Physiological characteristics of female in terms of polymorphonuclear leukocyte behavior: Are there sex-differences at the cellular level?
| Project/Area Number |
17K19734
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| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Surgery related to the biological and sensory functions and related fields
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| Research Institution | Juntendo University |
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Principal Investigator |
Araki Yoshihiko 順天堂大学, 医学(系)研究科(研究院), 先任准教授 (70250933)
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| Co-Investigator(Kenkyū-buntansha) |
吉武 洋 順天堂大学, 医学(系)研究科(研究院), 非常勤講師 (00396574)
藤原 浩 金沢大学, 医学系, 教授 (30252456)
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| Project Period (FY) |
2017-06-30 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2018: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2017: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
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| Keywords | 多形核白血球 / RP-3 / ラット / 妊娠 / 出産 / 性差 / 多形核白血球枯渇化抗体 / 単クローン抗体 / 胎盤 |
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| Outline of Final Research Achievements |
Polymorphonuclear leukocytes (PMNs) are effectors involved in parturition through immunoregulatory networks. Although PMNs consist of a substantial population of leukocytes, sex-differences remain unclear. Here, we demonstrate cellular diversity of PMNs in rats monitored using a specific monoclonal antibody, RP-3. Cell-sorting analyses showed strong reactivity to RP-3 (RP-3high) in the majority of male-PMNs, whereas female-PMNs included an additional population of cells showing low reactivity (RP-3low). In pregnant, cyclical changes between these two-group were observed during the gestational-day (GD). Especially, the majority of PMNs were RP-3high at GD-19, followed by a strong increase in RP-3low at GD-20, with the distinction maintained until before partum. In addition, a subtype having almost no reactivity to RP-3 on the PMN surface (RP-3surface null) was observed after RP-3 intraperitoneal injection. These results suggest that there may be three PMN types in female rats.
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| Academic Significance and Societal Importance of the Research Achievements |
多形核白血球（PMN）は免疫調節ネットワークを介して分娩に関与するエフェクターであると考えられている。本研究で示されたPMNの多様性はこれまで知られていなかったPMNの機能解明に大きな研究上の基礎となる重要な学術的発見である。
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Report
(4 results)
Research Products
(18 results)
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[Journal Article] Quantitative measurement of a candidate serum biomarker peptide derived from α2-HS-glycoprotein, and a preliminary trial of multidimensional peptide analysis in females with pregnancy-induced hypertension.2018
Author(s)
Hamamura K, Yanagida M, Ishikawa H, Banzai M, Yoshitake H, Nonaka D, Tanaka K, Sakuraba M, Miyakuni Y, Takamori K, Nojima M, Yoshida K, Fujiwara H, Takeda S, Araki Y.
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Journal Title
Ann Clin Biochem
Volume: 55
Issue: 2
Pages: 287-295
DOI
Related Report
Peer Reviewed
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[Journal Article] Factors regulating human extravillous trophoblast invasion: Chemokine-peptidase and CD9-integrin systems.2018
Author(s)
Fujiwara H, Matsumoto H, Sato Y, Horie A, Ono M, Nakamura M, Mizumoto Y, Kagami K, Fujiwara T, Hattori A, Maida Y, Daikoku T, Imakawa K, Araki Y.
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Journal Title
Current Pharmaceutical Biotechnology
Volume: 19
Issue: 10
Pages: 764-770
DOI
Related Report
Peer Reviewed
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[Journal Article] Platelet-derived microparticles and soluble factors differentially regulate human endometrial epithelial cell movement2017
Author(s)
Suginami K, Sato Y, Horie A, Matsumoto H, Tani H, Mizumoto Y, Ono M, Matsuoka A, Kyo S, Araki Y, Konishi I, Fujiwara H
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Journal Title
Am J Reprod Immunol
Volume: Feb 7
Issue: 4
Pages: 12641-12641
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Presentation] Bisecting GlcNAc, the molecular epitope of an anti-sperm auto-antibody, Ts4 : Its morphological characteristics and identification of the candidate glycoprotein(s) possessing the sugar moiety in mice.2018
Author(s)
Oda R, Yoshitake H, Kazuno S, Miura Y, Ueno T, Sakuraba M, Takamori K, Hasegawa A, Fujiwara H, Takeda S, Araki Y.
Organizer
Society for the Study of Reproduction, 51st Annual Meeting
Related Report
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