| Budget Amount *help |
¥103,480,000 (Direct Cost: ¥79,600,000、Indirect Cost: ¥23,880,000)
Fiscal Year 2010: ¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2009: ¥19,240,000 (Direct Cost: ¥14,800,000、Indirect Cost: ¥4,440,000)
Fiscal Year 2008: ¥21,320,000 (Direct Cost: ¥16,400,000、Indirect Cost: ¥4,920,000)
Fiscal Year 2007: ¥26,650,000 (Direct Cost: ¥20,500,000、Indirect Cost: ¥6,150,000)
Fiscal Year 2006: ¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
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| Research Abstract |
We have built up the system for the efficient analysis of chromosome structure of yeast species. Based on this system, we have identified many cross talks between chromosome functions and newly developed the new system to analyze chromosome structure of higher eukaryotes. The key method is to use in vitro transcription instead of PCR for the amplification of genomic fragment from higher eukaryotes that contain many and various classes of repetitive sequences, to avoid biased amplification of repeat. Using the system we unexpectedly found that cohesion, the protein complex essential for holding sister chromatids together until the onset of M-phase, co-localizes with CTCF, the protein essential for transcriptional insulation. Our data suggest that the cohesion complex itself is required for transcriptional insulation and CTCF is required for proper localization of cohesion complexes on chromosome.
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