Nano-scale observation of differentiating periodontal ligament cells

Research Project

Project/Area Number	17K19750
Research Category	Grant-in-Aid for Challenging Research (Exploratory)
Allocation Type	Multi-year Fund
Research Field	Oral Science and related fields
Research Institution	Osaka University
Principal Investigator	Murakami Shinya 大阪大学, 歯学研究科, 教授 (70239490)
Co-Investigator(Kenkyū-buntansha)	竹立匡秀大阪大学, 歯学部附属病院, 講師 (60452447) 岩山智明大阪大学, 歯学研究科, 助教 (80757865)
Research Collaborator	OGURA Toshihiko OKADA Tomoko OGURA Taku Fratzl Peter
Project Period (FY)	2017-06-30 – 2019-03-31
Project Status	Completed (Fiscal Year 2018)
Budget Amount *help	¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000) Fiscal Year 2018: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000) Fiscal Year 2017: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Keywords	骨芽細胞 / 基質小胞 / 走査電子誘電率顕微鏡 / ナノレベル / リソソーム / 誘電率顕微鏡 / ナノレベル観察 / 歯学
Outline of Final Research Achievements	Mineralization is predominantly mediated by osteoblasts, which secrete mineral precursors, most likely through matrix vesicles (MVs). Here, we utilize field emission-scanning electron microscopy-based scanning electron-assisted microscopy for assessing live osteoblasts in mineralizing conditions at a nano-level resolution. We found that the calcium-containing vesicles were multivesicular bodies containing MVs. They were transported via lysosome and secreted by exocytosis.
Academic Significance and Societal Importance of the Research Achievements	本研究成果により、骨や歯といった硬組織形成の基本的なメカニズムに関する理解が深まり、骨粗鬆症や歯周病等の硬組織疾患の病態解明や治療法の開発につながることが期待されます。さらに、今回観察に用いた誘電率顕微鏡は培養細胞のみならず、様々な微小物質を溶液中で直接観察することが可能であり、今後も様々な分野での応用が期待されます。