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Design of Base-discriminating Fluorescent Nucleosides and Their Application to Gene Analysis and SNP Genotyping

Research Project

Project/Area Number 18201026
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Nanomaterials/Nanobioscience
Research InstitutionNihon University

Principal Investigator

SAITO Isao  Nihon University, 工学部, 教授 (20026082)

Co-Investigator(Kenkyū-buntansha) SAITO Yoshio  日本大学, 工学部, 准教授 (40385985)
Project Period (FY) 2006 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥47,970,000 (Direct Cost: ¥36,900,000、Indirect Cost: ¥11,070,000)
Fiscal Year 2009: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
Fiscal Year 2008: ¥10,140,000 (Direct Cost: ¥7,800,000、Indirect Cost: ¥2,340,000)
Fiscal Year 2007: ¥12,220,000 (Direct Cost: ¥9,400,000、Indirect Cost: ¥2,820,000)
Fiscal Year 2006: ¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000)
KeywordsDNAデバイス / DNAチップ / 遺伝子検出 / 遺伝子診断 / SNP検出 / ナノバイオ / ゲノム / 蛍光分子
Research Abstract

We have designed novel base-discriminating fluorescent (BDF) nucleosides which are successfully used for SNP genotyping and gene detection. Quencher and end free molecular beacons (MB) were discovered for the first time. These low cost MB were used for the gene detection in a cell. We have also succeeded to design environmentally sensitive fluorescent nucleosides that emits strong fluorescence at different wavelengths depending upon a local environment around the nucleobase.

Report

(6 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report   Self-evaluation Report ( PDF )
  • 2007 Annual Research Report
  • 2006 Annual Research Report
  • Research Products

    (49 results)

All 2009 2008 2007 2006 Other

All Journal Article (26 results) (of which Peer Reviewed: 18 results) Presentation (10 results) Book (2 results) Remarks (4 results) Patent(Industrial Property Rights) (7 results)

  • [Journal Article] Fluorescence Switching of Photochromic Vinylpyrene Substituted 2'-Deoxyguanosine.2009

    • Author(s)
      Y. Saito, K. Matsumoto, Y. Takeuchi, S.S. Bag, S. Kodate, T. Morii, I. Saito
    • Journal Title

      Tetrahedron Lett. 50

      Pages: 1403-1406

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Ends Free and Self-quenched Molecular Beacons with Pyrene Labeled Pyrrolocitidine in the Middle of the Stem.2009

    • Author(s)
      Y. Saito, Y. Sinohara, S.S. Bag, Y. Takeuchi, K. Matsumoto, I. Saito
    • Journal Title

      Terahedron 65

      Pages: 934-934

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Ends Free and Self-quenched Molecular Beacons with Pyrene Labeled Pyrrolocitidine in the Middle of the Stem2009

    • Author(s)
      Y.Saito, Y.Sinohara, S.S.Bag, Y.Takeuchi, K.Matsumoto, I.Saito
    • Journal Title

      Terahedron 65

      Pages: 934-938

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Fluorescence Switching of Photochromic Vinylpyrene Substituted 2'-Deoxyguanosine2009

    • Author(s)
      Y.Saito, K.Matsumoto, Y.Takeuchi, S.S.Bag, S.Kodate, T.Morn, I.Saito
    • Journal Title

      Tetrahedron Lett 50

      Pages: 1403-1406

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Design of Extremely Facile 3'- and 5'- Ends Free Molecular Beacons using C8 Alkylamino Substituted 2'-Deoxyguanosine2009

    • Author(s)
      K.Matsumoto, Y.Shinohara, K.Numajiri, S.Ishioroshi, T.Morii, Y.Saito, I.Saito
    • Journal Title

      Nucleic Acids Symp Ser 53

      Pages: 141-142

    • Related Report
      2009 Annual Research Report
  • [Journal Article] C8-Alkynyl-and Alkylamino Substituted 2'-Deoxyguanosines: A Universal Linker for Nucleic Acids Modification2008

    • Author(s)
      Y. Saito, K. Matsumoto, S.S. Bag, S. Ogasawara, K. Fujimoto, K. Hanawa, I. Saito
    • Journal Title

      Tetrahedron 64

      Pages: 3578-3488

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Anthracene Based Base-discriminating Fluorescent Oligonucleotide Probes for SNPs Typing: Synthesis and Photophysical Properties2008

    • Author(s)
      Y. Saito, K. Motegi, S.S. Bag, I. Saito
    • Journal Title

      Bioorg. Med. Chem. Symposium-in-Print 16

      Pages: 107-113

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] C8-Alkynyl and alkylamino substituted 2'-deoxyguanosine2008

    • Author(s)
      Y. Saito, K. Matsumoto, S. S. Bag, K. Hanawa and I. Saito
    • Journal Title

      Tetrahedron 64

      Pages: 3478-3488

    • Related Report
      2008 Self-evaluation Report
  • [Journal Article] Anthracene based base-discriminating fluorescence ologonucleotide probe SNP2008

    • Author(s)
      Y, Saito, K. Motegi, S.S. Bag, and I. Saito
    • Journal Title

      Biooorg. Med. Chem. 16

      Pages: 107-113

    • Related Report
      2008 Self-evaluation Report
  • [Journal Article] Sysnthesis and reversible photoisomerization of photoswitchable nucleoside 8-styryll-2'-deoxyguanosine2008

    • Author(s)
      S. Ogasawara, I. Saito, and M. Maeda
    • Journal Title

      Tetrahedron Lett. 49

      Pages: 2479-2482

    • Related Report
      2008 Self-evaluation Report
  • [Journal Article] Anthracene Based Base-discriminating Fluorescent Oligonucleotide Probes for SNPs Typing : Synthesis and Photophysical Properties2008

    • Author(s)
      Y. Saito, K. Motegi, S. S. Bag, I. Saito.
    • Journal Title

      Bioorg. Med. Chem. Syaposium-in-Print 16

      Pages: 107-113

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Synthesis and Reversible Photoisomerization of Photoswitchable Nucleoside 8-Styryl-2'- deoxyguanosine2008

    • Author(s)
      S. Ogasawa, I. Saito, M. Maeda
    • Journal Title

      Tetrahedron Lett. 49

      Pages: 2479-2482

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] C8-Alkynyl- and Alkylamino Substituted 2'-Deoxyguanosines : A Universal Linker for Nucleic Acids Modification2008

    • Author(s)
      Y. Saito, K. Matsumoto, S. S Bag, S. Ogasawara, K. Fujimoto, K. Hanawa, I. Saito
    • Journal Title

      Tetrahedron 64

      Pages: 3578-3488

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Design of a Novel G-quenched Molecular Beacon; A Simple and Efficient Strategy for DNA Sequenceing Analysis2007

    • Author(s)
      Y. Saito, E. Mizuno, S.S. Bag, I. Suzuka, I. Saito.
    • Journal Title

      Chem. Commun.

      Pages: 4492-4494

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Reversible Photopadlocking on Double-stranded DNA.2007

    • Author(s)
      K. Fujimoto, S. Matsuda, Y. Yoshimura, T. Ami, I. Saito
    • Journal Title

      Chem. Commun.

      Pages: 2968-2970

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] PRODAN-Conjugated DNA: Synthesis and Photochemical Properties2007

    • Author(s)
      K. Tainaka, K. Tanaka, Ikeda, K-i., Nishiza, T. Unzai, Y. Fujiwara, I. Saito, A. Okamoto
    • Journal Title

      J. Am. Chem. Soc 129

      Pages: 4776-4784

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Synthesis and Fluorescence Properties of Dimethylaminonaphthalene-deoxyuridine Conjugates As Micropolarity-sensitive Probes.2007

    • Author(s)
      Okamoto, A. ; Tainaka, K. ; Unzai, T. ; Saito
    • Journal Title

      I. Tetrahedron 63

      Pages: 3465-3470

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Dual-labeled Oligonucleotide Probe for Sensing Adenosine via FRET2007

    • Author(s)
      Y. Saito, S. S. Bag, Y. Kusakabe, C. Nagai, K. Matsumoto, S. Kodate, I. Suzuka and I. Saito
    • Journal Title

      Chem. Commun.

      Pages: 2133-2135

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Anthracene Based Base-discriminating Fluorescent Oligonucleotide Probes for SNPs Typing2007

    • Author(s)
      Y. Saito, K. Motegi, S. S. Bag, and I. Saito
    • Journal Title

      Bioorg. Med. Chem. Symposisum-in-Print 16

      Pages: 107-113

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Design of a Novel G-quenched Molecular Beacons; A Simple and Efficient Strategy for DNA Sequencing Analysis2007

    • Author(s)
      Y. Saito, E. Mizuno, S. S. Bag, I. Suzuka and I. Saito
    • Journal Title

      Chem. Commun.

      Pages: 4492-4493

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Highly Selective Fluorescent Nucleobases for Designing Base-Discriminating Fluorescence Probe.2006

    • Author(s)
      Saito, I. ; Saito, Y. ; Hanawa, K. ; Hayashi, K. ; Motegi, K. ; Bag, S.S. ; Dohno, C. ; Ichiba, T. ; Tainaka, K. ; Okamoto
    • Journal Title

      A. Pure. Appl. Chemistry 78(12)

      Pages: 2305-2312

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Simple SNP Typing Assay Using a Base-discriminating Fluorescent Probe.2006

    • Author(s)
      A. Okamoto, K. Tainaka, Y. Ochi, K. Kanatani, I. Saito
    • Journal Title

      Molecular BioSystem. 2

      Pages: 81-81

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] DNA Hole Transport on an Electrode : Application to Effective photoelectrochemical SNP Typing.2006

    • Author(s)
      A.Okamoto, T.Kamei, I.Saito
    • Journal Title

      J. Am.Chem. Soc 128

      Pages: 658-662

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Acridone?labeled Base?discriminating Fluorescence Nuceloside2006

    • Author(s)
      Y.Saito, K.Hanawa, N.Kawasaki, S.S.Bag, I.Saito
    • Journal Title

      Chemistry Letters 35

      Pages: 1182-1183

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Intelligent Fluorescent Nucleoside in Sensing Cytosine Base2006

    • Author(s)
      S.S.Bag, Y.Saito, K.Hanawa, S.Kodate, I.Suzuka, I.SAito
    • Journal Title

      Bioorganic Med. Chem. Lett. 16

      Pages: 6338-6342

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Site?specific Transition of Cytosine to mUracil via Reversible DNA Photoligation2006

    • Author(s)
      K.Fujimoto, S.Matsuda, Y.Yoshimura, T.Matsumura, M.Hayashi, I.Saito
    • Journal Title

      Chem. Commun

      Pages: 3223-3225

    • Related Report
      2006 Annual Research Report
  • [Presentation] 両末端フリーの自己消光型モレキュラービーコンの開発2009

    • Author(s)
      齋藤義雄, 篠原雄太, 松本桂彦, 沼尻恭子, 齋藤烈
    • Organizer
      2009年光化学討論会
    • Place of Presentation
      桐生市市民文化会館
    • Year and Date
      2009-09-16
    • Related Report
      2009 Annual Research Report 2009 Final Research Report
  • [Presentation] DNA検出のためのアントラセン、ピレン骨格を有するTwinプローブの開発2009

    • Author(s)
      齋藤義雄, 鈴木梓, 篠原雄太, 石下真也, 今井和俊, 根本修克, 齋藤烈
    • Organizer
      2009年光化学討論会
    • Place of Presentation
      桐生市市民文化会館
    • Year and Date
      2009-09-16
    • Related Report
      2009 Final Research Report
  • [Presentation] 新しいSolvatofluorochromicなプリン核酸塩基のデザインと応用2009

    • Author(s)
      齋藤義雄, 高橋尚也, 松本桂彦, 篠原雄太, 竹内辰樹, 齋藤烈
    • Organizer
      2009年光化学討論会
    • Place of Presentation
      桐生市市民文化会館
    • Year and Date
      2009-09-16
    • Related Report
      2009 Final Research Report
  • [Presentation] C8位にリンカーをもつ新規グアノシン誘導体の応用2009

    • Author(s)
      齋藤義雄, 松本桂彦, 篠原雄太, 沼尻恭子, 齋藤烈
    • Organizer
      日本化学会第89回春季年会
    • Place of Presentation
      日本大学理工学部船橋キャンパス
    • Year and Date
      2009-03-27
    • Related Report
      2009 Final Research Report
  • [Presentation] ビニルピレン置換グアニン塩基を用いる蛍光スイッチの開発2009

    • Author(s)
      齋藤義雄, 竹内辰樹, 松本桂彦, 高橋尚也, 藤烈
    • Organizer
      日本化学会第89回春季年会
    • Place of Presentation
      日本大学理工学部船橋キャンパス
    • Year and Date
      2009-03-27
    • Related Report
      2009 Final Research Report
  • [Presentation] Fluorometric sensing of the B-Z DNA transition of DNA Containing C8-aIkylainino substituted 2'-deoxyguanosine2008

    • Author(s)
      松本 桂彦
    • Organizer
      第35回核酸化学国際シンポジウム
    • Place of Presentation
      京都大学吉田キャンパス
    • Year and Date
      2008-09-08
    • Related Report
      2008 Annual Research Report
  • [Presentation] Design of Dual-labeled Oligonucleotide Probes for SNPs Genotyping2007

    • Author(s)
      S. S. Bag
    • Organizer
      第5回国核酸シンポジュウム
    • Place of Presentation
      東大安田講堂
    • Year and Date
      2007-11-20
    • Related Report
      2007 Annual Research Report
  • [Presentation] アントラセン骨格を有するTwinプロープの開発と遺伝子検出への応用

    • Author(s)
      篠原雄太、鈴木梓、石下真也、齋藤義雄、齋藤烈
    • Related Report
      2008 Self-evaluation Report
  • [Presentation] C8位にリンカーを持つ新規グアノシン誘導体の応用

    • Author(s)
      松本桂彦、篠原雄太、沼尻恭子、齋藤義雄、齋藤烈、水野絵梨香、笠原竜、佐藤直樹、藤井美里
    • Related Report
      2008 Self-evaluation Report
  • [Presentation] ビニルピレン置換グアニン塩基を用いる蛍光スイッチの開発

    • Author(s)
      竹内辰樹、松本桂彦、高橋尚也、齋藤義雄、齋藤烈
    • Related Report
      2008 Self-evaluation Report
  • [Book] 化学フロンティア18ゲノム化学の最先端2007

    • Author(s)
      齋藤烈, 杉山弘, 中谷和彦共編, 化学同人
    • Related Report
      2009 Final Research Report
  • [Book] ゲノム化学の最先端2007

    • Author(s)
      齋藤烈、杉山弘、中谷和彦
    • Publisher
      化学同人
    • Related Report
      2008 Self-evaluation Report
  • [Remarks]

    • URL

      http://ch.ce.nihon-u.ac.jp/%7ei_saito/EngTop.html

    • Related Report
      2009 Annual Research Report
  • [Remarks]

    • URL

      http://ch.ce.nihon-u.ac.jp/%7ei_saito/EngTop.html

    • Related Report
      2008 Self-evaluation Report
  • [Remarks]

    • URL

      http://ch.ce.nihon-u.ac.jp/%7ei_saito/EnTop.html

    • Related Report
      2008 Annual Research Report
  • [Remarks]

    • URL

      http://ch.ce.nihon-u.ac.jp/%7ei_saito/EngToP.html

    • Related Report
      2007 Annual Research Report
  • [Patent(Industrial Property Rights)] c8位置換プリン塩基誘導体及びそれを利用した蛍光プローブ2009

    • Inventor(s)
      齋藤烈, 齋藤義雄, 高橋尚弥
    • Industrial Property Rights Holder
      日本大学
    • Acquisition Date
      2009-08-17
    • Related Report
      2009 Final Research Report
  • [Patent(Industrial Property Rights)] ステムループ構造を有する蛍光プローブ2008

    • Inventor(s)
      齋藤烈, 齋藤義雄, 松本桂彦
    • Industrial Property Rights Holder
      日本大学
    • Acquisition Date
      2008-09-02
    • Related Report
      2009 Final Research Report
  • [Patent(Industrial Property Rights)] ステムループ構造を有する蛍光プローブ2008

    • Inventor(s)
      齋藤烈、齋藤義雄、松本桂彦
    • Industrial Property Rights Holder
      日本大学、長浜バイオラボラトリ(株)
    • Filing Date
      2008-09-02
    • Related Report
      2008 Self-evaluation Report
  • [Patent(Industrial Property Rights)] ステムループ構造を有する蛍光プロープ2008

    • Inventor(s)
      齋藤烈齋藤義雄松本桂彦
    • Industrial Property Rights Holder
      日本大学
    • Filing Date
      2008-09-02
    • Related Report
      2008 Annual Research Report
  • [Patent(Industrial Property Rights)] ヌクレオチド誘導体及びその利用(FRET)2007

    • Inventor(s)
      齋藤烈, 齋藤義雄, 吉田安子, 山田和成, 丹羽孝介
    • Industrial Property Rights Holder
      日本大学
    • Acquisition Date
      2007-02-08
    • Related Report
      2009 Final Research Report
  • [Patent(Industrial Property Rights)] 水素結合性置換基を有する8―置換グアノシン誘導体及びそれを含むオリゴヌクレオチド2006

    • Inventor(s)
      齋藤烈, 齋藤義雄, 花輪和夫
    • Industrial Property Rights Holder
      日本大学
    • Filing Date
      2006-12-27
    • Related Report
      2009 Final Research Report
  • [Patent(Industrial Property Rights)] 光反応性核酸及び可逆的核酸光連結又は開裂方法2006

    • Inventor(s)
      齋藤烈, 齋藤義雄
    • Industrial Property Rights Holder
      日本大学
    • Acquisition Date
      2006-03-23
    • Related Report
      2009 Final Research Report

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Published: 2006-04-01   Modified: 2016-04-21  

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