| Budget Amount *help |
¥111,800,000 (Direct Cost: ¥86,000,000、Indirect Cost: ¥25,800,000)
Fiscal Year 2010: ¥22,100,000 (Direct Cost: ¥17,000,000、Indirect Cost: ¥5,100,000)
Fiscal Year 2009: ¥22,100,000 (Direct Cost: ¥17,000,000、Indirect Cost: ¥5,100,000)
Fiscal Year 2008: ¥22,100,000 (Direct Cost: ¥17,000,000、Indirect Cost: ¥5,100,000)
Fiscal Year 2007: ¥22,100,000 (Direct Cost: ¥17,000,000、Indirect Cost: ¥5,100,000)
Fiscal Year 2006: ¥23,400,000 (Direct Cost: ¥18,000,000、Indirect Cost: ¥5,400,000)
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| Research Abstract |
The goal of this study is to elucidate molecular mechanisms by which pregnancy is established in mammalian species. Among several bovine interferon tau (IFNT) genes, only two of them were expressed in vivo, one of IFNTs induced Mx2expression by endometrial cells. Addition of uterine flushing media mimicked the expression of IFNT and CDX2/EOMES transcription factors in the endometrial and trophoblast CT-1co-culture system. In the bovine uterus, attachment of elongated conceptus to the maternal epithelium induced changes in epigenetic status of IFNT gene: reduction in H3 acetylation and increase in H3 methylation. In addition, the conceptus expresses mesenchymal genes while keeping epithelial cell nature. Furthermore, several novel genes were identified during the pre-attachment period, which could be used to detect early pregnancy in the bovine species. Through these experimentations, novel mechanisms regulating pregnancy establishment could be established.
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