Regulation mechanism of information transmission at single central synapses

• Project/Area Number: 18200023
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neuroscience in general
• Research Institution: Kyoto University
• Principal Investigator: HIRANO Tomoo Kyoto University, 大学院・理学研究科, 教授 (50181178)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥38,870,000 (Direct Cost: ¥29,900,000、Indirect Cost: ¥8,970,000); Fiscal Year 2008: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000); Fiscal Year 2007: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000); Fiscal Year 2006: ¥21,450,000 (Direct Cost: ¥16,500,000、Indirect Cost: ¥4,950,000)
• Keywords: シナプス / 海馬 / 小脳 / 錐体細胞 / プルキンエ細胞 / スパイン / グルタミン酸 / 顆粒細胞 / 培養 / グルタミン酸受容体 / 樹状突起 / 多様性 / 長期増強

Research Abstract

哺乳類中枢神経系内シナプスの機能と形態の性質およびそれらの相関を単一シナプスレベルで解明することをめざし、海馬錐体細胞と小脳プルキンエ細胞上に形成されるグルタミン酸作動性興奮性ミナプスを比較研究した。海馬のシナプスにおいては、シナプス応答の強さとシナプス後部スパインの大きさが相関し、小脳シナプスにおいてはシナプス前膨大とシナプス後スパインの大きさが相関するなど、両シナプスの違いが明らかになった。

Research Products

• [Journal Article] Prediction and validation of a mechanism to control the threshold for inhibitory synaptic plasticity (2009)
  • Author(s): Kitagawa, Y., Hirano, T., Kawaguchi, S.
  • Journal Title: Mol.Syst.Biol. 5, 280 Pages: 1-16
  • Peer Reviewed
• [Journal Article] Postsynaptic glutamate receptor δ family contributes to presynaptic terminal differentiation and establishment of synaptic transmission (2009)
  • Author(s): Kuroyanagi, T., Yokoyama, M., Hirano. T.
  • Journal Title: Proc.Natl.Acad.Sci.USA 106 Pages: 4912-4916
  • Peer Reviewed
• [Journal Article] mGluRl-mediated facilitation of long-term potentiation at inhibitory synapses on a cerebellar Purkinje neuron (2008)
  • Author(s): Y., Kawaguchi, S., Hirano, T.
  • Journal Title: Eur.J.Neurosci. 27 Pages: 884-896
  • Peer Reviewed
• [Journal Article] Src-family protein tyrosine kinase negatively regulates cerebellar long-term depression. (2008)
  • Author(s): Tsuruno S, Kawaguchi S, Hirano T
  • Journal Title: Neurosci. Res. 61 Pages: 329-332
  • Peer Reviewed
• [Journal Article] Enhancement of both log-term depression induction and optokinetic response adaptation in mice lacking delphilin. (2008)
  • Author(s): Takeuchi T, et al.
  • Journal Title: PLoS ONE 3
  • Peer Reviewed
• [Journal Article] Bidirectional plasticity at developing climbing fiber - Purkinje neuron synapses. (2008)
  • Author(s): Ohtsuki G, Hirano T
  • Journal Title: Eur. J. Neurosci. 28 Pages: 2393-2400
  • Peer Reviewed
• [Journal Article] mGluR1-mediated facilitation of long-term potentiation at inhibitory synapses on a cerebellar Purkinje neuron. (2008)
  • Author(s): Sugiyama, Y., Kawaguchi, S. & Hirano, T.
  • Journal Title: European Journal of Neuroscience 27 Pages: 884-896
  • Peer Reviewed
• [Journal Article] Sustained GABARAP structural change underlies long-term potentiation at inhibitory synapses on a cerebellar Purkinje neuron (2007)
  • Author(s): Kawaguchi, S., Hirano T.
  • Journal Title: J.Neurosci 27 Pages: 6788-6799
  • Peer Reviewed
• [Journal Article] Persistent activation of protein kinase Cα is not necessary for expression of cerebellar long-term depression (2007)
  • Author(s): Tsuruno, S., Hirano, T.
  • Journal Title: Mol.Cell Neurosci 35 Pages: 38-48
  • Peer Reviewed
• [Journal Article] Sustained GABARAP structural change underlies long-term potentiation at inhibitory synapses on a cerebellar Purkinje neuron. (2007)
  • Author(s): Kawaguchi, S. & Hirano, T.
  • Journal Title: Journal of Neuroscience 27 Pages: 6788-6799
  • Peer Reviewed
• [Journal Article] Persistent activation of protein kinase Cα is not nessesary for expression of cerebellar long-term depression. (2007)
  • Author(s): Tsuruno, S., Hirano, T.
  • Journal Title: Molecular and Cellular Neuroscience (印刷中)
• [Journal Article] Targeted disruption of Sept3, a binding partner of Sept5 and Sept7, unaffects neuronal development in the central nervous system. (2007)
  • Author(s): Fujishima, K., Kurisu, J., Hirano, T., Kengaku, M.
  • Journal Title: Journal of Neurochemistry (印刷中)
• [Journal Article] Increased occurrence of climbing fiber inputs to the cerebellar flocculus in a mutant mouse is correlated with the timing delay of optokinetic response. (2007)
  • Author(s): Yoshida, T., Funabiki, K., Hirano, T.
  • Journal Title: European Journal of Neuroscience (印刷中)
• [Journal Article] Membrane proximal region of GluRδ2 is critical for LTD and interaction with PICK1 in a cerebellar Purkinje neuron. (2006)
  • Author(s): Yawata, S., Tsuchida, H., Kengaku, M., Hirano, T.
  • Journal Title: Journal of Neuroscience 26 Pages: 3626-3633
• [Presentation] Correlations between function and morphology of single synapses on hippocampal pyramidal neurons and cerebellar Purkinje neurons are different (2009)
  • Author(s): Miyawaki, H., Hirano, T.
  • Organizer: 39th Annual Meeting of the Society for Neuroscience, Chicago
  • Place of Presentation: McCormick Place Convention Center
  • Year and Date: 2009-10-21
• [Presentation] Correlations between function and morphology of single synapses on hippocampal pyramidal neurons and cerebellar Purkinje neurons are different (2009)
  • Author(s): Miyawaki, H., Hirano, T.
  • Organizer: 32nd annual meeting of the Japan Neuroscience Society, Nagoya
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2009-09-17
• [Presentation] Roles of glutamate receptor δ family in synapse formation (2009)
  • Author(s): Hirano, T.
  • Organizer: Symposium "Novel aspects of the function of ionotropic & metabotropic glutamate receptors" in the 32nd annual meeting of the Japan Neuroscience Society, Nagoya
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2009-09-16
• [Presentation] 小脳プルキンエ細胞における個々のシナプスでのEPSCの特性 (2008)
  • Author(s): 宮脇寛行, 平野丈夫
  • Organizer: 第31回日本神経科学大会
  • Place of Presentation: 東京、東京国際フォーラム
  • Year and Date: 2008-07-11
• [Presentation] 小脳プルキンエ細胞における個々のシナプスでのEPSCの特性 (2008)
  • Author(s): 宮脇寛行, 平野丈夫
  • Organizer: 第31回日本神経科学大会
  • Place of Presentation: 東京
  • Year and Date: 2008-07-11
• [Presentation] Regulation of synaptic transmission and motor learning (2008)
  • Author(s): Hirano. T.
  • Organizer: International symposium on hierarchy and holism : Bridging across different hierarchies in natural sciences. Okazaki
  • Place of Presentation: 自然科学研究機構
  • Year and Date: 2008-02-22
• [Presentation] Critical role of GABARAP in long-term potentiation at inhibitory synapses on a Purkinje neuron (2007)
  • Author(s): Kawaguchi, S., Hirano, T.
  • Organizer: 37th Annual Meeting of the Society for Neuroscience, San Diego
  • Place of Presentation: San Diego Convention Center
  • Year and Date: 2007-11-06
• [Presentation] Critical role of GABARAP in long-term potentiation at inhibitory synapses on a Purkinje neuron. (2007)
  • Author(s): Kawaguchi, S. & Hirano, T.
  • Organizer: 第37回北米神経科学会
  • Place of Presentation: San Diego, CA, USA
  • Year and Date: 2007-11-06
• [Presentation] Molecular mechanism of inhibitory synaptic plasticity in the cerebellum. (2007)
  • Author(s): Kawaguchi, S. & Hirano, T.
  • Organizer: 第30回日本神経科学大会
  • Place of Presentation: 横浜
  • Year and Date: 2007-09-11
• [Presentation] グルタミン酸受容体δ2サブユニットは小脳顆粒細胞のシナプス前終末の分化を誘導する (2007)
  • Author(s): 畔柳智明、平野丈夫
  • Organizer: 第30回日本神経科学大会
  • Place of Presentation: 横浜
  • Year and Date: 2007-09-11
• [Presentation] Regulatory mechanism of inhibitory synaptic transmission in the cerebellum (2006)
  • Author(s): Kawaguchi, S., Hirano. T.
  • Organizer: Symposium "Regulatory mechanism of GABAergic synaptic transmission" in the 29th annual meeting of the Japan Neuroscience Society, Kyoto
  • Place of Presentation: 京都国際会館
  • Year and Date: 2006-07-21
• [Remarks]
  • URL: http://neurosci.biophys.kyoto-u.ac.jp/
• [Remarks]
  • URL: http://neurosci.biophys.kyoto-u.ac.jp/