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生殖細胞のインプリンティングおよび減数分裂におけるHP1γの機能解明

Research Project

Project/Area Number 18051008
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionKanazawa University

Principal Investigator

浅野 雅秀  Kanazawa University, 学際科学実験センター, 教授 (50251450)

Co-Investigator(Kenkyū-buntansha) 成瀬 智恵  金沢大学, 学際科学実験センター, 助教 (30372486)
Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥4,400,000 (Direct Cost: ¥4,400,000)
Fiscal Year 2007: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 2006: ¥2,400,000 (Direct Cost: ¥2,400,000)
Keywords生殖細胞 / エピジェネティックス / インプリンティング / 遺伝子改変マウス / 不妊
Research Abstract

1)HP1γ変異マウスの減数分裂期の生殖細胞の解析
HP1γ変異マウスは雌雄ともに不妊であったので,生殖細胞の形成過程を調べた結果,HP1γ変異マウスの生殖細胞ではレプトテン期からザイゴテン期への移行に遅延が見られ,ディプロテン期に進む生殖細胞数が著しく減少していることがわかった。また,減数分裂期にはHP1γは主にセントロメアとXY-bodyに局在しており,減数分裂期の染色体におけるヒストン修飾の状態を解析したところ,セントロメアにおけるピストンのメチル化が低下していることがわかった。このように、HP1γはエピジェネティック制御を介して減数分裂に重要な役割を果たしている可能性が示唆された。
2)HP1γ変異マウスの始原生殖細胞(PGC)の解析
HP1γ変異マウスの生殖細胞は減数分裂期だけでなく,発生過程を遡って解析するとE7.5-8.0のエピブラストにおいてすでにPGCの数が減少していることがわかった。まず,この時期のPGCの中胚葉から内胚葉への移動は正常に起こっていた。そこでE7.5-8.0のPGCの単一細胞RT-PCR法により,Blimp1陽性/Stella陽性のPGCにおける遺伝子発現を調べた。この時期のPGCではHP1γが強く発現しており,何らかの機能を果たしていることが示唆されたが,体細胞マーカーであるHoxb1の発現はHP1γ欠損PGCでも抑制されていたので,少なくともPGCへの分化は正常に起こっていることがわかった。

Report

(2 results)
  • 2007 Annual Research Report
  • 2006 Annual Research Report
  • Research Products

    (22 results)

All 2008 2007 2006 2003 Other

All Journal Article (15 results) (of which Peer Reviewed: 8 results) Presentation (4 results) Book (1 results) Remarks (1 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] A novel β-myosin heavy chain gene mutation, p. Met531Arg, identified in isolated left ventricular noncompaction in humans, resulted in left ventricular hypertrophy that progressed to dilation in a mouse model.2008

    • Author(s)
      Kaneda, T., et. al.
    • Journal Title

      Clinic. Sci. 114

      Pages: 431-440

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Suppressive Role of Leukocyte Cell-Derived Chemotaxin 2 in Mouse Anti-Type II Collagen Antibody-Induced Arthritis.2008

    • Author(s)
      Okumura, A., et. al.
    • Journal Title

      Arthritis & Rheumatism 58

      Pages: 413-421

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Wtap is required for differentiation of endoderm and mesoderm in the mouse embryo.2008

    • Author(s)
      Fukusumi, Y., et. al.
    • Journal Title

      Dev. Dyn. 237

      Pages: 618-629

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] EphA2 is an essential mediator of ultraviolet radiation-induced apoptosis.2008

    • Author(s)
      Zhang, G., et. al.
    • Journal Title

      Cancer Research 68

      Pages: 1691-1696

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Neuroprotective actions of PIKE-L by inhibition of SET proteolytic degradation by asparagine endopeptidase (AEP).2008

    • Author(s)
      Liu, Z.
    • Journal Title

      Mol. Cell (In press)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Legumain/asparaginyl endopeptidase controls extracellular matrix remodeling through the degradation of fibronectin in mouse renal proximal tubular cells.2007

    • Author(s)
      Morita, Y., et. al.
    • Journal Title

      FEBS Lett 581

      Pages: 1417-1424

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of IgA nephropathy-like disease with high serum IgA levels and increased proportion of polymeric IgA in β-1,4-galactosyltran sferase-deficinet mice.2007

    • Author(s)
      Nishie, T., et. al.
    • Journal Title

      IgA Nephropathy Today: Contrib. Nephrol. 157

      Pages: 125-128

    • Related Report
      2007 Annual Research Report
  • [Journal Article] A novel gene trapping for identifying genes expressed under the control of specific transcription factors.2007

    • Author(s)
      Naruse, C., et. al.
    • Journal Title

      Biochem. Biophys. Res. Commun. 361

      Pages: 109-115

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Neural-specific ablation of the scaffold protein JSAP1 in mice causes neonatal death.2007

    • Author(s)
      Iwanaga, A., et. al.
    • Journal Title

      Neurosci. Lett. 429

      Pages: 43-48

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of immunoglobulin A nephropathy-like disease in β-1,4-galactosyltransferase-I deficient mice2007

    • Author(s)
      Nishie, T., et al.
    • Journal Title

      Am.J.Pathol. 170

      Pages: 447-459

    • Related Report
      2006 Annual Research Report
  • [Journal Article] 38 is critical for social behavior by regulating oxytocin secretion2007

    • Author(s)
      Jin, D., et al.
    • Journal Title

      Nature 446

      Pages: 41-45

    • Related Report
      2006 Annual Research Report
  • [Journal Article] RAGE control of diabetic nephropathy in a mouse model : Effects of RAGE gene disruption and administration of low-molecular weight heparin2006

    • Author(s)
      Myint, K-M., et al.
    • Journal Title

      Diabetes 55

      Pages: 2510-2522

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Interleukin-1 dependent sequential chemokine expression and inflammatory cell infiltration in ischemia-reperfusion injury2006

    • Author(s)
      Furuichi, K., et al.
    • Journal Title

      Crit.Care.Med. 34

      Pages: 2447-2455

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Expression and distribution of JNK/SAPK-associated scaffold protein JSAP1 in developing and adult mouse brain2006

    • Author(s)
      Miura, E., et al.
    • Journal Title

      J.Neurochem. 97

      Pages: 1431-1446

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Early increase in mRNA levels of pro-inflammatory cytokines and their interactions in the mouse hippocampus after transient global ischemia2006

    • Author(s)
      Zhu, Y., et al.
    • Journal Title

      Neurosci.Lett. 393

      Pages: 122-126

    • Related Report
      2006 Annual Research Report
  • [Presentation] 始原生殖細胞の発生と分化におけるHP1γの機能解析2007

    • Author(s)
      阿部可奈恵, ら
    • Organizer
      日本分子生物学会/日本生化学会
    • Place of Presentation
      横浜
    • Year and Date
      2007-12-12
    • Related Report
      2007 Annual Research Report
  • [Presentation] 生殖細胞におけるHP1γの機能解析2007

    • Author(s)
      成瀬智恵, ら
    • Organizer
      日本エピジェネティクス研究会
    • Place of Presentation
      大阪
    • Year and Date
      2007-06-15
    • Related Report
      2007 Annual Research Report
  • [Presentation] Heterochromatin Protein 1 (HP1) γ;は生殖細胞の形成や減数分裂において重要な役割を持つ2007

    • Author(s)
      成瀬智恵, ら
    • Organizer
      日本発生生物学会/日本細胞生物学会
    • Place of Presentation
      福岡
    • Year and Date
      2007-05-28
    • Related Report
      2007 Annual Research Report
  • [Presentation] 生殖細胞形成におけるHeterochromatin Protein 1 (HP1) γの機能解2007

    • Author(s)
      阿部可奈恵, ら
    • Organizer
      日本実験動物学会
    • Place of Presentation
      東京
    • Year and Date
      2007-05-25
    • Related Report
      2007 Annual Research Report
  • [Book] IgA Nephropathy Today : Contrib. Nephrol.2006

    • Author(s)
      Nishie, T., et al.
    • Publisher
      Karger, Basel(In press)
    • Related Report
      2006 Annual Research Report
  • [Remarks]

    • URL

      http://web.kanazawa-u.ac.jp/%7Easrc/index.html

    • Related Report
      2007 Annual Research Report
  • [Patent(Industrial Property Rights)] IgA腎症の治療薬のスクリーニング方法2003

    • Inventor(s)
      浅野 雅秀,他2名
    • Industrial Property Rights Holder
      金沢大学TLO
    • Filing Date
      2003-04-08
    • Acquisition Date
      2006-06-09
    • Related Report
      2006 Annual Research Report

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Published: 2006-04-01   Modified: 2018-03-28  

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