Multifunctional Nano-Scanning Electrochemical Microscopy

• Project/Area Number: 18101006
• Research Category: Grant-in-Aid for Scientific Research (S)
• Allocation Type: Single-year Grants
• Research Field: Microdevices/Nanodevices
• Research Institution: Tohoku University
• Principal Investigator: MATSUE Tomokazu Tohoku University, 大学院・環境科学研究科, 教授 (70173797)
• Co-Investigator(Kenkyū-buntansha): MIZUTANI Fumio 兵庫県立大学, 大学院・物質理学研究科, 教授 (80118603) ; YANAGISAWA Teruyuki 東北大学, 大学院・医学系研究科, 教授 (90133941) ; SHIKU Hitoshi 東北大学, 大学院・環境科学研究科, 准教授 (10361164) ; YASUKAWA Tomoyuki 兵庫県立大学, 大学院・物質理学研究科, 准教授 (40361167) ; YAMADA Hiroshi 防衛大学校, 応用化学科, 准教授 (10545974)
• Co-Investigator(Renkei-kenkyūsha): AKIBA Uichi 秋田大学, 工学資源学部, 准教授 (60184107)
• Project Period (FY): 2006 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥93,340,000 (Direct Cost: ¥71,800,000、Indirect Cost: ¥21,540,000); Fiscal Year 2009: ¥16,120,000 (Direct Cost: ¥12,400,000、Indirect Cost: ¥3,720,000); Fiscal Year 2008: ¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000); Fiscal Year 2007: ¥25,610,000 (Direct Cost: ¥19,700,000、Indirect Cost: ¥5,910,000); Fiscal Year 2006: ¥33,800,000 (Direct Cost: ¥26,000,000、Indirect Cost: ¥7,800,000)
• Keywords: マイクロ化学システム / 単一細胞計測 / 走査型プローブ顕微鏡 / 修飾電極 / 微小電極 / パッチクランプ / 膜 / イオンチャネル / シグナル伝達 / イオンチャンネル / 膜受容体

Research Abstract

The multifunctional micro/nano scanning electrochemical imaging system (NanoSECM) was developed and utilized for the single-cell diagnostics. We fabricated multifunctional micro/nano-structured probe electrodes and developed a novel feedback control between the electrode and sample surfaces at nanometer levels. By using NanoSECM with these new technologies, we successfully attained the fast and high resolution and simultaneous imaging of live cells to disclose the cell functions.

Research Products

• [Journal Article] Simultaneous Noncontact Topography and Electrochemical Imaging by SEC M/SICM Featuring Ion Current Feedba ck Regulation. (2010)
  • Author(s): Y.Takahashi, A.I.Shev chuk, P.Novak, Y.Murakami, H.Shiku, Y.E.Korchev, T.Matsue
  • Journal Title: J.Am.Chem.Soc. 132 Pages: 10118-10126
  • Peer Reviewed
• [Journal Article] Y. Takahashi, Y. Murakami, K. Nagamine, H. Shiku, S. Aoyagi, T. Yasukawa, M. Kanzaki, T. Matsue (2010)
  • Author(s): Topographic Imaging of Convoluted Live Cells by Scanning Ion Conductance Micr oscopy in a Standing Approach Mode
  • Journal Title: Phys.Chem.Chem.Phys. 12 Pages: 10012-17
  • Peer Reviewed
• [Journal Article] Electrochemical Monitoring of Hydrogen Peroxide Released from Leucocytes on Horseradish Peroxidase Redox Polymer Coated Electrode Chip. (2010)
  • Author(s): K.Y. Inoue, K.Ino, H.Shiku, S.Kasai, T.Yasukawa, F.Mizutani, T.Matsue
  • Journal Title: Biosens.Bioelectron. 25 Pages: 1723-1728
  • Peer Reviewed
• [Journal Article] Chronoamperometric characterization of secreted alkaline phosphatase from single cell entrapped in a poly(dimethylsiloxisane)microwell. (2010)
  • Author(s): H.Shiku, J.Suzuki, T.Murata, K.Ino, T.Matsue
  • Journal Title: Electrochim.Acta 55(印刷中)
  • Peer Reviewed
• [Journal Article] Transfected Single-Cell Imaging by Scan ping Electrochemical Optical Microscopy with Shear Force Feedback Regulation. (2009)
  • Author(s): Y.Takahashi, H.Shiku, T.Murata, T.Yasukawa, T.Matsue
  • Journal Title: Anal.Chem. 81 Pages: 9674-9681
  • Peer Reviewed
• [Journal Article] Electrochemical Single-Cell Gene Expression Assay Combining Dielectrophoretic M anipulation with Secreted Alkaline Phospha tase Reporter System. (2009)
  • Author(s): T.Murata, T.Yasukawa, H.Shiku, T.Matsue
  • Journal Title: Biosens. Bioelectron. 25 Pages: 913-919
  • Peer Reviewed
• [Journal Article] Electrochemical Detection of Epidermal Growth Factor Receptors on a Single Living Cell Surface by Scanning Electrochemical Microscopy. (2009)
  • Author(s): Y.Takahashi, T.Miyamoto, H.Shiku, R.Asano, T.Yasukawa, I.Kumagai, T.Matsue
  • Journal Title: Anal.Chem. 81 Pages: 2785-2790
  • Peer Reviewed
• [Journal Article] Electrochemical gene-function analysis for single cells with addressable microelectrode/microwell arrays. (2009)
  • Author(s): Z.Y.Lin, Y.Takahashi, T.Murata, M.Takeda, K.Ino, H.Shiku, T.Matsue
  • Journal Title: Angew.Chem.Int.Ed.Engl. 48 Pages: 2044-2046
  • Peer Reviewed
• [Journal Article] Mitsugumin 53-mediated maintenance of K^+ currents in cardiac myocytes. (2009)
  • Author(s): Masumiya H., Asaumi Y., Nishi M., Minamisawa S., Adachi-Akahane S., Yoshida M., Kangawa K., Ito K., Kagaya Y., Yanagisawa T., Yamazaki T., Ma J., Takeshima H.
  • Journal Title: Channels 3 Pages: 6-11
  • Peer Reviewed
• [Journal Article] Manipulation of Microparticles for Construction of Array Patterns by Negative Dielectrophoresis Using Mutilayered Array and Grid Electrodes. (2009)
  • Author(s): K.Ino, H.Shiku., F. Ozawa, T.Yasukawa, T.Matsue
  • Journal Title: Biotech.Bioeng. 104 Pages: 709-718
  • Peer Reviewed
• [Journal Article] Electrochemical Single-Cell Gene Expression Assay Combining Dielectrophoretic Manipulation with Secreted Alkaline Phosphatase Reporter System (2009)
  • Author(s): T.Murata, T.Yasukawa, H.Shiku, T.Matsue
  • Journal Title: Biosens.Bioelectron. 25 Pages: 913-919
  • Peer Reviewed
• [Journal Article] Transfected Single-Cell Imaging by Scanning Electrochemical Optical Microscopy with Shear Force Feedback Regulation. (2009)
  • Author(s): Y.Takahashi, H.Shiku, T.Murata, T.Yasukawa, T.Matsue
  • Journal Title: Anal.Chem. 81 Pages: 9674-9681
  • Peer Reviewed
• [Journal Article] Enzyme Immobilization on Poly(dimethysiloxane)Layer for Amperometric Sensing of Glucose (2009)
  • Author(s): Tomoyuki Yasukawa, Eiji Maekawa, Fumio Mizutani
  • Journal Title: Anal.Sci. 25 Pages: 1159-1162
  • Peer Reviewed
• [Journal Article] Detection of hormone active chemicals using genetically engineered yeast cells and microfluidic devices with interdigitated array electrodes. (2009)
  • Author(s): K.Ino, Y.Kitagawa, T.Watanabe, H.Shiku, M.Koide, T.Itayama, T.Matsue
  • Journal Title: Electrophoresis 30 Pages: 3406-3412
  • Peer Reviewed
• [Journal Article] (2009)
  • Author(s): 珠玖仁、伊野浩介、末永智一
  • Journal Title: シングルセル解析の最前線 第4章「mRNAをターゲットとしたデジタル精密計測技術の開発」(シーエムシー出版) Pages: 223-228
• [Journal Article] Electrochemical Characterization of Enzymatic Activity of Yeas t Cells Entrapped in a Poly(dimethylsiloxane) Microwell on the Basis of Limited Diffusion System. (2009)
  • Author(s): H. Shiku, S. Goto, S. B Jung, K. Nagamine, M. Koide, T. Itayama, T. Yasukawa, T. Mat sue
  • Journal Title: Analyst 134 Pages: 182-187
  • Peer Reviewed
• [Journal Article] A microfluidic dual capillary probe to collect mRNA from adherent cells and spheroids (2009)
  • Author(s): H. Shiku, T. Yamakawa, Y. Na shimoto, Y. Takahashi, Y. To risawa, T. Yasukawa, T. Ito-Sasaki, M. Yokoo, H. Abe, H. Kambara, T. Matsue
  • Journal Title: Anal. Biochem 385 Pages: 138-142
  • Peer Reviewed
• [Journal Article] Electrochemical Detection of Epidermal Growth Factor Receptors on a Single Living Cell Surface by Scanning Electrochemical M icroscopy (2009)
  • Author(s): Y. Takahashi, T. Miyamoto, H. Shiku, R. Asano, T. Yasuka wa, I. Kumagai, T. Matsue
  • Journal Title: Anal. Chem. 81 Pages: 2785-2790
  • Peer Reviewed
• [Journal Article] Development of Electrochemical Reporter Assay Based on HeLa Cells Transfected with Vector Plasmids Coding Various Responsive Elements. (2009)
  • Author(s): H. Shiku, M. i Takeda, T. Mur ata, U. Akib, F. Hamada, T. Matsue
  • Journal Title: Anal. Chim. Acta 639(印刷中)
  • Peer Reviewed
• [Journal Article] Takemoto J, Masumiya H, Nunoki K, Sato T, Nakagawa H, Ikeda Y, Arai, Yanagisawa Y (2008)
  • Author(s): Potentiation of potassium currents by beta-adrenoceptor agonists in human urinary bladder smooth muscle cells : a possible electrical mechanism of relaxation.
  • Journal Title: Pharmacology 81 Pages: 251-258
  • Peer Reviewed
• [Journal Article] Fabrication of a shear force-based ion-se lective capillary probe for scanning electro chemical microscopy. (2008)
  • Author(s): H.Yamada, Y.Ikut a Y, T.Koike T, T.Matsue
  • Journal Title: Chem.Lett. 37 Pages: 392-393
  • Peer Reviewed
• [Journal Article] Construction of Time-Lapse Scanning Electrochemical Microscopy with Temperature Control and Its Application To Evaluate the Preservation Effects of Antifreeze Proteins on Living Cells (2008)
  • Author(s): Y. Hirano, Y. Nishimiya, K. Kowata, F. Mizutani, S. Tsuda, Y. Komatsu
  • Journal Title: Anal. Chem. 80 Pages: 9349-9354
  • Peer Reviewed
• [Journal Article] Fabrication of a shear force-based ion-selective capillary probe for scanning electrochemical microscopy (2008)
  • Author(s): H. Yamada, Y. Ikuta Y, T. Koike T, T. Matsue
  • Journal Title: Chem. Lett. 37 Pages: 392-393
  • NAID: 10021079193
  • Peer Reviewed
• [Journal Article] Cell-Based Electrochemical Assay for Endotoxin Using a Secreted Alkaline Phosphatase Reporter System. (2008)
  • Author(s): K. Y. Inoue, T. Yasukawa, H. Shiku, T. Matsue
  • Journal Title: Electrochemistry 8 Pages: 525-528
  • NAID: 10021027666
  • Peer Reviewed
• [Journal Article] Electrophoretic cell manipulation and electrochemical gene-function analysis based on a yeast two-hybrid system in a microfluidic device (2008)
  • Author(s): T. Yasukawa, K. Nagamine, Y. Horiguchi, H. Shiku, M. Koide, T. Itayama, F. Shiraishi, T. Matsue
  • Journal Title: Anal. Chem. 80 Pages: 3722-3727
  • Peer Reviewed
• [Journal Article] Fabrication of a shear force-based ion-selective capillary probe for s canning electrochemical microscopy (2008)
  • Author(s): H. Yamada, Y. Ikuta, T. Koike, T. Matsue
  • Journal Title: Chem. Lett. 37 Pages: 392-393
  • NAID: 10021079193
  • Peer Reviewed
• [Journal Article] Patterning of gold surfaces with hexadecanethiol by shear force-based scanning capillary microscopy (2008)
  • Author(s): H. Yamada, K. Isaka, T. Koike, T. Matsue
  • Journal Title: Chem. Lett 37 Pages: 412-413
  • NAID: 10021079404
  • Peer Reviewed
• [Journal Article] Construction of time-lapse scanning electrochemical microscopy with to mperature control and its application to evaluate the preservation effect of antifreeze proteins on living cells (2008)
  • Author(s): Y. Hirano, Y. Nishimiya, K. Kowata, F. Mizutani, S. Tsuda, Y. Komatsu
  • Journal Title: Anal. Chem. 80 Pages: 9349-9354
  • Peer Reviewed
• [Journal Article] Potentiation of potassium currents by beta-adrenoceptor agonists in human urinary bladder smooth muscle cells: a possible electrical mechanism of relaxation (2008)
  • Author(s): J Takemoto, H Masumiya, K Nunoki, T Sato, T Nakagawa, Y Ikeda, Y Arai, Yanagisawa
  • Journal Title: Pharmacology 81 Pages: 251-258
  • Peer Reviewed
• [Journal Article] 質疑応答電位依存性Ca2+チャネル分類とCa拮抗薬の差異化 (2008)
  • Author(s): 柳澤輝行
  • Journal Title: 日本医事新報 4368 Pages: 96-98
• [Journal Article] (2008)
  • Author(s): H. Shiku, K. Nagamine, T. Kaya, T. Yasukawa, T. Matsue
  • Journal Title: Handbook of Bioelectrochemistry, Chapter 7 "Whole-Cell Biosensors"(John Wiley) Pages: 249-266
• [Journal Article] Measurement of Gene Expression from Single Adherent Cells and Spheroids Colle cted Using Fast Electrical Lysis. (2007)
  • Author(s): Y Nashimoto, Y.Takahashi, T.Yamakawa, Y.Torisawa, T.Yasukawa, T.Ito-Sasaki, Mi Yokoo, H.Abe, H.Shiku, H.Kambara, T.Matsue
  • Journal Title: Anal.Chem. 79 Pages: 6823-6830
  • Peer Reviewed
• [Journal Article] Microcontact printed diaphorase monolay er on glass characterized by atomic force microscopy and scanning electrochemical microscopy (2007)
  • Author(s): H.Q.Luo, H.Shiku, A.Kumagai, Y.Takahashi, T.Yasukawa, T.Matsue
  • Journal Title: Electrochemistry Communications 9 Pages: 2703-2708
  • Peer Reviewed
• [Journal Article] Electrochemical screening of recombinant protein solubility in Escherichia coli using scanning electrochemical microscopy (SECM) (2007)
  • Author(s): K.Nagamine, S.Onodera, A.Kurihara, T.Yasukawa, H.Shiku, R.Asano, I.Kumagai, T.Matsue
  • Journal Title: Biotechnology and bioengineering 96 Pages: 1008-1013
  • Peer Reviewed
• [Journal Article] Microcontact printed diaphorase monolayer on glass characterized by atomic force microscopy and scanning electrochemical microscopy (2007)
  • Author(s): H. Q. Luo, H. Shiku, A. Kumagai, Y. Takahashi, T. Yasukawa, T. Matsue
  • Journal Title: Electrochem. Commun. 9 Pages: 2703-2708
  • Peer Reviewed
• [Journal Article] Electrochemical screening of recombinant protein solubility in Escherichia coli using scanning electrochemical microscopy (SECM) Biotech (2007)
  • Author(s): K. Nagamine, S. Onodera, A. Kurihara, T. Yasukawa, H. Shiku, R. Asano, I. Kumagai, T. Matsue
  • Journal Title: Bioeng 96 Pages: 1008-1013
  • Peer Reviewed
• [Journal Article] Measurement of Gene Expression from Single Adherent Cells and Spheroids Collected Using Fast Electrical Lysis (2007)
  • Author(s): Y Nashimoto, Y. Takahashi, T. Yamakawa, Y. Torisawa, T. Yasukawa, T. Ito-Sasaki, M. i Yokoo, H. Abe, H. Shiku, H. Kambara, T. Matsue
  • Journal Title: Anal. Chem. 79 Pages: 6823-6830
  • Peer Reviewed
• [Journal Article] A Competitive Immunochromatographic Assay for Testosterone Based on Electrochemical Detection (2007)
  • Author(s): Kumi Inoue, Pascal Ferrante, Yu Hirano, Tomoyuki Yasukawa, Hitoshi Shiku, Tomokazu Matsue
  • Journal Title: Talanta 73 Pages: 886-892
  • Peer Reviewed
• [Journal Article] Regulation and characterization of the polarity of cells embedded in a reconstructed basement matrix using a three-dimensional micro-culture system (2007)
  • Author(s): Torisawa, Y. -S., Nashimoto, Y., Yasukawa, T., Shiku, H., Matsue, T.
  • Journal Title: Biotechnology and Bioengineering 97 Pages: 615-621
  • Peer Reviewed
• [Journal Article] Microcontact printed diaphorase monolayer on glass characterized by atomic force microscopy and scanning electrochemical microscopy (2007)
  • Author(s): Hong Qun Luo, Hitoshi Shiku, Ayako Kumagai, Yasufumi Takahashi, Tomoyuki Yasukawa, Tomokazu Matsue
  • Journal Title: Electrochemistry Communications 9 Pages: 2703-2708
  • Peer Reviewed
• [Journal Article] 第3編微小(マイクロ)アナリシスシステム, 第2章(μTAS)「マイクロフルーイディックシステムを用いた細胞デバイス」 (2007)
  • Author(s): 鳥澤勇介, 安川智之, 珠玖仁, 末永智一
  • Journal Title: バイオセンサ・ケミカルセンサ事典(テクノシステム) Pages: 787-797
• [Journal Article] Multicellular spheroid array chip to realize spheroid formation, culture and viability assay (2007)
  • Author(s): Y.Torisawa, et al.
  • Journal Title: Biomaterials 28・3 Pages: 559-566
• [Journal Article] Electrochemical screening of recombinant protein solubility in Escherichia coli using scanning electrochemical microscopy (SECM) (2007)
  • Author(s): K.Nagamine, et al.
  • Journal Title: Biotechnol. Bioeng. 96・5 Pages: 1008-1013
• [Journal Article] Patterning and Encapsulation with Colloidal Particles into Hydrogel using Negative-Dielectrophoretic Method" (2007)
  • Author(s): M.Suzuki, et al.
  • Journal Title: Langmuir, (manuscript ID la063075a) (in print)(in press)
• [Journal Article] Enzyme Immunosensing of Pepsinogens 1 and 2 by Scanning Electrochemical Microscopy (2007)
  • Author(s): T.Yasukawa, et al.
  • Journal Title: Biosensors and Bioelectronics (in print)(in press)
• [Journal Article] Hyodrogen Bonding Interaction between Aminopurinethiol-Monolayers and Oligonucleotide by QCM and XPS Measurements (2007)
  • Author(s): Y.Sato, et al.
  • Journal Title: Sensors Actuators B 121・1 Pages: 214-218
• [Journal Article] Electrochemically Amplified Detection for Lipopolysaccharide Using Ferroceneboronic (2007)
  • Author(s): D.Kato, et al.
  • Journal Title: Acid, Biosenors Bioelectron 22・7 Pages: 1527-1531
• [Journal Article] Somatodendritic localization of EFA6A, a guanine nucleotide exchange factor for ADP-ribosylation factor 6, and its possible interaction with α-actinin in dendritic spines (2007)
  • Author(s): H.Sakagami, et al.
  • Journal Title: European Journal of Neuroscience (doi : 10.1111) 25・3 Pages: 618-628
• [Journal Article] Topographic, Electrochemical, and O ptical Images Captured Using Standing Approach Mode Scanning Electrochemic al/Optical Microscopy. (2006)
  • Author(s): Y.Takahashi, Y.Hirano, T.Yasukawa, H.Shiku, H.Yamada, T.Matsue
  • Journal Title: Langmuir 22 Pages: 10299-10306
  • Peer Reviewed
• [Journal Article] Topographic, Electrochemical, and Optical Images Captured Using Standing Approach Mode Scanning Electrochemical/Optical Microscopy (2006)
  • Author(s): Y. Takahashi, Y. Hirano, T. Yasukawa, H. Shiku, H. Yamada, T. Matsue
  • Journal Title: Langmuir 22 Pages: 10299-10306
  • Peer Reviewed
• [Journal Article] Topographic, Electrochemical, and Optical Images Captured using Standing Approach Mode Scanning Electrochemical/Optical Microscopy" (2006)
  • Author(s): Y.Takahashi, et al.
  • Journal Title: Langmuir 22・25 Pages: 10299-10306
• [Journal Article] Oxygen consumption of cell suspension in a poly(dimethylsiloxiane) (PDMS) microchannel estimated by scanning electrochemical microscopy (2006)
  • Author(s): T.Saito, et al.
  • Journal Title: Analyst 131・9 Pages: 1006-1011
• [Journal Article] 呼吸量測定によるウシ体外培養胚の品質評価と半自動計測装置の開発 (2006)
  • Author(s): 青柳重夫, ら
  • Journal Title: 分析化学 55・11 Pages: 847-854
• [Journal Article] 走査型電気化学顕微鏡を用いるC反応性タンパクの酵素免疫センシング (2006)
  • Author(s): 安川智之, ら
  • Journal Title: 分析化学 55・12 Pages: 979-985
• [Journal Article] プローブ法(1)走査型電気化学顕微鏡(SECM)・走査型振動電極法(SVET) (2006)
  • Author(s): 青柳重夫, ら
  • Journal Title: Electrochemistry 74・9 Pages: 787-790
• [Journal Article] タンパク質のパターニングと界面現象の解析 (2006)
  • Author(s): 高橋康史, et al.
  • Journal Title: 表面科学 27・10 Pages: 613-616
• [Journal Article] Electrochemical monitoring of cellular signal transduction with secreted alkaline phosphatase reporter system (2006)
  • Author(s): Y.Torisawa, et al.
  • Journal Title: Anal. Chem. 78 Pages: 7625-7631
• [Journal Article] On-Chip Enzyme Immunoassay of a Cardiac Marker Using a Microfluidic Device Combined with a Portable Surface Plasmon Resonance System (2006)
  • Author(s): R.Kurita, et al.
  • Journal Title: Anal. Chem. 78・15 Pages: 5525-5531
• [Journal Article] Oxytocin-induced phasic and tonic contractions are modulated by the contractile machinery rather than the quantity of oxytocin receptor. (2006)
  • Author(s): M.Kawamata, et al.
  • Journal Title: Am J Physiol Endocrinol Metab, (doi : 10.1152)
• [Journal Article] R ecent advances in molecular pharmacology of the histamine systems : Roles of C-terminal Tails of Histamine Receptors (2006)
  • Author(s): K.Atsuo, et al.
  • Journal Title: Journal of Pharmacological Sciences 101・1 Pages: 7-11
• [Journal Article] Electrochemical Detection of Epidermal Growth Factor Receptors on a Single Living Cell Surface by Scanning Electrochemical Microscopy
  • Author(s): Y. Takahashi, T. Miyamoto, H. Shiku, R. Asano, T. Yasukawa, I. Kumagai, T. Matsue
  • Journal Title: Anal. Chem. (in press)
• [Presentation] K+選択性微小電極を探針としたSECMによる固定化アルブミン近傍のK+濃度のイメージング (2010)
  • Author(s): 山田弘, 前川恒輝, 小池亨
  • Organizer: 電気化学会第77回大会
  • Place of Presentation: 富山大学五福キャンパン(富山市)
  • Year and Date: 2010-03-31
• [Presentation] Cell-based amperometric biosensor for the rapid sensing of toxicants using the leukocytes and monitoring the release of hydrogen peroxidace (2009)
  • Author(s): K.Y. Inoue, K.Ino, H.Shiku, S.Kasai, T.Yasukawa, F.Mizutani, T.Matsue
  • Organizer: 8th Asian Conference on Chemical Sensors
  • Place of Presentation: テグ(韓国)
  • Year and Date: 2009-11-11
• [Presentation] ホールセルレコーディングと微小電極法によるマクロファージ活性化過程の電気化学的評価 (2009)
  • Author(s): 高野亮介, 高橋康史, 井上久美, 伊野浩介, 珠玖仁, 末永智一
  • Organizer: 平成21年度化学系学協会東北大会
  • Place of Presentation: 日本大学工学部(郡山市)
  • Year and Date: 2009-09-19
• [Presentation] Development of multi-function probes foe gene expression analysis at single cell level (2009)
  • Author(s): 珠玖仁, Takeshi Yamakawa, Daisuke Okazaki, Genki Saito, Yasumoto Date, 伊野浩介, 末永智一
  • Organizer: The 6th International Forum On Post-Genome Technologies
  • Place of Presentation: 北京(中華民国)
  • Year and Date: 2009-09-16
• [Presentation] Use of a Surface-Modified Poly(dimethylsiloxane)Layer for the Preparation of Amperometric Glucose Sensor (2009)
  • Author(s): Tomoyuki Yasukawa, Eiji Maekawa, Yuki Yoshimura, Yuki Yoshimoto, Fumio Mizutani
  • Organizer: 60th Annual Meeting of the International Society of Electrochemistry
  • Place of Presentation: 北京(中華民国)
  • Year and Date: 2009-08-20
• [Presentation] Integrated micro/nano electrochemical systems for bioanalysis (2009)
  • Author(s): T.Matsue
  • Organizer: NIMS WEEK 2009
  • Place of Presentation: エポカルつくば
  • Year and Date: 2009-07-22
• [Presentation] Integrated micro/nano electrochemical systems for bioanalysis(Invited) (2009)
  • Author(s): T.Matsue
  • Organizer: NIMS WEEK 2009
  • Place of Presentation: エポカルつくば(つくば市)
  • Year and Date: 2009-07-22
• [Presentation] Cav channels and their modifiers : molecular identification of the binding sites. (2009)
  • Author(s): T.Yana gisawa
  • Organizer: 10th International Symposium on Mechaisms of Vasodilatation
  • Place of Presentation: 松島
  • Year and Date: 2009-06-01
• [Presentation] リング電極複合型イオンコンダクタンス顕微鏡(SICM)の開発 (2009)
  • Author(s): 村上有美、高橋康史、宮本健史、伊野浩介、珠玖仁、末永智一
  • Organizer: 第70回分析化学討論会
  • Place of Presentation: 和歌山大学栄谷キャンパス(和歌山市)
  • Year and Date: 2009-05-16
• [Presentation] Bis(benzo-15-crown-5)を用いたK^+ 選択性微小電極を探針としたSECMによる形状・局所K^+ 濃度の同時イメージング (2009)
  • Author(s): 山田弘, 神田朋宗, 小池亨
  • Organizer: 電気化学会第76回大会
  • Place of Presentation: 京都大学吉田キャンパス(京都市左京区)
  • Year and Date: 2009-03-31
• [Presentation] Single-cell analysis based on scanning probe technique and electrochemical(Invited) (2009)
  • Author(s): 珠玖仁, 末永智一
  • Organizer: 日本化学会第89春季年会
  • Place of Presentation: 日本大学理工学部船橋キャンパス(船橋市)
  • Year and Date: 2009-03-29
• [Presentation] Ca_v channels and their modifiers : molecular identification of the binding sites. (invited) (2009)
  • Author(s): Yanagisawa T.
  • Organizer: 10th MOVD 2009(10th International Symposium on Mechaisms of Vasodilatation)
  • Place of Presentation: Matsushima
• [Presentation] Electrochemical Detection of Gene Expression in a Single Living Cell (2008)
  • Author(s): T.Matsue
  • Organizer: CRC Symposium
  • Place of Presentation: 札幌
  • Year and Date: 2008-10-29
• [Presentation] Characterize Cell Function using Scanning Electrochemical Optical Microscopy with Tip-Sample Distance Regulation (2008)
  • Author(s): Y. Takahashi, T. Miyamoto, T. Murata, H. Shiku, R. Asano, T. Yasukawa, I. Kumagai, T. Matsue
  • Organizer: Pacific Rim Meeting on Electrochemical and Solid-state Science 2008
  • Place of Presentation: Honolulu (米国)
  • Year and Date: 2008-10-15
• [Presentation] INTEGRATED ELECTROCHEMICAL CELLULAR DEVICES FOR DETECTION OF GENE EXPRESSION(特別講演) (2008)
  • Author(s): T. Matsue
  • Organizer: MicroTAS 2008
  • Place of Presentation: San Diego(米国)
  • Year and Date: 2008-10-15
• [Presentation] Characterization of Cellular Functions with Scanning Electrochemical Microcopy (2008)
  • Author(s): T.Matsue
  • Organizer: Electrochemistry and Biomedical technology Symposium
  • Place of Presentation: ソウル(韓国)
  • Year and Date: 2008-10-06
• [Presentation] Characterization of Cellular Functions with Scanning Electroch emical Microcopy (2008)
  • Author(s): Tomokazu Matsue
  • Organizer: Electrochemistry and Biomedical technology Symposium
  • Place of Presentation: ソウル(韓国)
  • Year and Date: 2008-10-06
• [Presentation] Single-cell analysis of nucleic acids and proteins in gene introduction system (2008)
  • Author(s): H.Shiku, D.Okazaki, T.Yamakawa, H.Akita, H.Harashima, T.Matsue
  • Organizer: The 3 rd International Workshop on approaches to Single-Cell Analysis
  • Place of Presentation: ETH Zurich, Switzerland
  • Year and Date: 2008-09-11
• [Presentation] Single-cell analysis of nucleic acids and proteins in gene introduction system (2008)
  • Author(s): H. Shiku, D. Okazaki, T. Yam akawa, H. Akita, H. Harashima. T. Matsuue
  • Organizer: The 3^<rd> International Workshop on approach es to Single-Cell Analysis
  • Place of Presentation: ETH. Zurich, Switzerland
  • Year and Date: 2008-09-11
• [Presentation] Electrochemical Analysis of Yeast Cells Expressing Beta-Galactosidase within a Poly (dimethylsiloxane) Microwell Array (2008)
  • Author(s): H.Shiku, S.Goto, K.Nagamine, T.Yasukawa, T.Itayama, T.Matsue
  • Organizer: The 5th Workshop on Scanning Electrochemical Microscopy
  • Place of Presentation: Blue Mountain Lake (米国)
  • Year and Date: 2008-08-24
• [Presentation] Electrochemical Analysis of Yeast Cells Expressing Beta-Galactosidase within a Poly (dimethylsiloxane) Microwell Array (2008)
  • Author(s): Hitoshi Shiku, Shun Goto, Ku niaki Nagamine, Tomoyuki Yas ukawa, Tomosato Itayama, Tom okazu Matsue
  • Organizer: The 5th Workshop on Scanning Electrochemical Microscopy
  • Place of Presentation: ブルーマウンテンレイク(米国)
  • Year and Date: 2008-08-24
• [Presentation] Characteize Cell Function using Scanning Electrochemical Optical Microscopy with Tip-Sample Distance Regulation (2008)
  • Author(s): Yasufumi Takahashi, Takeshi Miyamoto, Tatsuya Murata, Hi toshii Shiku, Ryutaro Asano, Tomoyuki Yasukawa, Isumi Ku maeai. Tomokazu Matsue
  • Organizer: The 5th Workshop on Scanning Electrochemical Microscopy
  • Place of Presentation: ブルーマウンテンレイク(米国)
  • Year and Date: 2008-08-24
• [Presentation] 走査型プローブ顕微鏡による細胞初期胚機能評価 : 核酸、タンパク質、代謝レベルの定量解析(招待講演) (2008)
  • Author(s): 珠玖仁
  • Organizer: 表面・界面研究会講演会
  • Place of Presentation: 鳥栖
  • Year and Date: 2008-07-31
• [Presentation] Integrated Electrochemical Devices for Detection of Gene Expression (2008)
  • Author(s): H.Shiku
  • Organizer: 3rd German-Italian-Japanese Meeting of Electrochemists
  • Place of Presentation: Taormina, Itaria
  • Year and Date: 2008-05-25
• [Presentation] Integrated Electrochemical Devices for Detection of Gene Expre ssion(依頼講演) (2008)
  • Author(s): H. Shiku
  • Organizer: 3^<rd> German-Italian-Japanese Meeting of Electrnchemists
  • Place of Presentation: Taormina, Italy
  • Year and Date: 2008-05-25
• [Presentation] 複合酵素による走査型電気化学顕微鏡の電流シグナルの増幅と細胞膜タンパク質検出への応用 (2008)
  • Author(s): 高橋康史、宮本健史・安川智之・珠玖仁・浅野竜太郎・熊谷泉・末永智一
  • Organizer: 第69回分析化学討論会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-05-15
• [Presentation] Integrated Electrochemical Cellular Systems for Gene Engineering. (2008)
  • Author(s): T.Matsue
  • Organizer: 6th Asian Conference on Electrochemistry in Taipei
  • Place of Presentation: Taipei
  • Year and Date: 2008-05-12
• [Presentation] Integrated Electrochemical Cellular Systems for Gene Engineering (2008)
  • Author(s): T. Matsue
  • Organizer: 6th Asian Conference on Electrochemistry in Taipei (ACEC2008)
  • Place of Presentation: Taipei, Taiwan
  • Year and Date: 2008-05-12
• [Presentation] 微小イオン選択性電極を探針としたシアフォース制御走査型電気化学顕微鏡による形状・局所カリウムイオン濃度の同時測定 (2008)
  • Author(s): 山田弘,宮澤春菜,小池亨
  • Organizer: 電気化学会第75回大会
  • Place of Presentation: 山梨大学
  • Year and Date: 2008-03-31
• [Presentation] 走査型電気化学顕微鏡(SECM)を用いた上皮成長因子受容体(EGFR)のイメージング (2008)
  • Author(s): 宮本健史, 高橋康史, 珠玖仁, 浅野竜太郎, 熊谷泉, 末永智一
  • Organizer: 電気化学会第75回春季大会
  • Place of Presentation: 甲府
  • Year and Date: 2008-03-31
• [Presentation] 微小イオン選択性電極を探針としたシアフォース制御走査型電気化学顕微鏡による形状・局所カリウムイオン濃度の同時測定 (2008)
  • Author(s): 山田弘, 宮澤春菜, 小池亨
  • Organizer: 電気化学会第75回大会
  • Place of Presentation: 山梨大学(甲府市武田4)
  • Year and Date: 2008-03-31
• [Presentation] マイクログルコースセンサをプローブとした電気化学顕微鏡の開発 (2008)
  • Author(s): 安川智之, 水谷文雄
  • Organizer: 表面技術協会第117回講演大会
  • Place of Presentation: 千葉
  • Year and Date: 2008-03-12
• [Presentation] Development of SECM/SICM Hybrid System for the Study of Single Cell Surface (2007)
  • Author(s): Y. Takahashi, H. Shiku, T. Yasukawa, T. Matsue
  • Organizer: 7^<th>East Asian Conference On Chemical Sensors
  • Place of Presentation: シンガポール
  • Year and Date: 2007-12-04
• [Presentation] Electrochemical Monitoring of Gene Expression with Cellular Devices (2007)
  • Author(s): T.Matsue
  • Organizer: The 9th Asian Conferences on Analytical Sciences
  • Place of Presentation: Jeju, Korea(基調講演)
  • Year and Date: 2007-11-06
• [Presentation] Electrochemical Monitoring of Gene Expression with Cellular Devices(基調講演) (2007)
  • Author(s): T. Matsue
  • Organizer: The 9th Asian Conferences on Analytical Sciences
  • Place of Presentation: Jeju, Korea
  • Year and Date: 2007-11-06
• [Presentation] Oxvgen Consumption of Mammalian Embryos and Oocytes Monitored by Scanning Electrochemical Microscopy (2007)
  • Author(s): Hitoshi Shiku, Tomoyuki Yasukaw, Tomokazu Matsue
  • Organizer: IEEE SENSORS 2007
  • Place of Presentation: アトランタ
  • Year and Date: 2007-10-31
• [Presentation] 走査型電気化学顕微鏡(SECM)を用いた細胞表面受容体のイメージング (2007)
  • Author(s): 宮本健史, 高橋康史, 安川智之, 珠玖仁, 浅野竜太郎, 熊谷泉, 末永智一
  • Organizer: 生体機能関連化学シンポジウム
  • Place of Presentation: 仙台
  • Year and Date: 2007-09-28
• [Presentation] キャピラリー電極プローブによる単一細胞の電場破砕とmRNA定量解析 (2007)
  • Author(s): 珠玖仁, 高橋康史, 梨本裕司, 山川剛史, 安川智之, 阿部宏之, 神原秀記, 末永智一
  • Organizer: 電気化学会2007年秋季大会
  • Place of Presentation: 東京
  • Year and Date: 2007-09-20
• [Presentation] 多機能ナノ電気化学顕微鏡を用いた単一性細胞表面の膜タンパク質局所イメージング (2007)
  • Author(s): 高橋康史, 宮本健史, 安川智之, 珠玖仁, 浅野竜太郎, 熊谷泉, 末永智一
  • Organizer: 電気化学会2007年秋季大会
  • Place of Presentation: 東京
  • Year and Date: 2007-09-20
• [Presentation] Cellular Devices for Detection of Gene Expression (2007)
  • Author(s): T. Matsue
  • Organizer: The 58th Annual Meeting of the International Society of Electrochemistry
  • Place of Presentation: Banf, Canada
  • Year and Date: 2007-09-12
• [Presentation] Dual-Capillary Micro-fluidic Probe to collect mRNA from a single abherent cells (2007)
  • Author(s): H.Shiku, T.Yamakawa, Y.Nashimoto, Y.Takagasgu, Y.Torisawa, T.Yasukawa, T.Ito-Sasaki, M.Yokoo, H.Abe, H.Kambara, T.Matsue
  • Organizer: The International Forum on Post-Genome Technologies
  • Place of Presentation: 中国
  • Year and Date: 2007-09-09
• [Presentation] Dual-Capillary Micro-fluidic Probe to collect mRNA from a single abhorent cells (2007)
  • Author(s): H. Shiku, T. Yamakawa, Y. Nashimoto, Y. Takagasgu, Y. Torisawa, T. Yasukawa, T. Ito-Sasaki, M. Yokoo, H. Abe, H. Kambara, T. Matsue
  • Organizer: The International Forum on Post-Genome Technologies
  • Place of Presentation: 中国
  • Year and Date: 2007-09-09
• [Presentation] ANALYSIS OF MESSENGER RNA FROM SINGLE CELL COLLECTED USING MULTI-FUNCT IONAL PROBES (2007)
  • Author(s): Hitoshi Shiku, Takeshi Yamakawa, Yasufumi Takahashi, Yuji Nashimoto, Tomoyuki Yasukawa, Takahiro Ito-Sasaki, Masaki Yokoo, Hiroyuki Abe, Hideki Kambara, Tomokazu Matsue
  • Organizer: 日本化学会バイオテクノロジー部会
  • Place of Presentation: 東京
  • Year and Date: 2007-09-06
• [Presentation] 高感度電気化学顕微鏡を用いた細胞膜タンパク質のイメージング (2007)
  • Author(s): 高橋康史, 宮本健史, 安川智之, 珠玖仁, 浅野竜太郎, 熊谷泉, 末永智一
  • Organizer: 応用物理学会第68回学術集会
  • Place of Presentation: 札幌
  • Year and Date: 2007-09-05
• [Presentation] RECENT ADVANCES OF INTEGRATED CELLULAR DEVICES(招待講演) (2007)
  • Author(s): T. Matsue
  • Organizer: 日伊ナノテクフォーラム
  • Place of Presentation: 仙台
  • Year and Date: 2007-06-12
• [Presentation] 集積型細胞デバイスを用いた遺伝子発現の検出 (2007)
  • Author(s): 末永智一
  • Organizer: 第15回化学とマイクロシステム研究会
  • Place of Presentation: 仙台
  • Year and Date: 2007-05-25
• [Presentation] 集積型細胞デバイスを用いた遺伝子発現の検出(基調講演) (2007)
  • Author(s): 末永智一
  • Organizer: 第15回化学とマイクロシステム研究会
  • Place of Presentation: 仙台
  • Year and Date: 2007-05-25
• [Presentation] マイクログルコースセンサをプローブとした電気化学顕微鏡の開発 (2005)
  • Author(s): 安川智之,水谷文雄
  • Organizer: 表面技術協会第ll7回講演大会
  • Place of Presentation: 千葉
  • Year and Date: 2005-03-12
• [Book] 酵素活用ハンドブック「走査型電気化学顕微鏡による酵素イメージング」 (2010)
  • Author(s): 末永智一, 高橋康史, 伊野浩介, 珠玖仁
  • Publisher: エヌ・ティー・エス(印刷中)
• [Book] 化学フロンティア「切り拓き,サイエンスを牽引する分析最前線(微生物機能を利用したマイクロバイオチップの開発) (2008)
  • Author(s): 長峯邦明,末永智一
  • Publisher: 化学同人
• [Book] 先進化学センサ(細胞機能を利用するマイクロバイオセンサ) (2008)
  • Author(s): 井上(安田)久美,珠玖仁,末永智一
  • Publisher: (株)ティー・アイ・シィー
• [Book] Handbook of Bioelectrochemistry(Whole-Cell Biosensors) (2008)
  • Author(s): H. Shiku. K. Nagamine, T. Kaya, T. Yasukawa, T. Matsue
  • Publisher: John Wiley
• [Book] 先進化学センサ第II部第2章第1節「細胞機能を利用するマイクロバイオセンサ」 (2008)
  • Author(s): 井上、(安田)久美, 珠玖仁, 末永智一
  • Publisher: (株)ティー・アイ・シィー
• [Book] 化学フロンティア『切り拓き, サイエンスを牽引する分析最前線』第IV部第16章「微生物機能を利用したマイクロバイオチップの開発」 (2008)
  • Author(s): 長峯邦明, 末永智一
  • Publisher: 化学同人
• [Book] バイオセンサ・ケミカルセンサ事典(マイクロフルーイディックシステムを用いた細胞デバイス) (2007)
  • Author(s): 鳥澤勇介,安川智之,珠玖仁,末永智一
  • Publisher: テクノシステム
• [Remarks] ホームページ等
  • URL: http://www.che.tohoku.ac.jp/~bioinfo/
• [Patent(Industrial Property Rights)] 走査型電気化学イオンコンダクタンス顕微鏡測定法,走査型電気化学イオンコンダクタンス顕微鏡,その探針および探針の製造方法 (2009)
  • Inventor(s): 高橋康史,末永智一,珠玖仁,村上有美,長峯邦明,アンドリュー・シェヴチャク,ユーリ・コルチェフ
  • Industrial Property Rights Holder: 国立大学法人東北大学,インペリアル・イノベーションズ・リミティッド
  • Industrial Property Number: 2009-140602
  • Filing Date: 2009-06-11