| Outline of Final Research Achievements |
In this study in order to understand the native ultrastructure of enteric glial cells with novel three-dimensional electron microscopy, such as the Focused ion beam / Scanning electron microscopy (FIB/SEM), a snap freezing method of liquid nitrogen jets, combined with very high pressures (cryo-fixation), was analyzed. At first, with using the mouse cortex tissues, the difference among the cryo-fixation and conventional chemical-fixations was confirmed. As a result, dendritic spines showed significantly thinner necks with cryo-fixation. Ｔhis work highlights the usefulness of cryo-fixation for obtaining a more native and detailed view of the architecture of cells and tissues.
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