の構築

Research Products

• [Journal Article] Comparison of High‐Hydrostatic‐Pressure Decellularized Versus Freeze‐Thawed Porcine Menisci (2019)
  • Author(s): Watanabe Naoto、Mizuno Mitsuru、Matsuda Junpei、Nakamura Naoko、Otabe Koji、Katano Hisako、Ozeki Nobutake、Kohno Yuji、Kimura Tsuyoshi、Tsuji Kunikazu、Koga Hideyuki、Kishida Akio、Sekiya Ichiro
  • Journal Title: Journal of Orthopaedic Research Volume: 37 Issue: 11 Pages: 2466-2475
  • DOI: 10.1002/jor.24350
  • Peer Reviewed / Open Access
• [Presentation] The amount of collagen is not related to mechanical properties in high hydrostatic pressure-decellularized menisci and freeze-thawed menisci. (2020)
  • Author(s): Naoto Watanabe, Mitsuru Mizuno, Junpei Matsuda, Naoko Nakamura, Koji Otabe, Hisako Katano, Nobutake Ozeki, Yuji Kohno, Tsuyoshi Kimura, Kunikazu Tsuji, Hideyuki Koga, Akio Kishida, Ichiro Sekiya.
  • Organizer: Orthopaedic Research Society
  • Int'l Joint Research
• [Presentation] 高静水圧印加処理による脱細胞化半月板と凍結融解処理半月板の比較 (2020)
  • Author(s): 渡部直人、水野満、松田純平、大関信武、片野尚子、小田邉浩二、辻邦和、古賀英之、大川淳、関矢一郎.
  • Organizer: 日本整形外科学会学術総会
• [Presentation] 高静水圧印加処理による脱細胞化半月板と凍結融解処理半月板の機械的強度と生体適合性の比較 (2019)
  • Author(s): 渡部直人、水野満、松田純平、大関信武、片野尚子、小田邉浩二、河野佑二、辻邦和、古賀英之、大川淳、関矢一郎.
  • Organizer: 日本整形外科基礎学術集会(シンポジウム）
• [Presentation] 高静水圧印加処理を用いた脱細胞化半月板の作製および力学的強度の評価 (2018)
  • Author(s): 渡部直人、大関信武、小田邉浩二、岸田晶夫、宗田大、古賀英之、関矢一郎
  • Organizer: 日本関節鏡・膝・スポーツ整形外科学会（ポスター）
• [Presentation] 高静水圧印加処理による脱細胞化半月板は凍結融解処理同種半月板移植の代替材料に成りうるか (2018)
  • Author(s): 渡部直人、水野満、中村奈緒子、松田純平、大関信武、片野尚子、木村剛、岸田晶夫、古賀英之、大川淳、関矢一郎
  • Organizer: 日本整形外科基礎学術集会（口演）
• [Presentation] Comparison of decellularized meniscus by high hydrostatic pressure with freeze-thawed meniscus (2018)
  • Author(s): Naoto Watanabe, Mitsuru Mizuno, Junpei Matsuda, Naoko Nakamura, Koji
  • Organizer: Orthopaedic Research Society（国際学会、ポスター）
  • Int'l Joint Research
• [Presentation] 2.Ultra-High Hydrostatic Pressureを用いた脱細胞化半月板の作製および力学的強度の評価 (2017)
  • Author(s): 1.渡部直人、水野満、中村奈緒子、松田純平、片野尚子、大関信武、木村剛、岸田晶夫、辻邦和、宗田大、関矢一郎
  • Organizer: 日本臨床バイオメカニクス学会（口演）
• [Presentation] 高静水圧印加処理による脱細胞化半月板の作製 (2017)
  • Author(s): 1.渡部直人、水野満、中村奈緒子、松田純平、大関信武、片桐健太、河野佑二、串田淑久、片野尚子、木村剛、岸田晶夫、辻邦和、宗田大、大川淳、関矢一郎
  • Organizer: 日本整形外科基礎学術集会（口演）
• [Presentation] 2.Can Decellularized Meniscus by High Hydrostatic Pressure be an Alternative to Meniscus Allograft? - Compared with Deep Frozen Meniscus (2017)
  • Author(s): 1.Naoto Watanabe, Mitsuru Mizuno, Junpei Matsuda, Naoko Nakamura, Koji
  • Organizer: Orthopaedic Research Society（国際学会、口演）
  • Int'l Joint Research