• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

新規テトロドトキシン類縁体の単離と構造、活性、及びフグ体内変換

Research Project

Project/Area Number 18032014
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Review Section Science and Engineering
Research InstitutionTohoku University

Principal Investigator

山下 まり  Tohoku University, 大学院・農学研究科, 教授 (50192430)

Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥6,400,000 (Direct Cost: ¥6,400,000)
Fiscal Year 2007: ¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2006: ¥3,200,000 (Direct Cost: ¥3,200,000)
Keywordsテトロドトキシン / LC / MS / フグ / Na+チャネル
Research Abstract

テトロドトキシン(TTX)は、その強力かつ特異的なNa^+チャネル阻害能と特異な化学構造から生化学、合成化学両面から注目されてきた。最近TTX類の分析に有効なHILIC-ESI-LC/MS条件を確立した。この方法で分析すると、5,6,11-trideoxyTTXはフグ卵巣特異的にTTXと同等程度に多量に存在したので、この類縁体はTTXの生合成または代謝系に関わる可能性が示唆された。さらに、LC/MS分析により、フグ卵巣中に構造未定のdeoxy体の存在が示された。これは、4-epi-5-deoxyTTXであることを、NMRにより明らかにした。また、大阪市立大学大船教授から^<13>Cラベル5,6,11-trideoxyTTXを供与された。本研究では本化合物をフグやイモリに、腹腔内または経口投与して類縁体内で変換があるかどうかを、ESI-LC/MSおよびNMR(HMBC)で追跡した。その結果、既知のTTX類縁体への変換は検出されず、1%以下であることが明らかになり、これらの類縁体は、TTXを生産する起源生物により生産される可能性が示唆された。

Report

(2 results)
  • 2007 Annual Research Report
  • 2006 Annual Research Report
  • Research Products

    (12 results)

All 2008 2007 2006

All Journal Article (8 results) (of which Peer Reviewed: 5 results) Presentation (3 results) Book (1 results)

  • [Journal Article] Two critical residues in p-loop regions of puffer fish Na+ channels on TTX-sensitivity2008

    • Author(s)
      Satoshi Maruta
    • Journal Title

      Toxicon 51

      Pages: 381-387

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Accumulation of tetrodotoxin and 4,9-anhydrotetorodotoxin in cultured juvenile kusafugu Fugu niphobles by dietary administration of natural toxic komonfugu Fugu poecilonotus liver2008

    • Author(s)
      Michiko Kono
    • Journal Title

      Toxicon (accepted)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Polycavernoside C and C2, the new analogs of the human lethal toxin polycavernoside A, from the red alga, Gracilaria edulis2007

    • Author(s)
      Mari Yotsu-Yamashita
    • Journal Title

      Tetrahedron Lett 48

      Pages: 2255-2259

    • Related Report
      2007 Annual Research Report 2006 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Tetrodotoxin and its analogue 6-epitetrodotoxin in newts (Triturus spp.; Urodela, Salamandridae) from southern Germany2007

    • Author(s)
      Mari Yotsu-Yamashita
    • Journal Title

      Toxicon 50

      Pages: 306-309

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 6, 11-Dideoxytetrodotoxin from the puffer fish, Fugu pardalis2007

    • Author(s)
      Jun-Ho Jang
    • Journal Title

      Toxicon 50

      Pages: 947-951

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Tetrodotoxin and its analogue 6-epitetrodotoxin in newts (Triturus spp.; Urodela, Salamandridae) from southern Germany2007

    • Author(s)
      Mari Yotsu-Yamashita
    • Journal Title

      Toxicon (In press)(印刷中)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Distribution of tetrodotoxin in the body of the blue-ringed octopus(Hapalochlaena maculosa)2007

    • Author(s)
      Mari Yotsu-Yamashita
    • Journal Title

      Toxicon 49

      Pages: 410-412

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Distribution of tetrodotoxin, saxitoxin, and their analogs among tissues of the puffer fish Fugu pardalis2006

    • Author(s)
      Junho Jang
    • Journal Title

      Toxicon 48

      Pages: 980-987

    • Related Report
      2006 Annual Research Report
  • [Presentation] アカハライモリとミドリフグに投与した13Cラベル5,6,11-トリデオキシテトロドトキシンの変換反応の検索2008

    • Author(s)
      Jun-Ho Jang
    • Organizer
      2008(平成20)年度日本水産学会春季大会
    • Place of Presentation
      東海大学(静岡市)
    • Year and Date
      2008-03-30
    • Related Report
      2007 Annual Research Report
  • [Presentation] Examination of Transformation of [10-^<13>C]5,6,11-trideoxy-tetrodotoxin administrated to the living newts and puffer fish2008

    • Author(s)
      Jun-Ho Jang
    • Organizer
      グローバルCOE第一回国際シンポジウム
    • Place of Presentation
      東北大学(仙台市)
    • Year and Date
      2008-03-13
    • Related Report
      2007 Annual Research Report
  • [Presentation] 13C ラベル5,6,11-トリデオキシテトロドトキシンを投与したアカハライモリ(Cynops pyrrohogaster)とミドリフグ(Tetraodon nigroviridis)の体内変換反応の検索2007

    • Author(s)
      Jun-Ho Jang
    • Organizer
      第4回東北大学バイオサイエンスシンポジウム
    • Place of Presentation
      東北大学(仙台市)
    • Year and Date
      2007-06-04
    • Related Report
      2007 Annual Research Report
  • [Book] Polycaverncsides, In "Phycotoxins: chemistry and biochemistry"Captor 152007

    • Author(s)
      Leo A. Paquette and Mari Yotsu-Yamashita
    • Total Pages
      345
    • Publisher
      Blackwell Publ
    • Related Report
      2007 Annual Research Report

URL: 

Published: 2006-04-01   Modified: 2018-03-28  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi