Functional analysis of novel myokine
| Project/Area Number |
17K19920
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| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Health science and related fields
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| Research Institution | Tokyo Metropolitan University |
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Principal Investigator |
Manabe Yasuko 首都大学東京, 人間健康科学研究科, 准教授 (60467412)
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| Project Period (FY) |
2017-06-30 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2019: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 骨格筋 / マイオカイン |
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| Outline of Final Research Achievements |
This study was investigated whether a new myokine candidate #30020 is actually synthesized in the skeletal muscle, secreted and played physiological roles. #30020 gene was expressed in human skeletal muscle cells, but not mouse skeletal muscle cells. When HA-tagged #30020 gene was overexpressed in mouse skeletal muscle by in vivo electroporation, #30020 protein expression was detected by an HA-antibody. To detect endogenous #30020, a direct antibody against #30020 was produced. Although overexpressed #30020 was detected by the HA-antibody, endogenous #30020 could not be detected by the direct antibody. We also tried to detect secretion of #30020 in the supernatant of human skeletal muscle cells, but could not detect any signal of #30020. These results suggest that #30020 is unlikely to be a new myokine.
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| Academic Significance and Societal Importance of the Research Achievements |
ヒト骨格筋特異的に発現し、分泌構造を有するタンパク質＃30020が新規のマイオカイン候補として挙がっていたため、複数の方法を用いてその可能性を検証した。しかし、期待されたようなタンパク質ではないことが明らかとなった。一方、本候補分子の検出や機能の解明のために用いた実験手法は、現在別のマイオカインの検証に役立っている。現在行っているマイオカイン候補は、大変興味深い結果を得ているため、今回の研究を進めるにあたって使用した手法が他のマイオカインの研究に役立っているという点で、大きな学術的意義があったと考える。
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Report
(4 results)
Research Products
(40 results)
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[Journal Article] R3hdml regulates satellite cell proliferation and differentiation2019
Author(s)
Sakamoto Kenichi、Furuichi Yasuro、Yamamoto Masashi、Takahashi Megumi、Akimoto Yoshihiro、Ishikawa Takahiro、Shimizu Takahiko、Fujimoto Masanori、Takada‐Watanabe Aki、Hayashi Aiko、Mita Yoshitaka、Manabe Yasuko、Fujii Nobuharu L、Ishibashi Ryoichi、Maezawa Yoshiro、Betsholtz Christer、Yokote Koutaro、Takemoto Minoru
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Journal Title
EMBO reports
Volume: 20
Issue: 11
Pages: 1-12
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] 新規遺伝子R3hdmlは筋衛星細胞の増殖能を制御し、骨格筋の分化再生を促進する2018
Author(s)
坂本 憲一, 竹本 稔, 古市 泰郎, 高橋 恵, 秋元 義弘, 山本 雅, 石川 崇広, 前澤 善朗, 清水 孝彦, 眞鍋 康子, 藤井 宣晴, 横手 幸太郎
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Journal Title
日本内分泌学会雑誌
Volume: 94
Pages: 292-292
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[Presentation] Single-cell information analysis reveals small intracellular and large intercellular variations increase cellular information capacity2019
Author(s)
Takumi Wada, Mitsutaka Wataya, Masashi Fujii, Ken-ichi Hironaka, Miki Eto, Shinsuke Uda, Daisuke Hoshino, Katsuyuki Kunida, Haruki Inoue, Hiroyuki Kubota Hiroki, Hamaguchi, Yasuro Furuichi , Yasuko Manabe, Nobuharu L. Fujii, Shinya Kuroda
Organizer
The 20th International Conference on System Biology (ICSB2019I)
Related Report
Int'l Joint Research
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[Presentation] 新規筋衛星細胞発現遺伝子R3hdmlは筋衛星細胞の増殖能を制御し、骨格筋の分化再生を促進する2018
Author(s)
坂本憲一, 竹本稔, 古市泰郎, 高橋恵, 秋元義弘, 山本雅, 石川崇広, 前澤善朗, 清水 孝彦, 眞鍋 康子, 藤井 宣晴, 横手 幸太郎
Organizer
第61回日本糖尿病学会
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