Chromoatin organization and transcription regulation during zebrafish zygotic genome activation

• Project/Area Number: 17KK0143
• Research Category: Fund for the Promotion of Joint International Research (Fostering Joint International Research)
• Allocation Type: Multi-year Fund
• Research Field: Genetics/Chromosome dynamics
• Research Institution: Tokyo Institute of Technology
• Principal Investigator: Sato Yuko 東京工業大学, 科学技術創成研究院, 助教 (70435882)
• Project Period (FY): 2018 – 2020
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥14,560,000 (Direct Cost: ¥11,200,000、Indirect Cost: ¥3,360,000)
• Keywords: クロマチン / ZGA / ゼブラフィッシュ / ライブイメージング / HCR / RNA FISH / エピジェネティクス / 胚ゲノム活性化 / 胚性ゲノム活性化 / ヒストン修飾

Outline of Final Research Achievements

Nuclear localization of genes during zebrafish early zygotic genome activation (128-512 cell stages) was investigated using Hybridization chain reaction (HCR). The results showed that relative distances of genes varied in the nucleus, which suggested that each gene localizes randomly in the nucleus during early zygotic genome activation. The living embryos injected with live-cell probes for transcription activation and histone modification were imaged using a SiMView lightsheet fluorescence microscope at Janelia Research Campus (VA, USA). The principal and operation of a diSPIM lightsheet microscope were learned by anticipating the diSPIM workshop held in Marine Biological Laboratory (MA, USA).

Academic Significance and Societal Importance of the Research Achievements

初期発生過程における胚ゲノム活性化メカニズムは、未だ明らかでない部分が多く残されている。細胞核内のDNAはヒストンタンパク質と結合したヌクレオソームを基本単位として、クロマチンと呼ばれる高次構造を形成して機能的に収納されている。近年のクロマチン研究において、初期発生過程の転写活性化とクロマチン高次構造の制御に注目が集まっている。本研究成果は当該分野の新たな知見として重要な意義を持つことが期待される。

Research Products

• [Int'l Joint Research] ニューヨーク大学(アメリカ合衆国) (2018)
• [Journal Article] Transcription organizes euchromatin via microphase separation (2021)
  • Author(s): Hilbert Lennart、Sato Yuko、Kuznetsova Ksenia、Bianucci Tommaso、Kimura Hiroshi、Julicher Frank、Honigmann Alf、Zaburdaev Vasily、Vastenhouw Nadine L.
  • Journal Title: Nature Communications Volume: 12 Issue: 1 Pages: 1360-1360
  • DOI: 10.1038/s41467-021-21589-3
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] H4K20me1 and H3K27me3 are concurrently loaded onto the inactive X chromosome but dispensable for inducing gene silencing (2021)
  • Author(s): Tjalsma Sjoerd J D、Hori Mayako、Sato Yuko、Bousard Aurelie、Ohi Akito、Raposo Ana Claudia、Roensch Julia、Le Saux Agnes、Nogami Jumpei、Maehara Kazumitsu、Kujirai Tomoya、Handa Tetsuya、Bages‐Arnal Sandra、Ohkawa Yasuyuki、Kurumizaka Hitoshi、da Rocha Simao Teixeira、Zylicz Jan J、Kimura Hiroshi、Heard Edith
  • Journal Title: EMBO reports Volume: 22 Issue: 3
  • DOI: 10.15252/embr.202051989
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Visualizing transcription sites in living cells using a genetically encoded probe specific for the elongating form of RNA polymerase II (2021)
  • Author(s): Uchino Satoshi、Ito Yuma、Sato Yuko、Handa Tetsuya、Ohkawa Yasuyuki、Tokunaga Makio、Kimura Hitoshi
  • Journal Title: bioRxiv Volume: - Pages: 441582-441582
  • DOI: 10.1101/2021.04.27.441582
  • Open Access
• [Journal Article] Histone H3K27 acetylation precedes active transcription during zebrafish zygotic genome activation as revealed by live cell analysis. (2019)
  • Author(s): Sato Y, Hilbert L, Oda H, Wan Y, Heddleston JM, Chew T-L, Zaburdaev V, Keller P, Lionnet T, Vastenhouw N, and Kimura H
  • Journal Title: Development Volume: 146 Issue: 19
  • DOI: 10.1242/dev.179127
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Quantitative measurements of chromatin modification dynamics during zygotic genome activation (2019)
  • Author(s): Yuko Sato, Lennart Hilbert, Haruka Oda, Yinan Wan, John M. Heddleston, Teng-Leong Chew, Vasily Zaburdaev, Philipp Keller, Timothee Lionnet, Nadine Vastenhouw, Hiroshi Kimura
  • Journal Title: bioRxiv Volume: 601393 Pages: 601393-601393
  • DOI: 10.1101/601393
  • Open Access / Int'l Joint Research
• [Presentation] Spatiotemporal dynamics of histone modifications during zebrafish zygotic genome activation (2020)
  • Author(s): 佐藤優子、Nguyen Thi Bich Ngoc、小田春佳、半田哲也、西原秀典、原田哲仁、大川恭行、木村宏
  • Organizer: 第43回日本分子生物学会年会
  • Invited
• [Presentation] Histone modification dynamics during zygotic genome activation (2019)
  • Author(s): Yuko Sato, Lennart Hilbert, Vasily Zaburdaev, Nadine Vastenhouw, and Hiroshi Kimura
  • Organizer: International Symposium for Female Researchers in Chromatin Biology 2019
  • Int'l Joint Research