| Budget Amount *help |
¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2019: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2018: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2017: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
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| Outline of Final Research Achievements |
I studied to develop an inclusive and high throughput analysis method for tRNA modifications in urine and peripheral blood samples, and applied the method to diagnose mitochondrial disease and Asian-type diabetes. I succeeded to develop a analysis method by which we could detect more than 30 modified nucleosides within 1 h by LC/MS after removal of proteins form a small volume of urine and peripheral blood. I verified that we diagnosed mitochondrial disease by measuring two kinds of modified nucleosides in urine using the new analysis method. Moreover, this method was also useful for the diagnosis of Asian-type diabetes in patients who carry risk alleles in CDKAL1 gene.
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