| Budget Amount *help |
¥431,210,000 (Direct Cost: ¥331,700,000、Indirect Cost: ¥99,510,000)
Fiscal Year 2010: ¥79,430,000 (Direct Cost: ¥61,100,000、Indirect Cost: ¥18,330,000)
Fiscal Year 2009: ¥79,430,000 (Direct Cost: ¥61,100,000、Indirect Cost: ¥18,330,000)
Fiscal Year 2008: ¥79,430,000 (Direct Cost: ¥61,100,000、Indirect Cost: ¥18,330,000)
Fiscal Year 2007: ¥79,430,000 (Direct Cost: ¥61,100,000、Indirect Cost: ¥18,330,000)
Fiscal Year 2006: ¥113,490,000 (Direct Cost: ¥87,300,000、Indirect Cost: ¥26,190,000)
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| Research Abstract |
We developed man-made tools (super artificial restriction enzyme) which cut double-stranded DNA at desired site with desired site-specificity. Even huge genomic DNA (e.g., the genome of human beings composed of 3x10^9 base-pairs) was selectively hydrolyzed at one target site. The scission fragments were connected with foreign DNA using DNA ligase, and the recombinant DNA was successfully expressed in mammalian cells. Furthermore, the site-selective scissions by the super artificial restriction enzyme were satisfactorily recognized by the repair system in human cells and promoted the desired homologous recombination. High potential of the super artificial restriction enzyme for future applications has been indicated.
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