| Outline of Final Research Achievements |
This study focused on the single cell heterogeneity in cell population that arise Drug Tolerant Persister cells (DTPs), and tried to predict the emergence of DTPs by measuring the cellular intrinsic factor, such as protein expression and localization, and extrinsic factor. Using immunofluorescent imaging, dozens of proteins and molecules were visualized in same cells, and quantitatively analyzed. We are now trying to predict the cellular responses, but using this study, we have successfully constructed a workflow to obtain spatial information of dozen of molecules from a single cell.
|
