Development and scientific creation of structured gene carriers based on controlled higher-order structures of pDNA

• Project/Area Number: 17KK0102
• Research Category: Fund for the Promotion of Joint International Research (Fostering Joint International Research)
• Allocation Type: Multi-year Fund
• Research Field: Biomedical engineering/Biomaterial science and engineering
• Research Institution: National Institutes for Quantum Science and Technology (2018-2023); The University of Tokyo (2017)
• Principal Investigator: 長田 健介 国立研究開発法人量子科学技術研究開発機構, 量子医科学研究所 分子イメージング診断治療研究部, グループリーダー (10396947)
• Project Period (FY): 2018 – 2023
• Project Status: Completed (Fiscal Year 2023)
• Budget Amount: ¥14,950,000 (Direct Cost: ¥11,500,000、Indirect Cost: ¥3,450,000)
• Keywords: 薬物送達システム / 非ウイルス性遺伝子デリバリーシステム / 高分子ミセル / ポリイオンコンプレックス / DNA凝縮 / 核酸治療 / 非ウイルス性遺伝子ベクター / 高分子集合体

Outline of Annual Research Achievements

本課題では、DNAと正電荷性高分子からなる複合体の高次構造制御とそれを遺伝子ベクターとして医療応用しようとする基研究を、ナノメディシンの臨床応用研究を進めるグループとの協議を通じてステップアップを図る（課題(1)）とともに、生物物理学のグループと共同することで高次構造形成の基礎学術を深化させること（課題(2)）、さらに新たな核酸治療システムを構築すること（課題(3)）を目的としている。
課題(1)では、高分子の設計、合成からナノメディシンの作製、動物評価、企業への受け渡しまで行っているドイツ・マインツ大学のRudorf Zentel教授・Matthias Barz教授のもとで、開発現場から企業への橋渡しまでのプロセスを共有した。感染症蔓延のため渡航が途絶えていたが、23年度はオランダ・ライデン大学に移動したMatthias Barz教授の研究室に滞在し、ヨーロッパの最先端事情と今後の課題を議論した。課題(2)は、本国際共同研究の交流により知り合ったインド国立計算科学研究所のグループと核酸－ペプチド複合体の形成プロセスのシミュレーションを進め、DNA/正電荷性高分子複合体をより安定化する高分子に関して、原子レベルにおける会合挙動から示唆を得た。これを元に、核酸を全身投与でがん細胞に送達し、制がん剤の薬効を飛躍的に高める新たな核酸治療の開発に展開している（課題(3)）。また、基課題を発展させた系として、がん免疫と協働する新たな遺伝子免疫治療戦略の開発に着手し、強力な制がん効果が得られることを確認した。感染症の影響により渡航を延期せざるを得ず、当初予定した渡航期間に満たなかったが、メールやオンラインでのディスカッションを取り入れながら研究を進めることで本研究課題を完了させることができた。

Research Products

• [Int'l Joint Research] ライデン大学(オランダ) (2024)
  • Year and Date: 2024-02-19
• [Int'l Joint Research] Dalian University of Technology(中国) (2019)
  • Year and Date: 2019-12-06
• [Int'l Joint Research] ミュンヘン大学(ドイツ) (2019)
  • Year and Date: 2019-08-23
• [Int'l Joint Research] Kent State University, Ohio(米国) (2019)
  • Year and Date: 2019-07-10
• [Int'l Joint Research] VISTECH(タイ) (2019)
  • Year and Date: 2019-04-03
• [Int'l Joint Research] マインツ大学(ドイツ) (2019)
• [Int'l Joint Research] ドレスデン工科大学(ドイツ) (2018)
• [Journal Article] Block copolymer-stabilized MOF hybrids loading Pd nanoparticles enable tumor remission through near-infrared photothermal therapy (2024)
  • Author(s): Shang-Wei Li, Ming-Feng Hsieh, Taehun Hong, Pengwen Chen, Kensuke Osada, Xueying Liu, Ichio Aoki, Jiashing Yu, Kevin C.-W. Wu, Horacio Cabral
  • Journal Title: Advanced NanoBiomed Research Volume: - Issue: 1
  • DOI: 10.1002/anbr.202470011
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Quantification of poly(ethylene glycol) crowding on nanodiamonds toward quantum biosensor for improved prevention effects on protein adsorption and lung accumulation (2024)
  • Author(s): K. Rikiyama, N. Maehara, H. Abe, Y. Nishimura, H. Yukawa, K. Kaminaga, R. Igarashi, K. Osada
  • Journal Title: Langmuir Volume: - Issue: 18 Pages: 9471-9480
  • DOI: 10.1021/acs.langmuir.3c03988
  • Peer Reviewed
• [Journal Article] Self-Folding Macromolecular Drug Carrier for Cancer Imaging and Therapy (2023)
  • Author(s): S. Gao, Y. Miura, A. Sumiyoshi, S. Ohno, K. Ogata, T. Nomoto, M. Matsui, Y. Honda, M. Suzuki, M. Iiyama, K. Osada, I. Aoki, N. Nishiyama
  • Journal Title: Advanced Science Volume: 11 Issue: 7 Pages: 2304171-2304171
  • DOI: 10.1002/advs.202304171
  • Peer Reviewed / Open Access
• [Journal Article] Size-tunable PEG-grafted copolymers as a polymeric nanoruler for passive targeting muscle tissues. (2022)
  • Author(s): M. Naito, Y. Watanuki, K. Toh, J. Yum, B. S. Kim, K. Taniwaki, S. Ogura, H. Ishida, M. Cho, H. Chaya, K. Miyajima, K. Osada, K. Minegishi, Y. Aoki, Y. Yamasaki, K. Miyata,
  • Journal Title: Journal of Controlled Release Volume: 347 Pages: 607-614
  • DOI: 10.1016/j.jconrel.2022.05.030
  • Peer Reviewed / Open Access
• [Journal Article] Delivery of aPD-L1 antibody to i.p. tumors via direct penetration by i.p. route: beyond EPR effect. (2022)
  • Author(s): M. Yamamoto, T. Kurino, R. Matsuda, H. S. Jones, Y. Nakamura, T. Kanamori, A. B. Tsuji, A. Sugyo, R. Tsuda, Y. Matsumoto, Y. Sakurai, H. Suzuki, M. Sano, K. Osada, T. Uehara, Y. Ishii, H. Akita, Y. Arano, A. Hisaka, H. Hatakeyama,
  • Journal Title: Journal of Controlled Release Volume: 352 Pages: 328-337
  • DOI: 10.1016/j.jconrel.2022.10.032
  • Peer Reviewed
• [Journal Article] Size-controlled bimodal in vivo nanoprobes as near-infrared phosphors and positive contrast agents for magnetic resonance imaging (2021)
  • Author(s): K. Okubo, R. Takeda, S. Murayama, M. Umezawa, M. Kamimura, K. Osada, I. Aoki, K. Soga,
  • Journal Title: Science and Technology of Advanced Materials Volume: 22 Issue: 6 Pages: 160-172
  • DOI: 10.3390/pharmaceutics13060800
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Bridging mRNA and polycation using RNA oligonucleotide derivatives improves the robustness of polyplex micelle for efficient mRNA delivery. (2021)
  • Author(s): N. Yoshinaga, S. Uchida, A. Dirisala, M. Naito, K. Koji, K. Osada, H. Cabral, K. Kataoka,
  • Journal Title: Advanced Healthcare Materials, Volume: - Issue: 9 Pages: 2102016-2102016
  • DOI: 10.1002/adhm.202102016
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] mRNA loading into ATP-responsive polyplex micelles with optimal density of phenylboronate ester crosslinking to balance robustness in the biological milieu and intracellular translational efficiency (2021)
  • Author(s): Yoshinaga Naoto、Uchida Satoshi、Dirisala Anjaneyulu、Naito Mitsuru、Osada Kensuke、Cabral Horacio、Kataoka Kazunori
  • Journal Title: Journal of Controlled Release Volume: 330 Pages: 317-328
  • DOI: 10.1016/j.jconrel.2020.12.033
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Transient stealth coating of liver sinusoidal wall by anchoring two-armed PEG for retargeting nanomedicines (2020)
  • Author(s): Dirisala Anjaneyulu、Uchida Satoshi、Toh Kazuko、Li Junjie、Osawa Shigehito、Tockary Theofilus A.、Liu Xueying、Abbasi Saed、Hayashi Kotaro、Mochida Yuki、Fukushima Shigeto、Kinoh Hiroaki、Osada Kensuke、Kataoka Kazunori
  • Journal Title: Science Advances Volume: 6 Issue: 26
  • DOI: 10.1126/sciadv.abb8133
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Bundling of mRNA strands inside polyion complexes improves mRNA delivery efficiency in vitro and in vivo (2020)
  • Author(s): Koji Kyoko、Yoshinaga Naoto、Mochida Yuki、Hong Taehun、Miyazaki Takuya、Kataoka Kazunori、Osada Kensuke、Cabral Horacio、Uchida Satoshi
  • Journal Title: Biomaterials Volume: 261 Pages: 120332-120332
  • DOI: 10.1016/j.biomaterials.2020.120332
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Structural Polymorphism of Single pDNA Condensates Elicited by Cationic Block Polyelectrolytes (2020)
  • Author(s): Osada Kensuke
  • Journal Title: Polymers Volume: 12 Issue: 7 Pages: 1603-1603
  • DOI: 10.3390/polym12071603
  • NAID: 120006882399
  • Peer Reviewed / Open Access
• [Journal Article] 膵臓がんの間質を越える一本鎖ＤＮＡ内包高分子ベクター (2020)
  • Author(s): 長田健介
  • Journal Title: バイオマテリアル Volume: 38 Pages: 196-197
  • Peer Reviewed
• [Journal Article] Single-stranded DNA-packaged polyplex micelle as adeno-associated-virus-inspired compact vector to systemically target stroma-rich pancreatic cancer. (2019)
  • Author(s): T. A. Tockary, W. Foo, A. Dirisala, Q. Chen, S. Uchida, S. Osawa, Y. Mochida, X. Liu, H. Kinoh, H. Cabral, K. Osada, K. Kataoka
  • Journal Title: ACS Nano Volume: 13 Issue: 11 Pages: 12732-12742
  • DOI: 10.1021/acsnano.9b04676
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] In vivo rendezvous of small nucleic acid drugs with charge-matched block catiomers to target cancers (2019)
  • Author(s): Watanabe Sumiyo、Hayashi Kotaro、Toh Kazuko、Kim Hyun Jin、Liu Xueying、Chaya Hiroyuki、Fukushima Shigeto、Katsushima Keisuke、Kondo Yutaka、Uchida Satoshi、Ogura Satomi、Nomoto Takahiro、et al.
  • Journal Title: Nature Communications Volume: 10 Issue: 1 Pages: 1894-1894
  • DOI: 10.1038/s41467-019-09856-w
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Bundling mRNA strands to prepare nano-assemblies with enhanced stability towards RNase for in vivo delivery. (2019)
  • Author(s): N. Yoshinaga, E. Cho, K. Koji, Y. Mochida, M. Naito, K. Osada, K. Kataoka, H. Cabral, S. Uchida
  • Journal Title: Angew. Chem. Int. Ed. Volume: 58 Issue: 33 Pages: 11360-11363
  • DOI: 10.1002/anie.201905203
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Precise tuning of disulfide crosslinking in mRNA polyplex micelles for optimizing extracellular and intracellular nuclease tolerability. (2019)
  • Author(s): A. Dirisala, S. Uchida, T. A. Tockary, N. Yoshinaga, J. Li, S. Osawa, L. Gorantla, S. Fukushima, K. Osada, K. Kataoka
  • Journal Title: J. Drug Target. Volume: 印刷中 Issue: 5-6 Pages: 670-680
  • DOI: 10.1080/1061186x.2018.1550646
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Induced packaging of mRNA into polyplex micelles by regulated hybridization with a small number of cholesteryl RNA oligonucleotides directed enhanced in vivo transfection. (2019)
  • Author(s): N. Yoshinaga, S. Uchida, M. Naito, K. Osada, H. Cabral, K. Kataoka
  • Journal Title: Biomaterials Volume: 197 Pages: 255-267
  • DOI: 10.1016/j.biomaterials.2019.01.023
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Versatile DNA folding structures organized by cationic block copolymers (2019)
  • Author(s): Osada Kensuke
  • Journal Title: Polymer Journal Volume: 51 Issue: 4 Pages: 381-387
  • DOI: 10.1038/s41428-018-0157-0
  • NAID: 40021935767
  • Peer Reviewed
• [Journal Article] Block Copolymer Micelles in Nanomedicine Applications (2018)
  • Author(s): Cabral Horacio、Miyata Kanjiro、Osada Kensuke、Kataoka Kazunori
  • Journal Title: Chemical Reviews Volume: 118 Issue: 14 Pages: 6844-6892
  • DOI: 10.1021/acs.chemrev.8b00199
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] Liver sinusoidal wall coating polymer to promote the efficacy of gene therapy drugs, The 40th International Conference of Photopolymer Science and Technology (2023)
  • Author(s): K. Osada
  • Organizer: The 40th International Conference of Photopolymer Science and Technology
  • Int'l Joint Research / Invited
• [Presentation] Polymeric Architectures to Perform Virus Functions and its Challenge to Pancreatic Cancer (2023)
  • Author(s): K. Osada
  • Organizer: International Conference on Precision Nanomedicine in Theranostics & The 2023 Annual Meeting of Taiwan Nanomedicine Society
  • Int'l Joint Research / Invited
• [Presentation] ナノダイヤモンドの定量的PEG化 (2023)
  • Author(s): 長田健介
  • Organizer: 量子生命科学先端フォーラム2023夏の研究会
• [Presentation] 生体量子センシングのためのナノダイヤモンドの定量的PEG化 (2023)
  • Author(s): 長田健介, 力山和晃
  • Organizer: 第72回高分子討論会
  • Invited
• [Presentation] 日本学術振興会特別研究員応募書類作成で気をつけたいこと (2023)
  • Author(s): 長田健介
  • Organizer: NITech PGCプログラム日本学術振興会特別研究員申請支援セミナー
  • Invited
• [Presentation] 高分子で挑む膵臓がんの遺伝子/核酸治療 (2023)
  • Author(s): 長田健介
  • Organizer: 増殖制御遺伝子TOB研究会
• [Presentation] Quantification of PEG crowding grafted onto nanodiamonds (2023)
  • Author(s): K. Osada
  • Organizer: The 11th Conference on Exploring Next-Generation Materials Science and Nanoscience (11thCENG-MSN) and Workshop on Soft and Nano Materials Orchestrated with Wisdom from Japan 2024 (SNOWJ2024)
  • Int'l Joint Research
• [Presentation] Nano-Ruler MRI contrast agent to detect inflammatory vasculature (2023)
  • Author(s): K. Osada, N. Maehara, A. Sumiyoshi, M. Naito, K. Miyata, I. Aoki,
  • Organizer: The 11th Takeda Science Foundation Symposium on Pharmasciences, Congres Convention Center
  • Int'l Joint Research
• [Presentation] Nano-Ruler MRI contrast agent to detect inflammatory vasculature (2023)
  • Author(s): K. Osada
  • Organizer: MRI Symposium Osaka
  • Int'l Joint Research
• [Presentation] Transient stealth coating of liver sinusoidal wall by PEG for retargeting nanomedicines (2023)
  • Author(s): K. Osada
  • Organizer: 1st Workshop on Soft and Nano Materials Orchestrated with Wisdom from Japan and Korea 2024
  • Int'l Joint Research
• [Presentation] 生体量子センシングのためのナノダイヤモンドの定量的PEG化 (2023)
  • Author(s): 長田健介
  • Organizer: 第13回ナノバイオ若手ネットワーキングシンポジウム
• [Presentation] Polymers for cancer therapy and diagnosis (2023)
  • Author(s): K. Osada
  • Organizer: Seminar in Division of BioTherapeutics, Leiden Univeristy
  • Int'l Joint Research / Invited
• [Presentation] Artificial viruses to target cancers by polymer nanoassembly technology (2023)
  • Author(s): 長田健介
  • Organizer: OIST Seminar
  • Int'l Joint Research / Invited
• [Presentation] 化学で生命に迫る ～高分子化学でがんに挑む (2022)
  • Author(s): 長田健介
  • Organizer: 東進ハイスクール未来発見講座
  • Invited
• [Presentation] ナノダイヤモンドの定量的PEG化の試み (2022)
  • Author(s): 長田健介
  • Organizer: 量子計測技術による細胞生物物理学の進展と生命のパラメトリク生理機能の解明への展開
  • Invited
• [Presentation] がん微小環境を診る高分子造影剤の開発とPDXモデルへの適用 (2022)
  • Author(s): 長田健介, 力山和晃, 綿貫裕介, 内藤瑞, 宮田完二郎, 住吉晃, 青木伊知男
  • Organizer: 第38回日本DDS学会
• [Presentation] がん微小環境を診る高分子造影剤の開発とPDXモデルへの適用 (2022)
  • Author(s): 長田健介
  • Organizer: 第71回高分子討論会
  • Invited
• [Presentation] 高分子化学を道具にがんに挑戦する試みとナノダイヤモンド量子計測への展開 (2022)
  • Author(s): 長田健介
  • Organizer: 大阪大学蛋白研セミナー
  • Invited
• [Presentation] 高分子化学を道具にがんに挑戦する研究 (2022)
  • Author(s): 長田健介
  • Organizer: 茨城県立水戸第一高等学校大学模擬講義
  • Invited
• [Presentation] がん微小環境を診る高分子造影剤の開発とPDXモデルへの適用 (2022)
  • Author(s): 長田健介, 力山和晃, 綿貫裕介, 内藤瑞, 宮田完二郎, 住吉晃, 青木伊知男
  • Organizer: 第44回バイオマテリアル学会大会
• [Presentation] A safe liver sinusoidal wall coating agent to promote the efficacy of gene therapy drugs (2022)
  • Author(s): 長田健介
  • Organizer: International Symposium on Chemistry 2022 and the 2nd Kyutech-KKU International Symposium
  • Int'l Joint Research / Invited
• [Presentation] A safe liver sinusoidal wall coating agent to promote the efficacy of gene therapy drugs (2022)
  • Author(s): 長田健介
  • Organizer: The 5th International Conference on Nanospace Materials 2022
  • Int'l Joint Research / Invited
• [Presentation] Through the stromal barrier to pancreatic cancer cell nests: Single-stranded DNA-packaged polyplex micelle as AAV-inspired compact gene vector (2022)
  • Author(s): 長田健介
  • Organizer: The 5th International Workshop by the 174th Committee on Coexistence of Biology and Nanodevices
  • Int'l Joint Research / Invited
• [Presentation] A safe liver sinusoidal wall coating agent to promote the efficacy of gene therapy drugs (2022)
  • Author(s): 長田健介
  • Organizer: The 4th International Conference on Materials Research and Innovation
  • Int'l Joint Research / Invited
• [Presentation] 化学で生命を作る挑戦 (2022)
  • Author(s): 長田健介
  • Organizer: MRI Collaborator’s Workshop 2022
  • Invited
• [Presentation] ナノメディシンの送達効率を高める肝類洞内皮壁高分子コーティング剤 (2021)
  • Author(s): 長田健介, Anjaneyulu Dirisala, 内田智士, 片岡一則
  • Organizer: 第15回日本分子イメージング学会
• [Presentation] 膵臓がんの間質を超える一本鎖DNA内包ポリプレックスミセル (2021)
  • Author(s): 長田健介, Theofilus A. Tockary, 片岡一則
  • Organizer: 第37回日本DDS学会学術集会
• [Presentation] 高分子で作る人工ウイルスと遺伝子治療への展開 (2021)
  • Author(s): 長田健介
  • Organizer: CSJ化学フェスタ
  • Invited
• [Presentation] DNAをたたむ、遺伝子を発現させる、病気を治す (2021)
  • Author(s): 長田健介
  • Organizer: 茨城県立水戸第一高等学校大学模擬講義
  • Invited
• [Presentation] 非ウイルス性遺伝子治療 ～新しい遺伝子ベクターの設計と医療への応用～ (2021)
  • Author(s): 長田健介
  • Organizer: BioJapan
• [Presentation] 核酸デリバリーシステムにおける核酸とポリアミノ酸との相互作用解析 (2021)
  • Author(s): 長田健介
  • Organizer: BINDS NMR報告会
• [Presentation] 生体計測を目指したナノダイヤモンド量子センサーの開発 (2021)
  • Author(s): 長田健介
  • Organizer: スイゼンジノリ・サクラン研究会
  • Invited
• [Presentation] ナノダイヤモンドを用いた生体ナノ量子センサーの開発 (2021)
  • Author(s): 長田健介
  • Organizer: 福岡工業大学総合研究機構エレクトロニクス研究所講演会
  • Invited
• [Presentation] Single-stranded DNA-packaged Polyplex Micelle as AAV-inspired Compact Gene Vector to Systemically Target Stroma-rich Pancreatic Cancer (2021)
  • Author(s): 長田健介
  • Organizer: ICMARI2021
  • Int'l Joint Research / Invited
• [Presentation] DDSの基礎と応用 (2020)
  • Author(s): 長田健介
  • Organizer: 量子生命科学先端フォーラム2020夏の勉強会
  • Invited
• [Presentation] Strategic design of polyplex micelles toward systemic gene delivery (2020)
  • Author(s): Kensuke Osada
  • Organizer: Seminar, University of Michigan
  • Int'l Joint Research / Invited
• [Presentation] DNAをたたむ、遺伝子を発現させる、病気を治す (2020)
  • Author(s): 長田健介
  • Organizer: 茨城県立水戸第一高等学校大学模擬講義
  • Invited
• [Presentation] 一本鎖DNA内包ポリプレックスミセルによる膵臓がんの間質通過戦略 (2020)
  • Author(s): 長田健介, Theofilus A. Tockary, 片岡一則
  • Organizer: 第69回高分子学会年次大会
• [Presentation] Control of versatile higher-ordered structures of pDNA-block copolymer complexes and their application as gene delivery system (2019)
  • Author(s): Kensuke Osada
  • Organizer: Seminar at VISTEC
  • Int'l Joint Research / Invited
• [Presentation] Compartmentalization of single-stranded DNA from pDNA by polyplex micelles (2019)
  • Author(s): Kensuke Osada, Theofilus A. Tockary, Kazunori Kataoka
  • Organizer: 第67回高分子学会年次大会
• [Presentation] 高分子ミセルによる一本鎖DNAの隔離化 (2019)
  • Author(s): 長田健介
  • Organizer: NanoBio第12回若手ネットワーキングシンポジウム
• [Presentation] Control of versatile higher-ordered structures of pDNA by block copolymers and their application as gene delivery system (2019)
  • Author(s): Kensuke Osada
  • Organizer: Seminar at Institute of Polymers, Bulgaria Academy of Science, Sofia, Bulgaria,
  • Int'l Joint Research / Invited
• [Presentation] Control of versatile higher-ordered structures of pDNA by block copolymers and their application as gene delivery system (2019)
  • Author(s): Kensuke Osada
  • Organizer: Seminar at Institute of Macromolecular Chemistry, Freiburg University, Germany
  • Int'l Joint Research / Invited
• [Presentation] Control of versatile higher-ordered structures of pDNA by block copolymers and their application as gene delivery system (2019)
  • Author(s): Kensuke Osada
  • Organizer: Workshop on Nano-Bio interfaces
  • Int'l Joint Research / Invited
• [Presentation] 一本鎖DNA内包ポリプレックスミセルの遺伝子発現活性 (2019)
  • Author(s): 長田健介, Theofilus Tockary, 片岡一則
  • Organizer: 第68回高分子学会討論会
• [Presentation] DNA一本鎖は遺伝子発現するか (2019)
  • Author(s): 長田健介
  • Organizer: 第一回量子生命科学会若手の会
  • Invited
• [Presentation] 膵臓がんの間質を超える一本鎖DNA内包ポリプレックスミセル (2019)
  • Author(s): 長田健介, Theofilus A. Tockary, 片岡一則
  • Organizer: 第41回バイオマテリアル学会
  • Invited
• [Presentation] Compartmentalization of single-stranded DNA from pDNA by polyplex micelles (2019)
  • Author(s): Kensuke Osada, Theofilus A. Tockary, Kazunori Kataoka
  • Organizer: 2nd GLowing Polymer Symposium in KANTO (GPS-K 2019)
  • Int'l Joint Research / Invited
• [Presentation] Control of versatile higher-ordered structures of pDNA by block copolymers and their application as gene delivery system (2019)
  • Author(s): Kensuke Osada
  • Organizer: Seminar at Ningbo Institute of Material Technology and Engineering, Chinese Academy of Sciences
  • Int'l Joint Research / Invited
• [Presentation] Control of versatile higher-ordered structures of pDNA by block copolymers and their application as gene delivery system (2019)
  • Author(s): Kensuke Osada
  • Organizer: ICMARI 2019
  • Int'l Joint Research / Invited
• [Presentation] Control of higher ordered structures of pDNA-block copolymer complexes and their application for systemic gene therapy (2019)
  • Author(s): Kensuke Osada
  • Organizer: VISTEC special lectures 2019
  • Invited
• [Presentation] Control of versatile higher-ordered structures of pDNA-block copolymer complexes and their application as gene delivery system (2019)
  • Author(s): Kensuke Osada
  • Organizer: Lecture series by Collaborative Research Center 1066
  • Invited
• [Presentation] Control of versatile higher-ordered structures of pDNA-block copolymer complexes and their application as gene delivery system (2019)
  • Author(s): Kensuke Osada
  • Organizer: Seminar at Department of Chemistry, University of Strasbourg
  • Invited
• [Presentation] DNA-高分子複合体が創る多様な高次構造と医療への展開 (2018)
  • Author(s): 長田健介
  • Organizer: QST未来ラボセミナー
  • Invited
• [Presentation] DNA-高分子複合体が創る多様な高次構造と医療への展開 (2018)
  • Author(s): 長田健介
  • Organizer: 量子生命科学研究会第二回学術集会
• [Presentation] せん断応力に対するポリプレックスミセルの構造安定性 (2018)
  • Author(s): 長田健介、武田香織、山崎裕一、片岡一則
  • Organizer: 67回高分子学会年次大会
• [Presentation] ポリプレックスミセル対する血流せん断応力の影響 (2018)
  • Author(s): 長田健介、武田香織、山崎裕一、片岡一則
  • Organizer: 第 34回 DDS学会
• [Presentation] 高分子ミセルによる一本鎖DNAの隔離化 (2018)
  • Author(s): 長田健介, Theofilus Tockary, 片岡一則
  • Organizer: 第67回高分子学会討論会
• [Presentation] DNAをたたむ、遺伝子を発現させる (2018)
  • Author(s): 長田健介
  • Organizer: 茨城県立水戸第一高等学校大学模擬講義
  • Invited
• [Presentation] ウイルスの構造・機能に学ぶ高分子ミセル型遺伝子キャリアの開発 (2018)
  • Author(s): 長田健介
  • Organizer: 積水化学フォーラム
• [Presentation] DNAの折りたたみ制御と膵臓がんの遺伝子治療への展開 (2018)
  • Author(s): 長田健介
  • Organizer: 第35回医用高分子研究会講座
  • Invited
• [Presentation] Versatile higher ordered structures of pDNA-block copolymer complexes and their application as gene delivery system (2018)
  • Author(s): Kensuke Osada
  • Organizer: DNA-Mitteldeutschland Winter Meeting 2018
  • Int'l Joint Research / Invited
• [Presentation] Versatile higher ordered structures of pDNA-block copolymer complexes and their application as gene delivery system (2018)
  • Author(s): Kensuke Osada
  • Organizer: 1st GLowing Polymer Symposium in KANTO
  • Int'l Joint Research / Invited
• [Book] Drug Delivery System (2022)
  • Author(s): 長田健介
  • Total Pages: 10
  • Publisher: 日本DDS学会
• [Book] 高分子 (2021)
  • Author(s): 長田健介
  • Total Pages: 2
  • Publisher: 高分子学会
• [Book] Drug Delivery System (2021)
  • Author(s): 青木伊知男, 長田健介, 住吉晃, Bakalova Rumiana
  • Total Pages: 17
  • Publisher: 日本DDS学会
• [Book] 核酸科学ハンドブック (2020)
  • Author(s): 長田健介
  • Total Pages: 576
  • Publisher: 講談社
  • ISBN: 9784065207864
• [Book] ドラッグキャリア設計入門 (2019)
  • Author(s): 片岡 一則、原島 秀吉
  • Total Pages: 240
  • Publisher: 丸善出版
  • ISBN: 9784621303658
• [Book] Molecular Technology Vol. 2 Life Innovation (2018)
  • Author(s): Molecular technology for control of DNA folding within polyplex micelles by block catiomers toward systemic gene therapy
  • Total Pages: 400
  • Publisher: Wiley-VCH
• [Remarks] 遺伝子治療の効率を安全かつ大幅に高める 肝内毛細血管コーティング剤の開発
  • URL: https://www.qst.go.jp/site/press/41886.html
• [Patent(Industrial Property Rights)] 単一高分子からなる自己凝集型ミセル (2023)
  • Inventor(s): 三浦裕,西山伸宏,ガ オシャン,青木伊知 男,長田健介,住吉晃,
  • Industrial Property Rights Holder: 東京工業大学、量子科学技術研究開発機構
  • Industrial Property Rights Type: 特許
  • Filing Date: 2023
• [Patent(Industrial Property Rights)] 修飾済基材，修飾済基材の製造方法，及び，修飾済基材における重なり合い密度の評価方法 (2023)
  • Inventor(s): 長田健介, 力山和晃
  • Industrial Property Rights Holder: 量子科学技術研究開発機構
  • Industrial Property Rights Type: 特許
  • Filing Date: 2023
• [Patent(Industrial Property Rights)] 単一高分子粒子、活性分子複合体、単一高分子粒子の製造方法、腫瘍サイズの計測方法、腫瘍内の微細構造の計測方法、生体組織のイメージング方法、ドラッグデリバリーシステム、造影剤キット (2023)
  • Inventor(s): 長田健介, 力山和晃, 住吉晃, 青木伊知男, 内藤瑞, 等
  • Industrial Property Rights Holder: 長田健介, 力山和晃, 住吉晃, 青木伊知男, 内藤瑞, 等
  • Industrial Property Rights Type: 特許
  • Filing Date: 2023
  • Overseas
• [Patent(Industrial Property Rights)] 単一高分子粒子、活性分子複合体、単一高分子粒子の製造方法、腫瘍サイズの計測方法、腫瘍内の微細構造の計測方法、生体組織のイメージング方法、ドラッグデリバリーシステム、造影剤キット (2022)
  • Inventor(s): 長田健介, 力山和晃, 住吉晃, 青木伊知男, 内藤瑞, 等
  • Industrial Property Rights Holder: 長田健介, 力山和晃, 住吉晃, 青木伊知男, 内藤瑞, 等
  • Industrial Property Rights Type: 特許
  • Filing Date: 2022