Development of a novel therapy method for the treatment of influenza using liposomes expressing sialic acids and alpha-galactose epitopes

• Project/Area Number: 17K19878
• Research Category: Grant-in-Aid for Challenging Research (Exploratory)
• Allocation Type: Multi-year Fund
• Research Field: Health science and related fields
• Research Institution: Obihiro University of Agriculture and Veterinary Medicine
• Principal Investigator: OGAWA HARUKO 帯広畜産大学, 畜産学部, 教授 (10400079)
• Research Collaborator: TAKEDA Yohei ; GALILI Uri
• Project Period (FY): 2017-06-30 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000); Fiscal Year 2018: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2017: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: インフルエンザウイルス / リポソーム / シアル酸 / α-ガラクトース / 自然抗体 / インフルエンザ

Outline of Final Research Achievements

This study aims to develop a novel inhalation therapy for influenza by using liposomes that are small particles composed mainly of lipids. The liposomes used for the therapy express two types of carbohydrate antigens so that immune response against influenza virus can be initiated quickly in humans after the influenza virus infection. Using the mice as the model animals, effectiveness of the liposome inhalation treatment to influenza virus infection was confirmed. Further study is ongoing to increase the efficacy of therapy.

Academic Significance and Societal Importance of the Research Achievements

季節性インフルエンザは、健常人で重篤となることは稀だが、免疫機能が低い幼児や高齢者では重症化しやすいハイリスク群である。本研究は、インフルエンザに対して自分で実施可能な新たな治療法の開発を目指しており、ハイリスク群の重症化防止に貢献できる可能性がある。また、急速に進む少子高齢化社会において、医療を必要とする重症患者の数を減らすことにつながり、医療費高騰による国の財政圧迫を軽減できる可能性がある。

Research Products

• [Journal Article] First detection of influenza A virus genes from wild raccoons in Japan (2018)
  • Author(s): Yamaguchi Emi、Fujii Kei、Ogawa Haruko、Imai Kunitoshi
  • Journal Title: Virus Genes Volume: 未定 Issue: 4 Pages: 591-595
  • DOI: 10.1007/s11262-018-1566-z
  • Peer Reviewed / Open Access
• [Journal Article] Characterization of cross-clade monoclonal antibodies against H5N1 highly pathogenic avian influenza virus and their application to the antigenic analysis of diverse H5 subtype viruses (2017)
  • Author(s): Gronsang Dulyatad、Bui Anh N.、Trinh Dai Q.、Bui Vuong N.、Nguyen Khong V.、Can Minh X.、Omatsu Tsutomu、Mizutani Tetsuya、Nagai Makoto、Katayama Yukie、Thampaisarn Rapeewan、Ogawa Haruko、Imai Kunitoshi
  • Journal Title: Archives of Virology Volume: 162 Issue: 8 Pages: 2257-2269
  • DOI: 10.1007/s00705-017-3350-0
  • NAID: 120006407582
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Evaluation of the replication and pathogenicity of a variant avian paramyxovirus serotype 6 in mice (2017)
  • Author(s): Bui Vuong Nghia、Trinh Dai Quang、Abao Lary N. B.、Ozeki Yuki、Runstadler Jonathan、Nakamura Kikuyasu、Ogawa Haruko、Imai Kunitoshi
  • Journal Title: Archives of Virology Volume: 162 Issue: 10 Pages: 3035-3043
  • DOI: 10.1007/s00705-017-3459-1
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Potential of electrolyzed water for disinfection of foot-and-mouth disease virus. (2017)
  • Author(s): Bui VN, Nguyen KV, Pham NT, Bui AN, Dao TD, Nguyen TT, Nguyen HT, Trinh DQ, Inui K, Uchiumi H, Ogawa H, Imai K.
  • Journal Title: J. Vet. Med. Sci. Volume: 79 Pages: 726-729
  • NAID: 120006391012
  • Peer Reviewed / Open Access / Int'l Joint Research