| Outline of Final Research Achievements |
PEGs have been known as a bio-inert polymer, and conjugation of PEG on proteins, which is called PEGylation, is the most frequently used method for protein modification. Nowadays, PEG has been used for coverages of lipid nanoparticles for mRNA delivery. PEG reduces proteins’ immunogenicity, however, PEG-specific antibodies have been observed in patients, who received repeated injection of PEGylated therapeutics. Therefore, there is the issue that PEG-specific antibodies have been induced after injections of PEGylated therapeutics to be overcome. We have previously suggested a new PEGylation method to reduce PEG-related immunogenicity, and the current study examined the new PEGylation onto proteins. Our suggested PEGylation reduced PEG-related antibody responses, whereas a classic PEGylation exhibited PEG-related antibody responses. Our PEGylation method will be a promising new method for protein modification.
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