The role of mitochondria in programmed cell death in higher plants

• Project/Area Number: 18208002
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Breeding science
• Research Institution: The University of Tokyo
• Principal Investigator: TSUTSUMI Nobuhiro The University of Tokyo, 大学院・農学生命科学研究科, 教授 (00202185)
• Co-Investigator(Kenkyū-buntansha): NAKAZONO Mikio 東京大学, 大学院・農学生命科学研究科, 准教授 (70282697) ; ARIMURA Shinichi 東京大学, 大学院・農学生命科学研究科, 助教 (00396938)
• Project Period (FY): 2005 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥45,890,000 (Direct Cost: ¥35,300,000、Indirect Cost: ¥10,590,000); Fiscal Year 2008: ¥12,480,000 (Direct Cost: ¥9,600,000、Indirect Cost: ¥2,880,000); Fiscal Year 2007: ¥12,220,000 (Direct Cost: ¥9,400,000、Indirect Cost: ¥2,820,000); Fiscal Year 2006: ¥21,190,000 (Direct Cost: ¥16,300,000、Indirect Cost: ¥4,890,000)
• Keywords: タペート / ミトコンドリア分裂 / ELM1 / タペート細胞 / Kaede / 多光子レーザー顕微鏡 / 花粉形成 / ミトコンドリアダイナミクス / 雄性不稔 / ミトコンドリア融合 / CCCP / Dendra2

Research Abstract

植物に特有のミトコンドリア分裂因子ELM1 を新たに同定した.このタンパク質は, ミトコンドリア分裂時に分裂装置の一つであるダイナミン様タンパク質DRP3をミトコンドリアへリクルートする機能を持っていた.花粉成熟の過程でタペート組織が崩壊する際に, ミトコンドリアが約室内に放出されることを新たに見出した.

Research Products

• [Journal Article] Arabidopsis dynamin-related proteins DRP3Aand DRP3B are functionally redundant inmitochondrial fission, but have distinct rolesin peroxisomal fission (2009)
  • Author(s): Fujimoto, M., Arimura, S., Mano, S., Kondo, M.,Saito, C., Ueda, T., Nakazono, M., Nakano,A., Nishimura, M., and Tsutsumi, N
  • Journal Title: Plant Journal 58 Pages: 388-400
• [Journal Article] Functional association of cell death suppressor, Arabidopsis Bax inhibitor-1 (2009)
  • Author(s): Nagano, M., Ihara-Ohori, Y., Imai, H., Inada, N.,Fujimoto, M., Tsutsumi, N., Uchimiya, H.,and Kawai-Yamada, M
  • Journal Title: with fatty acid 2-hydroxylation through cytochrome b(5). Plant Journal 58 Pages: 122-134
• [Journal Article] Arabidopsis dynamin-related proteins DRP3A and DRP3B are functionally redundant in mitochondrial fission, but have distinct roles in peroxisomal fission. (2009)
  • Author(s): Fujimoto,M
  • Journal Title: Plant J. (In Press)
  • Peer Reviewed
• [Journal Article] Functional association of cell death suppressor,Arabidopsis Bax inhi bitor-1,with fatty acid 2-hydroxylation through cytochrome b(5) (2009)
  • Author(s): Nagano M
  • Journal Title: Plant J. 58 Pages: 122-134
  • Peer Reviewed
• [Journal Article] Arabidopsis ELONGATED MITOCHONDRIA1 is required for localization of DYNAMIN-RELATED PROTEIN3A to mitochondrial fission sites (2008)
  • Author(s): Arimura, S., Fujimoto, M., Doniwa, Y., oya,N.,Nakazono, M., Sakamoto, W., and Tsutsumi, N.
  • Journal Title: Plant Cell 20 Pages: 1555-1566
• [Journal Article] Arabidopsis dynamin-related protein DRP2B is co-localized with DRP1A on the leading edge of the forming cell plate (2008)
  • Author(s): Fujimoto, M., Arimura, S.I., Nakazono, M., and Tsutsumi, N.
  • Journal Title: Plant Cell Reports 27 Pages: 1581-1586
• [Journal Article] Various Spatiotemporal Expression Profiles of Anther-Expressed Genes in Rice (2008)
  • Author(s): Hobo, T., Suwabe, K., Aya, K., Suzuki, G., Yano,K., Ishimizu, T., Fujita, M., Kikuchi, S.,Hamada, K., Miyano, M., Fujioka, T., Kaneko,F., Kazama, T., Mizuta, Y., Takahashi, H.,Shiono, K., Nakazono, M., Tsutsumi, N.,Nagamura, Y., Kurata, N., Watanabe, M., and Matsuoka, M
  • Journal Title: Plant and Cell Physiology 49 Pages: 1417-1428
  • NAID: 10027333889
• [Journal Article] Transfer of rice mitochondrial ribosomal protein L6 gene to the nucleus : acquisition of the 5'-untranslated region via a transposable element (2008)
  • Author(s): Kubo, N., Fujimoto, M., Arimura, S., Hirai, M., and Tsutsumi, N
  • Journal Title: Bmc Evolutionary Biology 8
• [Journal Article] Mitochondrial dynamics in plant male gametophyte visualized by fluorescent live imaging (2008)
  • Author(s): Matsushima, R., Hamamura, Y., Higashiyama, T.,Arimura, S., Sodmergen, Tsutsumi, N., and Sakamoto, W
  • Journal Title: Plant and Cell Physiology 49 Pages: 1074-1083
  • NAID: 10027332435
• [Journal Article] The intermediate stages of chloroplast rpl32 gene transfer in Malpighiales (2008)
  • Author(s): Roy, S., Ueda, M., Murata, J., and Tsutsumi, N
  • Journal Title: Genes & Genetic Systems 83 Pages: 496-496
• [Journal Article] Separated Transcriptomes of Male Gametophyte and Tapetum in Rice : Validity of a Laser Microdissection(LM)Microarray (2008)
  • Author(s): Suwabe, K., Suzuki, G., Takahashi, H., Shiono,K., Endo, M., Yano, K., Fujita, M., Masuko,H., Saito, H., Fujioka, T., Kaneko, F., Kazama,T., Mizuta, Y., Kawagishi-Kobayashi, M.,Tsutsumi, N., Kurata, N., Nakazono, M., and Watanabe, M
  • Journal Title: Plant and Cell Physiology 49 Pages: 1407-1416
  • NAID: 10027333829
• [Journal Article] Identification of the OsOPR7 gene encoding 12-oxophytodienoate reductase involved in the biosynthesis of jasmonic acid in rice (2008)
  • Author(s): Tani, T., Sobajima, H., Okada, K., Chujo, T.,Arimura, S.I., Tsutsumi, N., Nishimura, M.,Seto, H., Nojiri, H., and Yamane, H
  • Journal Title: Planta 227 Pages: 517-526
• [Journal Article] Presence of a latent mitochondrial targeting signal in gene on mitochondrial genome (2008)
  • Author(s): Ueda, M., Fujimoto, M., Arimura, S.I., Tsutsumi,N., and adowaki, K.I
  • Journal Title: Molecular Biology and Evolution 25 Pages: 1791-1793
• [Journal Article] Substitution of thegene for chloroplast RPS16 was assisted by generation of a dual targeting signal (2008)
  • Author(s): Ueda, M., Nishikawa, T., Fujimoto, M., Takanashi, H., Arimura, S., Tsutsumi, N., and Kadowaki, K
  • Journal Title: Molecular Biology and Evolution 25 Pages: 1566-1575
• [Journal Article] Arabidopsis ELONGATED MITOCHONDRIAI is required for localization of DYNAMIN-RELATED PROTEIN3A to mitochondrial fission sites. (2008)
  • Author(s): Arimura S
  • Journal Title: Plant Cell 20 Pages: 1555-1566
  • Peer Reviewed
• [Journal Article] Substitution of the gene for chloroplast RPS16 was assisted by generation of a dual targeting signal. (2008)
  • Author(s): Ueda M
  • Journal Title: Mol Biol Evol. 25 Pages: 1566-1575
  • Peer Reviewed
• [Journal Article] Various spatiotemporal expression profiles of anther-expressed genes in rice. (2008)
  • Author(s): Hobo T
  • Journal Title: Plant Cell Physiol. 49 Pages: 1417-1428
  • Peer Reviewed
• [Journal Article] Separated transcriptomes of male gametophyte and tapetum in rice:validity of a laser microdissection (LM) microarray. (2008)
  • Author(s): Suwabe K
  • Journal Title: Plant Cell Physiol 49 Pages: 1407-1406
  • Peer Reviewed
• [Journal Article] Mitochondrial dynamics in plant male gametophyte visualized by fluorescent live imaging. (2008)
  • Author(s): Matushima R
  • Journal Title: Plant Cell Physiol 49 Pages: 1074-1083
  • Peer Reviewed
• [Journal Article] Arabidopsis dynamin-related protein DRP2B is co-lacalized with DRPIA on the leading edge of the forming cell plate (2008)
  • Author(s): Fujimoto M
  • Journal Title: Plant Cell Rep. 27 Pages: 1581-1586
  • Peer Reviewed
• [Journal Article] Presence of a latent mitochondrial targeting signal in gene on mitochondrial genome (2008)
  • Author(s): Ueda M
  • Journal Title: Mol. Biol. Evol. 25 Pages: 1791-1793
  • Peer Reviewed
• [Journal Article] Identification of the OsOPR7 gene encoding 12-oxophytodienoate reductase involved in the biosynthesis of jasmonic acid in rice (2008)
  • Author(s): Tani T
  • Journal Title: Planta 227 Pages: 517-526
  • Peer Reviewed
• [Journal Article] Ethylene promotes submergence-induced expression of OsABA8oxl (2007)
  • Author(s): Saika, H., Okamoto, M., Kushiro, T., Shinoda, S.,Jikumaru, Y., Fujimoto, M., Miyoshi, K.,Arikawa, T., Arimura, S.I., Kamiya, Y.,Tsutsumi, N., Nambara, E., and Nakazono, M.
  • Journal Title: a gene that encodes ABA 8'-hydroxylase in rice
• [Journal Article] Ethylene promotes submergence-induced expression of OsABA8ox1 (2007)
  • Author(s): Saika, H., Okamoto, M., Miyoshi, K., Kushiro, T.,Shinoda, S., Jikumaru, Y., Fujimoto, M.,Arikawa, T., Takahashi, H., Ando, M.,Arimura, S., Miyao, A., Hirochika, H.,Kamiya, Y., Tsutsumi, N., Nambara, E., and Nakazono, M
  • Journal Title: a gene that encodes ABA 8 '-hydroxylase in rice. Plant and Cell Physiology 48 Pages: 287-298
• [Journal Article] Expression profiling in the coleoptile of rice reduced adh activity (rad) mutant using Laser Microdissection (2007)
  • Author(s): Takahashi, H., Saika, H., Matsumura, H.,Nishizawa, N.K., Tsutsumi, N., and Nakazono,M
  • NAID: 130006990680
• [Journal Article] The structure of the DNA molecules which constitute mitochondrial genome of Arabidopsis thaliana L (2007)
  • Author(s): Takanashi, H., Arimura, S.I., and Tsutsumi, N
  • NAID: 130006990517
• [Journal Article] Loss of the rp132 gene from the chloroplast genome and subsequent acquisition of a preexisting transit peptide within the nuclear gene in Populus (2007)
  • Author(s): Ueda, M., Fujimoto, M., Arimura, S.I., Murata, J.,Tsutsumi, N., and Kadowaki, K.I
  • Journal Title: Gene 402 Pages: 51-56
• [Journal Article] Single-organelle tracking by two-photon conversion (2007)
  • Author(s): Watanabe, W., Shimada, T., Matsunaga, S.,Kurihara, D., Fukui, K., Arimura, S.,Tsutsumi, N., Isobe, K., and Itoh, K.
  • Journal Title: Optics Express 15 Pages: 2490-2498
• [Journal Article] Rice tillering dwarf mutant dwarf3 has increased leaf longevity during darkness-induced senescence or hydrogen peroxide-induced cell death (2007)
  • Author(s): Yan, H., Saika, H., Maekawa, M., Takamure, I.Tsutsumi, N., Kyozuka, J., and Nakazono, M
  • Journal Title: Genes & Genetic Systems 82 Pages: 361-366
  • NAID: 10024394884
• [Journal Article] Mitochonria use action filaments as rails for fast translocation in Arabidopsis and tobacco cells (2007)
  • Author(s): Doniwa Y., Arimura SI., Tsutsumi N.
  • Journal Title: Plant Biotech 24 Pages: 441-447
  • Peer Reviewed
• [Journal Article] Imaging of plant dynamin-related proteins and clathrin around the plasma membrane by variable incidence angle fluorescence microscopy (2007)
  • Author(s): Fujimoto M., Arimura SI., Nakazono M., Tsutsumi N.
  • Journal Title: Plant Biotech 24 Pages: 449-455
  • NAID: 10021912079
  • Peer Reviewed
• [Journal Article] Loss of the rpl32 gene from the chloroplast genome and subsequent acquisition of a preexisting transit peptide within the nuclear gene in Populus (2007)
  • Author(s): Ueda M., Fujimoto M., Arimtira SI., Murata J., Tsutsumi N., Kadowaki KI.
  • Journal Title: Gene 402 Pages: 51-56
  • Peer Reviewed
• [Journal Article] Single-organelle tracking by two-photon conversion (2007)
  • Author(s): Watanabe, W. et al.
  • Journal Title: Optics Express 15 Pages: 2490-2498
• [Journal Article] Isolation of Arabidopsis mutants with defect in mitochondrial morphology. (2006)
  • Author(s): Arimura, S.I., Feng, X.G., Sakamoto, W., and Tsutsumi, N
  • Journal Title: Genes & Genetic Systems 81 Pages: 451-451
• [Journal Article] Isolation and characterization of the pea cytochrome c oxidase Vb gene (2006)
  • Author(s): Kubo, N., Arimura, S., Tsutsumi, N., Kadowaki,K., and Hirai, M
  • Journal Title: Genome 49 Pages: 1481-1489
• [Journal Article] Analysis of expression of genes for mitochondrial aldehyde dehydrogenase in maize during submergence and following re-aeration (2006)
  • Author(s): Meguro, N., Tsuji, H., Suzuki, Y., Tsutsumi, N.,Hirai, A., and Nakazono, M
  • Journal Title: Breeding Science 56 Pages: 365-370
  • NAID: 110004863708
• [Journal Article] A point mutation of Adh1 gene is involved in the repression of coleoptile elongation under submergence in rice (2006)
  • Author(s): Saika, H., Matsumura, H., Takano, T., Tsutsumi,N., and Nakazono, M
  • Journal Title: Breeding Science 56 Pages: 69-74
  • NAID: 110004361894
• [Journal Article] Conservation and diversification of meristem maintenance mechanism in Oryza sativa : Function of the FLORAL ORGAN NUMBER2 gene (2006)
  • Author(s): Suzaki, T., Toriba, T., Fujimoto, M., Tsutsumi, N.,Kitano, H., and Hirano, H.Y
  • Journal Title: Plant and Cell Physiology 47 Pages: 1591-1602
  • NAID: 10018809272
• [Journal Article] Different amounts of DNA in each mitochondrion in rice root (2006)
  • Author(s): Takanashi, H., Arimura, S., Sakamoto, W., and Tsutsumi, N
  • Journal Title: Genes & Genetic Systems 81 Pages: 215-218
  • NAID: 10018212814
• [Journal Article] Dynamic and reversible changes in histone H3-Lys4 methylation and H3 acetylation occurring at submergence-inducible genes in rice (2006)
  • Author(s): Tsuji, H., Saika, H., Tsutsumi, N., Hirai, A., and Nakazono, M
  • Journal Title: Plant and Cell Physiology 47 Pages: 995-1003
  • NAID: 10018185920
• [Journal Article] Evidence for transit peptide acquisition through duplication and subsequent frameshift mutation of a preexisting protein gene in rice (2006)
  • Author(s): Ueda, M., Fujimoto, M., Arimura, S., Tsutsumi,N., and Kadowaki, K
  • Journal Title: Molecular Biology and Evolution 23 Pages: 2405-2412
• [Journal Article] Promoter shuffling at a nuclear gene for mitochondrial RPL27 (2006)
  • Author(s): Ueda, M., Arimura, S., Yamamoto, M.P., Takaiwa,F., Tsutsumi, N., and Kadowaki, K
  • Journal Title: Involvement of interchromosome and subsequent intrachromosome recombinations. Plant Physiology 141 Pages: 702-710
• [Journal Article] The mitochondrial fission regulator DRP3B does not regulate cell death in plants (2006)
  • Author(s): Yoshinaga, K., Fujimoto, M., Arimura, S., Tsutsumi, N., Uchimiya, H., and Kawai-Yamada, M
  • Journal Title: Annals of Botany 97 Pages: 1145-1149
• [Journal Article] Isolation and characterization of the pea cytochrome c oxidase Vb gene (2006)
  • Author(s): Kubo, N. et al.
  • Journal Title: Genome 49 Pages: 1481-1489
• [Journal Article] Different amounts of DNA in each mitochondrion in rice root (2006)
  • Author(s): Takanashi, H. et al.
  • Journal Title: Genes and Genetic Systems 81 Pages: 215-218
• [Journal Article] The mitochondrial fission regulator DRP3B does not regulate cell death in plants (2006)
  • Author(s): Yoshinaga, K. et al.
  • Journal Title: Annals of Botany 97 Pages: 1145-1149
• [Journal Article] Promoter shuffling at a nuclear gene for mitochondrial RPL27. Involvement of interchromosome and subsequent intrachromosome recombinations (2006)
  • Author(s): Ueda, M. et al.
  • Journal Title: Plant Physiology 141 Pages: 702-710
• [Journal Article] Evidence for transit peptide acquisition through duplication and subsequent frameshift mutation of a preexisting protein gene in rice (2006)
  • Author(s): Ueda, M. et al.
  • Journal Title: Molecular Biology and Evolution 23 Pages: 2405-2412
• [Presentation] Plant mitochondrial dynamics;fission,fUsion,and their related genes (2007)
  • Author(s): Arimura SI., Tsutsumi N,
  • Organizer: International Congress on Plant Mitochondrial Biology
  • Place of Presentation: 奈良