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Elucidation of cell movement independent morphogenetic mechanisms in notochord formation

Research Project

Project/Area Number 17KT0114
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section特設分野
Research Field Constructive Systems Biology
Research InstitutionInstitute of Physical and Chemical Research (2018-2019)
Okinawa Institute of Science and Technology Graduate University (2017)

Principal Investigator

Yasuoka Yuuri  国立研究開発法人理化学研究所, 生命医科学研究センター, 研究員 (70724954)

Project Period (FY) 2017-07-18 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords進化発生 / 収斂伸長運動 / 液胞 / 細胞外基質 / ケラタン硫酸 / ナメクジウオ / ツメガエル / ゲノム編集 / 発生 / 細胞膜輸送 / 進化 / 数理モデル
Outline of Final Research Achievements

Notochord is defined as a vacuolated supporting organ of larval tail, which characterizes chordates. Here I proposed a self-organizing morphogenetic mechanism forming the notochord rod controlled by vacuolation, membrane trafficking, extracellular matrix formation, and cell proliferation; named "turgor pressure-sheath strength model." As a part of this model, I examined keratan sulfate biosynthesis in Xenopus embryos. Among carbohydrate sulfotransferase genes, chst3 and chst6 were identified as notochord-enriched genes. Loss of function analysis demonstrated that they were required for keratan sulfate synthesis in the notochord.
To examine evolutionary conservation of notochord components, I investigated expression patterns of notochord related genes in amphioxus embryos by in situ hybridization. The result suggests that caveolin, calumenin, leprecan, and so on have functioned as ancestral notochord components since chordate arose.

Academic Significance and Societal Importance of the Research Achievements

【学術的意義】脊索は脊椎動物を含む脊索動物を特徴づける器官だが、その細胞生物学的な性質を俯瞰的にとらえた研究は少ない。本研究で提唱した「液胞膨圧ー脊索鞘強度平衡モデル」は、これまでの知見を合理的にまとめた優れたモデルであり、今後その詳細な分子メカニズムの解明が期待される。また、ナメクジウオの遺伝子発現パターンを明らかにし、祖先的な脊索構成要素の一部を明らかにできたのも大変意義深い。
【社会的意義】脊索遺残組織は椎間板髄鞘としてヒト成体にも存在し、その損傷は腰痛などの原因となる。また、椎間板髄鞘から生じる腫瘍(脊索腫)が希少疾患として知られている。本研究はこれらの疾患研究の基礎となりうる。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (25 results)

All 2020 2019 2018 2017 Other

All Journal Article (9 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 9 results,  Open Access: 5 results) Presentation (15 results) (of which Int'l Joint Research: 2 results,  Invited: 1 results) Remarks (1 results)

  • [Journal Article] Morphogenetic mechanisms forming the notochord rod: The turgor pressure‐sheath strength model2020

    • Author(s)
      Yasuoka Yuuri
    • Journal Title

      Development, Growth & Differentiation

      Volume: 00 Issue: 6 Pages: 1-12

    • DOI

      10.1111/dgd.12665

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Evolution of cis-regulatory modules for the head organizer gene goosecoid in chordates: comparisons between Branchiostoma and Xenopus2019

    • Author(s)
      Yasuoka Yuuri、Tando Yukiko、Kubokawa Kaoru、Taira Masanori
    • Journal Title

      Zoological Letters

      Volume: 5 Issue: 1 Pages: 1-17

    • DOI

      10.1186/s40851-019-0143-1

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Evolutionary History of GLIS Genes Illuminates Their Roles in Cell Reprograming and Ciliogenesis2019

    • Author(s)
      Yasuoka Yuuri、Matsumoto Masahito、Yagi Ken、Okazaki Yasushi
    • Journal Title

      Molecular Biology and Evolution

      Volume: 37 Issue: 1 Pages: 100-109

    • DOI

      10.1093/molbev/msz205

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] De novo transcription of multiple Hox cluster genes takes place simultaneously in early Xenopus tropicalis embryos2019

    • Author(s)
      Kondo Mariko、Matsuo Megumi、Igarashi Kento、Haramoto Yoshikazu、Yamamoto Takayoshi、Yasuoka Yuuri、Taira Masanori
    • Journal Title

      Biology Open

      Volume: 8

    • DOI

      10.1242/bio.038422

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Microinjection of DNA Constructs into Xenopus Embryos for Gene Misexpression and cis-Regulatory Module Analysis2018

    • Author(s)
      Yasuoka Yuuri、Taira Masanori
    • Journal Title

      Cold Spring Harbor Protocols

      Volume: 2019 Issue: 1 Pages: pdb.prot097279-pdb.prot097279

    • DOI

      10.1101/pdb.prot097279

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] The Molecular Basis of the Gastrula Organizer in Amphibians and Cnidarians2018

    • Author(s)
      Yasuoka Yuuri、Taira Masanori
    • Journal Title

      Reproductive and Developmental Strategies. Diversity and Commonality in Animals (Book)

      Volume: - Pages: 667-708

    • DOI

      10.1007/978-4-431-56609-0_31

    • ISBN
      9784431566076, 9784431566090
    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Phosphorylation states change Otx2 activity for cell proliferation and patterning in the Xenopus embryo2018

    • Author(s)
      Satou Yumeko、Minami Kohei、Hosono Erina、Okada Hajime、Yasuoka Yuuri、Shibano Takashi、Tanaka Toshiaki、Taira Masanori
    • Journal Title

      Development

      Volume: 145 Issue: 5 Pages: 159640-159640

    • DOI

      10.1242/dev.159640

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] High variability of expression profiles of homeologous genes for Wnt, Hh, Notch, and Hippo signaling pathways in Xenopus laevis.2017

    • Author(s)
      Michiue T, Yamamoto T, Yasuoka Y, Goto T, Nakayama T, Nagura K, Ikeda T, Taira M, Kinoshita T
    • Journal Title

      Dev. Biol.

      Volume: 印刷中 Issue: 2 Pages: 270-290

    • DOI

      10.1016/j.ydbio.2016.12.006

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Conservatism and variability of gene expression profiles among homeologous transcription factors in Xenopus laevis.2017

    • Author(s)
      Watanabe, M., Yasuoka, Y., Mawaribuchi, S., Kuretani, A., Ito, M., Kondo, M., Ochi, H., Ogino, H., Fukui, A., Taira, M. and Kinoshita, T.
    • Journal Title

      Dev. Biol.

      Volume: 426 Issue: 2 Pages: 301-324

    • DOI

      10.1016/j.ydbio.2016.09.017

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Genome-wide Measurement for Fluctuation of Gene Regulatory Networks in Xenopus embryos2019

    • Author(s)
      Yasuoka Yuuri
    • Organizer
      Xenopus Resources and Emerging Technologies Meeting
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Why was the notail gene lost in many vertebrate lineages?2019

    • Author(s)
      Yasuoka Yuuri, Satoh Noriyuki
    • Organizer
      52nd Annual Meeting of JSDB
    • Related Report
      2019 Annual Research Report
  • [Presentation] Evolutionary History of GLIS Genes Illustrates their Fundamental Roles in Cell Reprogramming and Ciliogenesis2019

    • Author(s)
      Yasuoka Yuuri, Matsumoto Masayuki, Yagi Ken, Okazaki Yasushi
    • Organizer
      21st Annual Meeting of the Society of Evolutionary Studies, Japan
    • Related Report
      2019 Annual Research Report
  • [Presentation] Fluctuation of GRN in Xenopus tropicalis embryos2019

    • Author(s)
      Yasuoka Yuuri
    • Organizer
      12th Japan Xenopus Conference
    • Related Report
      2019 Annual Research Report
  • [Presentation] Complex evolution of brachyury paralogs during vertebrate notochord development2018

    • Author(s)
      Yuuri Yasuoka and Noriyuki Satoh
    • Organizer
      第46回内藤コンファレンス
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] 脊椎動物の脊索発生におけるBrachyuryパラログの複雑な進化2018

    • Author(s)
      安岡有理、佐藤矩行
    • Organizer
      第20回日本進化学会年会
    • Related Report
      2018 Research-status Report
  • [Presentation] 進化学の視点がリプログラミング研究にどう貢献できるか?2018

    • Author(s)
      安岡有理
    • Organizer
      第10回EvoDevo青年の会
    • Related Report
      2018 Research-status Report
  • [Presentation] ネッタイツメガエル胚発生における転写因子ー標的遺伝子関係の揺らぎ測定2018

    • Author(s)
      安岡有理
    • Organizer
      第12回XCIJ首都圏支部会
    • Related Report
      2018 Research-status Report
  • [Presentation] Keratan sulfate produces “water bags” in embryos2018

    • Author(s)
      Yuuri Yasuoka
    • Organizer
      第51回日本発生生物学会年会
    • Related Report
      2018 Research-status Report
  • [Presentation] Xenopus胚でCRISPRi (dCas9)は有効か?2017

    • Author(s)
      安岡有理
    • Organizer
      第3回次世代両生類研究会
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] Independent subfunctionalization of brachyury paralogs in vertebrate lineages2017

    • Author(s)
      Yuuri Yasuoka and Noriyuki Satoh
    • Organizer
      ConBio2017
    • Related Report
      2017 Research-status Report
  • [Presentation] Independent subfunctionalization of brachyury paralogs in vertebrate lineages2017

    • Author(s)
      Yuuri Yasuoka and Noriyuki Satoh
    • Organizer
      The 29th CDB meeting
    • Related Report
      2017 Research-status Report
  • [Presentation] 細胞運動非依存的な脊索の自律的形態形成メカニズムを探る2017

    • Author(s)
      安岡有理
    • Organizer
      第10回日本ツメガエル研究集会
    • Related Report
      2017 Research-status Report
  • [Presentation] 年に一度のサンゴ胚を用いた遺伝子機能解析2017

    • Author(s)
      安岡有理
    • Organizer
      第3回ユニークな少数派実験動物を扱う若手が最先端アプローチを勉強する会
    • Related Report
      2017 Research-status Report
  • [Presentation] Independent subfunctionalization of brachyury paralogs in vertebrate lineages2017

    • Author(s)
      Yuuri Yasuoka and Noriyuki Satoh
    • Organizer
      第50回日本発生生物学会年会
    • Related Report
      2017 Research-status Report
  • [Remarks] 初期化因子の驚きの進化を解明 -リプログラミング研究に新たな視点を与える-

    • URL

      https://www.riken.jp/press/2019/20190913_3/index.html

    • Related Report
      2019 Annual Research Report

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Published: 2017-07-21   Modified: 2021-02-19  

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