Development of bloodstain identification by using Raman spectroscopy
| Project/Area Number |
17K19814
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| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Society medicine, Nursing, and related fields
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| Research Institution | Shimane University |
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Principal Investigator |
Fujihara Junko 島根大学, 学術研究院医学・看護学系, 助教 (20346381)
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| Project Period (FY) |
2017-06-30 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2019: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2017: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
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| Keywords | ラマン分光法 / 非破壊検査 / 血痕検査 / 法医鑑識科学 / 血痕 / ヘモグロビン / ポータブルラマン / 新規検査法 |
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| Outline of Final Research Achievements |
In the present study, blood identification and discrimination between human and nonhuman blood were performed by portable Raman spectrometer. Raman spectra of bloodstains from 11 species were taken using a portable Raman spectrometer. Raman peaks for blood (742, 1001, 1123, 1247, 1341, 1368, 1446, 1576, and 1619 cm-1) could be observed by the in all 11 species, and the human bloodstain could be distinguished from the nonhuman ones by using a principal component analysis.
In addition, we used micro-Raman spectroscopy with high-resolution analysis to discriminate between bloodstains from infants and bloodstains from adults. A peak was obtained at 1105 cm－1, which was assigned to histidine; this peak was observed only for bloodstains from adults. This study discriminate between bloodstains from infants and bloodstains from adults using micro-Raman spectroscopy, with beneficial implications in forensic science.
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| Academic Significance and Societal Importance of the Research Achievements |
血痕検査は犯最も重要な法医学的物体検査である。血痕検査の主目的は個人識別（DNA鑑定）にある。血痕予備試験にはロイコマラカイトグリーン法、ルミノール法が用いられるが、これらには①非特異的反応がみられる②試薬の調整が必要である③試薬によりDNAが分解されてしまう等の難点がある。最大の難点は、微量な試料の場合予備試験を行うとその後のDNA鑑定ができない点である。ラマン分光法は、非破壊、非接触で分析することができ、この難点の克服が可能である。また、ポータブルラマン分光器が普及し始め、屋外などでの測定も可能となっており、DNA検査前のスクリーニング検査法として法医鑑識活動に非常に有用である。
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Report
(5 results)
Research Products
(44 results)
