| Budget Amount *help |
¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2019: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2018: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2017: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Outline of Final Research Achievements |
We examined the utilization of dissolved oxygen by Rhodococcus bacteria in the alkane phase of two-phase cultures in order to clarify bacterial growth mechanisms in the presence of alkanes. The results showed that the dissolved oxygen in the alkane phase remarkably decreased when viable cells were present in the alkane phase, irrespective of the kind of alkane or Rhodococcus strain tested. Thus, our findings indicate that Rhodococcus strains can grow in the alkane phase by utilizing the dissolved oxygen. To the best of our knowledge, this is the first study to experimentally demonstrate that translocated cells in organic solvents utilize the dissolved oxygen in the inorganic solvent for growth.
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