Development of regenerative medicine by the human laminins mass-produced by yeast

• Project/Area Number: 18108003
• Research Category: Grant-in-Aid for Scientific Research (S)
• Allocation Type: Single-year Grants
• Research Field: Applied biochemistry
• Research Institution: Nagoya University
• Principal Investigator: NIIMI Tomoaki Nagoya University, 大学院・生命農学研究科, 助教 (30377791)
• Co-Investigator(Kenkyū-buntansha): KITAGAWA Yasuo 名古屋大学, 大学院・生命農学研究科, 教授 (50101168) ; TORII Shuhei 名古屋大学, 大学院・医学系研究科, 教授 (60115607) ; KADOWAKI Tatsuhiko 名古屋大学, 大学院・生命農学研究科, 准教授 (90313973)
• Project Period (FY): 2006 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥79,820,000 (Direct Cost: ¥61,400,000、Indirect Cost: ¥18,420,000); Fiscal Year 2009: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000); Fiscal Year 2008: ¥25,090,000 (Direct Cost: ¥19,300,000、Indirect Cost: ¥5,790,000); Fiscal Year 2007: ¥22,880,000 (Direct Cost: ¥17,600,000、Indirect Cost: ¥5,280,000); Fiscal Year 2006: ¥25,350,000 (Direct Cost: ¥19,500,000、Indirect Cost: ¥5,850,000)
• Keywords: 細胞培養 / ラミニン / 脂肪細胞 / 再生医療

Research Abstract

We proposed a model in which the cryptic α4 LG4-5 fragment derived from laminin-411 inhibits de novo adipogenesis by modulating the effect of FGF-2 through syndecans. The establishment of a mass-production system for recombinant laminins using non-pathogenic Leishmania tarentolae will be useful for cell biology and tissue engineering applications.

Research Products

• [Journal Article] Hepatic stellate cells stimulate HCC cell migration vialaminin-5 production. (2011)
  • Author(s): Santamato, A., Fransvea, E., Dituri, F., Caligiuri, A., Quaranta, M., Niimi, T., Pinzani, M., Antonaci, S., Giannelli, G.
  • Journal Title: Clin.Sci. 121 Pages: 159-168
  • Peer Reviewed
• [Journal Article] The production of recombinant human laminin-332 in a Leishmania tarentolae expression system. (2009)
  • Author(s): Phan, H.-P., Sugino, M., Niimi, T.
  • Journal Title: Protein Expr Purif 68 Pages: 79-84
  • Peer Reviewed
• [Journal Article] Novel Culture System of Mesenchymal Stromal Cells from Human Subcutaneous Adipose Tissue. (2009)
  • Author(s): Iwashima, S., Ozaki, T., Maruyama, S., Saka, Y., Kobori, M., Omae, K., Yamaguchi, H., Niimi, T., Toriyama K., Kamei Y., Torii S., Murohara, T., Yuzawa, Y., Kitagawa Y., Matsuo, S.
  • Journal Title: Stem Cells Dev. 18 Pages: 533-543
  • Peer Reviewed
• [Journal Article] Expression and chain assembly of human laminin-332 in an insect cell-free translation system. (2008)
  • Author(s): Phan, H.-P., Ezure, T., Ito, M., Kadowaki, T., Kitasawa, Y., Niimi, T.
  • Journal Title: Biosci.Biotechnol.Biochem. 72 Pages: 1847-1852
  • NAID: 10027530048
  • Peer Reviewed
• [Journal Article] Cryptic fragment α4 LG4-5 derived from laminin α4 chain inhibits de novo adipogenesis by modulating the effect of fibroblast growth factor-2. (2008)
  • Author(s): Yamashita, H., Goto, C., Tajima, R., Koparal, A.T., Kobori M., Ohki, Y, Shitara, K., Narita, R., Toriyama K., Torii S., Niimi, T., Kitagawa, Y.
  • Journal Title: Dev.Growth Diff. 50 Pages: 97-107
  • NAID: 10021095600
  • Peer Reviewed
• [Journal Article] Recombinant sendai virus-mediated gene transfer to adipose tissue-derived stein cells (ASCs). (2008)
  • Author(s): Yukawa, H., Noguchi, H., Oishi, K., Miyazaki T., Kitasawa, Y., Inoue, M., Hasegawa, M., Hayashi S.
  • Journal Title: Cell Transplant. 17 Pages: 43-50
  • Peer Reviewed
• [Journal Article] Cryopreservation of mouse adipose tissue-derived stem/progenitor cells. (2008)
  • Author(s): Oishi, K., Noguchi, H., Yukawa, H., Miyazaki T., Kato, R., Kitasawa, Y., Ueda, M., Hayashi, S.
  • Journal Title: Cell Transplant. 17 Pages: 35-41
  • Peer Reviewed
• [Journal Article] Cryptic fragment α4 LG4-5 derived from laminin α4 chain inhibits de novo a dipogenesis by modulating the effect of fibroblast growth factor-2 (2008)
  • Author(s): Yamashita, H., et al
  • Journal Title: Development, Growth & Differentiation 50 Pages: 97-1007
  • Peer Reviewed
• [Journal Article] The Sp family of transcription factors regulates the human laminin α1 gene in JAR choriocarcinoma cells. (2006)
  • Author(s): Niimi, T., Hayashi, Y., Sekiguchi, K., Kitasawa, Y.
  • Journal Title: Biochim.Biophys.Acta. 1759 Pages: 573-579
  • Peer Reviewed
• [Journal Article] 脂肪組織由来幹細胞のマクロファージ様挙動 (2006)
  • Author(s): 宮崎隆道, 北川泰雄
  • Journal Title: 再生医療 5 Pages: 497-501
  • Peer Reviewed
• [Journal Article] 脂肪組織由来幹細胞発見の経緯と臨床応用にっいて (2006)
  • Author(s): 北川泰雄, 小堀雅登, 鳥山和宏, 亀井譲, 鳥居修平
  • Journal Title: 形成外科 49 Pages: 1087-1104
• [Journal Article] The Sp family of transcription factors regulates the human laminin alpha 1 gene in JAR choriocarcinoma cells (2006)
  • Author(s): Niimi, T. et al.
  • Journal Title: Biochimica et Biophysica Acta 1759 Pages: 573-579
• [Journal Article] ヒト脂肪由来幹細胞発見の経緯と臨床応用について (2006)
  • Author(s): 北川泰雄他
  • Journal Title: 形成外科 49 Pages: 1087-1104
• [Presentation] Development of mass production system on the basement membrane protein laminin. (2010)
  • Author(s): 新美友章
  • Organizer: International symposium 2010 for Healthcare
  • Place of Presentation: Woosuk University, Jeoniu, Korea
• [Presentation] 新美友章骨粗鬆症モデルマウスにおける脂肪由来幹細胞を用いた再生治療の検討 (2009)
  • Author(s): 山崎浩志, 坂洋祐, 丸山彰一
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフイコ横浜
• [Presentation] The production and purification of recombinant human Laminin-332 in Lelshmania tarentolae expression system (2009)
  • Author(s): Sugino M., Phan, H.-P., Niimi T.
  • Organizer: 第8回環太平洋結合組織学会シンポジウム・第41回日本結合組織学会学術大会・第56回マトリックス研究会大会合同学術集会
  • Place of Presentation: 湘南国際村センター
• [Presentation] Leishmania tarentolae発現系を用いた基底膜蛋白質の生産 (2009)
  • Author(s): 杉野麻梨沙, Phan Phuong Hoang, 新美友章
  • Organizer: 日本農芸化学会2009年度大会
  • Place of Presentation: 福岡国際会議場
• [Presentation] 無細胞蛋白質合成系を用いたラミニン鎖会合におけるジスルフィド結合様式の解析 (2008)
  • Author(s): Phan Phuong Hoang, 江連徹, 伊東昌章, 門脇辰彦, 新美友章
  • Organizer: BMB2008(第31回日本分子生物学会年会・第81回日本生化学会大会合同大会)
  • Place of Presentation: 神戸国際会議場
• [Presentation] 無細胞蛋白質合成系を用いたラミニンサブユニット会合機構の解析 (2008)
  • Author(s): Phan Phuong Hoang, 江連徹, 伊東昌章、門脇辰彦, 北川泰雄, 新美友章
  • Organizer: 第40回日本結合組織学会学術大会・第55回マトリックス研究会大会合同学術集会
  • Place of Presentation: 駒場エミナース
• [Presentation] 骨粗鬆症モデルマウスへの脂肪組織由来幹細胞の尾静脈注入による骨密度上昇 (2007)
  • Author(s): 今泉正恭, 北川泰雄
  • Organizer: 日本農芸化学会2007年度大会
  • Place of Presentation: 東京大学
• [Presentation] 脂肪組織由来自家幹細胞の臨床応用の可能性 (2007)
  • Author(s): 北川泰雄
  • Organizer: 第19回代用臓器・再生医学研究会
  • Place of Presentation: 北海道大学
• [Presentation] Selective expansion of mesenchymal stem cells by culturing stromal vascular fraction obtained from human omentum in low serum medium. (2006)
  • Author(s): Toriyama K., Omae, K, Yamaguchi, H., Imaizumi M., Yamauchi, Y., Kamei, Y., Torii S., Kitagawa Y.
  • Organizer: The 4^<th> Meeting of International Fat Applied Technology Society
  • Place of Presentation: Baton Rouge USA
• [Presentation] Macrophage-like behavior of adipose-derived stem cells in co-culture with adipocytes and their debris. (2006)
  • Author(s): Miyazaki, T., Toriyama, K., Torii, S., Kitagawa, Y.
  • Organizer: The 4^<th> Meeting of International Fat Applied Technology Society
  • Place of Presentation: Baton Rouge USA
• [Presentation] Transient increase of bone mineral density by tail vein injection of adipose-derived mesenchymal stem cells into OPG/OCIF-deficient mice as a model of osteoporosis. (2006)
  • Author(s): Imaizumi M., Takamatsu, S., Fuseya, A., Miyazaki, T., Kitagawa Y.
  • Organizer: The 4^<th> Meeting of International Fat Applied Technology Society
  • Place of Presentation: Baton Rouge USA
• [Presentation] De nova adipogenesis at the site of injection of alginate in combination with fibroblast growth factor-2 and heparin. (2006)
  • Author(s): Miyazaki, T., Yamauchi, Y., Toriyama K., Torii S., Kitagawa Y.
  • Organizer: The 4^<th> Meeting of International Fat Applied Technology Society
  • Place of Presentation: Baton Rouge USA
• [Presentation] ラミニンα4鎖の断片はラミニン-8から切断され線維芽細胞増殖の作用を制御する (2006)
  • Author(s): 竹ノ内和貴, 後藤千絵, 山下泰恒, 田島理恵, Ayse Tanse Koparal, 小堀雅登, 大木裕司, 設楽研也, 鳥山和宏, 鳥居修平, 北川泰雄
  • Organizer: 日本農芸化学会中部支部第147回例会
  • Place of Presentation: 名古屋大学
• [Presentation] Adipose-derived stem cells with muhiple potential of differentiation (2006)
  • Author(s): 北川泰雄
  • Organizer: 第11回アディポサイエンス研究会シンポジウム
  • Place of Presentation: 千里阪急ホテル
• [Book] Leishmania tarentolae : A review.Methods in Molecular Biology.((Lorence, A.ed.)(Recombinant protein production in the eukaryotic protozoan parasite)
  • Author(s): Niimi, T.
  • Publisher: Humana Press, NJ, USA(in press)
• [Book] Leishmania tarentolae. Methods in Molecular Biology.(Lorence, A.ed.)(Expression of multi-subunit proteins in)
  • Author(s): Sugino, M., Niimi, T.
  • Publisher: Humana Press, NJ, USA(in press)
• [Remarks] ホームページ等
• [Patent(Industrial Property Rights)] ラミニンコイルドコイル(LCC)ドメインヘテロ多量体を含むタンパク質会合体の調製方法 (2008)
  • Inventor(s): 新美友章、門脇辰彦、ファン・ホアング・フォング、伊東昌章、江連徹
  • Industrial Property Rights Holder: 島津製作所、名古屋大学
  • Industrial Property Number: 2008-055649
  • Filing Date: 2008-03-06
• [Patent(Industrial Property Rights)] ラミニンコイルドコイル(LCC)ドメインヘテロ多量体を含むタンパク質会合体の調製方法 (2008)
  • Inventor(s): 新美友章, 門脇辰彦, ファン・ホアング・フォンダ, 伊東昌章, 江連徹
  • Industrial Property Rights Holder: 新美友章, 門脇辰彦, ファン・ホアング・フォンダ, 伊東昌章, 江連徹
  • Industrial Property Number: 2008-055649
  • Filing Date: 2008-03-06