Production of novel functional lipids by manifold application of microbial capacity

• Project/Area Number: 18208009
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: Kyoto University
• Principal Investigator: SHIMIZU Sakayu Kyoto University, 大学院・農学研究科, 教授 (70093250)
• Co-Investigator(Kenkyū-buntansha): KATAOKA Michihiko 京都大学, 大学院・農学研究科, 准教授 (90252494) ; OGAWA Jun 京都大学, 微生物科学寄附研究部門, 教授 (70281102) ; HAGISHITA Tairo 京都大学, 大学院・農学研究科, 准教授 (20432346) ; HIBI Makoto 京都大学, 大学院・農学研究科, 助教 (30432347) ; KISHINO Shigenobu 京都大学, 大学院・農学研究科, 助教 (40432348) ; 櫻谷 英治 京都大学, 農学研究科, 助教 (10362427)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥47,710,000 (Direct Cost: ¥36,700,000、Indirect Cost: ¥11,010,000); Fiscal Year 2008: ¥11,960,000 (Direct Cost: ¥9,200,000、Indirect Cost: ¥2,760,000); Fiscal Year 2007: ¥14,820,000 (Direct Cost: ¥11,400,000、Indirect Cost: ¥3,420,000); Fiscal Year 2006: ¥20,930,000 (Direct Cost: ¥16,100,000、Indirect Cost: ¥4,830,000)
• Keywords: 発酵生産 / 共役リノール酸 / 乳酸菌 / 高度不飽和脂肪酸 / デスモステロール / 共役高度不飽和脂肪酸 / Clostridium / 嫌気的反応 / 脂肪酸の飽和化 / Mortierella / 分子育種 / 変異株 / アラキドン酸 / 宿主ベクター系 / モルティエレラ / 糸状菌 / ヒドロキシ脂肪酸

Research Abstract

乳酸菌は機能性が注目されている共役リノール酸をつくる。ここでは、リノール酸から共役リノール酸への変換に関わる一連の酵素系を解明し、乳酸菌による共役リノール酸生産を確立した。さらに、油糧微生物の遺伝子操作や変異処理により、機能性が高い高度不飽和脂肪酸やステロール類の発酵生産技術を確立した。これら機能性脂質は、医薬・健康食品としての利用が多いに期待されるものである。

Research Products

• [Journal Article] Selective production of cis-9, trans-11 isomer of conjugated linoleic acid from trans-vaccenic acid methyl ester by Delacroixia coronata (2009)
  • Author(s): 安藤晃規、小川順、杉本聡、岸野重信、櫻谷英治、横関健三、清水昌
  • Journal Title: J. Appl. Microbiol. 106(5) Pages: 1697-1704
  • Peer Reviewed
• [Journal Article] Metabolic diversity in biohydrogenation of polyunsaturated fatty acids by lactic acid bacteria involving conjugated fatty acid production (2009)
  • Author(s): 岸野重信、小川順、横関健三、清水昌
  • Journal Title: Appl. Microbiol. Biotechnol
  • NAID: 120001624125
  • Peer Reviewed
• [Journal Article] Selective production of cis-9, trans-11 isomer of conjugated linoleic acid from trans-vaccenic acid methyl ester by Delacroixia coronata (2009)
  • Author(s): Akinori Ando
  • Journal Title: J. Appl. Microbiol.
  • Peer Reviewed
• [Journal Article] Metabolic diversity in biohydrogenation of polyunsaturated fatty acids by lactic acid bacteria involving conjugated fatty acid production (2009)
  • Author(s): Shigenobu Kishino
  • Journal Title: Addl. Microbiol. Biotechnol.
  • NAID: 120001624125
  • Peer Reviewed
• [Journal Article] Accumulation of prenyl alcohols by terpenoid biosynthesis inhibitors in various microorganisms (2008)
  • Author(s): Masayoshi Muramatsu、Chikara Ohto、Shusei Obata、櫻谷英治、清水昌
  • Journal Title: Appl. Microbiol. Biotechnol 80(4) Pages: 589-595
  • Peer Reviewed
• [Journal Article] 酵素法による共役脂肪酸の合成 (2008)
  • Author(s): 小川順、岸野重信、横関健三、清水昌
  • Journal Title: ファインケミカル 37 Pages: 18-27
  • Peer Reviewed
• [Journal Article] Accumulation of prenyl alcohols by terpenoid biosynthesis inhibitors in various microorganisms (2008)
  • Author(s): Masayoshi Muramatsu
  • Journal Title: Addl. Microbiol. Biotechnol. 80 Pages: 589-595
  • Peer Reviewed
• [Journal Article] 酵素法による共役脂肪酸の合成 (2008)
  • Author(s): 小川 順
  • Journal Title: ファインケミカル 37 Pages: 18-27
• [Journal Article] Identification of a sterol Δ7 reductase gene involved in desmosterol biosynthesis in Mortierella alpina 1S-4、Appl. Environ (2007)
  • Author(s): 張朔、櫻谷英治、清水昌
  • Journal Title: Microbiol 73(6) Pages: 1736-1741
  • Peer Reviewed
• [Journal Article] Identification of a novel bifunctional Δ12/Δ15 fatty acid desaturase from a basidiomycete (2007)
  • Author(s): 張朔、櫻谷英治、伊東久仁、清水昌
  • Journal Title: Coprinus cinereus TD#822-2、FEBS Lett 581(2) Pages: 315-319
  • Peer Reviewed
• [Journal Article] Identification of a sterol Δ7 reductase gene involved in desmosterol biosynthesis in Mortierella alpina 1S-4 (2007)
  • Author(s): Shuo Zhang
  • Journal Title: Appl. Environ. Microbiol. 73 Pages: 1736-1741
  • Peer Reviewed
• [Journal Article] Identification of a novel bifunctional Δ12/Δ15 fatty acid desaturase from a basidiomycete, Coprinus cinereus TD#822-2 (2007)
  • Author(s): Shuo Zhang
  • Journal Title: FEBS Lett. 581 Pages: 315-319
  • Peer Reviewed
• [Journal Article] Identification and production of n-8 odd-numbered polyunsaturated fatty acids by a Δ12 desaturation-defective mutant of Mortierella alpina 1S-4 (2006)
  • Author(s): 張朔、櫻谷英治、清水昌
  • Journal Title: Lipids 41(6) Pages: 623-626
  • Peer Reviewed
• [Journal Article] Functional characterization of Δ9 and ω9 desaturase genes in Mortierella alpina 1S-4 and its derivative mutants (2006)
  • Author(s): 阿部孝宏、櫻谷英治、浅野貴弘、金丸博幸、清水昌
  • Journal Title: Appl. Microbiol. Biotechnol 70(6) Pages: 711-719
  • Peer Reviewed
• [Journal Article] Expression of Δ6, Δ5 desaturase and GLELO elongase genes from Mortierella alpina for production of arachidonic acid in soybean[Glycine max(L.)Merrill]seeds (2006)
  • Author(s): Ren Chen、Keisuke Matsu、Miyuki Ogawa、Mieko Oe、落合美佐、河島洋、櫻谷英治、清水昌、Masao Ishimoto、Makoto Hayashi、Yoshikatsu Murooka、Yoshikazu Tanaka
  • Journal Title: Plant Science 170 Pages: 399-406
  • Peer Reviewed
• [Journal Article] Screening and industrial application of unique microbial reactions involved in nucleic acid and lipid metabolisms (2006)
  • Author(s): 小川順、宋子良、岸野重信、李青山、堀之内伸行、清水昌
  • Journal Title: Biosci. Biotechnol. Biochem 70(3) Pages: 574-582
  • NAID: 10018533069
  • Peer Reviewed
• [Journal Article] Identification and production of n-8 odd-numbered polyunsaturated fatty acids by a Δ12 desaturase-defective mutant of Mortierella (2006)
  • Author(s): Shuo Zhang
  • Journal Title: Lipids 41 Pages: 623-626
• [Journal Article] Expression of Δ6, Δ5 desaturase and GLELO elongase genes from Mortierella alpina for production of arachidonic acid in soybean [Glycine max (L.) Merrill] seeds (2006)
  • Author(s): Ren Chen
  • Journal Title: Plant Science 170 Pages: 399-406
• [Journal Article] Functional characterization of Δ9 and ω9 desaturase genes in Mortierella alpina 1S-4 and its derivative mutants (2006)
  • Author(s): Takahiro Abe
  • Journal Title: Appl. Microbial. Biotechnol. 70 Pages: 711-719
• [Journal Article] Screening and Industrial Application of Unique Microbial Reactions Involved in Nucleic Acid and Lipid Metabolisms (2006)
  • Author(s): Jun Ogawa
  • Journal Title: Biosci. Biotechnol. Biochem. 70 Pages: 574-582
  • NAID: 10018533069
• [Presentation] 油糧微生物の代謝工学と機能性脂質生産への利用 (2008)
  • Author(s): 櫻谷英治、野重信、小川順、横関健三、○清水昌
  • Organizer: 第60回日本生物工学会大会
  • Place of Presentation: 宮城(仙台)(3S2a05)
  • Year and Date: 2008-08-29
• [Presentation] 油糧微生物の代謝工学と機能性脂質生産への利用 (2008)
  • Author(s): Sakayu Shimizu
  • Organizer: 日本生物工学会大会
  • Place of Presentation: 宮城県(仙台)
  • Year and Date: 2008-08-29
• [Presentation] Unique microbial reactions useful for conjugated fatty acid production (2007)
  • Author(s): ○小川順、岸野重信、横関健三、清水昌
  • Organizer: 2nd International Congress on Conjugated Linoleic Acid
  • Place of Presentation: イタリア(Cagliari)
  • Year and Date: 2007-09-21
• [Presentation] Unique microbial reactions useful for conjugated fatty acid production (2007)
  • Author(s): Jun Ogawa
  • Organizer: 2nd International Congress on Conjugated Linoleic Acid
  • Place of Presentation: Cagliari, Italy
  • Year and Date: 2007-09-21
• [Presentation] 微生物機能の探索・開発とバイオプロセス構築へのアプローチ (2006)
  • Author(s): ○清水昌
  • Organizer: 第58回日本生物工学会大会
  • Place of Presentation: 大阪(1A-AM2)
  • Year and Date: 2006-09-11