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Study on the generation of oral cancer stem cell and analysis of its nature

Research Project

Project/Area Number 17K19760
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Research Field Oral Science and related fields
Research InstitutionThe University of Tokushima

Principal Investigator

KUDO Yasusei  徳島大学, 大学院医歯薬学研究部(歯学域), 准教授 (50314753)

Co-Investigator(Kenkyū-buntansha) 石丸 直澄  徳島大学, 大学院医歯薬学研究部(歯学域), 教授 (60314879)
常松 貴明  徳島大学, 大学院医歯薬学研究部(医学域), 助教 (70726752)
Project Period (FY) 2017-06-30 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2018: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2017: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Keywords口腔癌 / 癌幹細胞 / 細胞周期 / 口腔癌幹細胞 / Emi1 / 幹細胞 / ユビキチン分解
Outline of Final Research Achievements

Cancer stem cells cause relapse by treatment resistance. Therefore, removal of cancer stem cells is considered to be a useful therapeutic method for preventing metastasis and recurrence. So far, much, the nature and its presence of oral cancer stem cells have not been clarified. In the present study, artificial oral cancer stem cells (iOCS) were generated via constitutively inactivating APC/C by overexpression of Emi1 in oral cancer cells to mimick the cell cycle control of embryonic stem cells. We found that the iOCS cells had stem cell properties such as reduced proliferation, shortened G1 phase and longer S phase in the cell cycle, and increased expression of stem cell markers.

Academic Significance and Societal Importance of the Research Achievements

近年、自己複製能や多分化能を有する癌幹細胞の存在が明らかとなり、癌幹細胞を起源として癌が発生すると考えられている。実際に、白血病や脳腫瘍、大腸癌、乳癌などの癌で幹細胞が発見されているが、口腔癌幹細胞に関する研究は他臓器の癌に比べて遅れており、未だその存在や性質は明らかにされていない。本研究で作製した「人工口腔癌幹細胞」の性状を詳細に検討することにより、将来の口腔癌幹細胞に関する研究基盤になることが期待される。さらに、本研究成果は口腔癌における新規の診断マーカーや抗がん剤の開発につながることが期待される。

Report

(3 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • Research Products

    (7 results)

All 2019 2018 Other

All Int'l Joint Research (2 results) Journal Article (3 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 3 results,  Open Access: 2 results) Presentation (2 results) (of which Invited: 1 results)

  • [Int'l Joint Research] New York University(米国)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] ニューヨーク大学医学部・ハワードヒューズ医学研究所(米国)

    • Related Report
      2017 Research-status Report
  • [Journal Article] Predicting cancer outcome: Artificial intelligence vs. pathologists2019

    • Author(s)
      Kudo Yasusei
    • Journal Title

      Oral Diseases

      Volume: 25 Issue: 3 Pages: 643-645

    • DOI

      10.1111/odi.12954

    • NAID

      120006884847

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Aurora Kinase Inhibitors In Head And Neck Cancer.2018

    • Author(s)
      Qi G, Liu J, Mi S, Tsunematsu T, Jin S, Shao W, Liu T, Ishimaru N, Tang B, Kudo Y.
    • Journal Title

      Curr Top Med Chem.

      Volume: - Issue: 3 Pages: 199-213

    • DOI

      10.2174/1568026618666180112163741

    • NAID

      120006729373

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Invasion-related factors as potential diagnostic and therapeutic targets in oral squamous cell carcinoma.2018

    • Author(s)
      Siriwardena SBSM, Tsunematsu T, Qi G, Ishimaru N, Kudo Y.
    • Journal Title

      Int J Mol Sci

      Volume: 19 Issue: 5 Pages: 1462-1462

    • DOI

      10.3390/ijms19051462

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Emi1の過剰発現による人工口腔癌幹細胞の作成2018

    • Author(s)
      西條 早紀, 大塚 邦紘, 常松 貴明, 大塚 邦紘, 牛尾 綾, 新垣 理恵子, 工藤 保誠, 石丸 直澄
    • Organizer
      第107回日本病理学会総会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 細胞周期調節を標的としたがん幹細胞の分化誘導とその臨床応用の可能性2018

    • Author(s)
      工藤保誠
    • Organizer
      2018先端医学研究交流セミナー「がん・白血病ー先端研究の現状ー」
    • Related Report
      2018 Annual Research Report
    • Invited

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Published: 2017-07-21   Modified: 2022-02-21  

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