Development of Metabolomics Technology and Its Application to the Prediction of Novel Metabolic Pathways
| Project/Area Number |
18016028
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| Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | Keio University |
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Principal Investigator |
TOMITA Masaru Keio University, 環境情報学部, 教授 (60227626)
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| Co-Investigator(Kenkyū-buntansha) |
NISHIOKA Takaaki 慶應義塾大学, 政策・メディア研究科, 教授 (80026559)
SOGA Tomoyoshi 慶應義塾大学, 環境情報学部, 教授 (60338217)
KANAI Akio 慶應義塾大学, 環境情報学部, 教授 (60260329)
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| Project Period (FY) |
2006 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥47,700,000 (Direct Cost: ¥47,700,000)
Fiscal Year 2009: ¥12,100,000 (Direct Cost: ¥12,100,000)
Fiscal Year 2008: ¥12,000,000 (Direct Cost: ¥12,000,000)
Fiscal Year 2007: ¥11,900,000 (Direct Cost: ¥11,900,000)
Fiscal Year 2006: ¥11,700,000 (Direct Cost: ¥11,700,000)
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| Keywords | メタボローム解析 / バイオインフォマティクス / 質量分析 / 代謝流束解析 / ケモインフォマティクス / MassBank / pH制御合成法 / プロテオーム解析 / emPAI法 / システムバイオロジー / メタボローム / 質量分析計 / キャピラリー電気泳動-質量分析計 / E-Cell / 赤血球 / 解糖系 / 大腸菌 / エネルギー代謝 / 頑強性 |
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| Research Abstract |
We have attained highly reliable identification and highly sensitive quantification in metabolomics based on capillary electrophoresis-mass spectrometry (CE-MS) by developing bioinformatics tools for high-throughput data analysis, flux analysis, modeling metabolism, and finding metabolic pathways. Usefulness of the tools developed were confirmed by their successful applications to the studies on integrative omics analysis of Escherichia coli mutant cells, metabolic pathway analyses using metabolic perturbations by photoperiod in Oryza sativa leaves, and molecular mechanism of non-oxidative phosphorylation energy production in cancer cells.
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Report
(6 results)
Research Products
(102 results)
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[Journal Article] In silico modeling and metabolome analysis of long-stored erythrocytes to improve blood storage methods2009
Author(s)
Nishino, T., Yachie, A.K., Hirayama. A., Soga, T., Suematsu, T., Tomita, M.
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Journal Title
J. Biotech 144
Pages: 212-223
Related Report
Peer Reviewed
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[Journal Article] Capillary electrophoresis mass spectrometry-based saliva metabolomics identified oral, breast and pancreatic cancer-specific profiles2009
Author(s)
Sugimoto, M., Wong, D.T., Hirayama, A., Soga, T., Tomita, M.
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Journal Title
Metabolomics 6
Pages: 78-95
Related Report
Peer Reviewed
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[Journal Article] Differential Metabolomics Software for Capillary Electrophoresis-Mass Spectrometry Data Analysis2009
Author(s)
Sugimoto, M., Hirayama, A., Ishikawa, T., Baran, R., Robert, M., Uehara, K., Soga, T., Tomita, M.
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Journal Title
Metabolomics 6
Pages: 27-41
Related Report
Peer Reviewed
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[Journal Article] Correlation between Sensory Evaluation Scores of Japanese Sakeand MetabolomeProfiles2009
Author(s)
Sugimoto, M., Koseki, T., Hirayama, A., Abe, S., Sano, T., Tomita, M., Soga, T.
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Journal Title
J. Agric. Food Chem 58
Pages: 374-383
Related Report
Peer Reviewed
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[Journal Article] Multiple High-Throughput Analyses Monitor the Response of E.coli to Perturbations.2007
Author(s)
Ishii N, Nakahigashi K, Baba T, Robert M, Soga T, Kanai A, Hirasawa T, Naba M, Hirai K, Hoque A, Ho PY, Kakazu Y, Sugawara K, Igarashi S, Harada S, Masuda T, Sugiyama N, Togashi T, Hasegawa M, Takai Y, Yugi K, Arakawa K, Iwata N, Toya Y, Nakayama Y, Nishioka T, Shimizu K, Mori H, Tomita M
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Journal Title
Science 10.112(DOI:10.1126/science.1132067)
Related Report
Peer Reviewed
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[Journal Article] Differential metabolomics reveals ophthalmic acid as an oxidative stress biomarker indicating hepatic glutathione consumption.2006
Author(s)
Soga T, Baran R, Suematsu M, Ueno Y, Ikeda S, Sakurakawa T, Kakazu Y,Ishikawa T, Robert M, Nishioka T, Tomita M
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Journal Title
The Journalofbiologicalchemistry 281(24)
Pages: 16768-16776
Related Report
Peer Reviewed
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