| Budget Amount *help |
¥107,510,000 (Direct Cost: ¥82,700,000、Indirect Cost: ¥24,810,000)
Fiscal Year 2010: ¥19,240,000 (Direct Cost: ¥14,800,000、Indirect Cost: ¥4,440,000)
Fiscal Year 2009: ¥19,240,000 (Direct Cost: ¥14,800,000、Indirect Cost: ¥4,440,000)
Fiscal Year 2008: ¥19,240,000 (Direct Cost: ¥14,800,000、Indirect Cost: ¥4,440,000)
Fiscal Year 2007: ¥21,320,000 (Direct Cost: ¥16,400,000、Indirect Cost: ¥4,920,000)
Fiscal Year 2006: ¥28,470,000 (Direct Cost: ¥21,900,000、Indirect Cost: ¥6,570,000)
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| Research Abstract |
1. We performed the structure elucidation and structure-activity relationship studies on the ComX pheromone which plays an essential role in quorum-sensing of Bacillus subtilis. We also established an in vitro system to detect the characteristic posttranslational modification on the ComX precursor to investigate the mechanisms and the universality of the modification.
2. We optimized the purification techniques to identify novel plant peptide hormones derived from putative precursor genes. Using the methods, we identified a posttranslationally modified peptide, MCLV3, which regulates the function of shoot apical meristem of Arabidopsis thaliana. In addition, we elucidated the structure of the mature form of stomagen, which is a mesophyll-derived factor controlling the stomatal density on the epidermis of Arabidopsis.
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