| Outline of Final Research Achievements |
Some secondary metabolites derived from Streptomyces show a wide variety of the biological activity, such as antiparasitic and antitumor. During a previous project, we got novel compounds produced by artificial enzymes based on indole prenyltransferase. In the other project, we determined the complex crystal structure of FtsZ, which is a critical role for bacterial cell division, with compound and revealed its inhibitory mechanism as well. Therefore, in this study, to evaluate the anti-FtsZ activity of the novel compounds, we used electron microscopy and LC-MS/MS, resulting that we developed a novel measurement procedure for identification of protein state using LC-MS/MS.
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