| Budget Amount *help |
¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2019: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2018: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2017: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
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| Outline of Final Research Achievements |
The endoplasmic reticulum forms membrane contact sites (MCSs) with other organelles or the plasma membrane. At the MCSs, lipid transfer proteins (LTPs) mediate transport of lipids between membranes. However, the underlying mechanism is incompletely understood. The goal of this study was to investigate the LTP-mediated lipid transport quantitively by taking advantage of the in vitro lipid transfer reactions between liposomes. We established the lipid transfer assay system, in which the rate of lipid transfer can be quantitively evaluated in vitro. A series of in vitro lipid transfer experiments revealed the kinetics of lipid transport between liposomes by oxysterol-binding proteins.
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