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難治性疾患に関わるシグナル伝達分子を標的とした天然機能分子の探索

Research Project

Project/Area Number 18032020
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Review Section Science and Engineering
Research InstitutionChiba University

Principal Investigator

石橋 正己  Chiba University, 大学院・薬学研究院, 教授 (90212927)

Co-Investigator(Kenkyū-buntansha) 荒井 緑  千葉大学, 大学院・薬学研究院, 准教授 (40373261)
大槻 崇  千葉大学, 大学院・薬学研究院, 助教 (30401011)
Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥6,400,000 (Direct Cost: ¥6,400,000)
Fiscal Year 2007: ¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2006: ¥3,200,000 (Direct Cost: ¥3,200,000)
Keywords薬学 / 有機化学 / シグナル伝達 / 癌 / 生理活性 / 天然物 / 探索 / スクリーニング
Research Abstract

当研究室独自に構築した未利用天然物ライブラリーを用いて,主に癌に関連するシグナル伝達分子であるTRAILおよびWntシグナル等を標的とした天然物の探索を行った.
1.デス受容体の一つとしてDR5(=TRAIL-R2)に着目し,タイ産Millettia brandisiana(マメ科)について,DR5誘導作用をもつ活性成分の探索を行った.その結果本植物より新規イソフラボノイドbrandisianin A-Fを含む11種の化合物を単離した.これらのうち,brandisianin Dが17.5μMでコントロールに比べて2.4倍のDR5プロモーター活性を上昇させることが判明した.またTRAIL耐性AGS細胞に対してTRAILとbrandisianin Dの併用処理により有意に細胞生存率が減少させた.この結果から,brandisianin DはDR5の発現誘導を介してTRAIL感受性を増強させたものと示唆された.
2.Wntシグナル伝達経路における転写因子TCFの転写活性化を抑制する天然物に着目し,タイ産Eleutherine palmifolia(アヤメ科)について活性成分の探索を行った.TCF転写阻害活性は,TCF結合領域を有するルシフェラーゼレポータープラスミドを安定導入した293T/STF細胞株によるアッセイシステムを用いた.本植物のメタノール抽出物について,活性試験を指標とした分画を行い,10種の化合物を単離した.これらの化合物のうち3種は新規ナフトキノン配糖体であり,Eleutherinoside A,B,及びCと命名しそれぞれ構造を決定した.これらの化合物は顕著なTCF転写阻害活性を有していた.

Report

(2 results)
  • 2007 Annual Research Report
  • 2006 Annual Research Report
  • Research Products

    (16 results)

All 2008 2007 2006 Other

All Journal Article (9 results) (of which Peer Reviewed: 3 results) Presentation (1 results) Book (2 results) Remarks (1 results) Patent(Industrial Property Rights) (3 results)

  • [Journal Article] Brandisianins A-F, Isoflavonoids Isolated from Millettia brandisiana in a Screening Program for Death-Receptor Expression Enhancement Activity2007

    • Author(s)
      H. Kikuchi
    • Journal Title

      Journal of Natural Products 70

      Pages: 1910-1914

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mangiferin Identified in a Screening Study Guided by Neuraminidase Inhibitory Activity2007

    • Author(s)
      X. Li
    • Journal Title

      Planta Medica 73

      Pages: 1195-1196

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cell Growth and Cell Cycle Inhibitory Activeties of 20-epidiosgenyl Saponin from Calamus insignis2007

    • Author(s)
      T. Ohtsuki
    • Journal Title

      Heterocycles 74

      Pages: 931-936

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Steroidal Saponins from Calamus insignis and Their Cell Growth and Cell Cycle Inhibitory Activities2006

    • Author(s)
      T.Ohtsuki
    • Journal Title

      Bioorganic Medicinal Chemistry 14

      Pages: 659-665

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Kehokorins A-C, Novel Cytotoxic Dibenzofurans Isolated from the Myxomycete Trichia favoginea var. persimilis2006

    • Author(s)
      K.Kaniwa
    • Journal Title

      Tetrahedron Letters 47

      Pages: 1505-1508

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Trichiol and 3-epitrichiol acetate, novel cytotoxic sterols with a 2,6-dioxabicyclo[2.2.2]octan-3-one ring system from the myxomycete Trichia favoginea var. persimilis2006

    • Author(s)
      K.Kaniwa
    • Journal Title

      Tetrahedron Letters 47

      Pages: 4351-4354

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Diterpenes from Leucas aspera Inhibiting Prostaglandin-Induced Contractions2006

    • Author(s)
      S.K.Sadhu
    • Journal Title

      Journal of Natural Products 69

      Pages: 988-994

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Bisindole Alkaloids from Myxomycetes Arcyria denudata and Arcyria obvelata2006

    • Author(s)
      K.Kamata
    • Journal Title

      Journal of Natural Products 69

      Pages: 1252-1254

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Tryptophan 4-aminocinnamamides from a myxomycete Fuligo aurea2006

    • Author(s)
      T.Hosoya
    • Journal Title

      Heterocycles 69

      Pages: 463-468

    • Related Report
      2006 Annual Research Report
  • [Presentation] Search for bioactive natural products targeting signaling molecules in cancer-related biological pathways2007

    • Author(s)
      M. Ishibashi
    • Organizer
      The 6th Princess Chulabhorn International Science Congress
    • Place of Presentation
      バンコク
    • Year and Date
      2007-11-28
    • Related Report
      2007 Annual Research Report
  • [Book] パートナー天然物化学 第2章2007

    • Author(s)
      石橋正己
    • Total Pages
      34
    • Publisher
      南江堂
    • Related Report
      2007 Annual Research Report
  • [Book] Biomaterials from Aquatic and Terrestrial Organisms2006

    • Author(s)
      M.Ishibashi
    • Total Pages
      23
    • Publisher
      Science Publishers, Inc.
    • Related Report
      2006 Annual Research Report
  • [Remarks]

    • URL

      http://www.p.chiba-u.ac.jp/lab/kouzou/

    • Related Report
      2007 Annual Research Report
  • [Patent(Industrial Property Rights)] エレウテリノシドA, B, C2008

    • Inventor(s)
      石橋正己・李暁帆・大槻崇・小谷野喬
    • Industrial Property Rights Holder
      千葉大学
    • Industrial Property Number
      2008-020163
    • Filing Date
      2008-01-31
    • Related Report
      2007 Annual Research Report
  • [Patent(Industrial Property Rights)] トリキオール及び3-エピトリキオールアセタート2007

    • Inventor(s)
      石橋正己・蟹和宏顕
    • Industrial Property Rights Holder
      千葉大学
    • Patent Publication Number
      2007-223940
    • Acquisition Date
      2007-09-06
    • Related Report
      2007 Annual Research Report
  • [Patent(Industrial Property Rights)] ケホコリンD及びケホコリンC2006

    • Inventor(s)
      石橋正己, 渡邊好介, 大槻崇
    • Industrial Property Rights Holder
      千葉大学
    • Industrial Property Number
      2006-321528
    • Filing Date
      2006-11-29
    • Related Report
      2006 Annual Research Report

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Published: 2006-04-01   Modified: 2018-03-28  

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