| Outline of Final Research Achievements |
We identified a new white adipose depot with high beiging capacity after cold exposure that surpluses that in other white adipose tissue depots, as assessed by cold-induced gene expression of UCP1, a brown adipose tissue (BAT)-specific marker. Therefore, we coined as iBAT (inducible BAT). From our RNA-seq analysis, we found that the expressions of 35 genes (CIG1-35; cold-induced, iBAT- specific gene 1-35) were significantly increased in iBAT compared with inguinal WAT. Interestingly, CIG1, the most robustly increased gene, was found to be expressed in UCP1-positive cells in iBAT after cold exposure. In addition, we found that CIG1 expression was temporarily increased during differentiation of induced pluripotent stem cells (iPS) cells into brown adipocytes. These results suggest that CIG1 is suggested to be involved not only in early stage of adipocyte differentiation but also in beiging of iBAT under cold exposure.
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