Analysis of plant growth through the creation of freehand manipulation of hormone responses
| Project/Area Number |
17KT0017
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Multi-year Fund |
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| Section | 特設分野 |
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| Research Field |
Constructive Systems Biology
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| Research Institution | Nagoya University |
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Principal Investigator |
Uchida Naoyuki 名古屋大学, 遺伝子実験施設, 教授 (40467692)
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| Project Period (FY) |
2017-07-18 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2019: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
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| Keywords | オーキシン / 受容体 / 人工ペア / 植物ホルモン / 環境適応 |
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| Outline of Final Research Achievements |
This study has developed a molecular tool to manipulate hormone responses in a cell type-specific manner. We focused on auxin, a plant hormone known to be involved in various phenomena in various parts of the plant body. We created artificial pairs of artificial hormones and artificial receptors by simultaneously modifying the auxin molecule and its receptor. Using the artificial pairs, it became possible to precisely manipulate the auxin response in a cell type-specific manner. Also, we applied the artificial pairs to the auxin degron (AID) method, which is a technique to eliminate only the targeted proteins in animal cells by exogenously adding auxin. We succeeded in developing a method to eliminate proteins without cytotoxicity by adding the compounds at concentrations 1000 times lower than those of the conventional method.
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| Academic Significance and Societal Importance of the Research Achievements |
今回、人工化合物の作成と受容体の改変により創生に成功したオーキシン応答の操作技術は、今後は基礎植物科学における未解明課題の解決に加え、オーキシン作用を作物栽培に応用する展開においても大きく活用されていくと期待される。また，植物で働く低分子植物ホルモンは他にも多数存在するが、オーキシンと同様にその自在応答操作が困難なものは多い。今後、今回の成果をきっかけに、同様の手法を用いた様々な植物ホルモンの応答操作が生み出されていくことも期待できる。
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Report
(5 results)
Research Products
(25 results)
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[Journal Article] A peptide pair coordinates regular ovule initiation patterns with seed number and fruit size.2020
Author(s)
Kawamoto N, Pino Del Carpio D, Hofmann A, Mizuta Y, Kurihara D, Higashiyama T, Uchida N, Torii KU, Colombo L, Groth G, Simon R
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Journal Title
Curr. Biol.
Volume: 30
Issue: 22
Pages: 4352-4361
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] A super strong engineered auxin-TIR1 pair.2018
Author(s)
Yamada R, Murai K, Uchida N, Takahashi K, Iwasaki R, Tada Y, Kinoshita T, Itami K, Torii KU and Hagihara S
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Journal Title
Plant Cell Physiol.
Volume: 59
Issue: 8
Pages: 1538-1544
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Chemical hijacking of auxin signaling with an engineered auxin-TIR1 pair2018
Author(s)
Uchida N, Takahashi K, Iwasaki R, Yamada R, Yoshimura M, Endo TA, Kimura S, Zhang H, Nomoto M, Tada Y, Kinoshita T, Itami K, Hagihara S and Torii KU
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Journal Title
Nature Chem. Biol.
Volume: 14
Issue: 3
Pages: 299-305
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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