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in toto imaging of periodontal ligament-bone marrow complex

Research Project

Project/Area Number 17K19752
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Research Field Oral Science and related fields
Research InstitutionOsaka University

Principal Investigator

Iwayama Tomoaki  大阪大学, 歯学研究科, 助教 (80757865)

Co-Investigator(Kenkyū-buntansha) 野崎 一徳  大阪大学, 歯学部附属病院, 助教 (40379110)
村上 伸也  大阪大学, 歯学研究科, 教授 (70239490)
Project Period (FY) 2017-06-30 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2018: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2017: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Keywords歯根膜 / 組織透明化 / ライトシート顕微鏡 / ナノCT / 超解像顕微鏡 / レポーターマウス / ペリサイト / 標準化 / セグメンテーション / 歯学
Outline of Final Research Achievements

We utilized a variety of fluorescent reporter mice and fluorescent tags with tissue clearing techniques to visualize murine periodontal tissue. Maxilla tissue was successfully cleared and observed into the depth of up to 1 mm. in toto imaging revealed that non-collagen producing mesenchymal cells in periodontal ligament was located adjacent to capillaries. As an alternative, nano-CT could be used for in toto imaging without tissue clearing. We also found the basic mechanism of mineralization using super-resolution microscopy.

Academic Significance and Societal Importance of the Research Achievements

生体組織の機能を詳細に理解するためには、脈管・神経のネットワークに代表されるような組織内の細胞どうしの連携や相互作用を連続性を持って観察することが求められる。本研究成果は、歯周組織全体を俯瞰して理解するための基礎研究であり、組織透明化技術と細胞ラベリング技術を応用することにより、マウス歯周組織内の様々な細胞群の網羅的かつ迅速な検出を実現した。一連の解析手法はヒトや他臓器にも応用可能であり、基礎医学分野への技術移転も期待される。

Report

(3 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • Research Products

    (3 results)

All 2018

All Presentation (3 results) (of which Int'l Joint Research: 1 results)

  • [Presentation] 初代培養マウス歯根膜由来細胞のクローン解析2018

    • Author(s)
      上田亜美、岩山智明、冨田貴和子、平井麻絵、竹立匡秀、村上伸也
    • Organizer
      第61回春季日本歯周病学会学術大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 前骨芽細胞コミットメント調節因子の探索2018

    • Author(s)
      岩山智明
    • Organizer
      第3回Skeletal Science Retreat
    • Related Report
      2018 Annual Research Report
  • [Presentation] Clonal analysis of primary murine periodontal ligament-derived cells2018

    • Author(s)
      Tsugumi Ueda, Tomoaki Iwayama, Kiwako Tomita, Asae Hirai, Masahide Takedachi, Shinya Murakami
    • Organizer
      2018 IADR GENERAL SESSION
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research

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Published: 2017-07-21   Modified: 2020-03-30  

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