| Budget Amount *help |
¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2019: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2017: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
|
|---|
| Outline of Final Research Achievements |
When we develop a primary hepatocyte in low oxygen condition from the beginning, the cellular motility of final hepatocyte is lower than when we conduct those strategy in normoxic condition. This method provided us to higher sensitivity for exogenous stimulus including oxidative stress and ER stress. In addition, the hepatocyte that was prepared in normoxic condition has lower levels of stress transcriptional factor, Nrf1 and Nrf2. This condition leads the hepatocyte to lower response fate for exogenous stimulus and lower detoxification activity. We have found that the glycomodifier enzyme, PNGase was contributed to controlling Nrf1 protein level during primary hepatocyte preparation in lower oxygen condition.
|
