Development of a novel targeted single cell drug/gene delivery system

• Project/Area Number: 18200036
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Medical systems
• Research Institution: Fukuoka University
• Principal Investigator: TACHIBANA Katsuro Fukuoka University, 医学部, 教授 (40271605)
• Co-Investigator(Kenkyū-buntansha): AI Hiroyuki 福岡大学, 理学部, 助教 (20330897)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥41,730,000 (Direct Cost: ¥32,100,000、Indirect Cost: ¥9,630,000); Fiscal Year 2008: ¥9,490,000 (Direct Cost: ¥7,300,000、Indirect Cost: ¥2,190,000); Fiscal Year 2007: ¥12,350,000 (Direct Cost: ¥9,500,000、Indirect Cost: ¥2,850,000); Fiscal Year 2006: ¥19,890,000 (Direct Cost: ¥15,300,000、Indirect Cost: ¥4,590,000)
• Keywords: 超音波エネルギー / 神経細胞 / 周波数 / 超小型超音波プローブ / 遺伝子導入

Research Abstract

本課題の目標であった超音波照射時における浮遊単細胞のリアルタイム顕微鏡観察システムを構築することができた。また、高速ビデオカメラにてマイクロバブルの挙動を観察すると同時に薬物が細胞内に流入する現象を初めてデジタル映像でとらえることができた。この顕微鏡システムを用いることで超音波の最適条件と各種のマイクロバブルの比較が可能となった。得られたデータ解析より、多種の細胞にこの技術が応用可能と考えられる。

Research Products

• [Journal Article] Evaluation and comparison of three novel microbubbles : enhancement of ultrasound-induced cell death and free radicals production (2009)
  • Author(s): Hassan MA, Feril LB Jr, Suzuki K, Kudo N, Tachibana K, Kondo T
  • Journal Title: Ultrason Sonochem 16(3) Pages: 372-8
  • Peer Reviewed
• [Journal Article] Response characteristics of vibration-sensitive interneurons related to Johnston's organ in the honeybee, Apis nielllfera (2009)
  • Author(s): Hiroyuki Ai, Jurgen Rybak, Randolf Menzel, Tsunao Itoh
  • Journal Title: J. Comp. Neurol. 515 Pages: 145-160
  • Peer Reviewed
• [Journal Article] A novel strategy utilizing ultrasound for antigen delivery in dendritic cell-based cancer immunotherapy (2009)
  • Author(s): Suzuki R
  • Journal Title: J Control Release. 133 Pages: 198-205
  • Peer Reviewed
• [Journal Article] Evaluation and comparison of three novel microbubbles : Enhancement of ultrasound-induced cell death and free radicals production (2009)
  • Author(s): Hassan MA
  • Journal Title: Ultrason Sonochem. 16/3 Pages: 372-378
  • Peer Reviewed
• [Journal Article] Gene Transfection through microbubble-aided sonication in cancer cells (2009)
  • Author(s): Dickerson C. Moreno
  • Journal Title: Philippine Physics Journal 31 Pages: 1-6
  • Peer Reviewed
• [Journal Article] 超音波感受性マイクロバブルを利用した血管壁へのドラッグデリバリー (2008)
  • Author(s): 立花克郎
  • Journal Title: 血管医学 9(3) Pages: 287-291
  • Peer Reviewed
• [Journal Article] Sonodynamic therapy (2008)
  • Author(s): Tachibana K, Feril LB Jr, Ikeda-Dantsuji Y
  • Journal Title: Ultrasonics 48(4) Pages: 253-259
  • Peer Reviewed
• [Journal Article] Therapeutic potential of low-intensity ultrasound (part 2) : biomolecular effects, sonotransfection, and sonoporation (2008)
  • Author(s): Feril LB Jr, Tachibana K, Ikeda-Dantsuji Y, Endo H, Harada Y, Kondo T, Ogawa R
  • Journal Title: Journal of Medical Ultrasonics 35 Pages: 161-167
  • Peer Reviewed
• [Journal Article] Three-dimensional reconstruction of neural tracts and commissures in the bee brain (2008)
  • Author(s): H Ai, Y Kusakabe, T Itoh
  • Journal Title: Comparative Biochemistry and Physiology, Part B, Biochemistry & molecular biology 151(4) Pages: 457-457
  • Peer Reviewed
• [Journal Article] Therapeutic Potential of Low-Intensity Ultrasound(Part1) : Thermal and Sonomechanical Effects (2008)
  • Author(s): Feril Loreto, Tachibana K, Ogawa K, Irie Y
  • Journal Title: J. Med Ultrasonics 35 Pages: 153-160
  • NAID: 10025327393
  • Peer Reviewed
• [Journal Article] Therapeutic Potential of Low-Intensity Ultrasound(Part1) : Thermal and Sonomechanical Effects (2008)
  • Author(s): FERIL Loreto
  • Journal Title: J. Med Ultrasonics 35 Pages: 153-160
  • Peer Reviewed
• [Journal Article] Therapeutic potentials of low-intensity ultrasound (part II) : Biomolecular effects, sonotransfection and sonopermeabilization (2008)
  • Author(s): FERIL Loreto
  • Journal Title: J. Med Ultrasonics 35 Pages: 161-167
  • Peer Reviewed
• [Journal Article] Genetic networks responsive to low-intensity pulsed ultrasound in human lymphoma U937 cells (2008)
  • Author(s): Tabuchi Y
  • Journal Title: Cancer Letters 270/2 Pages: 286-294
  • Peer Reviewed
• [Journal Article] Sonodynamic Therapy (2008)
  • Author(s): Tachibana Katsuro
  • Journal Title: Ultrasonic 48 Pages: 253-259
  • Peer Reviewed
• [Journal Article] Growth inhibition of neurofibroma by ultrasound-mediated interferon γ transfection (2008)
  • Author(s): Yamaguchi K
  • Journal Title: Journal of Medical Ultrasonics 36 Pages: 3-8
  • Peer Reviewed
• [Journal Article] 超音波を利用した部位特異的遺伝子デリバリーシステムの開発 (2008)
  • Author(s): 鈴木 亮、小田 雄介、生井 栄祐、滝澤 知子、根岸 洋一、宇都宮 直樹、立花 克郎、丸山 一雄
  • Journal Title: 薬学雑誌 128(2) Pages: 187-1192
  • NAID: 110006572172
  • Peer Reviewed
• [Journal Article] Gene Transfer/delivery and Expression of DNA and RNA Gene Transfer/delivery and Expression of DNA and RNA Sonoporation : An Efficient Technique for the Introduction of Genes into Chick Embryos(Edited by Theodore Friedmann, John Rossi) (2007)
  • Author(s): Ohta S, Ogino Y, Suzuki K, Kamimura M, Tachibana K, and Yamada G
  • Journal Title: Cold Spring Harbor Laboratory Press Pages: 711-716
  • Peer Reviewed
• [Journal Article] Journal of Comparative Neurology, Topographic organization of sensory afferents of Johnston's organ in the honeybee brain (2007)
  • Journal Title: Wiley-Liss, Inc Pages: 1030-1046
  • Peer Reviewed
• [Journal Article] A novel bubble liposome and ultrasound-mediated gene transfer to ocular surface : RC-1 cells in vitro and conjunctiva in vivo (2007)
  • Author(s): Yamashita T, Sonoda S, Suzuki R, Arimura N, Tachibana K, Maruyama K, and Sakamoto T
  • Journal Title: Experimental Eye Reserch 85(6) Pages: 741-748
  • Peer Reviewed
• [Journal Article] Biomolecular Effects of Low Intensity Ultrasound : Apoptosis, Sonotransfection, and Gene Expression (2007)
  • Author(s): Feril LB Jr, Kondo T, Tabuchi Y, Ogawa R, Zhao Q-L, Nozaki T, Yoshida T, Kudo N, and Tachibana K
  • Journal Title: Japanese Journal of Applied Physics 46(7B) Pages: 4435-4440
  • Peer Reviewed
• [Journal Article] Cessation of gastrulation is mediated by supPression of epithelial-mesenchymal transition at the ventral ectodermal ridge (2007)
  • Author(s): Ohta S, Suzuki K, Tachibana K, Tanaka H, Yamada G
  • Journal Title: Development 134 Pages: 4315-4324
  • NAID: 120002468517
  • Peer Reviewed
• [Journal Article] A novel bubble liposome and ultrasound-mediated gene transfer toocular surface: RC-1 cells in vitro and conjunctiva in vivo (2007)
  • Author(s): Yamashita T, Sonoda S, Suzuki R, Arimura N, Tachibana K, Maruyama K, and Sakamoto T
  • Journal Title: Exp Eye Res 85(6) Pages: 741-748
  • Peer Reviewed
• [Journal Article] Biomolecular Effects of Low Intensity Ultrasound: Apoptosis, Sonotransfection, and Gene Expression (2007)
  • Author(s): Feril LB Jr, Kondo T, Tabuchi Y, Ogawa R, Zhao Q-L, Nozaki T, Yoshida T, Kudo N, and Tachibana K
  • Journal Title: Japanese Journal of Applied Physics 46 Pages: 4435-4440
  • Peer Reviewed
• [Journal Article] Antitumor Effect of TNP-470, an Angiogenesis Inhibitor, Combined with Ultrasound Irradiation for Human Uterine Sarcoma Xenografts Evaluated by Contrast Color Doppler Ultrasound (2007)
  • Author(s): Emoto M, Tachibana K, Iwasaki H and Kawarabayashi T
  • Journal Title: Cancer Science 98 Pages: 929-935
  • Peer Reviewed
• [Journal Article] Inhibition of melanoma by ultrasound-microbubble-aided drug delivery suggests membrane permeabilization (2007)
  • Author(s): Sonoda S, Tachibana K, Uchino E, Yamashita T, Sakoda K, Sonoda KH, Hisatomi T, Izumi Y, Sakamoto T.
  • Journal Title: Cancer Biol Ther 6(8) Pages: 1276-1283
  • Peer Reviewed
• [Journal Article] Biomolecular effects of low intensity ultrasound : apoptosis, sonotransfection, and gene expression. (2007)
  • Author(s): Feril LB Jr, Kondo T, Tabuchi, Ogawa R, Zhao Q-L, Nozaki T, Toru Y, Kudo N, Tachibana K
  • Journal Title: Japanese Journal of Applied Physics (in press)
• [Journal Article] Bioeffects of Ultrasound for Therapy (2007)
  • Author(s): Feril LB Jr, Tachibana K, Yamaguchi K, Ogawa K, Dantsuji Y, Irie Y, Endo H
  • Journal Title: Philippine Physics Journal (in press)
• [Journal Article] Biological Effects of Ultrasound : Sonomechanical Mechanism, and Its Implications on Therapy and Biosafety (2007)
  • Author(s): Feril LB Jr, Kondo T, Tachibana K, Ogawa R, Ogawa K
  • Journal Title: Therapeutic Ultrasound : 5th International Symposium on Therapeutic Ultrasound (in press)
• [Journal Article] Emerging Technologies Using Ultrasound for Drug Delivery : Emerging Therapeutic Ultrasound (2006)
  • Author(s): Katsuro Tachibana
  • Journal Title: World Scientific Pages: 131-166
  • Peer Reviewed
• [Journal Article] Ultrasound in Medicine : To Search and diseased Tissues. (2006)
  • Author(s): Feril LB Jr, Tachibana K.
  • Journal Title: Philippine Physics journal 28 Pages: 67-72
• [Journal Article] Optimized ultrasound-mediated gene transfection in cancer cells. (2006)
  • Author(s): Feril LB Jr, Ogawa R, Tachibana K, Kondo T.
  • Journal Title: Cancer Sci. 97(10) Pages: 1111-1114
  • NAID: 10019378415
• [Journal Article] 超音波の治療への応用 (2006)
  • Author(s): 立花克郎, 入江 豊, 小川皓一
  • Journal Title: J Med Ultrasound 33(6) Pages: 631-639
  • NAID: 10020611604
• [Presentation] マイクロバブルと超音波治療 (2009)
  • Author(s): 立花克郎
  • Organizer: 第4回超音波分子診断治療研究会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-01-09
• [Presentation] マイクロバブルの治療への応用 (2008)
  • Author(s): 立花克郎
  • Organizer: 第10回国際造影超音波シンポジウム
  • Place of Presentation: 東京医科大学病院
  • Year and Date: 2008-12-13
• [Presentation] 神経超音波の基礎 (2008)
  • Author(s): 立花克郎
  • Organizer: 第11回日本栓子検出と治療学会(エンボラス学会)
  • Place of Presentation: 倉敷
  • Year and Date: 2008-11-01
• [Presentation] Low-intensity ultrasound inhibits cancer growth by modifying cell cycle (2008)
  • Author(s): FERIL Loreto
  • Organizer: 8th International Symposium on Therapeutic Ultrasound
  • Place of Presentation: Minneapolis, USA
  • Year and Date: 2008-09-12
• [Presentation] U937細胞におけるドキシルと超音波併用による殺細胞効果について (2008)
  • Author(s): 原田慶美、遠藤日富美、山口和記、立花克郎
  • Organizer: 第2回超音波分子診断治療研究会(第2回基礎技術研究会共催)
  • Place of Presentation: 北海道大学
  • Year and Date: 2008-08-08
• [Presentation] Anti-tumor Effects of Dendritic Cell-Based Cancer Immunotherapy Utilizing Novel Liposomal Bubbles and Ultrasound as Antigen Delivery System (2008)
  • Author(s): Yusuke Oda
  • Organizer: 第11回国際リボソーム研究会議
  • Place of Presentation: 横浜
  • Year and Date: 2008-07-21
• [Presentation] The Effect of Cell Killing by Ultrasound and Encapsulating Doxorubicin Bubble Liposomes(EDBL) (2008)
  • Author(s): 原田慶美
  • Organizer: 第11回国際リポソーム研究会議
  • Place of Presentation: 横浜
  • Year and Date: 2008-07-20
• [Presentation] Cancer Immunotherapy Based on Liposomal Bubbles-Mediated Antigen Delivery to Dendritic Cells (2008)
  • Author(s): Ryo Suzuki
  • Organizer: 第11回国際リボソーム研究会議
  • Place of Presentation: 横浜
  • Year and Date: 2008-07-20
• [Presentation] Catheter-based ultrasound thrombolysis (2008)
  • Author(s): Tachibana K
  • Organizer: International Sonothrombolysis Conference
  • Place of Presentation: Heidelberg, Germany
  • Year and Date: 2008-07-05
• [Presentation] Sonoporation法による皮膚への遺伝子導入 (2008)
  • Author(s): 入江豊
  • Organizer: 日本超音波学会第81回学術集会
  • Place of Presentation: 神戸
  • Year and Date: 2008-05-24
• [Presentation] バブルリポソームと超音波の併用による肝臓特異的遺伝子導入法の開発 (2008)
  • Author(s): 立花克郎
  • Organizer: 日本超音波医学会第81回学術集会
  • Place of Presentation: 神戸
  • Year and Date: 2008-05-24
• [Presentation] 超音波血栓溶解療法の臨床応用について (2008)
  • Author(s): 立花克郎
  • Organizer: 日本超音波医学会第81回学術集会
  • Place of Presentation: 神戸
  • Year and Date: 2008-05-24
• [Presentation] 超音波による眼科領域疾患に対する治療の可能性 (2008)
  • Author(s): 坂本泰二
  • Organizer: 日本超音波医学会第81回学術集会
  • Place of Presentation: 神戸
  • Year and Date: 2008-05-24
• [Presentation] 超音波・マイクロバブル・抗がん剤の併用による癌細胞株U937への殺細胞効果について (2008)
  • Author(s): 原田慶美, 立花克郎, 遠藤日富美, フェリル ロリト, 山口和記
  • Organizer: 日本超音波医学会第81回学術集会
  • Place of Presentation: 神戸
  • Year and Date: 2008-05-23
• [Presentation] Low-intensity ultrasound inhibits melanoma growth in vitro and in vivo (2008)
  • Author(s): FERIL Loreto
  • Organizer: Conjoint Meeting of XXth International Pigment Cell Conference (IPCC) & Vth International Melanoma Research Congress (IMRC)
  • Place of Presentation: Sapporo, Hokkaido
  • Year and Date: 2008-05-11
• [Presentation] Development of a novel antigen delivery method to dendritic cells with liposomal bubbles and ultrasound (2007)
  • Author(s): Ryo Suzuki, Yusuke Oda, Naoki Utoguchi, Naoki Okada, Norimitsu Kadowaki, Katsuro Tchibana, Kazuo Maruyama
  • Organizer: The 9th International Symposium on Ultrasound Contrast Imaging
  • Place of Presentation: 大阪
  • Year and Date: 2007-12-15
• [Presentation] The effect of cell killing by Ultrasound-Microbubble-Anticancer drug (2007)
  • Author(s): Yoshimi Harada, Katsuro Tachibana
  • Organizer: The 9th International Symposium on Ultrasound Contrast Imaging
  • Place of Presentation: 大阪
  • Year and Date: 2007-12-15
• [Presentation] Angiogenesis and Ultrasound-Anti-Angiogenesis Therapy in Combination with Ultrasound Irradiation for Human Cancer Evaluated by Contrast Color (2007)
  • Author(s): Makoto Emoto, Katsuro Tachibana
  • Organizer: The 9th International Symposium on Ultrasound Contrast Imaging
  • Place of Presentation: 大阪
  • Year and Date: 2007-12-15
• [Presentation] Emerging Technologies Using Ultrasound for Drug Delivery (2007)
  • Author(s): 立花克郎、フェリル ロリト
  • Organizer: International Symposium on Sonochemistry and Sonoprocessing 2007 - New horizons in Sonochemistry and Sonoprocessing
  • Place of Presentation: 京都
  • Year and Date: 2007-12-08
• [Presentation] In vivo gene delivery by sonoporation with liposomal bubbles (2007)
  • Author(s): Ryo Suzuki, Eisuke Nanami, yusuke Oda, Tomoko Takizawa, Naoki Utoguchi, Yoichi Negishi, Katsuro Tachibana, Kazuo Maruyama
  • Organizer: International Symposium on Sonochemistry and Sonoprocessing 2007-New horizonsin Sonochemistry and Sonoprocessing-
  • Place of Presentation: 京都
  • Year and Date: 2007-12-07
• [Presentation] Ultrasound-induced mechanical damage on cells enchanced by hypotoniainduced cell swelling (2007)
  • Author(s): FERIL Loreto, Tachibana Katsuro, Kondo T, Yamaguchi K
  • Organizer: 19th International Congress on Acoustics
  • Place of Presentation: Madrid Spain
  • Year and Date: 2007-09-02
• [Presentation] Effect of low-intensity ultrasound on humang astric carcinoma in nude mice (2007)
  • Author(s): Endo Hitomi, Tachibana Katsuro, Kondo T, Yamaguchi K
  • Organizer: 19th International Congress on Acoustics
  • Place of Presentation: Madrid Spain
  • Year and Date: 2007-09-02
• [Presentation] HIFU(強力集束超音波療法) (2007)
  • Author(s): 立花 克郎
  • Organizer: 第26回日本脳神経超音波学会
  • Place of Presentation: 横浜市 パシフィコ横浜
  • Year and Date: 2007-07-10
• [Presentation] Enhanced Gene Transfer by Echo Contrast Agents : Comparison between BR1 and BR14 in Vitro and in Vivo (2007)
  • Author(s): 遠藤日富美、立花克郎、フェリル ロリト
  • Organizer: 10th Annual Meeting American Society of GENE THERAPY
  • Place of Presentation: Seattle Washingon USA
  • Year and Date: 2007-06-02
• [Presentation] Sonotrans fection as a potential alternative Gene Therapy Method in treating pregnant women:A study using Gamma Interferon Gene against Neurofibromav (2007)
  • Author(s): FERIL Loreto, NAKAYAMA Juichiro, Tachibana Katsuro
  • Organizer: The23rd Fetus as a Patient International Congress
  • Place of Presentation: Manila,philippines
  • Year and Date: 2007-05-15
• [Presentation] 超音波血栓溶解療法 (2007)
  • Author(s): 立花 克郎
  • Organizer: 日本超音波医学会第80回学術集会
  • Place of Presentation: 鹿児島
• [Book] Gene Transfer delivery and Expression of DNA and RNA Edited by Theodore Friedmann John Rossi (2007)
  • Author(s): Ohta S, Ogino Y, Suzuki K, Kamimuea M, Tachibana K, Yamada G.
  • Publisher: Sonoporation:An Efficient Technique for the Introduction of Genes into Chick Embryos
• [Book] Emerging Therapeutic Ultrasound Emerging technologies using ultrasound for drag delivery (2006)
  • Author(s): Tachibana K, Tachibana S
  • Publisher: World Scientific and Imperial College Press
• [Remarks]
  • URL: http://resweb2.jhk.adm.fukuoka-u.ac.jp/FukuokaUniv/R101J_Action.do
• [Remarks]
  • URL: http://resweb2.jhk.adm.fukuoka-u.ac.jp/FukuokaUniv/R101J_Action.do