Mechanism for heterochromatin formation depending on non-coding RNA and RNAi

• Project/Area Number: 18207012
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Molecular biology
• Research Institution: Hokkaido University (2008); Kyoto University (2006-2007)
• Principal Investigator: MURAKAMI Yota Hokkaido University, 大学院・先端生命科学研究院, 教授 (20260622)
• Co-Investigator(Kenkyū-buntansha): TANAKA Katsunori 関西学院大学, 理工学部, 教授 (60273926)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥49,400,000 (Direct Cost: ¥38,000,000、Indirect Cost: ¥11,400,000); Fiscal Year 2008: ¥14,430,000 (Direct Cost: ¥11,100,000、Indirect Cost: ¥3,330,000); Fiscal Year 2007: ¥14,430,000 (Direct Cost: ¥11,100,000、Indirect Cost: ¥3,330,000); Fiscal Year 2006: ¥20,540,000 (Direct Cost: ¥15,800,000、Indirect Cost: ¥4,740,000)
• Keywords: クロマチン / non-coding RNA,RNA干渉 / ヘテロクロマチン / RNAi / 分裂酵母 / non-coding RNA / siRNA / サイレンシング

Research Abstract

分裂酵母のヘテロクロマチンはヘテロクロマチン内で転写されるnon-coding RNA(ncRNA)及びRNAi機構に依存して形成される。この系がどのように成り立ち制御されているのか、分子遺伝学的、分子生物学的手法を駆使して解析をおこなった。その結果ncRNAを転写するRNAポリメラーゼIIがこのシステムで果たす役割を明確にするともに、ヘテロクロマチン内でのncRNA転写制御機構の一端を明らかにした。さらにこのシステムで機能する新規因子の同定に成功した。

Research Products

• [Journal Article] Dynamic regulation of heterochromatin via phosphorylation of HP1-family proteins (2010)
  • Author(s): Shimada A., Murakami Y.
  • Journal Title: Epigenetics 5 Pages: 30-33
• [Journal Article] Phosphorylation of Swi6/HP1 regulates transcriptional gene silencing at heterochromatin (2009)
  • Author(s): Shimada A., Dohke K., Sadaie M., Shinmyozu, K., Nakayama, J-I., Urano, T., Murakami, Y.
  • Journal Title: Genes & Dev 23 Pages: 18-23
• [Journal Article] Phosphorylation of Swi6/HP1 regulates transcriptional gene-silencing at heterochromatin (2009)
  • Author(s): Shimada, et al.
  • Journal Title: Genes & Dev. 23 Pages: 18-23
  • Peer Reviewed
• [Journal Article] Fission yeast chromatin assembly factor 1 assists in the replication-coupled maintenance of heterochromatin (2008)
  • Author(s): Dohke K., S. Miyazaki, Tanaka, K., Urano, T., Grewal, S., Murakami, Y.
  • Journal Title: Genes to Cells 13 Pages: 1027-43
• [Journal Article] Schizosaccharomyces pombe Orc5 plays multiple roles in the maintenance of genome stability throughout the cell cycle (2008)
  • Author(s): Kato H., Matsunaga, F., Miyazaki S., Yin, L., D'Urso, G, Tanaka, K., Murakami, Y.
  • Journal Title: Cell Cycle 7 Pages: 1083-1094
• [Journal Article] Schizosaccharomyces pombe Orc5 plays multiple roles in the maintenance of genome stability throughout the cell cycle (2008)
  • Author(s): Kato, et al
  • Journal Title: Cell Cycle 7 Pages: 1083-1094
  • Peer Reviewed
• [Journal Article] Fission yeast chromatin assembly factor 1 assists in the replication-coupled maintenance of heterochromatin (2008)
  • Author(s): Dohke, et al.
  • Journal Title: Gene to Cells 13 Pages: 1027-1043
  • Peer Reviewed
• [Journal Article] Schizosaccharomyces pombe Orc5 plays multiple roles in the maintenance of genome stability throughout the cell (2008)
  • Author(s): Kato H., et. al.
  • Journal Title: Cell Cycle (in press)
  • Peer Reviewed
• [Journal Article] The novel genemus7^+ is involved in the repair of replication-associated DNA damage in fission yeast (2007)
  • Author(s): Yokoyama, M., Inoue H., Ishii, C., Murakami Y.
  • Journal Title: DNA repair 6 Pages: 770-780
• [Journal Article] The Cenp-B homolog, Abp1, interacts with the initiation protein Cdc23 and is required for efficient DNA replication in fission yeast (2007)
  • Author(s): Locovei, A., Spiga M. -G., Tanaka T., Murakami, Y., D'Urso, G.
  • Journal Title: Cell division 1 Pages: 27-27
• [Journal Article] Faster and easier chromatin immunoprecipitation assay with high sensitivity (2007)
  • Author(s): Kohzaki H., Murakami Y.
  • Journal Title: Proteomics 4 Pages: 10-14
• [Journal Article] Transscription factor, Runx1 recruits the polyomavirus replication origin to replication factories (2007)
  • Author(s): Murakami, Y., Chen, L. -F., Sanechika, N., Kohzaki, H., Ito, Y.
  • Journal Title: J. Cell. Biochem 100 Pages: 1313-1323
• [Journal Article] Faster and easier chromatin immunoprecipitation assay with high sensitivity. (2007)
  • Author(s): Kohzaki et al.
  • Journal Title: Proteomics 4 Pages: 10-14
• [Journal Article] Transscription factor, Runxl recruits the polyomavirus replication origin to replication factories. (2007)
  • Author(s): Murakami et al.
  • Journal Title: J. Cell. Biochem 100 Pages: 1313-1323
• [Journal Article] The novel gene mus7+ is involved in the repair of replication-associated DNA damage in fission yeast (2007)
  • Author(s): Yokoyama et al.
  • Journal Title: DNA repair (in press)
• [Journal Article] Runx3 expression during mouse tongue and palate development. (2006)
  • Author(s): Yamamoto et al.
  • Journal Title: Discov Mol Cell Evol Biol. 1 Pages: 695-699
• [Journal Article] The Cenp-B homolog, Abpl, interacts with the initiation protein Cdc23 and is required for efficient DNA replication in fission yeast. (2006)
  • Author(s): Locovei, A. et al.
  • Journal Title: Cell Division 1 Pages: 27-27
• [Presentation] Regulation of heterochromatin function by phosphorylation (2008)
  • Author(s): 村上洋太
  • Organizer: Asia-Pacific Regional S.pombe Meeting
  • Place of Presentation: Singapore
• [Presentation] Regulation of heterochromatin function by phosphorylation (2008)
  • Author(s): 村上洋太
  • Organizer: EMBO Workshop : Chromosome segregation : Centromeres & Kinetochores
  • Place of Presentation: Archachoh, Bordeaux, France
• [Presentation] RNAiに依存するヘテロクロマチン形成における non-coding RNAの転写とダイナミクス (2008)
  • Author(s): 村上洋太
  • Organizer: 第2回日本エピジェネティクス研究会年会
  • Place of Presentation: 静岡県、三島
• [Presentation] Determinants of the fate of non-coding RNA transcribed from RNAi-dependent heterochromatin in fission yeast" (2007)
  • Author(s): 村上洋太
  • Organizer: Keystone Symposia 2007 0n Epigenetics
  • Place of Presentation: Breckenridge, CO, USA
  • Year and Date: 2007-04-12