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Development of automating completely NMR structure system that uses SAIL protein

Research Project

Project/Area Number 18207007
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Structural biochemistry
Research InstitutionTokyo Metropolitan University (2007-2008)
The Institute of Physical and Chemical Research (2006)

Principal Investigator

PETER Guentert  Tokyo Metropolitan University, 戦略研究センター, 客員教授 (20392110)

Co-Investigator(Kenkyū-buntansha) JEE Jungoo  首都大学東京, 戦略研究センター, 准教授
池 [ジュン]求  首都大学東京, 戦略研究センター, 准教授 (50381554)
Project Period (FY) 2006 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥49,010,000 (Direct Cost: ¥37,700,000、Indirect Cost: ¥11,310,000)
Fiscal Year 2008: ¥13,130,000 (Direct Cost: ¥10,100,000、Indirect Cost: ¥3,030,000)
Fiscal Year 2007: ¥14,950,000 (Direct Cost: ¥11,500,000、Indirect Cost: ¥3,450,000)
Fiscal Year 2006: ¥20,930,000 (Direct Cost: ¥16,100,000、Indirect Cost: ¥4,830,000)
KeywordsNMR / SAIL法 / NMRデータの自動帰属 / 構造計算 / NMRデータ自動解析 / タンパク質立体構造計算
Research Abstract

SAIL-FLYA法は、NMR法でのタンパク質の三次元立体構造を、人手による介在をまったく必要とせずに高精度に決定できる全自動解析システムである。SAIL-FLYA法を用いれば、従来法で必要とされるNMRスペクトルより格段に少ないデータ数でも、正確な構造決定が可能となる。本研究では、このシステムを開発、改良を加え、比較的低分子量のタンパク質であるユビキチンを例としてその性能評価を行った。加えて、さらなる性能向上のために、CYANA計算法の改良、及びFLYA法に適用可能な新たな自動化学シフト帰属法の開発等も行った。また、従来難しいかったタンパク質にSAIL-CYANA法を適用し、その構造決定に成功した。

Report

(4 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • 2006 Annual Research Report
  • Research Products

    (44 results)

All 2009 2008 2007 2006

All Journal Article (43 results) (of which Peer Reviewed: 6 results) Book (1 results)

  • [Journal Article] Protein structure determination in living cells by in-cell NMR spectroscopy2009

    • Author(s)
      Sakakibara, D., Sasaki, A., Ikeya, T., Hamatsu, J., Hanashima, T., Mishima, M., Yoshimasu, M., Hayashi, N., Mikawa, T., Walchli, M., Smith, B.O., Shirakawa, M., Guntert, P. & Ito, Y.
    • Journal Title

      Nature 458

      Pages: 102-105

    • Related Report
      2008 Final Research Report
  • [Journal Article] Solution structure of the cysteine-rich domain in Fn14, a member of the tumor necrosis factor receptor superfamily2009

    • Author(s)
      He, F., Dang, W., Saito, K., Watanabe, S., Kobayashi, N., Guntert, P., Kigawa, T, Tanaka, A., Muto, Y. & Yokoyama, S.
    • Journal Title

      Protein Sci 18

      Pages: 650-656

    • Related Report
      2008 Final Research Report
  • [Journal Article] The βE-domain of Ec-1 metallothionein : A metal-binding domain with a distinctive structure2009

    • Author(s)
      Peroza, E. A., Schmucki, R., Guntert, P., Freisinger, E. & Zerbe O.
    • Journal Title

      J. Mol. Biol 387

      Pages: 207-218

    • Related Report
      2008 Final Research Report
  • [Journal Article] Solution structure of the RNA binding domain in the human muscleblind-like protein 2.2009

    • Author(s)
      He, F., Dang, W., Abe, C., Tsuda, K., Inoue, M., Watanabe, S., Kobayashi, N., Kigawa, T, Matsuda, T., Yabuki, T., Aoki, M., Seki, E., Harada, T., Tomabechi, Y., Terada, T., Shirouzu, M., Tanaka, A., Guntert, P., Muto, Y. & Yokoyama, S.
    • Journal Title

      Protein Sci 18

      Pages: 80-91

    • Related Report
      2008 Final Research Report
  • [Journal Article] Automated structure determination from NMR Spectra2009

    • Author(s)
      Guntert, P.
    • Journal Title

      Eur. Biophys 38

      Pages: 129-143

    • Related Report
      2008 Final Research Report
  • [Journal Article] Automated assignment of NMR chemical shifts using peak-particle dynamics simulation with the DYNASSIGN algorithm2009

    • Author(s)
      Schmucki, R., Yokoyama, S. & Guntert, P.
    • Journal Title

      J. Biomol. NMR. 43

      Pages: 97-109

    • Related Report
      2008 Final Research Report
  • [Journal Article] Solution structure of the GUCT domain from human RNA helicase II/Guβ reveals the RRM fold, but implausible RNA interactions2009

    • Author(s)
      Ohnishi, S., Paakkonen, K., Koshiba, S., Tochio, N., Sato, M., Kobayashi, N., Harada, T., Watanabe, S., Muto, Y., Guntert, P., Tanaka, A., Kigawa, T., & Yokoyama, S.
    • Journal Title

      Proteins 74

      Pages: 133-144

    • Related Report
      2008 Final Research Report
  • [Journal Article] Protein structure determination in living cells by in-cell NMR spectroscopy.2009

    • Author(s)
      Sakakibara, D., Sasaki, A., Ikeya, T., Hamatsu, J., Hanashima, T., Mishima, M., Yoshimasu, M., Hayashi, N., Mikawa, T., Walchli, M., Smith, B, O., Shirakawa, M., Guntert, P. & Ito, Y.
    • Journal Title

      Nature 458

      Pages: 102-105

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Automated assignment of NMR chemical shifts using peak-partide dynamics simulation with the DYNASSIGN algorithm.2009

    • Author(s)
      Schmucki, R., Yokoyama, S. & Guntert, P.
    • Journal Title

      J. Biomol. NMR 43

      Pages: 97-109

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structure of the putative 32 kDa myrosinase binding protein from Arabidopsis (At3g16450.1)as determined by the SAIL-NMR method2008

    • Author(s)
      Takeda, M., Sugimori, N., Torizawa, T., Terauchi, T., Ono, A. M., Yagi, H., Yamaguchi, Y., Kato, K., Ikeya, T., Jee, J., Guntert, P., Aceti, D.J., Markley, J.L. & Kainosho, M.
    • Journal Title

      FEBS J. 275

      Pages: 5873-5884

    • Related Report
      2008 Final Research Report
  • [Journal Article] Structural basis of the role of the NikA ribbon-helix-helix domain in initiating bacterial conjugation2008

    • Author(s)
      Yoshida, H., Furuya, N., Lin, Y. J., Guntert, P., Komano, T. & Kainosho, M.
    • Journal Title

      J. Mol. Biol 384

      Pages: 690-701

    • Related Report
      2008 Final Research Report
  • [Journal Article] Solution structure of the extraterminal domain of the bromodomain-containing protein BRD4.2008

    • Author(s)
      Lin, Y.J., Umehara, T., Inoue, M., Saito, K., Kigawa, T., Jang, M. K., Ozato, K., Yokoyama, S., Padmanabhan, B., Guntert, P.
    • Journal Title

      Protein Sci. 17

      Pages: 2174-2179

    • Related Report
      2008 Final Research Report
  • [Journal Article] Structural basis for the selectivity of the external thioesterase of the surfactin-synthetase2008

    • Author(s)
      Koglin, A., Lohr, F., Bernhard, F., Rogov, V.R., Frueh, D.P., Strieter, E.R., Mofid, M.R., Guntert, P., Wagner, G., Walsh, C.T., Marahiel, M.A. & Dotsch, V.
    • Journal Title

      Nature 454

      Pages: 907-911

    • Related Report
      2008 Final Research Report
  • [Journal Article] The RRM domain of poly(A)-specific ribonuclease(PARN)has a non-canonical binding site for mRNA cap analog recognition2008

    • Author(s)
      Nagata, T., Suzuki, S., Endo, R., Shirouzu, M., Terada, T., Inoue, M., Kigawa, T, Guntert, P., Hayashizaki, Y., Muto, Y. & Yokoyama, S.
    • Journal Title

      Nucl. Acids Res 36

      Pages: 4754-4767

    • Related Report
      2008 Final Research Report
  • [Journal Article] Solution structure of the C-terminal dimerization domain of SARS coronavirus nucleocapsid protein determined by the SAIL-NMR method2008

    • Author(s)
      Takeda, M., Chang, C.K., Ikeya, T., Guntert, P., Chang, Y.H., Hsu, Y.L., Huang, T.H. & Kainosho, M.
    • Journal Title

      J. Mol. Biol 380

      Pages: 608-622

    • Related Report
      2008 Final Research Report
  • [Journal Article] Transmembrane segment enhanced labeling as a tool for the backbone assignment of helical membrane proteins2008

    • Author(s)
      Reckel, S., Sobhanifar, S., Schneider, B., Junge, F., Schwarz, D., Durst, F., Lohr, F., Guntert, P., Bernhard, F. & Dotsch, V.
    • Journal Title

      Proc. Natl. Acad. Sci. USA 105

      Pages: 8262-8267

    • Related Report
      2008 Final Research Report
  • [Journal Article] Solution structure of the second RNA recognition motif (RRM) domain of murine T cell intracellular antigen-1 (TIA-1) and its RNA recognition mode2008

    • Author(s)
      Kuwasako, K., Takahashi, M., Tochio, N., Abe, C., Tsuda, K., Inoue, M., Terada, T., Shirouzu, M., Kobayashi, N., Kigawa, T., Taguchi, S., Tanaka, A., Hayashizaki, Y., Guntert, P., Muto, Y. & Yokoyama, S.
    • Journal Title

      Biochemistry 47

      Pages: 6437-6450

    • Related Report
      2008 Final Research Report
  • [Journal Article] Complex assembly mechanism and an RNA-binding mode of the human p14-SF3b155 spliceosomal protein complex identified by NMR solution structure and functional analyses.2008

    • Author(s)
      Kuwasako, K., Dohmae, N., Inoue, M., Shirouzu, M., Taguchi, S., Guntert, P., Seraphin, B., Muto, Y. & Yokoyama, S.
    • Journal Title

      Proteins 71

      Pages: 1617-1636

    • Related Report
      2008 Final Research Report
  • [Journal Article] Structure of the putative 32 kDa myrosinase binding protein from Arabidopsis (At3gl6450.1)as determined by the SAIL-NMR method.2008

    • Author(s)
      Takeda, M., Sugimori, N, Torizawa, T., Terauchi, T., Ono, A. M., Yagi, H., Yamaguchi, Y., Kato, K., Ikeya, T., Jee, J., Guntert, P., Aceti, D. J, Markley, J. L. & Kainosho, M.
    • Journal Title

      FEBS J. 275

      Pages: 5873-5884

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Solution structure of the C-terminal dimerization domain of SARS coronavirus nucleocapsid protein determined by the SAIL-NMR method. Implications for RNA packaging as revealed by nucleic acid interactions2008

    • Author(s)
      Takeda, M., Chang, C. K., Ikeya, T., Guntert, P., Chang, Y. H., Hsu, Y. L., Huang, T. H. & Kainosho, M.
    • Journal Title

      Journal of Molecular Biology (in press)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] NMRによる蛋白質構造決定の自動化.In計算シミュレーションと分析データ解析2007

    • Author(s)
      Ikeya, T., Guntert, P. & Kainosho, M.
    • Journal Title

      Maruzen(inJapanese)(Tokyo)

      Pages: 148-166

    • Related Report
      2008 Final Research Report
  • [Journal Article] Automated structure determination of proteins with the SAIL-FLYA NMR method2007

    • Author(s)
      Takeda, M., Ikeya, T., Guntert, P. & Kainosho, M.
    • Journal Title

      Nature Protocols 2

      Pages: 2896-2902

    • Related Report
      2008 Final Research Report
  • [Journal Article] a package of integrated modules for systematic and interactive analysis of NMR data directed to high-throughput NMR structure studies2007

    • Author(s)
      Kobayashi, N., Iwahara, J., Koshiba, S., Tomizawa, T., Tochio, N., Guntert, P., Kigawa, T. & Yokoyama, S. KUJIRA
    • Journal Title

      J. Biomol. NMR 39

      Pages: 31-52

    • Related Report
      2008 Final Research Report
  • [Journal Article] Structural insight into dimeric interaction of the SARAH domains from Mst1 and RASSF family proteins in the apoptosis pathway2007

    • Author(s)
      Hwang, E., Ryu, K.S., Paakkonen, K., Guntert, P., Cheong, H.K., Lim, D.S., Lee, J.O., Jeon, Y.H. & Cheong, C
    • Journal Title

      Proc. Natl. Acad. Sci. USA 104

      Pages: 9236-9241

    • Related Report
      2008 Final Research Report
  • [Journal Article] Solution structure of an atypical WW domain in a novel β-clam-like dimeric form2007

    • Author(s)
      Ohnishi, S., Guntert, P., Koshiba, S., Tomizawa, T., Akasaka, R., Tochio, N., Sato, M., Inoue, M., Harada, T., Watanabe, S., Tanaka, T., Shirouzu, M., Kigawa, T. & Yokoyama, S.
    • Journal Title

      FEBS Lett 581

      Pages: 462-468

    • Related Report
      2008 Final Research Report
  • [Journal Article] Automated structure determination of proteins with the SAIL-FLYA NMR method.2007

    • Author(s)
      Takeda, M., Ikeya, T., Guntert, P. & Kainosho, M.
    • Journal Title

      Nature Protocols 2

      Pages: 2896-2902

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structural insight into dimeric interaction of the SARAH domains from Mst1 and RASSF family proteins in the apoptosis pathway2007

    • Author(s)
      Hwang, E., Ryu, K. S., Paakkonen, K., Guntert, P., Cheong, H. K., Lim, D. S., Lee, J. O., Jeon, Y. H. & Cheong, C.
    • Journal Title

      Proc. Natl. Acad. Sci. USA 104

      Pages: 9236-9241

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A photoswitchable miniprotein based on the sequence of avian pancreatic polypeptide2006

    • Author(s)
      Jurt, S., Aemissegger, A., Guntert, P., Zerbe, O. & Hilvert, D.
    • Journal Title

      Angew. Chem. Int. Ed. 45

      Pages: 6297-6300

    • Related Report
      2008 Final Research Report
  • [Journal Article] Solution structures of the SURP domains and the subunit-assembly mechanism within the splicing factor SF3a complex in 17S U2 snRNP2006

    • Author(s)
      Kuwasako, K., He, F., Inoue, M., Tanaka, A., Sugano, S., Guntert, P., Muto, Y. & Yokoyama, S.
    • Journal Title

      Structure 14

      Pages: 1677-1689

    • Related Report
      2008 Final Research Report
  • [Journal Article] Automated protein structure determination from NMR spectra2006

    • Author(s)
      Lopez-Mendez, B. & Guntert, P.
    • Journal Title

      J. Am. Chem. Soc. 128

      Pages: 13112-13122

    • Related Report
      2008 Final Research Report
  • [Journal Article] Solution structures of the first and fourth TSR domains of F-spondin2006

    • Author(s)
      Paakkonen, K., Tossavainen, H., Permi, P., Rakkolainen, H., Rauvala, H., Raulo, E., Kilpelainen, I. & Guntert, P.
    • Journal Title

      Proteins 64

      Pages: 665-672

    • Related Report
      2008 Final Research Report
  • [Journal Article] 高分子量タンパク質のNMR構造決定-より速く、より正確に : タンパク質NMRの常識を覆す革新技術SAIL法2006

    • Author(s)
      Kainosho, M. & Guntert, P.
    • Journal Title

      Stereo-array isotope labeling for faster and more accurate NMR structure determinations of high molecular weight proteins Biotechnology J. (in Japanese)(Tokyo) 6

      Pages: 467-470

    • Related Report
      2008 Final Research Report
  • [Journal Article] Fully automated structure determinations of the Fes SH2 domain using different sets of NMR spectra2006

    • Author(s)
      Scott, A., Lopez-Mendez, B. & Guntert, P.
    • Journal Title

      Magn. Reson. Chem. 44

    • Related Report
      2008 Final Research Report
  • [Journal Article] Evaluation of stereo-array isotope labeling (SAIL) patterns for automated structural analysis of proteins with CYANA.2006

    • Author(s)
      Ikeya, T., Terauchi, T., Guntert, P., Kainosho, M.
    • Journal Title

      Magn. Reson. Chem 44

    • Related Report
      2008 Final Research Report
  • [Journal Article] Solution structure of the antifreeze-like domain of human sialic acid synthase2006

    • Author(s)
      Hamada, T., Ito, Y., Abe, T., Hayashi, F., Guntert, P., Inoue, M., Kigawa, T., Terada, T., Shirouzu, M., Yoshida, M., Tanaka, A., Sugano, S., Yokoyama, S. & Hirota, H.
    • Journal Title

      Protein Sci. 15

      Pages: 1010-1016

    • Related Report
      2008 Final Research Report
  • [Journal Article] NMR structure of the R-module-A parallel β-roll subunit from an Azotobacter vinelandii mannuronan C-5 epimerase2006

    • Author(s)
      Aachmann, F.L., Svanem, B.I. G., Guntert, P., Petersen, S.B., Valla, S. & Wimmer, R.
    • Journal Title

      J. Biol. Chem 281

      Pages: 7350-7356

    • Related Report
      2008 Final Research Report
  • [Journal Article] Optimal isotope labeling for NMR protein structure determinations2006

    • Author(s)
      Kainosho, M., Torizawa, T., Iwashita, Y., Terauchi, T., Ono, A.M. & Guntert, P.
    • Journal Title

      Nature 440

      Pages: 52-57

    • Related Report
      2008 Final Research Report
  • [Journal Article] Symbolic NMR product operator calculations2006

    • Author(s)
      Guntert, P.
    • Journal Title

      Int. J. Quant. Chem 106

      Pages: 344-350

    • Related Report
      2008 Final Research Report
  • [Journal Article] Optimal isotope labelling for NMR protein structure determinations2006

    • Author(s)
      Kainosho, M., Torizawa, T., Iwashita, Y., Terauchi, T., Ono, A., M., Gontert, P.
    • Journal Title

      Nature 440

      Pages: 52-57

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Evaluation of stereo-array isotope labeling (SAIL) patterns for automated structural analysis of proteins with CYANA2006

    • Author(s)
      Ikeya, T., Terauchi, T., Guntert, P., Kainosho, M.
    • Journal Title

      Magnetic Resonance in Chemistry 44

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Automated protein structure determination from NMR spectra2006

    • Author(s)
      Lopez-Mendez, B., Guntert, P.
    • Journal Title

      Journal of the American Chemical Society 128

      Pages: 13112-13122

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Fully automated structure determinations of the Fes SH2 domain using different sets of NMR spectra2006

    • Author(s)
      Scott, A., Lopez-Mendez, B., Guntert, P.
    • Journal Title

      Magnetic Resonance in Chemistry 44

    • Related Report
      2006 Annual Research Report
  • [Journal Article] 高分子量タンパク質のNMR構造決定-より速く、より正確に : タンパク質NMRの常識を覆す革新技術SAIL法2006

    • Author(s)
      甲斐荘 正恒, Guntert, P.
    • Journal Title

      バイオテクノロジージャーナル 6

      Pages: 467-470

    • Related Report
      2006 Annual Research Report
  • [Book] 計算シミュレーションと分析データ解析(分担執筆項目:NMRによる蛋白質構造決定の自動化)2007

    • Author(s)
      池谷 鉄兵, Guntert Peter, 甲斐荘 正恒(分担執筆者)
    • Total Pages
      18
    • Publisher
      丸善株式会社
    • Related Report
      2007 Annual Research Report

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Published: 2006-04-01   Modified: 2016-04-21  

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