EFFECTS OF MECHANICAL STIMULUS ON BEAT SEQUENCE OF CARDIOMYOCYTES WITH FEEDBACK CONTROL
| Project/Area Number |
17K20113
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| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biomedical engineering and related fields
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| Research Institution | Aoyama Gakuin University |
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Principal Investigator |
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| Project Period (FY) |
2017-06-30 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
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| Keywords | 心臓 / リズム / 不整脈 / 同期化 / リエントラント / 心筋細胞 / 自律拍動 / メカノバイオロジー / 同期現象 / リアルタイムプロセス / 同期 / 発達 / 刺激 / 生理学 / マイクロ・ナノデバイス / 生物物理 |
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| Outline of Final Research Achievements |
We presented the beat dynamics in sub-mm tissue fragments from atria and ventricles of hearts from chick embryos. The inter-beat intervals and the response to mechanical stimulus are different by the regions. The major finding of this study is synchronization of these fragment pairs physically attached to each other. The probability of achieving this and the time required differ for regional pairs: atrium-atrium, ventricle-ventricle, or atrium-ventricle. Our observation indicates that mechanical stimulus at fragment boundaries induces synchronization. A reentrant-like beat sequence, applicable to testing drugs, is frequently observed during synchronization. To investigate the effects by mechanical stimulus further, we built an instrument applying probe stimuli to cardiac cells. In addition, we successfully added a feedback control to adjust the phase between stimulus and intrinsic beating by fast image processing. This novel instrument shows the phase differences of the responses.
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| Academic Significance and Societal Importance of the Research Achievements |
胎児期における心臓のメカノセンシングは発生の形状変化において重要と示唆されてきた。我々は組織片ペアの同期化の実験結果において、ペアの接触面における動きが同期化に重要であることを発見した。これは直接的に動きがリズムの安定化に寄与することを示す。また、心房―心室のリエントラント的振る舞いはin vitroの系としてドラッグテスト等への応用が期待できる。次に培養環境下の長期的観測と高速画像処理により構築したフィードバック機構付きの外部刺激装置の完成は筆者の知る限り世界初である。この装置による自律拍動と常時同調した刺激により、発生に近い集団的心筋細胞の形状変化や線維芽細胞の異方的増殖を期待する。
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Report
(5 results)
Research Products
(26 results)
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[Journal Article] Biochemical study of type I collagen purified from skin of warm sea teleost Mahi mahi (Coryphaena hippurus), with a focus on thermal and physical stability.2019
Author(s)
Akita, M, Kono, T, Lloyd, K, Mitsui, T, Morioka, K, Adachi, K
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Journal Title
J Food Biochem
Volume: 43
Issue: 11
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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