Searching for core-network of host factors regulating virus infections by comparing vertebrate and arthropod responses

• Project/Area Number: 17KT0045
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Multi-year Fund
• Section: 特設分野
• Research Field: Complex Systems Disease Theory
• Research Institution: Hokkaido University
• Principal Investigator: Matsuno Keita 北海道大学, 人獣共通感染症国際共同研究所, 講師 (40753306)
• Co-Investigator(Kenkyū-buntansha): 山崎 剛士 北海道大学, 遺伝子病制御研究所, 客員研究員 (70709881)
• Project Period (FY): 2017-07-18 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000); Fiscal Year 2020: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2019: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000); Fiscal Year 2018: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2017: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
• Keywords: アルボウイルス / 宿主因子 / 宿主応答 / RNAウイルス / 病原性 / ネットワーク解析 / 微生物 / ネットワーク / シグナル伝達 / ウィルス

Outline of Final Research Achievements

Infectious diseases caused by arboviruses (viruses transmitted by blood-sucking arthropods and can also grow in the arthropods), such as dengue fever and SFTS, have become a severe problem on public health worldwide. A common feature of arboviruses is that they can grow in both vertebrates and arthropods. In this study, we compared host factor networks between vertebrates and arthropods, and extracted a homologous core network. We then identified several host factors that are likely to be associated with arbovirus replication in cells.

Academic Significance and Societal Importance of the Research Achievements

アルボウイルス（蚊やマダニなどの吸血性節足動物によって媒介されるウイルスのうち、節足動物体内でも増殖するウイルス）は、吸血に乗じて感染を繰り返すために、脊椎動物と節足動物という大きくことなる環境を往復することに特化しているウイルスである。そのため、他のウイルスと異なり、脊椎動物と節足動物の両方に保存されている因子を利用して増殖できると考えられる。本研究では、そうした因子を明らかにし、アルボウイルス感染症に対する治療薬の開発などに役立つ成果を得た。

Research Products

• [Int'l Joint Research] Mayo clinic(米国)
• [Int'l Joint Research] メイヨークリニック(米国)
• [Int'l Joint Research] テキサス大学(米国)
• [Journal Article] Infection of newly identified phleboviruses in ticks and wild animals in Hokkaido, Japan indicating tick-borne life cycles (2019)
  • Author(s): Torii Shiho、Matsuno Keita、Qiu Yongjin、Mori-Kajihara Akina、Kajihara Masahiro、Nakao Ryo、Nao Naganori、Okazaki Katsunori、Sashika Mariko、Hiono Takahiro、Okamatsu Masatoshi、Sakoda Yoshihiro、Ebihara Hideki、Takada Ayato、Sawa Hirofumi
  • Journal Title: Ticks and Tick-borne Diseases Volume: 10 Issue: 2 Pages: 328-335
  • DOI: 10.1016/j.ttbdis.2018.11.012
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Fatal Tickborne Phlebovirus Infection in Captive Cheetahs, Japan (2018)
  • Author(s): Matsuno Keita、Nonoue Noriyuki、Noda Ayako、Kasajima Nodoka、Noguchi Keita、Takano Ai、Shimoda Hiroshi、Orba Yasuko、Muramatsu Mieko、Sakoda Yoshihiro、Takada Ayato、Minami Shinji、Une Yumi、Morikawa Shigeru、Maeda Ken
  • Journal Title: Emerging Infectious Diseases Volume: 24 Issue: 9 Pages: 1726-1729
  • DOI: 10.3201/eid2409.171667
  • NAID: 120006529645
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] The Unique Phylogenetic Position of a Novel Tick-Borne Phlebovirus Ensures an Ixodid Origin of the Genus Phlebovirus (2018)
  • Author(s): Matsuno Keita、Kajihara Masahiro、Nakao Ryo、Nao Naganori、Mori-Kajihara Akina、Muramatsu Mieko、Qiu Yongjin、Torii Shiho、Igarashi Manabu、Kasajima Nodoka、Mizuma Keita、Yoshii Kentaro、Sawa Hirofumi、Sugimoto Chihiro、Takada Ayato、Ebihara Hideki
  • Journal Title: mSphere Volume: 3 Issue: 3
  • DOI: 10.1128/msphere.00239-18
  • NAID: 120006501204
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] 重症熱性血小板減少症候群ウイルスの核タンパク質と宿主RNA間の相互作用がウイルス増殖に与える新たな機能の解明 (2019)
  • Author(s): 水間奎太, 松野啓太, 岡松正敏, 高田礼人, 迫田義博
  • Organizer: 第16回 ウイルス学キャンプ in 湯河原
• [Presentation] Profiling RNAs binding with the nucleoprotein of severe fever with thrombocytopenia syndrome virus (SFTSV) revealed orchestrated replication of a segmented RNA virus (2019)
  • Author(s): Keita Mizuma, Keita Matsuno, Masatoshi Okamatsu, Ayato Takada, Yoshihiro Sakoda
  • Organizer: International meeting on arboviruses and their vectors (IMAV 2019)
  • Int'l Joint Research
• [Presentation] マダニ中の多様なフレボウイルスは何者か？ (2019)
  • Author(s): 松野 啓太、中尾 亮、草木迫 浩大、大場 靖子、澤 洋文
  • Organizer: 第162回 日本獣医学会学術集会
• [Presentation] 重症熱性血小板減少症候群ウイルスの核タンパク質Nと結合する宿主RNAの機能解明 (2019)
  • Author(s): 水間奎太, 松野啓太, 岡松正敏, 高田礼人, 迫田義博
  • Organizer: 第162回 日本獣医学会学術集会
• [Presentation] マダニ中のフレボウイルスの遺伝子系統解析に基づく性状推定 (2019)
  • Author(s): 松野啓太、中尾亮、梶原将大、下田宙、海老原秀喜、高田礼人、前田健、岡松正敏、迫田義博
  • Organizer: 第162回 日本獣医学会学術集会
• [Presentation] 重症熱性血小板減少症候群ウイルスの核タンパク質Nと結合する宿主RNAの機能解明 (2019)
  • Author(s): 水間 奎太、松野 啓太、岡松 正敏、高田 礼人、迫田 義博
  • Organizer: 第67回日本ウイルス学会学術集会
• [Presentation] Complete mitochondrial genome analysis of ticks (2018)
  • Author(s): Ryo Nakao, Gohta Kinoshita, Ayumi Saito, Yongjin Qiu, Keita Matsuno, Chihiro Sugimoto, Ken Katakura
  • Organizer: 14th International Congress of Parasitology (ICOPA 2018)
  • Int'l Joint Research
• [Presentation] In-depth analysis of viral and host RNAs interacting with severe fever with thrombocytopenia syndrome virus (SFTSV) nucleoprotein N (2018)
  • Author(s): Keita Mizuma, Keita Matsuno, Masatoshi Okamatsu, Ayato Takada, Yoshihiro Sakoda
  • Organizer: 日本ウイルス学会北海道支部 平成30年度 夏季シンポジウム
• [Presentation] Genetic diversity among phleboviruses identified in ticks; implication of distinct evolutional pathways of phleboviruses (2017)
  • Author(s): Keita Matsuno, Masahiro Kajihara, Nodoka Kasajima, Ryo Nakao, Shiho Torii, Hiroshi Shimoda, Ken Maeda, Ayato Takada, Hideki Ebihara, Hirofumi Sawa
  • Organizer: 第16回あわじしま感染症・免疫フォーラム
  • Int'l Joint Research / Invited
• [Presentation] Suppression of PKR signaling pathway by nonstructural protein of severe fever with thrombocytopenia syndrome virus. (2017)
  • Author(s): Kasajima N, Matsuno K, Torii S, Hiono T, Okamatsu M, Ebihara H, Sakoda Y
  • Organizer: 第66回日本ウイルス学会学術集会
  • Int'l Joint Research