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In-vitro evolution of endosymbiosis between an alga and a protozoan

Research Project

Project/Area Number 17KT0111
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section特設分野
Research Field Constructive Systems Biology
Research InstitutionEhime University

Principal Investigator

Nakajima Toshiyuki  愛媛大学, 理工学研究科(理学系), 教授 (70314945)

Project Period (FY) 2017-07-18 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords共生の進化 / 実験進化 / 藻類 / 大腸菌 / 原生動物 / 細胞内共生 / 生態系 / クロレラ / テトラヒメナ / 繊毛虫 / マイクロコズム / 進化
Outline of Final Research Achievements

Using a 13-year culture of an experimental ecosystem composed of three species (an alga, a bacterium, a ciliate), the evolutionary process of how an endosymbiotic association can evolve between unassociated alga and ciliate. Specifically, we tested the following, our original hypothesis using isolates of both species in the culture. “Endosymbiotic associations evolve between heterotrophic and photo-trophic organisms under resource-limited conditions produced in a mature-stage ecosystem, by which they raise exchanges of essential resources (quantity and quality) and reduce the dependency on the external sources such as bacteria for them.” The results indicated that the ciliate, originally survived/reproduced by consuming the bacterium, received essential resources of amino acids, vitamins, nucleotides, and sugars and became less dependent on bacterial cells. These results support the above hypothesis.

Academic Significance and Societal Importance of the Research Achievements

本研究は、13年に及ぶ実験生態系の長期培養系を解析し,従属栄養生物(繊毛虫)と光栄養生物(藻類)との間の細胞内共生の進化を実証的に解析したものであり,このような解析は国内外で他に例がない.従来の研究が共生をすでに進化させた生物を用いた解析であるのに対し,本研究ではモデル生態系の中で共生関係にない種間に共生が進化する過程を追跡するものである.“光照射下で自律的に存続する実験生態系モデル”は,そのツールとして有効であることを示した.このモデルは、進化を生態系過程との関わりにおいて理解する重要性の提起に加え,それを解析するツールとして他の研究者に影響を与えると考えられる.

Report

(5 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (11 results)

All 2021 2020 2019 2018 2017

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 2 results) Presentation (7 results) Book (1 results)

  • [Journal Article] Symbiogenesis is driven through hierarchical reorganization of an ecosystem under closed or semi-closed conditions. BioSystems, 2052021

    • Author(s)
      Toshiyuki Nakajima
    • Journal Title

      Biosystems

      Volume: 205 Pages: 104427-104427

    • DOI

      10.1016/j.biosystems.2021.104427

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Unification of Epistemic and Ontic Concepts of Information, Probability, and Entropy, Using Cognizers-System Model2019

    • Author(s)
      Toshiyuki Nakajima
    • Journal Title

      Entropy

      Volume: - Issue: 2 Pages: 1-26

    • DOI

      10.3390/e21020216

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Ecological extension of the theory of evolution by natural selection from a perspective of Western and Eastern holistic philosophy2017

    • Author(s)
      Toshiyuki Nakajima
    • Journal Title

      Progress in Biophysics and Molecular Biology

      Volume: 131 Pages: 298-311

    • DOI

      10.1016/j.pbiomolbio.2017.09.005

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 合成生態系を用いた藻類-細菌の集塊形成による共生の進化2021

    • Author(s)
      安部雄一,藤井陽介、堀澤栄,佐久間洋,中島敏幸
    • Organizer
      日本ゲノム微生物学会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 合成生態系を用いた藻類-細菌の集塊形成による共生の進化2020

    • Author(s)
      安部雄一,藤井陽介、堀澤栄,佐久間洋,中島敏幸
    • Organizer
      日本ゲノム微生物学会
    • Related Report
      2019 Research-status Report
  • [Presentation] 緑藻・大腸菌・テトラヒメナの3種からなる実験生態系の長期培養における緑藻-テトラヒメナの細胞内共生の進化と生態系過程2019

    • Author(s)
      中島敏幸,佐野明子,松原俊行,藤川佳之,大西陽一郎,松田達也
    • Organizer
      共生起源研究会
    • Related Report
      2019 Research-status Report
  • [Presentation] 合成生態系における藻類・細菌間の種間関係の進化と大腸菌の全ゲノム解析2019

    • Author(s)
      安部雄一,坪井睦枝,松浦正幸,藤川佳之、松本沙千,堀澤栄,佐久間洋, 中島敏幸
    • Organizer
      日本ゲノム微生物学界
    • Related Report
      2018 Research-status Report
  • [Presentation] 藻類・細菌・繊毛虫からなる合成生態系を用いた進化の解析:大腸菌の進化と種間関係2019

    • Author(s)
      中島敏幸,安部雄一,坪井睦枝,松浦正幸,松本沙千、堀澤栄,佐久間洋
    • Organizer
      日本ゲノム微生物学界
    • Related Report
      2018 Research-status Report
  • [Presentation] 長期培養モデル生態系における藻類・細菌間共生の進化2018

    • Author(s)
      安部雄一, 藤井陽介, 松浦正幸, 坪井睦枝, 松本沙千, 堀澤栄,佐久間洋,中島敏幸
    • Organizer
      日本原生生物学会
    • Related Report
      2018 Research-status Report
  • [Presentation] 緑藻-繊毛虫の細胞内共生の進化における共生系内の資源交換と系外から取り込む資源の変化:3種モデル生態系を用いた解析2018

    • Author(s)
      松田達也,佐古壮一郎,西窪健太,中島敏幸
    • Organizer
      日本原生生物学会
    • Related Report
      2018 Research-status Report
  • [Book] 遺伝子・多様性・循環の科学: 生態学の領域融合へ 10章:生態系とダーウィンマシーン2019

    • Author(s)
      中島敏幸(門脇・立木編)
    • Total Pages
      432
    • Publisher
      京都大学学術出版会
    • ISBN
      4814001908
    • Related Report
      2018 Research-status Report

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Published: 2017-07-21   Modified: 2022-12-28  

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