Identification of novel osteoclast-osteoblast coupling factors in exosomes involving cell cycle machinery

• Project/Area Number: 17K19746
• Research Category: Grant-in-Aid for Challenging Research (Exploratory)
• Allocation Type: Multi-year Fund
• Research Field: Oral Science and related fields
• Research Institution: The University of Tokyo
• Principal Investigator: Ogasawara Toru 東京大学, 保健・健康推進本部, 講師 (20359623)
• Co-Investigator(Kenkyū-buntansha): 筑田 博隆 群馬大学, 大学院医学系研究科, 教授 (30345219) ; 茂呂 徹 東京大学, 医学部附属病院, 特任教授 (20302698) ; 阿部 雅修 東京大学, 医学部附属病院, 講師 (10392333)
• Project Period (FY): 2017-06-30 – 2023-03-31
• Project Status: Completed (Fiscal Year 2022)
• Budget Amount: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000); Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2018: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: シグナル伝達 / 再生医学 / 発生・分化 / 細胞周期 / エクソソーム

Outline of Final Research Achievements

In the present study, we investigated the existence of an exosome-mediated signaling mechanism between osteoclasts and osteoblasts and its involvement in cell cycle regulation and cell differentiation. However, we could not clearly demonstrate the existence of an exosome-mediated signaling mechanism between osteoblasts and osteoclasts. Therefore, we searched for candidate microRNAs in mouse osteoblast-derived exosomes using small RNA sequencing. We identified mmu-miR-26a-5p as a microRNA that may be involved in the inhibition of osteogenesis, and mmu-miR-214-3p as a microRNA that may be involved in the promotion of osteogenesis.

Academic Significance and Societal Importance of the Research Achievements

本研究によって、骨形成抑制に関わる可能性を持つマイクロRNAとしては、mmu-miR-26a-5pなどの2分子を、骨形成促進に関わる可能性を持つマイクロRNAとしては、mmu-miR-214-3pなどの3分子を同定することが出来た。本研究の成果は、将来的には新たな骨再生医療法開発につながる可能性がある。

Research Products

• [Journal Article] Application of Dental Pulp Stem Cells for Bone and Neural Tissue Regeneration in Oral and Maxillofacial Region. (2023)
  • Author(s): Fujii Y, Hatori A, Chikazu D, Ogasawara T.
  • Journal Title: Stem Cells Int. Volume: 2023 Pages: 2026572-2026572
  • DOI: 10.1155/2023/2026572
  • Peer Reviewed / Open Access
• [Journal Article] Application of Stem Cells in the Oral and Maxillofacial Region (2020)
  • Author(s): Ogasawara Toru、Ko Edward Chengchuan、Yu Jiashing
  • Journal Title: Stem Cells International Volume: 2020 Pages: 1-2
  • DOI: 10.1155/2020/2421453
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] Highly efficient differentiation of chondrocytes from human dental pulp stem cells using a thienoindazole derivative small compound TD-198946. (2022)
  • Author(s): Kanno Y, Ogasawara T et al.
  • Organizer: ASBMR 2022 Annual Meeting
  • Int'l Joint Research
• [Presentation] VCAM-1 and GFPT-2: Predictive markers of osteoblast differentiation in human dental pulp stem cells. (2022)
  • Author(s): Hatori A, Ogasawara T et al.
  • Organizer: ASBMR 2022 Annual Meeting
  • Int'l Joint Research
• [Presentation] ホメオボックス転写因子Nanogは間葉系細胞の骨軟骨分化において重要である (2020)
  • Author(s): 小笠原 徹
  • Organizer: 第19回日本再生医療学会総会
• [Remarks] 東京大学医学部口腔顎顔面外科学教室ホームページ
  • URL: http://plaza.umin.ac.jp/~oralsurg/
• [Remarks] 東京大学医学部附属病院 口腔顎顔面外科・矯正歯科ホームページ
  • URL: http://plaza.umin.ac.jp/~oralsurg/
• [Remarks] 東京大学医学部附属病院口腔顎顔面外科・矯正歯科ホームページ
  • URL: http://plaza.umin.ac.jp/~oralsurg/