| Budget Amount *help |
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2019: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
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| Outline of Final Research Achievements |
This study has developed a molecular tool to manipulate hormone responses in a cell type-specific manner. We focused on auxin, a plant hormone known to be involved in various phenomena in various parts of the plant body. We created artificial pairs of artificial hormones and artificial receptors by simultaneously modifying the auxin molecule and its receptor. Using the artificial pairs, it became possible to precisely manipulate the auxin response in a cell type-specific manner. Also, we applied the artificial pairs to the auxin degron (AID) method, which is a technique to eliminate only the targeted proteins in animal cells by exogenously adding auxin. We succeeded in developing a method to eliminate proteins without cytotoxicity by adding the compounds at concentrations 1000 times lower than those of the conventional method.
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