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昆虫の社会行動を統御するための脳分化の解析

Research Project

Project/Area Number 18047002
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Review Section Science and Engineering
Research InstitutionHokkaido University

Principal Investigator

三浦 徹  Hokkaido University, 大学院・地球環境科学研究院, 准教授 (00332594)

Co-Investigator(Kenkyū-buntansha) 竹内 秀明  東京大学, 大学院・理学系研究科, 助教 (00376534)
尾崎 まみこ  神戸大学, 理学部, 教授 (00314302)
Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥9,700,000 (Direct Cost: ¥9,700,000)
Fiscal Year 2007: ¥4,300,000 (Direct Cost: ¥4,300,000)
Fiscal Year 2006: ¥5,400,000 (Direct Cost: ¥5,400,000)
Keywords社会行動 / カースト / 脳分化 / 分業 / 攻撃性
Research Abstract

本年度は,昨年度同様,社会性昆虫であるオオシロアリを材料にして,ワーカーとソルジャーの分化に伴う脳機能の分化の解析を行った.行動観察により,カーストによる行動走性の相違を検証し,分子基盤や神経生理の解析を後に行う際の機能マッセイ系が構築された.また,この2つのカースト間において,脳の解剖学的相違,組織切片作成による詳細な細胞学的解析により食道下神経節に存在する大顎筋運動ニューロンが兵隊分化に伴い巨大化することを明らかにした.さらに,行動多型を維持する分子基盤の解明を具体的に推し進めるため,2者間の脳における遺伝子発現の相違をディファレンシャルディスプレイ法などにより検出した.その結果発現量に差のある有力な候補遺伝子がいくつか同定された.現在,遺伝子候補に関して,リアルタイムqPCRなどにより発現動態や発現部位の特定を試みるに至った.また,分担者の尾崎は,クロオオアリを用いて,巣仲間認識に関わる体表炭化水素組成の分析およびこの組成を認識する感覚毛における生理学的解析を進め,脳内の触角葉の糸球体において,感覚毛からの情報が集積されることが明らかとなった.また,分担者の竹内はミツバチのキノコ体特異的に発現する遺伝子の解析を精力的に進めた.その結果キノコ体ではエクダイソンシグナルに関わる遺伝子の発現が分業などにより特異的に変化することが突き止やられた.また行動アッセイ法を確立した.これらの知見を集積し,昆虫の高度な社会性を構築する脳内の分子基盤について詳しい考察がされ,成果を国際誌に投稿した.

Report

(2 results)
  • 2007 Annual Research Report
  • 2006 Annual Research Report
  • Research Products

    (36 results)

All 2008 2007 2006 2005

All Journal Article (33 results) (of which Peer Reviewed: 9 results) Presentation (3 results)

  • [Journal Article] Morphological and histological examination of polyphenic wing formation in the pea aphid Acyrthosiphon pisum(Hemiptera, Hexapoda).2008

    • Author(s)
      Ishikawa A, Hongo S, Miura T
    • Journal Title

      Zoomorphology 127

      Pages: 121-133

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Difference in cellulose digestive systems among castes in two termite lineages.2008

    • Author(s)
      Fujita A, Miura T, Matsumoto T
    • Journal Title

      Physiological Entomology 33

      Pages: 73-82

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Sexual dimorphism in the antennal lobe structure of the ant, Camponotus japonicus.2008

    • Author(s)
      M Nishikawa, H Nishino, Y Misaka, M Kubita, E Tsuji, Y Satoji, M Ozaki, F Yokohari
    • Journal Title

      Zool Sci (In press)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Differences in mechanosensory hairs among castes of the damp-wood termite Hodotermopsis sjostedti(Isoptera:Termitidae).2007

    • Author(s)
      Ishikawa Y, Koshikawa S, Miura T
    • Journal Title

      Sociobiology 50

      Pages: 895-907

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Morphological differences between wing morphs of two Macrosiphini aphid species, Acyrthosiphon pisum and Megoura crassicauda(Hemiptera, Aphididae)2007

    • Author(s)
      Ishikawa A, Miura T
    • Journal Title

      Sociobiology 50

      Pages: 881-893

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Save Isoptera. A comment on Inward et al.2007

    • Author(s)
      Lo N, Engel MS, Cameron S, Nalepa CA, Tokuda G, Grimaldi D, Kitade O, Krishna K, Klass K-D, Maekawa K, Miura T, Thompson GJ
    • Journal Title

      Biology Letters 3

      Pages: 562-563

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Histological analysis on fat body development and molting events during soldier differentiation in the damp-wood termite Hodotermopsis sjostedti(Isoptera, Termopsidae)2007

    • Author(s)
      Cornette R, Matsumoto T, Miura T
    • Journal Title

      Zoological Science 24

      Pages: 1066-1074

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Associative learning and discrimination of motion cues in the harnessed honeybee Apis mellifera L2007

    • Author(s)
      Hori S, Takeuchi H, Kubo T
    • Journal Title

      J. Comp. Physiol. A 193

      Pages: 825-833

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] An inhibitory sex pheromone tastes bitter for Drosophola males.2007

    • Author(s)
      F Lacaille, M Hiroi, R Twele, T Inoshita, D Umemoto, G Maniere, F Marion-poll, M Ozaki, W Franke, M Cobb, C Zeve raerts, T Tanimura, J-F Ferveur
    • Journal Title

      PLoS ONE 2

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cloning and expression of a geranylgeranyl diphosphate synthase gene - Insights into the synthesis of termite defense secretion.2007

    • Author(s)
      Hojo M, Matsumoto T, Miura T
    • Journal Title

      Insect Molecular Biology 16

      Pages: 121-131

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Alate differentiation and compound-eye development in the dry-wood termite Neotermes koshunensis (Isoptera, Kalotermitidae).2007

    • Author(s)
      Katoh H, Matsumoto T, Miura T
    • Journal Title

      Insectes Sociaux 54(In press)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] シロアリのカースト分化と遺伝子発現の解析~ソシオゲノミクスへの展望~2007

    • Author(s)
      越川滋行, 三浦徹
    • Journal Title

      日本生態学会誌 57(In press)

    • NAID

      110006277367

    • Related Report
      2006 Annual Research Report
  • [Journal Article] EcR-A expression in the brain and ovary of the honeybee2007

    • Author(s)
      Takeuchi, H., Paul, R.K., Matsuzaka, E., Kubo, T.
    • Journal Title

      Zool. Sci. (in press)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Identification of proteins whose expression is up- or down-regulated in the mushroom bodies in the honeybee brain using proteomics.2007

    • Author(s)
      Uno, Y., Fujiyuki, T., Takeuchi, H., Morioka, M., Kubo, T.
    • Journal Title

      FEBS Lett. 581(1)

      Pages: 97-101

    • Related Report
      2006 Annual Research Report
  • [Journal Article] アリのケミカルコミュニケーション2007

    • Author(s)
      和田綾子, 尾崎まみこ
    • Journal Title

      比較生理生化学 24(1)

      Pages: 3-17

    • Related Report
      2006 Annual Research Report
  • [Journal Article] A caste-specific cytochrome P450in the damp-wood termite Hodotermopsis sjostedti (Isoptera, Termopsidae).2006

    • Author(s)
      Cornette R, Koshikawa, S, Hojo M, Matsumoto T, Miura T
    • Journal Title

      Insect Molecular Biology 15

      Pages: 235-244

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Analysis of the genetic diversity of Nasutitermes coxipoensis (Isoptera : Termitidae) in natural fragments of Brazilian cerrado savanna using AFLP markers.2006

    • Author(s)
      Garcia J, Maekawa K, Constantino R, Matsumoto T, Miura T
    • Journal Title

      Sociobiology 48

      Pages: 267-279

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Caste development and division of labor in the processional nasute termite Hospitalitermes medioflavus in Borneo.2006

    • Author(s)
      Miura T
    • Journal Title

      TROPICS 15

      Pages: 275-278

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Morphological differences between sexes in the ponerine ant Diacamma sp. (Diacamma : Ponerinae).2006

    • Author(s)
      Okada Y, Tsuji K, Miura T
    • Journal Title

      Sociobiolgy 48

      Pages: 527-541

    • Related Report
      2006 Annual Research Report
  • [Journal Article] シロアリの消化共生系と栄養生態2006

    • Author(s)
      藤田愛, 三浦徹
    • Journal Title

      しろあり 143

      Pages: 8-13

    • NAID

      40007145946

    • Related Report
      2006 Annual Research Report
  • [Journal Article] シロアリ兵隊における防衛物質の合成2006

    • Author(s)
      北條優, 三浦徹
    • Journal Title

      しろあり 145

      Pages: 3-8

    • NAID

      40007410249

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Gene expression in the Honeybee Brain Mushroom Body and its Gene Orthologues.2006

    • Author(s)
      Hideaki Takeuchi
    • Journal Title

      Evolution of Nervous Systems (Elsevier) 1

      Pages: 457-469

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Prevalence and phylogeny of Kakugo virus, a novel insect picorna-like virus that infects the honeybee (Apis mellifera L.), under various colony conditions.2006

    • Author(s)
      Fujiyuki, T., Ohka, S., Takeuchi, H., Ono, M., Nomoto, A., Kubo, T.
    • Journal Title

      J. Virol. 80(23)

      Pages: 11528-11538

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Expression of two ecdysteroid-regulated genes, Broad-Complex and E75, in the brain and ovary of the honeybee (Apis mellifera L.).2006

    • Author(s)
      Paul, R.K., Takeuchi, H., Kubo, T.
    • Journal Title

      Zool. Sci. 23(12)

      Pages: 1085-1092

    • NAID

      130000017061

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Carbohydrate metabolism genes and pathways in insects : insights from the honey bee genome.2006

    • Author(s)
      Kunieda, T., Fujiyuki, T., Kucharski, R., Foret, S., Ament, S.A., Toth, A.L., Ohashi, K, Takeuchi, H, Kamikouchi, A., Kage, E., Morioka, M., Beye, M., Kubo, T., Robinson, G.E., Maleszka, R.
    • Journal Title

      Insect Mol. Biol 15(5)

      Pages: 563-576

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Differential expression of HR38 in the mushroom bodies of the honeybee brain depends on the caste and division of labor.2006

    • Author(s)
      Yamazaki, Y., Shirai, K., Paul, R.K., Fujiyuki, T., Wakamoto, A., Takeuchi, H., Kubo, T.
    • Journal Title

      FEBS Lett. 580(11)

      Pages: 2667-2770

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Associative visual learning, color discrimination, and chromatic adaptation in the harnessed honeybee Apis mellifera L.2006

    • Author(s)
      Hori, S., Takeuchi, H., Arikawa, K., Kinoshita, M., Ichikawa, N., Sasaki, M., Kubo, T.
    • Journal Title

      J. Comp. Physiol. A 192(7)

      Pages: 691-700

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Division of labor in the honey bee (Apis mellifera) : The role of tyramine beta-hydroxylase2006

    • Author(s)
      Lehman, H.K., Schulz, D.J., Barron, A.B., S.A., Wraight, L., Hardison, C., Whitney, S., Takeuchi, H., Paul, R.K., Robinson, G.E.
    • Journal Title

      J. Exp. Biol. 209(14)

      Pages: 2774-2784

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Insights into social insects from the genome of the honey bee Apis mellifera.2006

    • Author(s)
      The Honeybee Genome Sequencing Consortium: アノテーション作業に参加
    • Journal Title

      Nature 443(7114)

      Pages: 931-949

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Behabioral and electrophysiopogical investigation on taste response of the termite Zootermopsis nevadensis to wood extractives2006

    • Author(s)
      Wakako Omura, Mamiko Ozaki, Ryohei Yamaoka
    • Journal Title

      J. Wood Sci. 52

      Pages: 261-264

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Scale free dynamics involved in the locomotion activity of ant and mouse2006

    • Author(s)
      Kazumitsu Hanai, Mamiko Ozaki, Daigo Yamauchi, Yasuhiro Nakatomi, Chihiro Yokoyama, Keniji Fukui
    • Journal Title

      WSEAS Transactions on Biology and Biomedicine 3

      Pages: 511-515

    • Related Report
      2006 Annual Research Report
  • [Journal Article] 何が敵・味方を教えるか アリ社会を支える固体識別化学センサー2006

    • Author(s)
      尾崎まみこ, 城所碧
    • Journal Title

      化学と生物 44(9)

      Pages: 633-636

    • Related Report
      2006 Annual Research Report
  • [Journal Article] アリのコロニー識別行動のビデオ解析2005

    • Author(s)
      花井一光, 山内大吾, 里路裕司, 中川恵津子, 大橋乙予, 尾崎まみこ, 山岡亮平
    • Journal Title

      Studia humana et naturalia 39

      Pages: 90-98

    • NAID

      110004910744

    • Related Report
      2006 Annual Research Report
  • [Presentation] Molecular basis for social behavior and caste differentiation in termites2007

    • Author(s)
      Miura T
    • Organizer
      Neuro2007
    • Place of Presentation
      横浜市,横浜パシフィコ
    • Year and Date
      2007-09-11
    • Related Report
      2007 Annual Research Report
  • [Presentation] Juvenile hormone action during soldier differentiation in the damp-wood termtie2007

    • Author(s)
      Miura T, Cornette R
    • Organizer
      JH9
    • Place of Presentation
      ヨークシャー博物館,York,UK
    • Year and Date
      2007-08-09
    • Related Report
      2007 Annual Research Report
  • [Presentation] Analysis of adaptive behaviors of small fishes by using combination of mathematical modeling and molecular biological approach2007

    • Author(s)
      Suehiro Y, Imada H, Okuyama T, Kubo T, Takeuchi H
    • Organizer
      Second International Symposium on Mobiligence
    • Place of Presentation
      淡路島,淡路夢舞台国際会議場
    • Related Report
      2007 Annual Research Report

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Published: 2006-04-01   Modified: 2018-03-28  

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