Development of femtoliter chamber for single-molecule analysis of biological reaction
| Project/Area Number |
18201025
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Nanomaterials/Nanobioscience
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| Research Institution | Osaka University |
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Principal Investigator |
NOJI Hiroyuki Osaka University, 産業科学研究所, 教授 (00343111)
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| Project Period (FY) |
2006 – 2008
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| Project Status |
Completed (Fiscal Year 2008)
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| Budget Amount *help |
¥52,130,000 (Direct Cost: ¥40,100,000、Indirect Cost: ¥12,030,000)
Fiscal Year 2008: ¥10,140,000 (Direct Cost: ¥7,800,000、Indirect Cost: ¥2,340,000)
Fiscal Year 2007: ¥20,280,000 (Direct Cost: ¥15,600,000、Indirect Cost: ¥4,680,000)
Fiscal Year 2006: ¥21,710,000 (Direct Cost: ¥16,700,000、Indirect Cost: ¥5,010,000)
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| Keywords | 超微小溶液チャンバー / 1分子計測 / 回転分子モーター / 1分子DNA解析 / 制限酵素 |
|---|
| Research Abstract |
申請者が独自に開発した超微小溶液チャンバーを用いた1分子DNA解析技術の確立を中心に研究を行った。その結果、1分子制限酵素反応、1分子DNA増幅反応、1分子F1モーター反応効率計測に成功した。また、これまでのチャンバーとは異なり、溶液の添加・回収が可能なドロプレット型のチャンバーの開発も行った。これによって、酵素反応開始や細胞培養も可能となった。
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Report
(4 results)
Research Products
(64 results)
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[Journal Article] Photo gel-sol/sol-geltransition and its patterning of a supramolecular hydrogel as stimuli-responsive biomaterials2008
Author(s)
Matsumoto S, Yamaguchi S, Ueno S, Komatsu H, Ikeda M, Ishizuka K, Iko Y, Tabata KV, Aoki H, Ito S, Noji H, Hamachi I
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Journal Title
Chemistry 14(13)
Pages: 3977-3986
Related Report
Peer Reviewed
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[Journal Article] Photo gel-sol/sol-gel transition and its patterning of a supramolecular hydrogel as stimuli-responsive biomaterials2008
Author(s)
Matsumoto S, Yamaguchi S, Ueno S, Komatsu H, Ikeda M, Ishizuka K, Iko Y, Tabata KV, Aoki H, Ito S, Noji H, Hamachi I.
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Journal Title
Chemistry 14(13)
Pages: 3977-3986
Related Report
Peer Reviewed
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[Journal Article] Temperature distribution measurement on microfabricated thermodevice for singlebiomolecular observation using fluorescent dye2006
Author(s)
Arata, H.F., Low, P., Ishizuka, K., Bergaud, C., Kim, B., Noji, H. and Fujita, H
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Journal Title
Sensors and Actuators, B : Chemical 117(2)
Pages: 339-345
Related Report
Peer Reviewed
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[Journal Article] Temperature distribution measurement on microfabricated thermodevice for single biomolecular observation using fluorescent dye.2006
Author(s)
Arata, H.F., Low, P., Ishizuka, K., Bergaud, C., Kim, B., Noji, H., Fujita, H.
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Journal Title
Sensors and Actuators, B : Chemical 117(2)
Pages: 339-345
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[Book] 生物物理2006
Author(s)
野地 博行
Total Pages
55
Publisher
日本生物物理学会
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