Modification of plant gene expression by viral gene silencing suppressors

• Project/Area Number: 18108001
• Research Category: Grant-in-Aid for Scientific Research (S)
• Allocation Type: Single-year Grants
• Research Field: Plant pathology
• Research Institution: Hokkaido University
• Principal Investigator: UYEDA Ichiro Hokkaido University, 大学院・農学研究院, 教授 (10113523)
• Co-Investigator(Kenkyū-buntansha): MASUTA Chikara 北海道大学, 大学院・農学研究院, 教授 (60281854) ; NAKAHARA Kenji 北海道大学, 大学院・農学研究院, 助教 (90315606)
• Project Period (FY): 2006 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥97,240,000 (Direct Cost: ¥74,800,000、Indirect Cost: ¥22,440,000); Fiscal Year 2010: ¥11,440,000 (Direct Cost: ¥8,800,000、Indirect Cost: ¥2,640,000); Fiscal Year 2009: ¥11,440,000 (Direct Cost: ¥8,800,000、Indirect Cost: ¥2,640,000); Fiscal Year 2008: ¥12,610,000 (Direct Cost: ¥9,700,000、Indirect Cost: ¥2,910,000); Fiscal Year 2007: ¥22,880,000 (Direct Cost: ¥17,600,000、Indirect Cost: ¥5,280,000); Fiscal Year 2006: ¥38,870,000 (Direct Cost: ¥29,900,000、Indirect Cost: ¥8,970,000)
• Keywords: 病原体相互作用 / 病原性因子 / サイレンシング / ウイルス / 植物 / 病害 / 植物ウイルス / ジーンサイレンシング

Research Abstract

Plants defense against virus infection by Post Transcriptional Gene Silencing (PTGS). We analyzed how PTGS was involved in pathogenicity and obtained the following results. (1) Suppressor activity of viral HC-Pro gene is required for induction of necrotic symptoms. (2) rgs-CaM gene has affinity to many viral suppressor genes. Its interaction destabilizes bound proteins and consequently strengthen defense against virus infection by elevating PTGS activity. (3) Y satellite RNA of cucumber mosaic virus (Y-satRNA) induces bright yellow mosaic in tobacco instead of regular green mosaic. Molecular mechanism of how Y-satRNA induces yellow mosaic is elucidated.

Research Products

• [Journal Article] A viral satellite RNA induces yellow symptoms on tobacco by targeting a gene involved in chlorophyll biosynthesis using the RNA silencing machinery. (2011)
  • Author(s): Shimura H., Pantaleo V., Ishihara T., Myojo N., Inaba J., Sueda K., Burgyan J., Masuta C.
  • Journal Title: PLoS Pathogens (in press)
  • NAID: 120003059662
  • Peer Reviewed
• [Journal Article] Virus induced gene silencing in Antirrhinum majus using the Cucumber mosaic virus vector : Functional analysis of the AINTEGUMENTA (Am-ANT) gene of A.majus. (2011)
  • Author(s): Kim B.M., Inaba J., Masuta C.
  • Journal Title: Horticulture, Environment, and Biotechnology 52(in press)
  • Peer Reviewed
• [Journal Article] Virus induced gene silencing in Antirrhinum majus using the Cucumber mosaic virus vector : Functional analysis of the AINTEGUMENTA (Am-ANT) gene of A. majus. (2011)
  • Author(s): Kim, B.M.
  • Journal Title: Horticulture, Environment, and Biotechnology Volume: 52(in press)
  • Peer Reviewed
• [Journal Article] Sensitive PCR-based detection of Apple chlorotic leaf spot virus heterog enous in apple trees (2011)
  • Author(s): Nakahara, K.
  • Journal Title: Japan Agricultural Research Quarterly Volume: (in press)
  • Peer Reviewed
• [Journal Article] HC-Pro, a potyvirus RNA silencing suppressor, cancels cycling of Cucumber mosaic virus in Nicotiana benthamiana plants. (2010)
  • Author(s): Fukuzawa N., Itchoda N., Ishihara T., Goto K., Masuta C., Matsumura T.
  • Journal Title: Virus Genes 40 Pages: 440-446
  • Peer Reviewed
• [Journal Article] The C-terminal residues of the 2b protein of Cucumber mosaic virus are important for efficient expression in Escherichia coli and DNA binding. (2010)
  • Author(s): Sueda K., Shimura H., Meguro A., Uchida T., Inaba J., Masuta C.
  • Journal Title: FEBS Letters 584 Pages: 945-950
  • Peer Reviewed
• [Journal Article] A progeny virus from a cucumovirus pseudorecombinant evolved to gain the ability to accumulate its RNA silencing suppressor leading to systemic infection in tobacco. (2010)
  • Author(s): Asaoka R., Shimura H., Arai M., Masuta C.
  • Journal Title: Molecular Plant-Microbe Interactions 23 Pages: 32-339
  • Peer Reviewed
• [Journal Article] The P3 Protein of turnip mosaic virus can alone induce HR-like cell death in Arabidopsis thaliana carrying TuNI. (2010)
  • Author(s): Kim B., Suehiro N., Natsuaki T., Inukai T., Masuta C.
  • Journal Title: Molecular Plant-Microbe Interactions 40 Pages: 144-152
  • Peer Reviewed
• [Journal Article] Involvement of the P1 cistron in overcoming eIF4E-mediated recessive resistance against Clover yellow vein virus in pea. (2010)
  • Author(s): Nakahara K.S., Shimada R., Choi H.S., Yamamoto H., Jun S., Uyeda I.
  • Journal Title: Moleular Plant Microbe Interactions 23 Pages: 1460-1469
  • Peer Reviewed
• [Journal Article] The P3 Protein of turnip mosaic virus can alone induce HR-like cell death in Arabidopsis thaliana carrying TuNI. (2010)
  • Author(s): Kim, B.
  • Journal Title: Molecular Plant-Microbe Interactions Volume: 40 Pages: 144-152
  • Peer Reviewed
• [Journal Article] Mixed Infection in Tomato to Ensure Frequent Generation of A Natural Reassortant Between Two Subgroups of Cucumber mosaic virus. (2010)
  • Author(s): Maoka, T.
  • Journal Title: Virus Genes Volume: 40 Pages: 148-150
  • Peer Reviewed
• [Journal Article] A progeny virus from a cucumovirus pseudorecombinant evolved to gain the ability to accumulate its RNA silencing suppre ssor leading to systemic infection in tobacco. (2010)
  • Author(s): Asaoka, R.
  • Journal Title: Molecular Plant-Microbe Interactions Volume: 23 Pages: 32-339
  • Peer Reviewed
• [Journal Article] The C-terminal residues of the 2b protein of Cucumber mosaic virus are important for efficient expression in Escherichia coli and DNA binding. (2010)
  • Author(s): Sueda, K.
  • Journal Title: FEBS Letters Volume: 584 Pages: 945-950
  • Peer Reviewed
• [Journal Article] HC-Pro, a potyvirus RNA silencing suppressor, cancels cycling of Cucumber mosaic virus in Nicotiana benthamiana plants. (2010)
  • Author(s): Fukuzawa, N.
  • Journal Title: Virus Genes Volume: 40 Pages: 440-446
  • Peer Reviewed
• [Journal Article] Soybean stem growth habit gene Dt1 is an orthologue of Ara bidopsis TFL1. (2010)
  • Author(s): Liu, B.
  • Journal Title: Plant Physiology Volume: 153 Pages: 198-210
  • Peer Reviewed
• [Journal Article] Risk-managed production of bioactive recombinant proteins using a novel plant virus vector with a helper plant to comple ment viral systemic movement. (2010)
  • Author(s): Fukuzawa, N.
  • Journal Title: Plant Biotechnology Journal Volume: (in press)
  • Peer Reviewed
• [Journal Article] Resistant and Susceptible Responses in Tomato to Cyst Nema tode are Differentially Regulated by Salicylic Acid. (2010)
  • Author(s): Uehara, T.
  • Journal Title: Plant Cell Physiology Volume: 51 Pages: 1524-1536
  • Peer Reviewed
• [Journal Article] Virus-mediated efficient induction of epigenetic modifications of endogenous genes with phenotypic changes in plants. (2010)
  • Author(s): Kanazawa, A.
  • Journal Title: The Plant Journal Volume: 65 Pages: 156-168
  • Peer Reviewed
• [Journal Article] Involvement of the P1 Cistron in Overcoming eIF4E-Mediated Recessive Resistance Against Clover yellow vein virus in Pea (2010)
  • Author(s): Nakahara, K.
  • Journal Title: Molecular Plant Microbe Interactions Volume: 23 Pages: 1460-1469
  • Peer Reviewed
• [Journal Article] The P3 Protein of turnip mosaic virus can alone induce HR-like cell death in Arabidopsis thaliana carrying TuNI. (2010)
  • Author(s): Kim, B.
  • Journal Title: Molecular Plant-Microbe Interactions 40 Pages: 144-152
  • Peer Reviewed
• [Journal Article] Mixed Infection in Tomato to Ensure Frequent Generation of A Natural Reassortant Between Two Subgroups of Cucumber mosaic virus. (2010)
  • Author(s): Maoka, T.
  • Journal Title: Virus Genes 40 Pages: 148-150
  • Peer Reviewed
• [Journal Article] A progeny virus from a cucumovirus pseudorecombinant evolved to gain the ability to accumulate its RNA silencing suppressor leading to systemic infection in tobacco. (2010)
  • Author(s): Asaoka, R.
  • Journal Title: Molecular Plant-Microbe Interactions 23 Pages: 32-339
  • Peer Reviewed
• [Journal Article] The C-terminal residues of the 2b protein of Cucumber mosaic virus are important for efficient expression in Escherichia coli and DNA binding. (2010)
  • Author(s): Sueda, K.
  • Journal Title: FEBS Letters 584 Pages: 945-950
  • Peer Reviewed
• [Journal Article] Silencing by small RNAs is linked to endosomal trafficking (2009)
  • Author(s): Lee Y.S., Pressman S., Andress A.P., Kim K., White J.L., Cassidy J.J., Li1 X., Lubel1 K., Lim D.H., Cho I.S., Nakahara K., Preall1 J. B., Bellarel P., Sontheimer1 E.J., Carthew R.W.
  • Journal Title: Nature Cell Biology 11 Pages: 1150-1156
  • Peer Reviewed
• [Journal Article] Eukaryotic initiation vactor 4E controls cyv 2 resistance in pea. (2009)
  • Author(s): Andrade M., Abe Y., Nakahara K. S., Uyeda I.
  • Journal Title: J Gen Plant Pathol. 75 Pages: 241-249
• [Journal Article] Activation of the salicylic acid signaling pathway enhances Clover yellow vein virus virulence in susceptible pea cultivars. (2009)
  • Author(s): Atsumi G., Kagaya U., Kitazawa H., Nakahara K.S., Uyeda I.
  • Journal Title: Moleular Plant Microbe Interactions 22 Pages: 166-175
• [Journal Article] Silencing by small RNAs is linked to endosomal trafficking. (2009)
  • Author(s): Lee, Y.S.
  • Journal Title: Nat Cell Biol 11 Pages: 1150-1156
  • Peer Reviewed
• [Journal Article] Activation of the salicylic acid signaling pathway enhances Clover yellow vein virus virulence in susceptible pea cultivars. (2009)
  • Author(s): Atsumi, G.
  • Journal Title: Mol Plant Mierobe Interactions 22 Pages: 166-175
  • Peer Reviewed
• [Journal Article] The cyv-2 resistance to Clover yellow vein virus in pea is controlled by the eukaryotic initiation factor 4E. (2009)
  • Author(s): Andrade, M.
  • Journal Title: J Gen Plant Pathol 75 Pages: 241-249
  • Peer Reviewed
• [Journal Article] Simultaneous UPLC MS/MS analysis of endogenous jasmonic acid, salicylic acid, and their related compounds. (2009)
  • Author(s): Matuura, H.
  • Journal Title: Plant Growth Regulation 57 Pages: 293-301
  • Peer Reviewed
• [Journal Article] Purification and cDNA cloning of a wound inducible glucosyltransferase active toward 12-hydroxy jasmonic acid. (2009)
  • Author(s): Seto, Y.
  • Journal Title: Phytochemistry 70 Pages: 370-379
  • Peer Reviewed
• [Journal Article] Activation of the salicylic acid signaling pathway enhances Clover yellow vein virus virulence in susceptible pea cultivars (2009)
  • Author(s): Atsumi G., Kagaya U., Kitazawa H., Nakahara K. S., Uyeda I.
  • Journal Title: Moleular Plant Microbe Interactions 22 Pages: 166-175
  • Peer Reviewed
• [Journal Article] Activation of the salicylic acid signaling pathway enhances Clover yellow vein virus virulence in susceptible pea cultivars (2009)
  • Author(s): Atsumi, G.
  • Journal Title: Molecular Plant Microbe Interactions 22 Pages: 166-175
  • Peer Reviewed
• [Journal Article] A strategy for screening an inhibitor of viral silencing suppressors, which attenuates symptom development of plant viruses. (2008)
  • Author(s): Shimura H., Fukagawa T., Meguro A., Yamada H., Oh-hira M., Sano S., Masuta C.
  • Journal Title: FEBS Letters 582 Pages: 4047-4052
  • Peer Reviewed
• [Journal Article] Down-regulation of flavonoid 3'-hydroxylase gene expression by virus-induced gene silencing in soybean reveals the presence of a threshold mRNA level associated with pigmentation in pubescence. (2008)
  • Author(s): Nagamatsu A., Masuta C., Matsuura H., Kitamura K., Abe J., Kanazawa A.
  • Journal Title: Journal of Plant Physiology 5 Pages: 778-790
  • Peer Reviewed
• [Journal Article] Degree of RNA silencing and ability of a viral suppressor vary depending on the cell species in a protoplast system. (2008)
  • Author(s): Shimura H., Kogure Y., Goto K., Masuta C.
  • Journal Title: Journal of General Plant Pathology 74 Pages: 326-330
  • NAID: 10029716605
  • Peer Reviewed
• [Journal Article] Point mutations in helper component protease of clover yellow vein virus are associated with the attenuation of RNA-silencing suppression activity and symptom expression in broad bean. (2008)
  • Author(s): Yambao M.L.M., Yagihashi H., Sekiguchi H., Sekiguchi T., Sasaki T., Sato M., Atsumi G., Tacahashi Y., Nakahara K.S., Uyeda I.
  • Journal Title: Archives Virology 153 Pages: 105-115
  • Peer Reviewed
• [Journal Article] Point mutations in helper component protease of clover yellow vein virus are associated with the attenuation of RNA-silencing suppression activity and symptom expression in broad bean (2008)
  • Author(s): Yambao M.L.M., Yagihashi H., Sekiguchi H., Sekiguchi T., Sasaki T., Sato M., Atsumi G., Tacahashi Y., Nakahara K. S., Uyeda I.
  • Journal Title: Arch. Virol. 153 Pages: 105-115
  • Peer Reviewed
• [Journal Article] Degree of RNA silencing and ability of a viral suppressor vary depending on the cell species in a protoplast system (2008)
  • Author(s): Shimura, H., Kogure, Y., Goto, K. and Masuta, C.
  • Journal Title: Journal of General Plant Pathology 74 Pages: 326-330
  • NAID: 10029716605
  • Peer Reviewed
• [Journal Article] A strategy for screening an inhibitor of viral silencing suppressors, which attenuates symptom development of plant viruses (2008)
  • Author(s): Shimura, H., Fukagawa, T., Meguro, A., Yamada, H., Oh-hira, M., Sano, S. and Masuta C.
  • Journal Title: FEBS Letters Pages: 4047-4052
  • Peer Reviewed
• [Journal Article] A strategy for screening an inhibitor of viral silencing suppressors, which attenuates symptom development of plant viruses (2008)
  • Author(s): Shimura, H.
  • Journal Title: FEBS Letters 582 Pages: 4047-4052
  • Peer Reviewed
• [Journal Article] A simple, sensitive, specific detection of mixed infection of multiple plant viruses using macroarray and microtube hybridization (2008)
  • Author(s): Sugiyama, S.
  • Journal Title: Journal of Virogical Methods 153 Pages: 241-244
  • Peer Reviewed
• [Journal Article] Point mutations in helper component protease of clover yellow vein virus are associated with the attenuation of RNA-silencing suppression activity and symptom expression in broad bean (2008)
  • Author(s): M. L. M. Yambao
  • Journal Title: Archives of Virology 153 Pages: 105-115
  • Peer Reviewed
• [Journal Article] Veinal necrosis induced by Turnip mosaic virus infection in Arabidopsis is a form of defense response accompanying HR-like cell death. (2008)
  • Author(s): B. Kim
  • Journal Title: Molcular Plant Microbe Interactions 21 Pages: 260-268
  • Peer Reviewed
• [Journal Article] Degree of RNA silencing andabilityof a viral suppressor vary depending on the cell species in a protoplast system. Journal of General Plant Pathology (2008)
  • Author(s): H. Shimura
  • Journal Title: Journal of General Plant Pathology (in press)
  • Peer Reviewed
• [Journal Article] Down-regulation of flavonoid 3'-hydroxylase gene expression by virus-induced gene silencing in soybean reveals the presence of a threshold mRNA level associated with pigmentation in pubescence (2008)
  • Author(s): A. Nagamatsu
  • Journal Title: Journal of Plant Physiology (in press)
  • Peer Reviewed
• [Journal Article] A.Functional analysis of soybean genes involved in flavonoid biosynthesis by virus-induced gene silencing. (2007)
  • Author(s): Nagamatsu A., Masuta C., Senda M., Matsuura H., Kasai A., Hong J.-S., Kitamura K., Abe J., Kanazawa A.
  • Journal Title: Plant Biotechnology Journal 5 Pages: 778-90
  • Peer Reviewed
• [Journal Article] Charactrization of silencing suppressor 2b of Cucumber mosaic virus based on examination of its small RNA-binding abilities. (2007)
  • Author(s): Goto K., Kobori T., Kosaka Y., Natsuaki T., Masuta C.
  • Journal Title: Plant Cell Physiology 48 Pages: 1050-1060
  • Peer Reviewed
• [Journal Article] Genetic analysis of lethal tip necrosis induced by Clover yellow vein virus infection in pea. (2007)
  • Author(s): Ravelo G., Kagaya U., Inukai T., Sato M., Uyeda I.
  • Journal Title: J.Gen.Plant Pathol. 73 Pages: 59-65
  • Peer Reviewed
• [Journal Article] Two resistance modes to Clover yellow vein virus in pea characterized by a green fluorescent protein-tagged virus. (2007)
  • Author(s): Andrade M., Sato M., Uyeda I.
  • Journal Title: Phytoathology 97 Pages: 544-550
  • Peer Reviewed
• [Journal Article] Development of Cucumber mosaic virus as a vector modifiable for different host species to produce therapeutic proteins. (2007)
  • Author(s): Matsuo K., Hong J.-S., Tabayashi N., Ito A., Masuta C., Matsumura T.
  • Journal Title: Planta 225 Pages: 277-286
  • Peer Reviewed
• [Journal Article] Two Resistance Modes to Clover yellow vein virus in Pea Characterized by a Green Fluorescent Protein-Tagged Virus (2007)
  • Author(s): M. Andrade
  • Journal Title: Phytopathology 97 Pages: 544-550
  • Peer Reviewed
• [Journal Article] Comparative serial analysis of gene expression (SAGE) of transcript profiles of tomato roots infected with cyst nematode (2007)
  • Author(s): T. Uehara
  • Journal Title: Plant Molecular Biology 63 Pages: 185-194
  • Peer Reviewed
• [Journal Article] Infection of Cucumber mosaic virus to soybean as determined by viral movementprotein. (2007)
  • Author(s): J. S. Hong
  • Journal Title: Archives of Virology 152 Pages: 321-328
  • Peer Reviewed
• [Journal Article] Charactrization of silencing suppressor 2b of Cucumber mosaic virus based on examination of its small RNA-binding abilities (2007)
  • Author(s): K. Goto
  • Journal Title: Plant and Cell Physiology 48 Pages: 1050-1060
  • Peer Reviewed
• [Journal Article] Functional analysis of soybean genes involved in flavonoid biosynthesis by virusinduced gene silencing. (2007)
  • Author(s): A. Nagamatsu
  • Journal Title: Plant Biotechnology Journal 5 Pages: 778-790
  • Peer Reviewed
• [Journal Article] 総説 ジーンサイレンシングによる植物のウイルス抵抗性 (2007)
  • Author(s): 増田 税
  • Journal Title: BRAINテクノニュース 121 Pages: 14-17
• [Journal Article] 総説 植物のウイルスに対する防御機構 (2007)
  • Author(s): 増田 税
  • Journal Title: 蛋白質核酸酵素 Pages: 1221-1226
• [Journal Article] Genetic analysis of lethal tip necrosis induced by Clover yellow vei virus infection in pea (2007)
  • Author(s): G.Ravelo, U.Kagaya, T.Inukai, M.Sato, I.Uyeda
  • Journal Title: Journal of General Plant Pathology 73 Pages: 59-65
• [Journal Article] Two resitance modes to Clover yellow vein virus in pea characterized by a green-fluorescent-protein-tagged virus (2007)
  • Author(s): M.Andrade, M.Sato, I.Uyeda
  • Journal Title: Phytopathology 97(In press)
• [Journal Article] Development of Cucumber mosaic virus as a vector modifiable for different host species to produce therapeutic proteins (2007)
  • Author(s): K.Matsuo, J.-S.Hong, N.Tabayashi, A Ito, C.Masuta, T.Matsumura
  • Journal Title: Planta 225 Pages: 277-286
• [Journal Article] Rapid induction of transcriptional and post-transcriptional gene silencing using a novel Cucumber mosaic virus vector. (2006)
  • Author(s): Otagaki S., Arai M., Takahashi A., Goto K., Hong J.-S., Masuta C., Kanazawa, A.
  • Journal Title: Plant Biotechnology 23 Pages: 259-265
  • NAID: 10021908671
  • Peer Reviewed
• [Journal Article] Rapid induction of transcriptional and post-transcriptional gene silencing using a novel Cucumber mosaic virus vector (2006)
  • Author(s): S.Otagaki, M.Arai, A.Takahashi, K.Goto, J.-S.Hong, C.Masuta, A.Kanazawa
  • Journal Title: Plant Biotechnology 23 Pages: 259-265
  • NAID: 10021908671
• [Presentation] エピジェネティックな変化を誘導することで遺伝子組換えによらずに形質を変化させた植物体の作出 (2011)
  • Author(s): 金澤章
  • Organizer: 日本育種学会第119回講演会
  • Place of Presentation: 横浜市立大学(横浜市)
  • Year and Date: 2011-03-30
• [Presentation] タバコのウイルスに対する基礎免疫におけるカルモジュリン様タンパクrgs-CaMの役割1 (2011)
  • Author(s): 中原健二
  • Organizer: 平成23年度日本植物病理学会大会
  • Place of Presentation: 東京農工大学(府中市)
  • Year and Date: 2011-03-29
• [Presentation] キュウリモザイクウイルスYサテライトRNAが誘導する黄化症状の分子メカニズムの解析 (2011)
  • Author(s): 志村華子、増田税, 他
  • Organizer: 平成23年度日本植物病理学会大会
  • Place of Presentation: 府中市、東京農工大学
  • Year and Date: 2011-03-27
• [Presentation] ニンニク鱗茎からの効率的なウイルス検出の確立 (2011)
  • Author(s): 吉田直人
  • Organizer: 平成23年度日本植物病理学会大会
  • Place of Presentation: 東京農工大学(府中市)
  • Year and Date: 2011-03-27
• [Presentation] 感染葉におけるキュウリモザイクウイルス2bタンパク質のリン酸修飾解析 (2011)
  • Author(s): 末田香恵
  • Organizer: 平成23年度日本植物病理学会大会
  • Place of Presentation: 東京農工大学(府中市)
  • Year and Date: 2011-03-27
• [Presentation] Brassica rapaにおけるTuMVに対するえそ誘導遺伝子座Rnt1のマッピング (2011)
  • Author(s): 藤原綾香
  • Organizer: 平成23年度日本植物病理学会大会
  • Place of Presentation: 東京農工大学(府中市)
  • Year and Date: 2011-03-27
• [Presentation] キュウリモザイクウイルスYサテライトRNAが誘導する黄化症状の分子メカニズムの解析 (2011)
  • Author(s): 志村華子
  • Organizer: 平成23年度日本植物病理学会大会
  • Place of Presentation: 東京農工大学(府中市)
  • Year and Date: 2011-03-27
• [Presentation] Genome analyses of P3 and P3N-PIPO of Clover yellow vein virus that breaks resistance in tentative cyv1 pea (2011)
  • Author(s): Choi, S.
  • Organizer: 平成23年度日本植物病理学会大会
  • Place of Presentation: 東京農工大学(府中市)
  • Year and Date: 2011-03-27
• [Presentation] he inheritable transcriptional gene silencing targeted to endogenous genes in petunia by usine the Cucumber mosaic virus vector. (2011)
  • Author(s): Inaba, J.
  • Organizer: Keystone Symposia on Molecular and Cellular Biology
  • Place of Presentation: Monterey, California, USA
  • Year and Date: 2011-03-22
• [Presentation] Counterdefense against Viral RNAi Suppressors in Tobacco. (2011)
  • Author(s): 中原健二, 他
  • Organizer: Keystone Symposia on Molecular and Cellular Biology
  • Place of Presentation: Monterey, California, USA
• [Presentation] Counterdefense against Viral RNAi Suppressors in Tobacco (2011)
  • Author(s): Nakahara, K.
  • Organizer: Keystone SYMPOSIA Mechanism and Biology of Silencing
  • Place of Presentation: Monterey, California, USA 招待講演
• [Presentation] Screening of antiviral agents to inhibit RNA silencing suppressors of plant and animal viruses. (2010)
  • Author(s): Fujiwara A., Masuta C., 他
  • Organizer: Antivirals Congress
  • Place of Presentation: Amsterdam, Nederland
  • Year and Date: 2010-11-07
• [Presentation] Screening of antiviral agents to inhibit RNA silencing supressors of plant and animal viruses. (2010)
  • Author(s): Fujiwara, A.
  • Organizer: Antivirals Congress
  • Place of Presentation: Amsterdam, Nederland
  • Year and Date: 2010-11-07
• [Presentation] Effect of rgs-CaM overexpression on PVY accumulation in transgenic tobacco plants. (2010)
  • Author(s): Yamada S., Nakahara K., Uyeda I. 他
  • Organizer: Keystone symposia (RNA silencing mechanisms inplants)
  • Place of Presentation: Santa Fe, New Mexico, USA
• [Presentation] Effect of rgs-CaM overexpression on PVY accumulation in transgenic to bacco plants (2010)
  • Author(s): Yamada, S.
  • Organizer: Keystone symposia(RNA silencing mechanisms in plants)
  • Place of Presentation: Santa Fe, New Mexico, USA
• [Presentation] 植物ウイルスベクターを介して誘導される外来性遺伝子のプロモーター領域のメチル化状態と転写型ジーンサイレンシングの安定性 (2009)
  • Author(s): 太田垣駿吾
  • Organizer: 日本育種学会第116回講演会
  • Place of Presentation: 北海道大学
  • Year and Date: 2009-10-26
• [Presentation] カブモザイクウイルスに感染したハクサイにおける病徴決定因子 (2009)
  • Author(s): 藤原綾香
  • Organizer: 平成21年度植物感染生理談話会
  • Place of Presentation: 大沼国際セミナーハウス(函館)
  • Year and Date: 2009-08-07
• [Presentation] キュウリモザイクウイルスベクターに挿入する配列の長さと外来性遺伝子の効率的な転写不活性化誘導の関連性 (2009)
  • Author(s): 太田垣駿吾
  • Organizer: 平成21年度植物感染生理談話会
  • Place of Presentation: 大沼国際セミナーハウス(函館)
  • Year and Date: 2009-08-07
• [Presentation] キュウリモザイクウイルスによって誘導されたペチュニア内在性遺伝子のプロモーター領域のメチル化とヒストン修飾の解析 (2009)
  • Author(s): 稲場純一
  • Organizer: 平成21年度植物感染生理談話会
  • Place of Presentation: 大沼国際セミナーハウス(函館)
  • Year and Date: 2009-08-07
• [Presentation] キュウリモザイクウイルスサテライトRNA が植物のRNA サイレンシングへ及ぼす影響の解析 (2009)
  • Author(s): 志村華子
  • Organizer: 日本植物病理学会学会
  • Place of Presentation: 山形大学
  • Year and Date: 2009-03-27
• [Presentation] Cucumber mosaic virus vector has the ability to induce inheritable transcriptional gene silencing of endogenes in petunia. (2009)
  • Author(s): 稲場純一、増田税, 他
  • Organizer: The 1st Japan-Korea joint symposium
  • Place of Presentation: Jeju, Korea
• [Presentation] Two separated regions in virus genome contribute to high virulence expression of Clover yellow vein virus 90-1 Br-2 isolate in peas. (2009)
  • Author(s): Atsumi G., Nakahara K., Uyeda I. 他
  • Organizer: The 1st Japan-Korea joint symposium
  • Place of Presentation: Jeju, Korea
• [Presentation] Comparison of the 2b proteins from two isolates of Tomato aspermy virus in silencing suppressor activity and viral systemic movement. (2009)
  • Author(s): Shimura H., Masuta C. 他
  • Organizer: International Congress of Virology, The INTERNATIONAL UNION OF MICROBIOLOGICAL SOCIETIES
  • Place of Presentation: Istanbul, ISTANBUL CONVENTION AND EXHIBITION CENTRE
• [Presentation] キュウリモザイクウイルスサテライトRNAが植物のRNAサイレンシングへ及ぼす影響の解析 (2009)
  • Author(s): 志村華子、増田税, 他
  • Organizer: 平成21年度日本植物病理学会大会
  • Place of Presentation: 山形大学小白川キャンパス
• [Presentation] サブグループIとIIのキュウリモザイクウイルスの2bタンパク質のサイレンシング抑制活性の比較 (2009)
  • Author(s): 後藤一法、増田税, 他
  • Organizer: 平成21年度日本植物病理学会大会
  • Place of Presentation: 山形大学小白川キャンパス
• [Presentation] キュウリモザイクウイルスサテライトRNAが植物のRNAサイレンシングへ及ぼす影響の解析 (2009)
  • Author(s): 志村華子、増田税, 他
  • Organizer: 平成21年度日本植物病理学会大会
  • Place of Presentation: 山形市、山形大学
• [Presentation] サブグループIとIIのキュウリモザイクウイルスの2bタンパク質のサイレンシング抑制活性の比較 (2009)
  • Author(s): 後藤一法、増田税, 他
  • Organizer: 平成21年度日本植物病理学会大会
  • Place of Presentation: 山形市、山形大学
• [Presentation] エンドウPI 226564系統においてクローバ葉脈黄化ウイルスの強毒性を決定するウイルス因子の探索 (2009)
  • Author(s): 厚見剛、中原健二、上田一郎, 他
  • Organizer: 平成21年度日本植物病理学会大会
  • Place of Presentation: 山形市、山形大学
• [Presentation] クローバ葉脈黄化ウイルスはP1に点変異を生じることにより翻訳開始因子をコードするcyv-2遺伝子によるエンドウ劣性抵抗性を打破する (2009)
  • Author(s): 中原健二、上田一郎, 他
  • Organizer: 平成21年度日本植物病理学会大会
  • Place of Presentation: 山形市、山形大学
• [Presentation] Viral non-proliferation system to produce a valuable protein in transgenic plants. (2009)
  • Author(s): 福澤徳穂
  • Organizer: 3th Internalional Conference of Plant-Based Vaccine & Antibody
  • Place of Presentation: ヴェローナ(イタリア)
• [Presentation] CMVベクターによる高効率な物質生産が可能な植物体の作出とサイクリックなCMV蓄積への影響 (2009)
  • Author(s): 石原岳明
  • Organizer: 平成21年度日本植物病理学会大会北海道部会
  • Place of Presentation: 北海道大学
• [Presentation] サブグループ識別(SS)-RT-PCRによるリアソータントCMVの検出 (2009)
  • Author(s): 眞岡哲夫
  • Organizer: 平成21年度日本植物病理学会大会北海道部会
  • Place of Presentation: 北海道大学
• [Presentation] オオムギいもち病抵抗性の遺伝 (2009)
  • Author(s): 犬飼剛
  • Organizer: 平成21年度日本植物病理学会北海道部会談話会
  • Place of Presentation: 北海道大学
• [Presentation] Cucumber mosaic virus vector has the ability to induce inheritable transcriptional gene silencing of endogenes in petunia. (2009)
  • Author(s): 稲場純一
  • Organizer: The 1^<st> Japan-Korea joint symposium
  • Place of Presentation: Jeju, Korea
• [Presentation] The P3 of Turnip mosaic virus is an avirulence determinant for TuNI in Arabidopsis thaliana to induce HR-like cell death. (2009)
  • Author(s): キム・ボミン
  • Organizer: The 1^<st> Japan-Korea joint symposium
  • Place of Presentation: Jeju, Korea
• [Presentation] Two separated regions in virus genome contribute to high virulence expression of Clover yellow vein virus 90-1 Br-2 isolate in peas (2009)
  • Author(s): Atsumi, G.
  • Organizer: The 1^<st> Japan-Korea joint symposium
  • Place of Presentation: Jeju, Korea
• [Presentation] A resistance gene in pea line PI429853 to Clover yellow vein virus in mapped to linkage group II and linked with eyv1 (2009)
  • Author(s): Choi, S.-H.
  • Organizer: The 1^<st> Japan-Korea joint symposium
  • Place of Presentation: Jeju, Korea
• [Presentation] Analysis of pea proteome during Clover yellow vein virus infection (2009)
  • Author(s): Yamada, S.
  • Organizer: The 1^<st> Japan-Korea joint symposium
  • Place of Presentation: Jeju, Korea
• [Presentation] ウイルス感染時における新規宿主因子XYPPX repeat遺伝子の機能解析 (2009)
  • Author(s): 厚見剛
  • Organizer: 第50回日本植物生理学会
  • Place of Presentation: 名古屋市、名古屋大学
• [Presentation] RNAウイルス間RNA組換えの回避戦略 (2009)
  • Author(s): 石原岳明
  • Organizer: 平成21年度日本植物病理学会大会
  • Place of Presentation: 山形市、山形大学
• [Presentation] キュウリモザイクウイルスサテライトRNAが植物のRNAサイレンシングヘ及ぼす影響の解析 (2009)
  • Author(s): 志村華子
  • Organizer: 平成21年度日本植物病理学会大会
  • Place of Presentation: 山形市、山形大学
• [Presentation] 作物健康センサーによる土壌病害虫検出法の開発 (2009)
  • Author(s): 杉山俊平
  • Organizer: 平成21年度日本植物病理学会大会
  • Place of Presentation: 山形市、山形大学
• [Presentation] キュウリモザイクウイルスY2bタンパク質が大腸菌毒性を示す要因の決定 (2009)
  • Author(s): 末田香恵
  • Organizer: 平成21年度日本植物病理学会大会
  • Place of Presentation: 山形市、山形大学
• [Presentation] カブモザイクウイルスに感染したハクサイの病徴を決定する宿主及びウイルス因子 (2009)
  • Author(s): 藤原綾香
  • Organizer: 平成21年度日本植物病理学会大会
  • Place of Presentation: 山形市、山形大学
• [Presentation] バナナから分離されたキュウリモザイクウイルスの全塩基配列の決定 (2009)
  • Author(s): 松澤章彦
  • Organizer: 平成21年度日本植物病理学会大会
  • Place of Presentation: 山形市、山形大学
• [Presentation] いもち病菌に感染したオオムギにおいて誘導される防御関連遺伝子のe QTL (2009)
  • Author(s): 犬飼剛
  • Organizer: 平成21年度日本植物病理学会大会
  • Place of Presentation: 山形市、山形大学
• [Presentation] RNAサイレンシングは植物組織の着色に必要なフラボノイド合成系遺伝子のmRNA量の閾値を明らかにする (2008)
  • Author(s): 永松敦、増田税, 他
  • Organizer: 日本分子生物学会第31回年会
  • Place of Presentation: 神戸市、神戸ポートアイランド
  • Year and Date: 2008-12-12
• [Presentation] RNAサイレンシングは植物組織の着色に必要なフラボノイド合成系遺伝子のm RNA量の閾値を明らかにする (2008)
  • Author(s): 永松敦
  • Organizer: 日本分子生物学会第31回年会
  • Place of Presentation: 神戸市神戸ポートアイランド
  • Year and Date: 2008-12-12
• [Presentation] 植物ウイルスベクターを介した外来性プロモーターへのメチル化の誘導はDNA脱メチル化酵素遺伝子の発現抑制により促進される (2008)
  • Author(s): 太田垣駿吾
  • Organizer: 日本分子生物学会第31回年会
  • Place of Presentation: 神戸市神戸ポートアイランド
  • Year and Date: 2008-12-12
• [Presentation] 植物におけるウイルスベクターを用いたRNA-directed DNA methylationにより誘導される転写不活性化において誘導源配列の10 bpの長さの違いが不活性化効率に大きく影響する (2008)
  • Author(s): 河合文珠
  • Organizer: 日本分子生物学会第31回年会
  • Place of Presentation: 神戸市神戸ポートアイランド
  • Year and Date: 2008-12-12
• [Presentation] 植物ウイルスベクターを用いた遺伝子特異的な転写不活性化におけるベクター挿入配列の違いに依存した不活性化効率の変化 (2008)
  • Author(s): 河合文珠
  • Organizer: 札幌農林学会平成20年度大会
  • Place of Presentation: 札幌市、北海道大学
  • Year and Date: 2008-12-06
• [Presentation] クローバ葉脈黄化ウイルスの感染で誘導されるエンドウ遺伝子の発現 (2008)
  • Author(s): 山田翔太、中原健二、上田一郎, 他
  • Organizer: 平成20年度日本植物病理学会大会北海道部会
  • Place of Presentation: 札幌市、北海道農業研究センター
  • Year and Date: 2008-10-17
• [Presentation] キュウリモザイクウイルス2b変異タンパク質の大腸菌及び植物での発現解析 (2008)
  • Author(s): 末田香恵
  • Organizer: 平成20年度日本植物病理学会大会北海道部会
  • Place of Presentation: 札幌市、北海道農業研究センター
  • Year and Date: 2008-10-17
• [Presentation] アラビドプシスのプロトプラストアッセイ系を用いたカブモザイクウイルスに誘導されるえそ病徴誘導因子,TuNIの同定 (2008)
  • Author(s): 金甫民
  • Organizer: 平成20年度日本植物病理学会大会北海道部会
  • Place of Presentation: 札幌市、北海道農業研究センター
  • Year and Date: 2008-10-17
• [Presentation] バナナから分離されたキュウリモザイクウイルスの塩基配列の解析 (2008)
  • Author(s): 松澤章彦
  • Organizer: 平成20年度日本植物病理学会大会北海道部
  • Place of Presentation: 札幌市、北海道農業研究センター
  • Year and Date: 2008-10-17
• [Presentation] カブモザイクウイルスに感染したハクサイにおける病徴決定因子の遺伝学的解析 (2008)
  • Author(s): 藤原綾香
  • Organizer: 平成20年度日本植物病理学会大会北海道部会
  • Place of Presentation: 札幌市、北海道農業研究センター
  • Year and Date: 2008-10-17
• [Presentation] マクロアレイとマイクロチューブハイブリダイゼーションを用いた重複感染ウイルスの簡易高感度検出法 (2008)
  • Author(s): 杉山俊平
  • Organizer: 平成20年度日本植物病理学会大会北海道部会
  • Place of Presentation: 札幌市、北海道農業研究センター
  • Year and Date: 2008-10-17
• [Presentation] 植物RNAウイルスを用いて誘導される外来性遺伝子の転写不活性化におけるベクター挿入配列の段階的な長さの違いによる効果 (2008)
  • Author(s): 河合文珠
  • Organizer: 日本育種学会第114回講演会
  • Place of Presentation: 彦根市、滋賀県立大学
  • Year and Date: 2008-10-11
• [Presentation] DNA脱メチル化酵素遺伝子の発現量は植物ウイルスベクターを介した外来性プロモーターへのメチル化誘導の程度に影響する (2008)
  • Author(s): 太田垣駿吾
  • Organizer: 日本育種学会第114回講演会
  • Place of Presentation: 彦根市、滋賀県立大学
  • Year and Date: 2008-10-11
• [Presentation] フラボノイド合成系遺伝子のRNAサイレンシングにより明らかになった植物組織の着色に必要なm RNA量の閾値の存在 (2008)
  • Author(s): 永松敦
  • Organizer: 日本遺伝学会第80回大会
  • Place of Presentation: 名古屋市、名古屋大学
  • Year and Date: 2008-09-05
• [Presentation] methylation induced by a plant virus vector on the CaMV 35S promoter and transcriptional repression (2008)
  • Author(s): Otagaki, S
  • Organizer: Biological Interactions-with Plant Roots and Aerial Tissues
  • Place of Presentation: Hokkaido Univ., Sapporo
  • Year and Date: 2008-07-28
• [Presentation] クローバ葉脈黄化ウイルスの病原性に関わる宿主タンパク質の同定 (2008)
  • Author(s): 山田翔太、中原健二、上田一郎, 他
  • Organizer: 日本ウイルス学会北海道支部 第42回夏期シンポジウム
  • Place of Presentation: ニセコ町、ホテルニセコいこいの村
• [Presentation] クローバ葉脈黄化ウイルスの病原性に関わる宿主タンパク質の同定 (2008)
  • Author(s): 山田翔太
  • Organizer: 日本ウイルス学会北海道支部第42回夏期シンポジウム
  • Place of Presentation: ニセコ町、ホテルニセコいこいの村
• [Presentation] eukaryotic initiation factor 4E involved in a recessive resistance to Clover yellow vein virus in pea carrying cyv2 (2008)
  • Author(s): Nakahara, K.
  • Organizer: International Congress of Virology, The INTERN ATIONAL UNION OF MICROBIOLOGICAL SOCIETIES
  • Place of Presentation: Istanbul, ISTANBUL CON VENTION AND EXHIBITION CENTRE
• [Presentation] Comparison of the 2b proteins from two isolates of Tomato aspermy virus in silencing suppressor activity and viral systemic movement (2008)
  • Author(s): Shimura, H.
  • Organizer: International Congress of Virology, The INTERNATIONAL UNION OF MICROBIOLOGICAL SOCIETIES
  • Place of Presentation: Istanbul, ISTANBUL CONVENTION AND EXHIBITION CENTRE
• [Presentation] Induction of trascriptional gene silencing of an endogenous gene in petunic by the Cucumber mosaic virus vector (2008)
  • Author(s): Inaba, J.
  • Organizer: International Congress of Virology, The INTERNATIONAL UNION OF MICROBIOLOGICAL SOCIETIES
  • Place of Presentation: Istanbul, ISTANBUL CONVENTION AND EXHIBITION CENTRE
• [Presentation] 植物におけるウイルスベクターを介した外来性プロモーターのメチル化の誘導效率の違いが転写活性に及ぼす影響 (2007)
  • Author(s): 太田垣 駿吾
  • Organizer: 日本分子生物学会第30回年会
  • Place of Presentation: 横浜、パシフィコ横浜
  • Year and Date: 2007-12-13
• [Presentation] Virus-induced gene silencingを利用したダイズにおけるフラボノイドの生合成に関わる遺伝子の機能解析と組成の改変 (2007)
  • Author(s): 永松 敦
  • Organizer: 日本分子生物学会第30回年会
  • Place of Presentation: 横浜、パシフィコ横浜
  • Year and Date: 2007-12-13
• [Presentation] ダイズにおいてF3' HmRNA蓄積量が毛茸の着色に関与する (2007)
  • Author(s): 永松 敦
  • Organizer: 札幌農林学会平成19年度大会
  • Place of Presentation: 札幌、北海道大学
  • Year and Date: 2007-12-02
• [Presentation] エンドウにおけるクローバー葉脈黄化ウイルスの病原性にはRNA silencing suppressor活性が関与する (2007)
  • Author(s): 厚見剛、中原健二、上田一郎, 他
  • Organizer: 第55回日本ウイルス学会
  • Place of Presentation: 札幌、札幌コンベンションセンター
  • Year and Date: 2007-10-22
• [Presentation] キュウリモザイクウイルスベクターを用いたペチュニアの内在性遺伝子プロモーターへのメチル化誘導 (2007)
  • Author(s): 稲場純一、増田税, 他
  • Organizer: 平成19年度日本植物病理学会北海道部会
  • Place of Presentation: 札幌、北方圏センター
  • Year and Date: 2007-10-12
• [Presentation] キュウリモザイクウイルスされライトRNAは宿主RNAサイレンシングを促進する (2007)
  • Author(s): 小暮勇樹、増田税, 他
  • Organizer: 平成19年度日本植物病理学会北海道部会
  • Place of Presentation: 札幌、北方圏センター
  • Year and Date: 2007-10-12
• [Presentation] シロクローバモザイクウイルスRC株のベクター化のための感染性cDNAの構築とサブゲノムRNAの解析 (2007)
  • Author(s): 井門 由佳里
  • Organizer: 平成19年度日本植物病理学会北海道部会
  • Place of Presentation: 札幌、北方圏センター
  • Year and Date: 2007-10-12
• [Presentation] キュウリモザイクウイルスのタバコでの全身移行に及ぼす2bタンパク質の影響 (2007)
  • Author(s): 辺見 一広
  • Organizer: 平成19年度日本植物病理学会北海道部会
  • Place of Presentation: 札幌、北方圏センター
  • Year and Date: 2007-10-12
• [Presentation] Virus-induced gene silencingを利用したダイズフラボノイド生合成の改変 (2007)
  • Author(s): 永松 敦
  • Organizer: 日本育種学会第112回講演会
  • Place of Presentation: 鶴岡、山形大学
  • Year and Date: 2007-09-22
• [Presentation] クローバー葉脈黄化ウイルスの病原性におけるサリチル酸経路の役割について (2007)
  • Author(s): 上田 一郎
  • Organizer: 植物感染生理談話会
  • Place of Presentation: 京都、京都府立大学
  • Year and Date: 2007-08-10
• [Presentation] Clover yellow vein virus expresses virulence by salicylic acid-mediated pathway in pea. (2007)
  • Author(s): Atsumi G., Nakahara S., Uyeda I. 他
  • Organizer: XIII International Congress on Molecular Plant-Microbe Interactions
  • Place of Presentation: イタリア、ソレント、ホテルヒルトン
  • Year and Date: 2007-07-25
• [Presentation] Clover yellow vein virus expresses virulence by salicylic acid-mediated pathway in pea (2007)
  • Author(s): G. Atsumi
  • Organizer: XIII International Congress on Molecular Plant-Mierobe Interactions
  • Place of Presentation: イタリア、ソレント、ホテルヒルトン
  • Year and Date: 2007-07-25
• [Presentation] RNAサイレンシング抑制遺伝子による外来遺伝子の発現促進について (2007)
  • Author(s): 中原健二、上田一郎, 他
  • Organizer: 第41回日本ウイルス学会北海道支部夏期シンポジウム
  • Place of Presentation: 小樽、かんぽの宿朝里荘
• [Presentation] プロトプラストを使用したウイルスのサイレンシングサプレッサーの機能解析 (2007)
  • Author(s): 志村華子、増田税, 他
  • Organizer: 平成19年度日本植物病理学会大会
  • Place of Presentation: 宇都宮、宇都宮大学農学部
• [Presentation] エンドウにおいてクローバー葉脈黄化ウイルスが誘導する全身性の細胞死とRNA silencing suppressor活性の関係 (2007)
  • Author(s): 厚見剛、中原健二、上田一郎, 他
  • Organizer: 平成19年度日本植物病理学会大会
  • Place of Presentation: 宇都宮、宇都宮大学農学部
• [Book] In Antiviral Resistance in Plants (Methods in Molecular Biology)(M.B., Wang, J., Watson, Humana Press, Totowa, NJ, USA,) (2009)
  • Author(s): Shimura, H. and Masuta, C.
  • Publisher: Functional analysis of CMV satellite RNAs in RNA silencing.(in press)
• [Book] 北海道大学出版会 (2007)
  • Author(s): 上田 一郎
  • Total Pages: 231
  • Publisher: 微生物の病原性と植物の防御応答
• [Remarks] ホームページ
  • URL: http://www.agr.hokudai.ac.jp/ikushu/byougen/saito/purojekuto_yan_jiu.html
• [Remarks]
  • URL: http://www.agr.hokudai.ac.jp/gs/education/res.html#22
• [Remarks]
  • URL: http://www.agr.hokudai.ac.jp/gs/education/res.html#22
• [Patent(Industrial Property Rights)] 植物ウイルスベクター (2007)
  • Inventor(s): 増田税, 他
  • Industrial Property Rights Holder: 北海道大学
  • Acquisition Date: 2007-09-14
• [Patent(Industrial Property Rights)] 外来遺伝子を持たない形質転換植物及びその生産方法 (2006)
  • Inventor(s): 金澤章・増田税・中原健二
  • Industrial Property Number: 2006-254801
  • Filing Date: 2006-09-20