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Evaluation of skeletal muscle fiber type using photothermal microscopy

Research Project

Project/Area Number 17K19897
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Research Field Health science and related fields
Research InstitutionThe University of Electro-Communications

Principal Investigator

Kano Yutaka  電気通信大学, 大学院情報理工学研究科, 教授 (90293133)

Research Collaborator KOBAYASHI Takayoshi  
NAKATA Kazuaki  
TANAKA Hiroki  
Project Period (FY) 2017-06-30 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2018: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2017: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Keywords骨格筋 / 筋線維タイプ / ミトコンドリア / フォトサーマル顕微法 / 筋線維組成 / バイオイメージング
Outline of Final Research Achievements

Different fiber types of skeletal muscle (fast-twitch/slow-twitch) have different characteristics of mitochondrial distribution and its shape. Photothermal (PT) microscopes are imaged utilizing light absorbing molecules contained in tissue. In this study, we attempted to evaluate muscle fiber type from morphological characteristics of mitochondria using PT microscope. The PT imaging luminance values of SDH stained and unstained in slow muscle fibers were higher than those of fast muscle fibers. Slow muscle fibers showed many elongated mitochondrial shapes. On the other hand, the dotted mitochondrial shapes were seen in fast muscle fibers. These features were also confirmed in untreated muscle samples without muscle tissue fixation. Our original PT microscopic observation was shown to allow mitochondrial imaging in untreated muscle tissue samples.

Academic Significance and Societal Importance of the Research Achievements

骨格筋線維(筋細胞)は大きく速筋と遅筋に分類され,神経系や代謝的な特長が決定される.骨格筋研究において,筋線維タイプを分類することは基礎的な生体情報として重要である.これまで,筋線維タイプを生きたまま生体内で分類する方法は開発されていなかった.本研究はフォトサーマル(光熱変換)顕微法によって,線維タイプ別にミトコンドリア含有量や形態特性の違いを評価することに成功し,速筋と遅筋線維を生体内で同定することが可能であることを見いだした.

Report

(3 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • Research Products

    (6 results)

All 2018 2017 Other

All Int'l Joint Research (1 results) Journal Article (3 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 3 results,  Open Access: 2 results) Presentation (1 results) Remarks (1 results)

  • [Int'l Joint Research] カンザス州立大学(米国)

    • Related Report
      2018 Annual Research Report
  • [Journal Article] Muscle hypertrophy following blood flow-restricted low force isometric electrical stimulation in rat tibialis anterior: Role for muscle hypoxia.2018

    • Author(s)
      Nakajima T, Koide S, Yasuda T, Hasegawa T, Yamasoba T, Obi S, Toyoda S, Nakamura F, Inoue T, Poole DC, Kano Y
    • Journal Title

      J Appl Physiol

      Volume: 印刷中 Issue: 1 Pages: 134-145

    • DOI

      10.1152/japplphysiol.00972.2017

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Microvascular Permeability of Skeletal Muscle After Eccentric Contraction-Induced Muscle Injury: In Vivo Imaging Using Two-Photon Laser Scanning Microscopy2018

    • Author(s)
      Kazuki Hotta, Bradley Behnke, Kazuto Masamoto, Rie Shimotsu, Naoya Onodera, Akihiko Yamaguchi, David Poole, Yutaka Kano
    • Journal Title

      Journal of Applied Physiology

      Volume: 125 Issue: 2 Pages: 369-380

    • DOI

      10.1152/japplphysiol.00046.2018

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Photothermal imaging of skeletal muscle mitochondria2017

    • Author(s)
      Toru Tomimatsu, Jun Miyazaki, Yutaka Kano, and Takayoshi Kobayashi
    • Journal Title

      Biomedical Optics Express

      Volume: 8 Issue: 6 Pages: 2965-2975

    • DOI

      10.1364/boe.8.002965

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] フォトサーマル顕微鏡を用いた骨格筋細胞内のミトコンドリア形態観察2018

    • Author(s)
      田中宏樹,堀川大空,中田和明,小林孝嘉,狩野豊
    • Organizer
      2018日本体力医学会 学会大会
    • Related Report
      2018 Annual Research Report
  • [Remarks] 電気通信大学 基盤理工学専攻 狩野研究室

    • URL

      http://www.ecc.es.uec.ac.jp/index.html

    • Related Report
      2017 Research-status Report

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Published: 2017-07-21   Modified: 2020-03-30  

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