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Stabilization of the life cycle of temperate fruit trees under irregular climates - elucidation of the molecular mechanism on water movement and redox regulation in the dormant buds

Research Project

Project/Area Number 18208003
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Horticulture/Landscape architecture
Research InstitutionUniversity of Tsukuba

Principal Investigator

GEMMA Hiroshi  University of Tsukuba, 大学院・生命環境科学研究科, 教授 (70094406)

Co-Investigator(Kenkyū-buntansha) SUGAYA Sumiko  筑波大学, 大学院・生命環境科学研究科, 准教授 (90302372)
SEKOZAWA Yoshihiko  筑波大学, 大学院・生命環境科学研究科, 助教 (90361310)
YOSHIDA Mitsuru  農業・食品産業技術総合研究機構, 食品総合研究所・食品分析研究領域, 状態分析ユニット長 (60353992)
SHORIGANE Akemi  農業・食品産業技術総合研究機構, 食品総合研究所・食品分析研究領域, 特別研究員 (40414495)
YAMAGUCHI Masami  東京農業大学, 農学部, 教授 (80355370)
Project Period (FY) 2006 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥46,930,000 (Direct Cost: ¥36,100,000、Indirect Cost: ¥10,830,000)
Fiscal Year 2009: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
Fiscal Year 2008: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
Fiscal Year 2007: ¥11,830,000 (Direct Cost: ¥9,100,000、Indirect Cost: ¥2,730,000)
Fiscal Year 2006: ¥20,150,000 (Direct Cost: ¥15,500,000、Indirect Cost: ¥4,650,000)
Keywords環境 / 気候変動 / 温暖化 / 休眠生理 / 果樹 / MRI / レドックス / 水チャネル / ネクロシス
Research Abstract

The transition from bud endodormancy state was characterized by a significant increasing on glutathione content in dormant bud tissues. The GSH accumulation in the buds was coincided with major increases in transcript patterns of genes GSH1 and GSH2 and redox regulating enzyme genes such as the alternative oxidase (AOX). According to the findings by MRI observation, a gradual increase in water content to that required for peach dormant bud activity at the end of endodomancy was resulted from inter- and intracell communication through membrane transport properties of aquaporins. And also, the mixed buds of Japanese pear subjected to low chilling accumulation showed low water mobility and water content especially in scales. In contrast, increased water mobility and relative content were observed in specific portions, which was more accentuated in the mixed buds of plants grown for several seasons under mild winter conditions, resulting in the necrosis of floral primordia, and the development of new inflorescences which corresponds an irregular floral form.

Report

(6 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report   Self-evaluation Report ( PDF )
  • 2007 Annual Research Report
  • 2006 Annual Research Report
  • Research Products

    (37 results)

All 2010 2009 2008 2007 2006

All Journal Article (18 results) (of which Peer Reviewed: 16 results) Presentation (19 results)

  • [Journal Article] Influence of chilling accumulation time on "Flower bud abortion" occurrence in Japanese pear grown under mild winter conditions2010

    • Author(s)
      Yamamoto, R. R. Y. Sekozawa, S. Sugaya, H. Gemma
    • Journal Title

      Acta Hortic (In press)

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] The glutathione accumulation on mixed bud tissues during the dormancy of Japanese pear2010

    • Author(s)
      Zanol, G. C., Y. Sekozawa, S. Sugaya, H. Gemma
    • Journal Title

      Acta Hortic (In press)

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Floral primordial necrosis incidence in mixed buds of Japanese pear (Pyrus pyrifolia (Burm.) Nakai var. culta) 'Housui' grown under mild winter conditions and the possible relation with water dynamics2010

    • Author(s)
      Yamamoto, R. R., A. K. Horigane, M. Yoshida, Y. Sekozawa, S. Sugaya, H. Gemma
    • Journal Title

      J.Japan.Soc.Hort.Sci. (Inpress)

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Floral primordial necrosis incidence in mixed buds of Japanese pear(Pyruspyrifolia(Burm.)Nakai var.culta)'Housui'grown under mild winter conditions and the possible relation with water dynamics2010

    • Author(s)
      Yamamoto, R.R., Horigane, A.K., Yoshida, M., Sekozawa, Y., Sugaya, S., Gemma, H.
    • Journal Title

      J.Japan.Soc.Hort.Sci (In press)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Differential adaptation of high- and low-chill dormant peaches in winter through aquaporin gene expression and soluble sugar content2009

    • Author(s)
      Yooyongwech, S., Y. Sekozawa, S. Sugaya, H. Gemma
    • Journal Title

      Plant Cell Reports. 28

      Pages: 1709-1715

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Differential adaptation of high- and low-chill dormant peaches in winter through aquaporin gene expression and soluble sugar content2009

    • Author(s)
      Yooyongwech, S., Sekozawa, Y., Sugaya, S., Gemma, H.
    • Journal Title

      Plant Cell Reports 28

      Pages: 1709-1715

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Influence of chilling accumulation time on "Flower bud abort ion" occurrence in Japanese pear grown under mild winter conditions2009

    • Author(s)
      Yamamoto, R. R. Sekozawa, Y., Sugaya,S and Gemma, H.
    • Journal Title

      Acta Horti culturae (In press)

    • Related Report
      2008 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] The glutathione accumu-Lation on mixed bud tissues during the dormancy of Japanese pear2009

    • Author(s)
      Zanol, G. C., Sekozawa, Y., Sugaya, S. and Gemma, H.
    • Journal Title

      (In press)

    • Related Report
      2008 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Effect of oscillating temperature on the expression of two aquaporin genes (Pp-δTIP1, Pp-PIP2) involved in regulating intercellular water status in flower buds of peach2008

    • Author(s)
      Yooyongwech, S., A. Horigane, M. Yoshida, Y. Sekozawa, S. Sugaya, H. Gemma
    • Journal Title

      J.Hort.Sci.Biotech. 83

      Pages: 784-790

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Changes in aquaporin gene expression and magnetic resonance imaging of water status in peach tree flower buds during dormancy2008

    • Author(s)
      Yooyongwech, S., A. Horigane, M. Yoshida, M, Yamaguchi, Y. Sekozawa, S. Sugaya, H. Gemma
    • Journal Title

      Physiol.Plant. 134

      Pages: 522-533

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Changes in aquaporin gene expression and magnetic resonance imaging of water status in peach tree flower buds during dormancy2008

    • Author(s)
      Yooyongwech, S., A. Horigane, M. Yoshida M. Yamaguchi, Y. Sekozawa, S. Sugaya, and H. Gemma
    • Journal Title

      Physiol. Plant 134

    • Related Report
      2008 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Effect of oscillating temperature on the expression of two aquaporin genes(Pp-δ TIP1, PIP2)involved in regulating intercellular water status in flower buds of peach2008

    • Author(s)
      Yooyongwech, S., A. Horigane, M. Yoshida Y. Sekozawa, S. Sugaya, and H. Gemma
    • Journal Title

      J. Hort. Sci. Biotech 83

    • Related Report
      2008 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Changes in aquaporin gene expression and magnetic resonance imaging of water status in peach tree flower buds during dormancy.2008

    • Author(s)
      Yooyongwech, S., A. Horigane, M. Yoshida, M, Yamaguchi, Y.Sekozawa, S. Sugaya, and H. Gemma
    • Journal Title

      Physiol. Plant. 134

      Pages: 522-533

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effect of oscillating temperature on the expression of two aquaporin genes (Pp-(8TIP1, Pp-PIP2 ) involved in regulating intercellular water status in flower buds of peach.2008

    • Author(s)
      Yooyongwech, S., A.Horigane , M. Yoshida, Y. Sekozawa, S. Suzava. and H. Gemma
    • Journal Title

      J. Hort. Sci. Biotech. 83

      Pages: 784-790

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Influence of chilling accumulation time on"Flower bud abortion"occurrence in lJapanese pear grown under mild winter conditions2008

    • Author(s)
      Yamamoto, R. R. Sekozawa, Y., Sugaya, S. and Gemma, H.
    • Journal Title

      Acta Horticulturae (In press)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The glutathione accumulation on mixed bud tissues during the dormancy of Japanese pear2008

    • Author(s)
      Zanol, G. C., Sekozawa, Y., Sugaya, S. and Gemma, H.
    • Journal Title

      Acta Horticulturae (In press)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] ニホンナシの休眠芽の自発休眠打破にともなうシアン耐性呼吸末端酸化酵素(AOX)活性とその遺伝子発現の変化2007

    • Author(s)
      Zanol Geni Carmen, 瀬古澤由彦, 菅谷純子, 弦間洋
    • Journal Title

      園芸学研究 6(別1)

      Pages: 336-336

    • NAID

      10024387676

    • Related Report
      2006 Annual Research Report
  • [Journal Article] MRIによるモモの休眠芽の水の動態解析(ADC)と水チャネル遺伝子の発現解析2006

    • Author(s)
      スラウット ユーヨンウェーシ, 掘金明美, 吉田充, 山口正己, 瀬古澤由彦, 菅谷純子, 弦間洋
    • Journal Title

      園芸学雑誌 75(別2)

      Pages: 136-136

    • NAID

      10019588138

    • Related Report
      2006 Annual Research Report
  • [Presentation] ニホンナシ'豊水'における暖冬条件下で発生する発芽不良・花芽のネクロシス現象に関連する休眠芽中の水の動態について2010

    • Author(s)
      山本ロブソン隆・堀金明美・吉田充・瀬古澤由彦・菅谷純子・弦間洋
    • Organizer
      園芸学会
    • Place of Presentation
      日本大学生物資源科学部
    • Related Report
      2009 Final Research Report
  • [Presentation] ニホンナシ'豊水'における暖冬条件下で発生する発芽不良・花芽のネクロシス現象に関連する休眠芽中の水の動態について2010

    • Author(s)
      山本ロブソン隆・堀金明美・吉田充・瀬古澤由彦・菅谷純子・弦間洋
    • Organizer
      園芸学会平成22年度春季大会
    • Place of Presentation
      日本大学生物資源科学部
    • Related Report
      2009 Annual Research Report
  • [Presentation] Effect of hydrogen cyanamide on dormancy release, budbreak and fruiting in Japanese pear2009

    • Author(s)
      Zanol. G. C., Y. Sekozawa, S. Sugaya, H. Gemma
    • Organizer
      11^<th> Int. Sym. on Plant Bioregulators in Fruit Production
    • Place of Presentation
      Italy
    • Related Report
      2009 Final Research Report
  • [Presentation] Effect of hydrogen cyanamide on dormancy release, budbreak and fruiting in Japanese pear2009

    • Author(s)
      Zanol.G.C., 瀬古澤由彦, 菅谷純子, 弦間洋
    • Organizer
      11^<th> Int.Sym.on Plant Bioregulators in Fruit Production
    • Place of Presentation
      Bologna Univ.Italy
    • Related Report
      2009 Annual Research Report
  • [Presentation] Elucidation of dormancy on temperate fruit trees with approaches of aquaporin gene expression and MRI observation in relation to water movement in the dormant bud2008

    • Author(s)
      Gemma, H.
    • Organizer
      The first Asian Horticultural Congress
    • Place of Presentation
      Korea
    • Year and Date
      2008-12-11
    • Related Report
      2009 Final Research Report
  • [Presentation] 変温条件下におかれたモモ花芽におけるアクアポリン(水チャネル)遺伝子の発現2008

    • Author(s)
      Yooyongwech, S・堀金明美・吉田充・瀬古澤由彦・菅谷純子・弦間洋洋
    • Organizer
      園芸学会平成20 年度秋季大会
    • Place of Presentation
      三重大学生物資源学部
    • Year and Date
      2008-12-11
    • Related Report
      2008 Self-evaluation Report
  • [Presentation] Elucidation of dormancy on temperate fruit trees with approaches of aquaporin gene Expression and MRI observation in relation to water movement in the dormant bud2008

    • Author(s)
      Gemma, H.
    • Organizer
      The first Asian Horticultural Congress
    • Place of Presentation
      韓国・済州島国際会議場
    • Year and Date
      2008-12-11
    • Related Report
      2008 Self-evaluation Report
  • [Presentation] Elucidation of dormancy on temperate fruit trees with approaches of aquaporin gene expression. and MRI observation in relation to water movement in the dormant bud2008

    • Author(s)
      Gemma, H.
    • Organizer
      The first Asian Horticultural Congress
    • Place of Presentation
      韓国・済州島国際会議場
    • Year and Date
      2008-12-11
    • Related Report
      2008 Annual Research Report
  • [Presentation] 変温条件下におかれたモモ花芽におけるアクアポリン (水チャネル) 遺伝子の発現2008

    • Author(s)
      Yooyongwech, S・堀金明美・吉田充・瀬古澤由彦・菅谷純子・弦間洋
    • Organizer
      園芸学会
    • Place of Presentation
      三重大学
    • Year and Date
      2008-09-28
    • Related Report
      2009 Final Research Report
  • [Presentation] 変温条件下におかれたモモ花芽におけるアクアポリン(水チャネル)遺伝子の発現2008

    • Author(s)
      Yooyongwech, S、堀金明美、吉田充、瀬古澤由彦、菅谷純子、弦間洋
    • Organizer
      園芸学会平成20年度秋季大会
    • Place of Presentation
      三重大学生物資源学部
    • Year and Date
      2008-09-28
    • Related Report
      2008 Annual Research Report
  • [Presentation] Water behavior and water channel gene expression in vegetative and flower dormant buds of peaches having Different chilling-requirement during winter period2007

    • Author(s)
      Yooyongwech S, Horigane AK, Yoshida M, Yamaguchi M, Sugaya S, Gemma H.
    • Organizer
      The 8^<th> International Conference on Magnetic Resonance Microscopy
    • Place of Presentation
      宇都宮大学
    • Year and Date
      2007-08-22
    • Related Report
      2008 Self-evaluation Report
  • [Presentation] Induction of water pump by fluctuating Temperature for flower budburst of peach Observed by aquaporin gene expression and MRI2007

    • Author(s)
      Yooyongwech S, Horigane AK, Yoshida M, Sugaya S, Gemma H.
    • Organizer
      The 5^<th> International Conference of Aquaporin
    • Place of Presentation
      奈良先端科学大学院大学
    • Year and Date
      2007-07-14
    • Related Report
      2008 Self-evaluation Report
  • [Presentation] ニホンナシの休眠芽の自発休眠打破にともなうシアン耐性呼吸未端酸化酵素(AOX)2007

    • Author(s)
      Zanol Geni Carmen・瀬古澤由彦・菅谷純子・弦間洋
    • Organizer
      活性とその遺伝子発現の変化
    • Place of Presentation
      京都テルサ
    • Year and Date
      2007-03-25
    • Related Report
      2008 Self-evaluation Report
  • [Presentation] Water behavior and water channel gene expression in vegetative and flower dormant buds of peaches having different chilling requirement during winter period2007

    • Author(s)
      Yooyongwech S, A. K. Horigane, M. Yoshida, M. Yamaguchi, S. Sugaya, H. Gemma
    • Organizer
      The 8th International Conference on Magnetic Resonance Microscopy
    • Place of Presentation
      宇都宮大学
    • Related Report
      2009 Final Research Report
  • [Presentation] Induction of water pump by fluctuating temperature for flower budburst of peach observed by aquaporin gene expression and MRI2007

    • Author(s)
      Yooyongwech S, A. K. Horigane, M. Yoshida, S. Sugaya, H. Gemma
    • Organizer
      The 5^<th> International Conference of Aquaporin
    • Place of Presentation
      奈良先端科学大学院大学
    • Related Report
      2009 Final Research Report
  • [Presentation] ニホンナシの休眠芽の自発休眠打破にともなうシアン耐性呼吸末端酸化酵素 (AOX) 活性とその遺伝子発現の変化2007

    • Author(s)
      Zanol Geni Carmen・瀬古澤由彦・菅谷純子・弦間洋
    • Organizer
      園芸学会
    • Place of Presentation
      京都テルサ (京都市)
    • Related Report
      2009 Final Research Report
  • [Presentation] Induction of water pump by fluctuating temperature for flower budburst of peach observed by aquaporin gene expression and MRI.2007

    • Author(s)
      Yooyongwech S, Horigane AK, Yoshida M, Sugaya S, Gemma H.
    • Organizer
      The 5th International Confbrence of Aquaporin
    • Place of Presentation
      奈良先端科学大学院大学
    • Related Report
      2007 Annual Research Report
  • [Presentation] Water behavior and water channel gene expression in vegetative and flower dorm ant buds of peaches having different chilling-requirement during willter period.2007

    • Author(s)
      Yooyongwech S, Horigane AK, Yoshida M, Yamaguchi M, sugaya S, Gemma H,
    • Organizer
      The 8th International Conference on Magnetic Resonance Microscopy.
    • Place of Presentation
      宇都宮大学
    • Related Report
      2007 Annual Research Report
  • [Presentation] MRIによるモモの休眠芽の水の動態解析(ADC)と水チャネル遺伝子の発現解析2006

    • Author(s)
      スラウット ユーヨンウェーシ・堀金明美・吉田充・山口正己・瀬古澤由彦・菅谷純子・弦間洋
    • Organizer
      園芸学会
    • Place of Presentation
      長崎大学
    • Related Report
      2009 Final Research Report

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Published: 2006-04-01   Modified: 2016-04-21  

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