• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development of bloodstain identification by using Raman spectroscopy

Research Project

Project/Area Number 17K19814
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Research Field Society medicine, Nursing, and related fields
Research InstitutionShimane University

Principal Investigator

Fujihara Junko  島根大学, 学術研究院医学・看護学系, 助教 (20346381)

Project Period (FY) 2017-06-30 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2019: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2017: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Keywordsラマン分光法 / 非破壊検査 / 血痕検査 / 法医鑑識科学 / 血痕 / ヘモグロビン / ポータブルラマン / 新規検査法
Outline of Final Research Achievements

In the present study, blood identification and discrimination between human and nonhuman blood were performed by portable Raman spectrometer. Raman spectra of bloodstains from 11 species were taken using a portable Raman spectrometer. Raman peaks for blood (742, 1001, 1123, 1247, 1341, 1368, 1446, 1576, and 1619 cm-1) could be observed by the in all 11 species, and the human bloodstain could be distinguished from the nonhuman ones by using a principal component analysis.
In addition, we used micro-Raman spectroscopy with high-resolution analysis to discriminate between bloodstains from infants and bloodstains from adults. A peak was obtained at 1105 cm-1, which was assigned to histidine; this peak was observed only for bloodstains from adults. This study discriminate between bloodstains from infants and bloodstains from adults using micro-Raman spectroscopy, with beneficial implications in forensic science.

Academic Significance and Societal Importance of the Research Achievements

血痕検査は犯最も重要な法医学的物体検査である。血痕検査の主目的は個人識別(DNA鑑定)にある。血痕予備試験にはロイコマラカイトグリーン法、ルミノール法が用いられるが、これらには①非特異的反応がみられる②試薬の調整が必要である③試薬によりDNAが分解されてしまう等の難点がある。最大の難点は、微量な試料の場合予備試験を行うとその後のDNA鑑定ができない点である。ラマン分光法は、非破壊、非接触で分析することができ、この難点の克服が可能である。また、ポータブルラマン分光器が普及し始め、屋外などでの測定も可能となっており、DNA検査前のスクリーニング検査法として法医鑑識活動に非常に有用である。

Report

(5 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (44 results)

All 2021 2020 2019 2018 2017 2016

All Journal Article (21 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 20 results,  Open Access: 6 results,  Acknowledgement Compliant: 3 results) Presentation (23 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] Dermal absorption of gallium antimonide in vitro and pro-inflammatory effects on human dermal fibroblasts2021

    • Author(s)
      Fujihara Junko、Nishimoto Naoki
    • Journal Title

      Toxicology in Vitro

      Volume: 71 Pages: 105064-105064

    • DOI

      10.1016/j.tiv.2020.105064

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The indicators associated with increasing suicide trends: Need for harmony in discussing suicide in legal medicine and other fields2021

    • Author(s)
      Inoue Ken、Apbassova Madina、Hoshi Masaharu、Takeichi Nobuo、Noso Yoshihiro、Ohira Yoshiyuki、Shabdarbayeva Dariya、Chaizhunusova Nailya、Zhunussov Yersin T.、Fujihara Junko、Kimura-Kataoka Kaori、Fujita Yasuyuki、Takeshita Haruo
    • Journal Title

      Legal Medicine

      Volume: 50 Pages: 101820-101820

    • DOI

      10.1016/j.legalmed.2020.101820

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Total antimony analysis by hydride generation-microwave plasma-atomic emission spectroscopy with applications2020

    • Author(s)
      Fujihara Junko、Nishimoto Naoki
    • Journal Title

      Microchemical Journal

      Volume: 157 Pages: 104992-104992

    • DOI

      10.1016/j.microc.2020.104992

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of GaSb thin films with excess Ga grown by RF magnetron sputtering2020

    • Author(s)
      Nishimoto Naoki、Fujihara Junko
    • Journal Title

      International Journal of Modern Physics B

      Volume: 34 Issue: 10 Pages: 2050097-2050097

    • DOI

      10.1142/s0217979220500976

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Association of SNPs in transferrin and transferrin receptor genes with blood iron levels in human2019

    • Author(s)
      Fujihara Junko、Yasuda Toshihiro、Kimura-Kataoka Kaori、Takeshita Haruo
    • Journal Title

      Legal Medicine

      Volume: 36 Pages: 17-20

    • DOI

      10.1016/j.legalmed.2018.09.022

    • Related Report
      2019 Research-status Report 2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Circulating cell-free DNA fragment analysis by microchip electrophoresis and its relationship with DNase I in cardiac diseases2019

    • Author(s)
      Fujihara J, Takinami Y, Ueki M, Kimura-Kataoka K, Yasuda T, Takeshita H
    • Journal Title

      Clinica Chimica Acta

      Volume: 497 Pages: 61-66

    • DOI

      10.1016/j.cca.2019.07.014

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] Analysis of copy number variation in the NEDD4L gene potentially implicated in body height in the Japanese population2019

    • Author(s)
      Ueki Misuzu、Takeshita Haruo、Fujihara Junko、Kimura-Kataoka Kaori、Iida Reiko、Yasuda Toshihiro
    • Journal Title

      Legal Medicine

      Volume: 37 Pages: 83-85

    • DOI

      10.1016/j.legalmed.2019.02.003

    • Related Report
      2019 Research-status Report 2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Low genetic heterogeneity of copy number variations (CNVs) in the genes encoding the human deoxyribonucleases 1-like 3 and II potentially relevant to autoimmunity.2019

    • Author(s)
      M. Ueki, H. Takeshita, J. Fujihara, K. Kimura, K. Yamada, Y. Takinami, R. Iida, T. Yasuda.
    • Journal Title

      PLOS ONE

      Volume: 14(4) Issue: 4 Pages: 214579-214588

    • DOI

      10.1371/journal.pone.0215479

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Evaluation of the functional effects of genetic variants?missense and nonsense SNPs, indels and copy number variations?in the gene encoding human deoxyribonuclease I potentially implicated in autoimmunity2019

    • Author(s)
      Ueki M, Kimura-Kataoka K, Fujihara J, Iida R, Kawai Y, Kusaka A, Sasaki T, Takeshita H, Yasuda T.
    • Journal Title

      Scientific Reports

      Volume: 9 Issue: 1 Pages: 13660-13660

    • DOI

      10.1038/s41598-019-49935-y

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Characterization of a Flexible InGaSb/PI Thin Film Grown by RF Magnetron Sputtering and Aqueous Stability Improvement via Surface Coating2019

    • Author(s)
      Nishimoto N and Fujihara J.
    • Journal Title

      physica status solidi (a)

      Volume: 216 Issue: 4 Pages: 1800860-1800860

    • DOI

      10.1002/pssa.201800860

    • Related Report
      2019 Research-status Report 2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Physicochemical and biocompatibility analyses of surface-coated IN0.57SB0.43 thin films under aqueous conditions2019

    • Author(s)
      Nishimoto N and Fujihara J.
    • Journal Title

      International Journal of Modern Physics B

      Volume: 33 Issue: 12 Pages: 1950109-1950109

    • DOI

      10.1142/s0217979219501091

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] Discrimination Between Infant and Adult Bloodstains Using Micro-Raman Spectroscopy: A Preliminary Study2018

    • Author(s)
      Fujihara Junko、Nishimoto Naoki、Yasuda Toshihiro、Takeshita Haruo
    • Journal Title

      Journal of Forensic Sciences

      Volume: 1 Issue: 3 Pages: 1-4

    • DOI

      10.1111/1556-4029.13904

    • Related Report
      2019 Research-status Report 2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Association of SNPs in genes encoding zinc transporters on blood zinc levels in humans2018

    • Author(s)
      Fujihara Junko、Yasuda Toshihiro、Kimura-Kataoka Kaori、Takinami Yoshikazu、Nagao Masataka、Takeshita Haruo
    • Journal Title

      Legal Med

      Volume: 30 Pages: 28-33

    • DOI

      10.1016/j.legalmed.2017.10.009

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] COMPARISON OF THE IN VITRO CYTOTOXICITIES OF NITROGEN DOPED (p-TYPE) AND n-TYPE ZINC OXIDE NANOPARTICLES2018

    • Author(s)
      FUJIHARA JUNKO、HASHIMOTO HIDEKI、NISHIMOTO NAOKI、TONGU MIKI、FUJITA YASUHISA
    • Journal Title

      Surface Review and Letters

      Volume: 25 Issue: 04 Pages: 1850084-1850084

    • DOI

      10.1142/s0218625x18500841

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Biocompatibility of GaSb thin films grown by RF magnetron sputtering.2017

    • Author(s)
      Fujihara J, Nishimoto N, Yoshino K.
    • Journal Title

      Applied surface science

      Volume: 409 Pages: 375-380

    • DOI

      10.1016/j.apsusc.2017.03.099

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Control of the conduction mechanism via the formation of native defects in RF-magnetron-sputtered GaSb thin films on Ge(100) substrates2017

    • Author(s)
      Nishimoto Naoki, Fujihara Junko, Yoshino Katsumi
    • Journal Title

      Journal of Crystal Growth

      Volume: 468 Pages: 732-736

    • DOI

      10.1016/j.jcrysgro.2016.11.082

    • Related Report
      2017 Research-status Report
  • [Journal Article] Survey of single-nucleotide polymorphisms in the gene encoding human deoxyribonuclease I-like 2 producing loss of function potentially implicated in the pathogenesis of parakeratosis2017

    • Author(s)
      Ueki Misuzu、Takeshita Haruo、Utsunomiya Natsuko、Chino Takanao、Oyama Noritaka、Hasegawa Minoru、Kimura-Kataoka Kaori、Fujihara Junko、Iida Reiko、Yasuda Toshihiro
    • Journal Title

      PLOS ONE

      Volume: 12 Issue: 4 Pages: e0175083-e0175083

    • DOI

      10.1371/journal.pone.0175083

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Simple screening method for copy number variations associated with physical features2017

    • Author(s)
      M Ueki, H Takeshita, J Fujihara, K Kimura-Kataoka, R Iida, T Yasuda
    • Journal Title

      Legal Med

      Volume: 25 Pages: 71-74

    • DOI

      10.1016/j.legalmed.2017.01.006

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Two fatal cases of caffeine poisoning and a review of the literature.2017

    • Author(s)
      Fujihara J, Yasuda T. Kimura-Kataoka K, Hasegawa H, Kurata S, Takeshita H.
    • Journal Title

      Shimane J Med Sci

      Volume: 34 Pages: 55-60

    • NAID

      120006375997

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Sequence analysis of ABO and its homologues is valid for species identification.2017

    • Author(s)
      Sano R, Fukuda H, Takahashi Y, Takahashi K, Kubo R, Kobayashi M, Fujihara J, Takeshita H, Kominato Y.
    • Journal Title

      Transfus Med

      Volume: 27 Issue: 6 Pages: 428-436

    • DOI

      10.1111/tme.12455

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Blood identification and discrimination of human and nonhuman using portable Raman spectroscopy2016

    • Author(s)
      Junko Fujihara, Yasuhisa Fujita, Tatsuyuki Yamamoto, Naoki Nishimoto, Kaori Kimura-Kataoka, Satsuki Kurata, Yoshikazu Takinami, Toshihiro Yasuda, Haruo Takeshita
    • Journal Title

      International Journal of Legal Medicine

      Volume: - Issue: 2 Pages: 319-322

    • DOI

      10.1007/s00414-016-1396-2

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] リドカイン代謝に関与するCYP1A2およびCYP3A4遺伝子におけるFunctional SNPsのベトナム人における頻度分布2019

    • Author(s)
      藤原 純子、Yuying Xue、竹下 治男
    • Organizer
      日本法中毒学会第38年会
    • Related Report
      2019 Research-status Report
  • [Presentation] 顕微ラマン分光法を用いた成人と乳児血痕の識別2019

    • Author(s)
      藤原純子、木村かおり、竹下治男、安田年博
    • Organizer
      日本DNA多型学会第28回学術集会
    • Related Report
      2019 Research-status Report
  • [Presentation] 年齢依存性発現分子Mpv17-like proteinのミトコンドリアDNA維持における役割2019

    • Author(s)
      飯田礼子、植木美鈴、竹下治男、藤原純子、木村かおり、安田年博
    • Organizer
      日本DNA多型学会第28回学術集会
    • Related Report
      2019 Research-status Report
  • [Presentation] ハイヅカ湖陸封アユの生活史を通した生態調査2019

    • Author(s)
      田中智美、堀之内正博、藤原純子、吉岡秀和、伊藤康宏、荒西太士
    • Organizer
      平成31年度日本水産学会春季大会
    • Related Report
      2019 Research-status Report
  • [Presentation] ハイヅカ湖陸封アユの生活史を通した生態調査2019

    • Author(s)
      田中智美・堀之内正博・藤原純子・吉岡秀和・伊藤康宏・荒西太士
    • Organizer
      水産学会2019年春季大会
    • Related Report
      2018 Research-status Report
  • [Presentation] Comparison of the in vitro cytotoxicities of nitrogen doped (p-type) and n-type zinc oxide nanoparticles.2018

    • Author(s)
      Fujihara J, Hashimoto H, Nishimoto N, Fujita Y.
    • Organizer
      6th Japan-China Symposium on Nanomedicine.
    • Related Report
      2018 Research-status Report
  • [Presentation] Discrimination between adults and infant bloodstains using Raman spectroscopy.2018

    • Author(s)
      Fujihara J, Nishimoto N, Fujita Y,Yamamoto T, Kimura-Kataoka K, Yasuda T, Takeshita H
    • Organizer
      IALM 2018
    • Related Report
      2018 Research-status Report
  • [Presentation] Survey of copy number variations (CNVs) associated with body height: development of simple screening method for CNVs.2018

    • Author(s)
      Yasuda T, Iida R, Ueki M, Kimura-Kataoka K, Fujihara J, Kazuo Yamada, Takeshita H
    • Organizer
      IALM 2018
    • Related Report
      2018 Research-status Report
  • [Presentation] 心疾患患者の血清中cell-free DNA濃度とDNase I活性の関連.2018

    • Author(s)
      藤原純子、瀧波慶和、竹下治男、木村かおり、植木美鈴、飯田礼子、安田年博
    • Organizer
      日本DNA多型学会第27回学術集会
    • Related Report
      2018 Research-status Report
  • [Presentation] 死後血清中cell-free DNAに関する予備的検討2018

    • Author(s)
      藤原純子、竹下治男、木村かおり、飯田礼子、安田年博
    • Organizer
      日本DNA多型学会第27回学術集会
    • Related Report
      2018 Research-status Report
  • [Presentation] DNase familyの遺伝子多型および酵素活性解析2018

    • Author(s)
      木村かおり、藤原純子、山田和夫、植木美鈴、飯田礼子、安田年博、竹下治男
    • Organizer
      日本DNA多型学会第27回学術集会
    • Related Report
      2018 Research-status Report
  • [Presentation] A3, B3型におけるABO遺伝子プロモーターの一塩基置換はCTCFの結合を減弱させる.2018

    • Author(s)
      佐野利恵, 高橋遥一郎, 窪理英子, 早川輝, 藤原純子, 竹下治男, 小湊慶彦.
    • Organizer
      日本DNA多型学会第27回学術集会
    • Related Report
      2018 Research-status Report
  • [Presentation] 複数地域由来Mycobacterium avium株におけるVNTR遺伝子型と薬剤感受性の関係性についての検討.2018

    • Author(s)
      多田納豊. 戸村雪花. 佐野千晶, 梅田啓, 御手洗聡, 吉田志緒美, 露口一成, 藤原純子, 竹下治男, 八木秀樹, 富岡治明.
    • Organizer
      第91回日本細菌学会総会.
    • Related Report
      2018 Research-status Report
  • [Presentation] 血中ZnおよびFe濃度に関与する遺伝子多型部位の予備的探索2017

    • Author(s)
      藤原純子、木村かおり、竹下治男、倉田さつき、安田年博
    • Organizer
      第101次 日本法医学会総会・学術集会
    • Related Report
      2017 Research-status Report
  • [Presentation] GaSb薄膜の毒性評価2017

    • Author(s)
      藤原純子、西本尚己、安田年博、長谷川正紀、竹下治男
    • Organizer
      日本法中毒学会第36 年会
    • Related Report
      2017 Research-status Report
  • [Presentation] 心疾患患者における血清中cell-free DNAに関する予備的検討2017

    • Author(s)
      藤原純子、瀧波慶和、竹下治男、木村かおり、安田年博、倉田さつき
    • Organizer
      日本DNA多型学会第26回学術集会
    • Related Report
      2017 Research-status Report
  • [Presentation] ABO式血液型遺伝子とのパラログ、オーソログの遺伝子解析による種判別法2017

    • Author(s)
      佐野理恵、福田治紀、高橋遥一郎、窪理恵子、高橋圭子、藤原純子、竹下治男、小湊慶彦
    • Organizer
      第101次 日本法医学会総会・学術集会
    • Related Report
      2017 Research-status Report
  • [Presentation] 自己免疫疾患に関与するヒトDNase family遺伝子のfunctionalSNPs2017

    • Author(s)
      安田年博、飯田礼子、植木美鈴、竹下治男、藤原純子、木村かおり、山田和夫、河合康幸
    • Organizer
      第101次 日本法医学会総会・学術集会
    • Related Report
      2017 Research-status Report
  • [Presentation] ヒト肝細胞におけるAgナノ粒子の細胞毒性およびアポトーシス経路2017

    • Author(s)
      薛玉英、王君君、高暁潔、藤原純子、竹下治男
    • Organizer
      日本法中毒学会第36 年会
    • Related Report
      2017 Research-status Report
  • [Presentation] 国内外の多集団におけるInterleukin 8遺伝子 (CXCL8) 内-251 A/T 多型解析においてみられた地理的勾配および喫煙率との相関2017

    • Author(s)
      竹下治男、藤原純子、木村かおり、山田和夫、安田年博:
    • Organizer
      第34回日本法医学会中四国地方会
    • Related Report
      2017 Research-status Report
  • [Presentation] DNA polymerase β(POLB)遺伝子多型の世界分布2017

    • Author(s)
      木村かおり、藤原純子、山田和夫、安田年博、竹下治男
    • Organizer
      日本DNA多型学会第26回学術集会
    • Related Report
      2017 Research-status Report
  • [Presentation] Endoplasmic reticulum aminopeptidase 1 (ERAP1)遺伝子におけるSNP(rs27434)と臓器重量等との関連2017

    • Author(s)
      山田和夫、藤原純子、竹下治男、木村かおり、安田年博
    • Organizer
      日本DNA多型学会第26回学術集会
    • Related Report
      2017 Research-status Report
  • [Presentation] Defect and impurity analysis in GaSb thin films on Ge(100) substrates through rapid thermal annealing.2017

    • Author(s)
      Nishimoto N, Fujihara J, Yoshino K
    • Organizer
      29th International Conference on Defects in Semiconductors.
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research

URL: 

Published: 2017-07-21   Modified: 2022-01-27  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi